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1.
为对发病雏鸡群进行确诊,通过流行病学调查、病雏剖检观察及细菌分离鉴定,并对分离菌进行小白鼠接种观察,证实鸡群发生了由鼠伤寒沙门氏菌和鸡伤寒沙门氏菌混合感染引起的禽副伤寒和禽伤寒,其中鼠伤寒沙门氏菌是主要病原,感染率高于鸡伤寒沙门氏菌。药物敏感试验结果表明,分离菌对多种常见抗生素表现敏感。 相似文献
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贾兆爱 《畜牧兽医科技信息》2011,(2):36-37
仔猪副伤寒主要是由猪霍乱沙门氏菌和肠炎沙门氏菌引起的,是危害仔猪的传染病。临床表现为腹泻,严重者可出现败血症。本试验从疑似仔猪副伤寒病例中采集病料,进行菌株分离与鉴定,结果分离菌株为肠炎沙门氏菌,从而将此病例确诊为仔猪副伤寒。 相似文献
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云南曲靖市沾益乡金龙综合养猪场曾爆发了一起以四月龄以下仔猪为主的猪发病及死亡严重的疫病。经对这次疫情发生的流行病学调查、临床诊断、病理剖检及实验室细菌学检验、分离获得猪霍乱沙门氏菌病原 ,从而确诊为猪副伤寒病。猪副伤寒病的流行 ,严重地危害养猪业的发展 ,造成巨大的经济损失 ,特别是对四月龄以下的仔猪危害极大 ,此次分离获得的猪霍乱沙门氏菌菌株毒力极强 ,以致引起该猪场从四川刚购进的３８８头仔猪发病死亡３５２头 ,猪场自繁自养的仔猪发病死亡２７７头 ,育肥猪、老母猪共发病死亡２６头 ,造成经济损失十八万元之多。现… 相似文献
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仔猪副伤寒主要是由猪霍乱沙门氏茵和肠炎沙门氏菌引起的,主要是危害仔猪的传染病。主要临床症状表现为腹泻,严重者可出现败血症。本试验从疑似仔猪副伤寒病例中采集病料,进行分离菌株与鉴定,结果分离菌株为肠炎沙门氏菌,从而将此病例确诊为仔猪副伤寒。 相似文献
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丘陵地区生猪养殖很容易受到亚热带气候的影响,入春时节气候变化较多,若养殖管理跟不上变化,易引发细菌性疾病,2～4月龄仔猪最易感染沙门氏菌,成年猪发病较少。猪副伤寒又称猪沙门氏菌病,主要是由猪霍乱和猪伤寒沙门氏菌引起,急性副伤寒会造成仔猪死亡,但临床较多的是慢性副伤寒,病猪 相似文献
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新疆地区鸡伤寒白痢沙门氏菌病的调查 总被引：5，自引：0，他引：5
对新疆地区鸡沙门氏菌病进行了病原学、血清学和流行病学调查，结果表明分离的246株细菌与鸡白痢鸡伤少门氏菌诊断血清呈阳性反应；对15株鸡沙门氏菌进行抗原结构分析，表明8株为标准型鸡白痢伤寒沙门氏菌，7株为中间型鸡沙门氏菌，对其中86株菌的生化鉴定表明符合鸡白痢鸡伤寒沙门氏菌的生化特性。该病在我区的发病死亡率为1％－25％，发病严重的鸡场为50％－75％。该病除了单一感染外，还与鸡大肠杆菌、葡萄球菌、鸡球虫病混合感染。 相似文献
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《中国畜牧兽医文摘》2016,(7)
<正>仔猪副伤寒又称猪沙门氏菌病,主要是由猪霍乱和猪伤寒沙门氏菌引起的仔猪高热传染病,多发生在1~4月龄的仔猪,急性病例表现败血症,慢性病例为坏死性肠炎或卡他性或干酪性肺炎,给养猪业带来巨大经济损失。2015年4月中旬我县某生猪养殖户发生猪食欲不振、精神萎靡、耳尖、四肢末端及腹部发绀,伴有黄色下痢并死亡的病例通过流行病学调查、临床症状、剖检变化、实验室检验确诊为仔猪副伤寒救治情况如下。 相似文献
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猪副伤寒病,又称猪沙门氏菌病,主要是由猪伤寒沙门氏菌、猪霍乱沙门氏菌、肠炎沙门氏菌等引起的一种慢性传染病。沙门氏菌是革兰氏阴性杆菌,无荚膜和芽孢,抗原式相似,生化特性复杂,广泛存在自然界中,并且血清型达2500种以上,此菌为条件性致病菌,污染源主要来自于患病猪 相似文献
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R C Anderson K J Genovese R B Harvey L H Stanker J R DeLoach D J Nisbet 《Journal of veterinary diagnostic investigation》2000,12(3):257-260
In the United States, swine salmonellosis is most often attributed to infections by Salmonella serovar choleraesuis. As a host-adapted pathogen rarely found in nonswine sources, S. choleraesuis is thought to be spread primarily via horizontal transmission, with carrier animals playing an important role. Little has been reported regarding infection of neonatal piglets, particularly regarding their potential to become carriers. Evidence reported herein demonstrates that piglets experimentally infected by S. choleraesuis at 2 days of age were capable of shedding the pathogen for up to 85 days postinfection, at which time the study was concluded. This study also presents findings supporting the use of GN-Hajna as a preenrichment medium for the isolation of S. choleraesuis. 相似文献
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B P Wilcock 《Canadian journal of veterinary research》1979,43(2):200-206
Twenty colostrum-fed piglets from three sows were separated from the sows 24 hours after birth and were randomly divided into five groups of four piglets each. Every piglet in each of four test groups was orally inoculated with about 10(10) colony forming units of Salmonella typhimurium, Salmonella choleraesuis var Kunzendorf or one of two isolates of Klebsiella pneumoniae. One group served as uninoculated controls. Piglets infected with K. pneumoniae developed severe diarrhea beginning about 12 hours after inoculation. They became dehydrated and weak but continued to drink. There were no morphological alterations in intestinal mucosa when piglets were killed and necropsied 48 or 72 hours after inoculation. Klebseilla pneumoniae was isolated from intestine and feces but not from liver or spleen. Piglets inoculated with S. choleraesuis became lethargic and disinterested in food by 24 hours after inoculation. Diarrhea developed by 48 hours after inoculation. Lesions at necropsy 60 or 72 hours postinoculation were subcutaneous edema, mesenteric lymphadenitis, diffuse intestinal superficial mucosal necrosis with villous atrophy, and focal deep ulceration in the ileum. Salmonella choleraesuis was isolated from all segments of intestine and from feces, liver and spleen. Piglets inoculated with S. typhimurium developed a relatively mild diarrheal disease with lesions similar to those with S. choleraesuis infection but less severe. The inoculated organism was recovered from all areas of intestine and from feces, liver and spleen. Serum from infected and control piglets had high (greater than 1:256) agglutinating titres against S. typhimurium but low titres (0 to 1:8) against S. choleraesuis. The agglutinins were assumed to originate from colostral antibodies. 相似文献
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本研究通过采用高通量测序、平板筛选、16S rDNA序列分析、特异性碳源培养和牛津杯法,从哺乳期健康仔猪的粪便中筛选分离和鉴定乳杆菌,并进行其利用表乳糖生长特性以及抑制仔猪腹泻相关致病菌活性的试验研究,探索表乳糖定向调控仔猪肠道乳杆菌增殖的可能性。结果表明:从7~10日龄哺乳期健康仔猪的粪便样品中分离出9株乳杆菌,其中唾液乳杆菌、卷曲乳杆菌、淀粉乳杆菌具有较强利用表乳糖的能力,在以2%表乳糖为唯一碳源的MRS培养基中显现出良好的生长态势,而且其培养上清液对大肠杆菌(猪源)、猪霍乱沙门氏菌、单核细胞增生李斯特氏菌、宋氏志贺氏菌以及金黄色葡萄球菌具有较好的抑菌活性。 相似文献
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为了对某猪场病死仔猪的死亡病因进行调查,本试验采集病料进行研究,采用鉴别培养基分离、生化特性鉴定、PCR扩增细菌16S rRNA基因及测序分析等方法对样品中存在的细菌进行分离鉴定,结果显示分离菌为李斯特杆菌。采用标准K-B纸片法对分离菌株进行20种抗菌药物的药敏试验,并对其生长曲线进行测定。结果显示,此菌株对青霉素G、磷霉素、阿莫西林等药物高敏,而对呋喃唑酮等药物耐药。37 ℃培养6~14 h后细菌处于增殖期。本研究为中国仔猪李斯特杆菌病细菌学检测及治疗提供了科学依据。 相似文献
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LI Hao-xin DUAN Gang XIANG Xun ZU Fei YANG Zhi-yuan ZHU Qi DAI Fei-yan CHANG Hua 《中国畜牧兽医》2015,42(7):1883-1889
The assay was aimed to investigate the cause of death of diseased and dead piglets,we collected samples and identified by growth characteristics of bacteria in different mediums,biochemistry identification,PCR amplification of 16S rRNA genes and sequence analysis.The results showed that the isolate was Listeria.Drug sensitive test of the isolate to 20 antibiotics was detected.The results showed that this bacterium was highly sensitive to penicillin G,fosfomycin and amoxicillin and so on;However,it was resistant to furazolidone and so on.The growth curve of Listeria showed that the bacterium was in the proliferation period when 37 ℃ cultured 6 to 14 h.The results of this study provided scientific bases for detection and treatment of listeriosis in China. 相似文献
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M B Roof T T Kramer J P Kunesh J A Roth 《American journal of veterinary research》1992,53(8):1333-1336
Seventy-five pigs from 4 facilities were examined for Salmonella choleraesuis by use of bacteriologic culture of feces, blood, WBC (buffy coat), mononuclear leukocytes, and neutrophils. The organism was isolated from 0 of 75 fecal samples, compared with isolation from 39 of 75 purified neutrophil preparations. Of the pigs that did not have Salmonella isolated from feces or blood, but had S choleraesuis isolated from neutrophils, 6 were further examined. These pigs from 2 groups again had culture performed at least 3 successive times to test for repeatability and to determine optimal number of neutrophils required for Salmonella isolation. These same pigs were euthanatized and necropsied. Nineteen tissue specimens from each pig were obtained for culture, but S choleraesuis was isolated only from neutrophil samples. Results indicate that neutrophils may contribute to the carrier state in pigs and should be cultured when attempting to identify S choleraesuis carrier swine. 相似文献
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肺炎克雷伯氏菌强毒株的分离鉴定及16-23SrRNAITS序列分析 总被引：1，自引：1，他引：0
为确诊疑似仔猪肺炎克雷伯氏菌(K.pneumonia)感染,并研究其病原的致病性、耐药性、16-23SrRNA ITS系统进化特征,本研究从云南因肺炎、腹泻而大量死亡的仔猪中分离到1株革兰氏阴性短粗杆菌,命名为KP14013,对其进行生化鉴定、16SrRNA鉴定,研究其对小白鼠和仔猪的致病性,并对其16-23SrRNA ITS基因进行测序和遗传进化分析。结果显示,KP14013分离株生化特征与肺炎克雷伯氏菌相符,其16SrRNA与GenBank中23株肺炎克雷伯氏菌代表株之间的同源性均为99%,将KP14013鉴定为肺炎克雷伯氏菌。KP14013对小白鼠半数致死量(LD50)为3×101.8 CFU,腹腔注射3×108 CFU可使仔猪100%致死。16-23SrRNA ITS系统进化关系结果表明,KP14013与GenBank中收录的15株肺炎克雷伯氏菌形成进化树的一个分支,属于同一个亚群,它们之间的核苷酸同源性为98.4%~99.2%。本研究证实了肺炎克雷伯氏菌是该起仔猪腹泻大量死亡的病原;KP14013分离株为毒力极强菌株,具有多重耐药性,其16-23SrRNA ITS与GenBank中收录的肺炎克雷伯氏菌代表株之间核苷酸存在差异,可用于肺炎克雷伯氏菌菌株间的鉴别。 相似文献
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Adhesion activity of glyceraldehyde-3-phosphate dehydrogenase in a Chinese Streptococcus suis type 2 strain 总被引：1，自引：0，他引：1
A total of 36 streptococcal strains, including seven S. equi ssp.zooepidemicus, two S. suis type 1 (SS1), 24 SS2, two SS9, and one SS7, were tested for glyceraldehyde-3-phosphate dehydrogenase gene (gapdh). Except from non-virulent SS2 strain T1 5, all strains harboured gapdh.The gapdh of Chinese Sichuan SS2 isolate ZY05719 and Jiangsu SS2 isolate HA9801 were sequenced and then compared with published sequences in the GenBank.The comparison revealed a 99.9 % and 99.8 % similarity of ZY05719 and HA9801, respectively, with the published sequence. Adherence assay data demonstrated a significant ((p<0.05)) reduction in adhesion of SS2 in HEp-2 cells pre-incubated with purified GAPDH compared to non pre-incubated controls, suggesting the GAPDH mediates SS2 bacterial adhesion to host cells. 相似文献