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1.
为了研究BDNF与TrkB蛋白在小鼠发情周期和妊娠早期子宫、输卵管及着床前胚胎中的表达规律,试验采用免疫组织化学方法检测BDNF与TrkB蛋白在子宫和输卵管中的表达,采用免疫荧光技术检测着床前不同发育阶段胚胎中蛋白的表达,并利用图像分析软件对两种蛋白在着床前胚胎中的表达强度进行定量分析。结果表明:BDNF与TrkB蛋白在发情周期及妊娠3.5天和4天子宫腔上皮、子宫腺上皮和血管内皮细胞中表达,在发情周期子宫内膜固有层基质细胞中不表达,但在妊娠3.5天和4天子宫蜕膜细胞中表达,发情前期、发情期和发情后期BDNF蛋白的表达强度高于发情间期;BDNF与TrkB蛋白在妊娠2天输卵管黏膜上皮和血管内皮细胞中表达;两种蛋白在着床前胚胎各发育阶段都有表达,在囊胚期达到最高水平。说明BDNF和TrkB蛋白通过旁分泌或自分泌途径对子宫、输卵管尤其是着床前胚胎发育发挥调控作用。 相似文献
2.
试验旨在研究核蛋白1(nuclear protein 1,Nupr1)mRNA在小鼠早期妊娠子宫中的表达,探讨Nupr1与小鼠胚胎着床的相关性。通过建立小鼠早期妊娠模型、假孕妊娠模型、延迟着床及激活模型、人工蜕膜化模型和激素处理模型,采用原位杂交的方法检测Nupr1mRNA在小鼠各种模型子宫组织中的定位表达情况,并应用实时荧光定量PCR法检测早期妊娠模型和假孕妊娠模型中Nupr1mRNA的相对表达量。结果显示,Nupr1mRNA在小鼠早期妊娠第1~4天子宫的腔上皮和腺上皮表达,第5~8天表达于蜕膜区域;假孕妊娠第1~5天,Nupr1mRNA主要表达于小鼠子宫腔上皮和腺上皮;延迟着床模型中信号表达于在小鼠子宫的腔上皮和腺上皮,与正常妊娠第4天结果相似;延迟激活模型中信号表达于蜕膜区,与早期妊娠第5天表达结果相似;人工蜕膜化模型中信号表达于蜕膜区,而蜕膜对照组中信号表达于腔上皮和腺上皮;17β-雌二醇(oestrogen,E2)处理组信号表达于腔上皮和腺上皮,信号增强,孕酮(progesterone,P4)和E2共同处理表达无明显变化;实时荧光定量PCR结果显示,正常妊娠第2天Nupr1mRNA相对表达量较高,假孕妊娠第2天Nupr1mRNA相对表达量也较高。本研究结果表明,Nupr1mRNA在小鼠子宫中的表达与小鼠早期妊娠过程相关,Nupr1mRNA在腔上皮和腺上皮的表达可能受激素调节,在子宫基质中的表达与蜕膜化及活化胚泡相关。 相似文献
3.
《中国畜牧杂志》2019,(11)
为研究孕酮对奶牛子宫内膜上皮细胞Wnt/β-catenin信号通路关键蛋白表达的影响,本试验以原代奶牛子宫内膜上皮细胞为材料,添加不同浓度孕酮(0、1、3、5 ng/mL)对其培养,采用CCK-8法检测孕酮对细胞增殖的影响;免疫印迹法检测孕酮对Wnt/β-catenin信号通路关键蛋白表达的影响。结果表明:与对照组相比,添加1、3 ng/mL孕酮组奶牛子宫内膜上皮细胞增殖率提高(P>0.05),5 ng/mL孕酮组细胞增殖率降低(P>0.05);与对照组相比,1 ng/mL和3 ng/mL孕酮组Wnt/β-catenin信号通路关键蛋白β-catenin、cyclin D1和c-Myc的表达水平均升高(P<0.01),而5 ng/mL孕酮则抑制了β-catenin的表达(P<0.05),且cyclin D1和c-Myc蛋白表达较3ng/mL孕酮组有下降趋势(P>0.05)。综上,高浓度孕酮抑制了体外培养的原代奶牛子宫内膜上皮细胞Wnt/β-catenin信号通路的激活和表达,提示该信号通路参与了奶牛产后子宫复旧延迟进程,为进一步研究奶牛子宫内膜的发病机制提供思路。 相似文献
4.
应用免疫荧光技术以生物素化的荆豆素-Ⅰ(UEA -Ⅰ)为探针,检测其结合位点α-L-岩藻糖在小鼠妊娠1～8 d、假孕1～6 d及发情周期子宫中的表达情况,并以摘除卵巢的小鼠为模型观察雌激素、孕酮对小鼠子宫α-L-岩藻糖表达的影响.结果,UEA-Ⅰ的结合位点主要位于妊娠1～4 d的子宫腔上皮及腺上皮中.在假孕小鼠,UEA-Ⅰ的结合位点主要位于假孕1～2 d的子宫腔上皮及腺上皮中,假孕3～4 d时在这些部位的结合逐渐减少或消失.另外,在发情周期各期的子宫腔上皮和腺上皮中也发现很多UEA-Ⅰ的结合部位,在发情期明显增多,当用雌激素处理时在这些部位的结合明显增加.这些表明,UEA-Ⅰ在子宫腔上皮和腺上皮上的结合位点可能与胚胎的存在与否无关, 而是受母体雌激素的调节.但胚胎着床后UEA-Ⅰ的结合位点消失,以后又在初级蜕膜中出现,表明UEA-Ⅰ的结合位点可能与胚胎着床后的蜕膜化反应有关. 相似文献
5.
为探讨脑源性神经营养因子(BDNF)及其受体酪氨酸激酶受体B(TRKB)在妊娠期山羊子宫和胎盘中的表达情况,本实验分别于妊娠15、25、65 d采集子宫和胎盘样品,免疫组织化学方法分析BDNF和TRKB蛋白的分布情况。结果表明:妊娠15 d时,BDNF和TRKB蛋白在子宫内膜上皮和腺上皮细胞中强表达,BDNF在肌层弱表达,而TRKB强表达;妊娠25 d和65 d时,2种蛋白在子宫内膜上皮和腺上皮细胞中维持强表达,同时在胎儿绒毛膜滋养层细胞中表达强烈;与妊娠15 d相比,妊娠25 d和65 d时2种蛋白均在浅层子宫腺强烈表达,深层子宫腺弱表达。综上可知,随着妊娠的进行,BDNF和TRKB蛋白的表达情况也发生变化,提示BDNF对山羊妊娠活动有一定的调节作用,与妊娠期山羊子宫和胎盘的组织结构变化相关联,可能通过与其受体TRKB的特异性结合来实现其功能。 相似文献
6.
枫泾母猪发情周期和妊娠早期外周血清孕酮含量测定 总被引：4，自引：0，他引：4
枫泾猪是中国太湖地区繁殖力高的地方猪种。本试验测定了8头枫泾和4头长白青年母猪发情周期和妊娠早期外周血清孕酮含量。这两个品种发情周期血清孕酮含量有相似的变化,峰值均出现在发情周期9～12天,枫泾猪为26.58ng/ml,长白猪为25.17ng/ml。在妊娠早期的变化也大致相同。枫泾和长白两种猪在妊娠-3～12天与发情周期-2～12天的孕酮含量变化也差不多,妊娠12天峰值枫泾为21.54ng/ml,长白为28.11ng/ml;在妊娠12天以后两种猪的孕酮含量均略有下降;16～20天后又开始回升,并维持在较高水平。发情周期和妊娠早期外周血清孕酮含量16天后有明显差异(P<0.001),可试用于猪的早期妊娠诊断。据初步测定,枫泾母猪妊娠12及16天孕酮含量与黄体数或胚胎数之间无明显相关(P>0.05)。 相似文献
7.
应用免疫荧光技术以生物素化的荆豆素-I（UEA-I）为探针，检测其结合位点a-L岩藻糖在小鼠妊振1～8d、假孕1～6d及发情周期子宫中的表达情况，并以摘除卵巢的小鼠为模型观察雌激素、孕酮对小鼠子宫a-L-岩藻糖表达的影响。结果，UEA-I的结合位点主要位于妊娠1～4d的子宫腔上及腺上皮中。在假孕小鼠，UEA-I的结合位点主要位于假孕1～2d的子宫腔上皮及腺上皮中，假孕3～4d时在这些部位的结合逐渐减少或消失。另外，在发情周期各期的子宫腔上皮和腺上皮中也发现很多UEA-I的结合部位，在发情期明显增多，当用雌激素处理时在这些部位的结合明显增加，这些表明，UEA-I在子宫腔上皮和腺上皮上的结合位点可能与胚胎的存在与否无关，而是受母体雌激素的调节。但胚胎着床后UEA-I的结合位点消失，以后又在初级蜕膜中出现，表明UEA-I的结合位点可能与胚胎着床后的蜕膜化反应有关。 相似文献
8.
外源性孕酮对妊娠小鼠着床早期子宫内膜转化生长因子β1表达的影响 总被引：1，自引：0，他引：1
应用免疫组织化学 SABC法 ,研究了外源性孕酮对妊娠小鼠着床早期子宫内膜内转化生长因子 β1 (TGFβ1 )表达的影响。结果显示 :试验组皮下注射不同剂量孕酮 (2 mg/只 ,4 mg/只 )后 ,在孕 4 d,上皮内 TGFβ1 阳性着色 ,有的腺导管内具有 TGFβ1 阳性着色 ;且随着孕酮剂量的增大 ,上皮内 TGFβ1 阳性着色由子宫腔面向基面延伸 ,而腺上皮的阳性着色则呈下降趋势 ,基质内 TGFβ1 阳性着色则呈上升趋势。在孕 5 d,上皮内 TGFβ1 阳性着色呈现随孕酮剂量增大而下降的趋势 ,腺上皮和基质的 TGFβ1 动态变化如同孕 4 d。在孕 6 d,上皮、腺上皮及基质 TGFβ1 阳性着色均与孕 5 d的变化趋势相同。由此认为 ,外源性孕酮对小鼠子宫内膜各组织 TGFβ1 蛋白表达有影响。主要表现为 ,外源性孕酮可促进基质中 TGFβ1 的产生 ,对腺上皮 TGFβ1 的表达则具有下调作用 相似文献
9.
《中国畜牧杂志》2021,(5)
核编码的甘氨酰-tRNA合成酶(Glycyl-tRNA Synthetase, GlyRS)是细胞质和线粒体蛋白质翻译所必需的,GlyRS除了在蛋白翻译中的作用外,它还参与基因转录、炎症和细胞增殖等其他生物学过程。本研究从mRNA水平和蛋白水平检测了GlyRS在小鼠早期妊娠1～8 d模型、人工诱导蜕膜化模型和体外诱导人子宫内膜基质细胞蜕膜化模型中的表达规律。结果表明:GlyRS mRNA和蛋白在小鼠早期妊娠1～4 d的腔上皮和腺上皮中表达,在小鼠早期妊娠5～8 d主要在胚胎和蜕膜区表达;随着蜕膜化程度增加,表达量逐渐增加;在人工诱导蜕膜化模型中,诱导组GlyRS mRNA和蛋白表达情况类似,表达量均明显高于对照组。在体外诱导的人子宫内膜基质细胞蜕膜化模型中,诱导组GlyRS mRNA表达量随着诱导天数的增加而增加,均显著高于对照组。以上结果表明GlyRS参与小鼠早期妊娠及子宫蜕膜化的过程。 相似文献
10.
为了探讨热休克蛋白(HSP) 27和HSP90在不同发育时期猫子宫中的表达情况,试验采用免疫组织化学技术分别检测HSP27和HSP90蛋白在5月龄、10月龄、1岁龄、1. 5岁龄、1. 5岁龄(妊娠20 d)猫子宫中的分布与表达情况。结果表明:HSP27在5月龄和10月龄猫子宫中不表达,在1岁龄、1. 5岁龄和1. 5岁龄(妊娠20 d)猫子宫中表达,不同强度阳性区域出现在腔上皮、腺上皮、固有层、血管内皮和子宫肌层中,妊娠期尿囊绒毛膜弱表达; HSP90在10月龄、1岁龄和1. 5岁龄子宫中未见表达,在5月龄和1. 5岁龄(妊娠20 d)猫子宫中表达,5月龄猫子宫腔上皮和腺上皮呈弱表达,1. 5岁龄(妊娠20 d)猫子宫腔上皮呈强表达,浅层腺上皮中等表达,深层腺上皮和尿囊绒毛膜弱表达,在子宫固有层、肌层和血管内皮未见表达。说明HSP27和HSP90蛋白在猫不同发育时期子宫中的表达具有差异性,提示两种热休克蛋白参与子宫重塑过程中子宫细胞凋亡和细胞增殖等过程。 相似文献
11.
The test was aimed to study the expression of nuclear protein 1 (Nupr1) mRNA in mouse uterus during early pregnancy.The method of in situ hybridization was used to investigate Nupr1 mRNA expression in animal models that included early pregnancy,pseudopregnancy,delayed implantation and activation,artificial decidualization and hormonal treatments.The relative expression level of Nupr1 mRNA was detected in early pregnancy and pseudopregnancy using Real-time PCR.During mouse early pregnancy,the signal of Nupr1 mRNA was detected in luminal epithelium and glandular epithelium during the 1st to 4th day and in the decidua area during the 5th to 8th day.Nupr1 mRNA was mainly expressed in the luminal epithelium and glandular epithelium of mose uterus on the 1st to 5th day of pseudopregnancy.The signal was detected in luminal epithelium and glandular epithelium of the mouse uterus in the delayed implantation,which was similar to the results of early pregnancy on the 4th day.The signal was detected in decidua in the model of delayed activation,which was similar to the results of early pregnancy on the 5th day.The expression of Nupr1 mRNA in the model of artificial decidualization was detected in decidua area.In the control of artificial decidualization the slight signal appeared in luminal epithelium and glandular epithelium of the mouse uterus.After treated with oestrogen (E2) the signal appeared in luminal epithelium and glandular epithelium of the mouse uterus,and the signal was enhanced.After treated with both of E2 and progesterone (P4), the expression of the signal was not changed significantly.Real-time PCR result showed that the relative expression on the 2nd day was higher than other days in early pregnancy and pseudopregnancy.The results indicated that the expression of Nupr1 mRNA in mouse uterus was related to the process of mouse early pregnancy.The expression of signal in luminal epithelium and glandular epithelium of the mouse uterus might be regulated by hormones.Nupr1 mRNA expression in uterine stroma was associated with decidualization and active blastocysts. 相似文献
12.
B Guo B‐C Han Z Tian X‐M Zhang L‐X Jiang J‐X Liu Z‐P Yue 《Reproduction in domestic animals》2010,45(6):e255-e259
E‐cadherin, a Ca2 + ‐dependent cell adhesion molecule, is necessary for endometrial receptivity to blastocyst implantation. The aim of this study was to investigate the differential expression of E‐cadherin in canine uterus during early pregnancy and its regulation under different conditions by in situ hybridization. E‐cadherin mRNA expression was at a low level in the glandular epithelium on days 6, 12 and 17 of pregnancy. On days 20 and 23 of pregnancy, E‐cadherin mRNA was highly expressed in the glandular epithelium surrounding the embryo, but not in the luminal epithelium and declined in villi and placenta on day 28 of pregnancy. During oestrous cycle, a moderate level of E‐cadherin mRNA expression was found in the luminal and glandular epithelium of canine uteri at oestrus stage. The same expression was also found at anoestrus stage. Progesterone slightly induced the expression of E‐cadherin mRNA in the luminal and glandular epithelium of ovariectomized canine uterus. These results suggest that E‐cadherin expression is closely related to canine implantation and can be up‐regulated by progesterone. 相似文献
13.
B Guo Z Tian B-C Han X-M Zhang Z-M Yang Z-P Yue 《Reproduction in domestic animals》2009,44(4):638-642
Hoxa10, a homeobox gene, is necessary for endometrial receptivity to blastocyst implantation. The aim of this study was to investigate the differential expression of Hoxa10 in canine uterus during early pregnancy and its regulation under different conditions by in situ hybridization. Hoxa10 mRNA was mainly localized in glandular epithelium and myometrium in canine uterus. There was a low level of Hoxa10 expression in the glandular epithelium on days 6, 12 and 17 of pregnancy. On day 20 of pregnancy when embryo implanted, Hoxa10 mRNA was highly expressed in the glandular epithelium surrounding the embryo, but not in the luminal epithelium. The expression of Hoxa10 mRNA gradually declined from day 23 and reached a low level on day 28. In the myometrium, a low level of Hoxa10 mRNA signal was seen on days 6, 12 and 17 of pregnancy and reached a high level on day 20 of pregnancy. During the estrous cycle, a high level of Hoxa10 mRNA expression was seen in the estrous uterus. Either estrogen or progesterone significantly induced the expression of Hoxa10 mRNA in the ovariectomized canine uterus. These results suggest that Hoxa10 expression is closely related to canine embryo implantation and upregulated by estrogen and progesterone. 相似文献
14.
Uteroferrin,also known as type 5 tartrate resistant acid phosphatase ( ACP5 ) or TRAP,is an iron-containing glycoprotein secreted by uterine gland epithelium (GE) in response to progesterone and transported across the placental areoalae into the fetal circulation and allantoic fluid to deliver iron and to stimulate hematopoeisis in pigs.This study determined if ACP5 was expressed in the ovine uterus in response to pregnancy,progesterone,interferon tau,placental lactogen,and placental growth hormone.ACP5 protein was present in uterine GE of cycfic and early pregnant ewes,particularly between days 18 and 120 of pregnancy.ACP5 mRNA was expressed in uterine GE of cyclic and pregnant ewes in the same temporal and cell-specific manner.ACP5 was present in secretions from uterine glands,i.e.,uterine milk,and aUantoic fluid from days 40 to 80 of pregnancy,and in uterine flushings from cyclic and early pregnant ewes.Progesterone induced expression of ACP5 mRNA and intrauterine infusion of recombinant ovine interferon tau further stimulated ACP5 expression in uterine GE of ewes,but intrauterine injections of ovine placental lactogen and ovine growth hormone had no effect on ACP5 expression in uterine GE.These results indicate that ACP5 is:1 ) expressed only in GE in response to progesterone ;2 ) secreted into the uterine lumen and transported into the conceptus via plaeental areolae during pregnancy;and 3) present in secretions from uterine GE and in allantoic fluid.The roles of ACP5 in the ovine uterus may include transport of iron across the placenta and stimulation of hematopoiesis. 相似文献
15.
Kubota K Yamauchi N Matsumoto K Watanabe R Oozono S Aramaki S Wood C Soh T Hattori MA 《The Journal of reproduction and development》2008,54(5):340-345
Hedgehog (Hh) plays a pivotal role in various tissues during embryonic development, tissue homeostasis and tumorigenesis. In mammals, Hh exists in three homologs: Desert hedgehog (Dhh), Indian hedgehog (Ihh) and Sonic hedgehog (Shh). In this study, we cloned full-length cDNAs encoding Dhh and Ihh from the rat uterus. Their amino acid sequences have a high homology with those of the mouse and human. In addition, the changes of Hh gene expression in the rat uterus during early pregnancy were analyzed. The results showed that all three hedgehog mRNAs were detected in the rat uterus at the proestrus stage and during early pregnancy (1.5, 3.5, 5.5 and 7.5 days post coitus: dpc). Ihh mRNA expression varied and peaked at 3.5 dpc in the luminal and glandular epithelium. Expression was decreased on 5.5 dpc with the exception of sustained expression in the glandular epithelium. Despite such Ihh variability, the expressions of Dhh and Shh mRNA remained unchanged. This indicated that Ihh was mainly expressed in the rat uterus during early pregnancy. Moreover, the Hh target gene (glioma-associated oncogene homolog 1; Gli1) was also highly expressed at 3.5 dpc in the epithelium and periepithelial stroma in a manner similar to the temporal pattern of Ihh expression. This suggests that Ihh signaling axis play a role in the rat uterus during early pregnancy. In summary, our results elucidate that Ihh is a predominant Hh protein in the rat uterus during early pregnancy and that other Hhs have the potential to be expressed. This observation will help to elucidate the basic molecular mechanism of rat uterus during early pregnancy. 相似文献
16.
《中国畜牧杂志》2019,(9)
膜联蛋白A8(Annexin A8,ANXA8)是一种磷脂结合蛋白,与炎症反应、癌症的发生以及血管生成有密切联系。本实验旨在利用实时荧光定量PCR、原位杂交与免疫组织化学的方法研究ANXA8 mRNA与蛋白在小鼠早期妊娠和人工蜕膜子宫中的表达。原位杂交结果表明:ANXA8 mRNA在小鼠早期妊娠第1～4天子宫腔上皮和腺上皮有微弱表达,ANXA8 mRNA在妊娠第5、6天的初级蜕膜区与第7、8天的次级蜕膜区表达,并随妊娠进行逐渐增强;人工蜕膜化模型中ANXA8 mRNA表达在蜕膜区。实时荧光定量PCR证明:ANXA8 mRNA的表达量在早期妊娠模型中的第7、8天显著提高,人工蜕膜侧子宫与对照侧相比也显著提高。免疫组织化学结果表明:ANXA8蛋白与ANXA8 mRNA表达规律相似。体外分离培养小鼠子宫基质细胞,并诱导蜕膜化,实时荧光定量PCR结果表明ANXA8随着基质细胞的蜕膜化表达升高。以上体内和体外实验表明,ANXA8在小鼠子宫中的表达具有着床相关特异性,ANXA8参与小鼠子宫蜕膜化过程。 相似文献
17.
B Guo B-C Han Z Tian T-Y Zhao L Zeng Z-M Yang Z-P Yue 《Reproduction in domestic animals》2009,44(5):779-782
Embryo implantation is critical for the successful establishment of pregnancy. Interleukin-11 (IL-11) is essential for adequate decidualization in the mouse and human via binding to the specific IL-11 receptor α (IL-11Rα). But the expression and regulation of IL-11 and IL-11Rα in the canine endometrium remain unknown. The aim of this study was to investigate the differential expression of IL-11Rα in canine uterus during early pregnancy and its regulation under different conditions by in situ hybridization. Interleukin-11Rα mRNA was mainly localized in glandular epithelium in canine uterus. There was a low level of IL-11Rα expression in the glandular epithelium on days 6, 12 and 17 of pregnancy. On day 20 of pregnancy when embryo implanted, IL-11Rα mRNA was highly expressed in the glandular epithelium surrounding the embryo, but not in the luminal epithelium and stroma. On day 23 of pregnancy, the expression of IL-11Rα mRNA maintained a constant level compared with the expression of day 20 and increased on day 28 of pregnancy. During the oestrous cycle, a high level of IL-11Rα mRNA expression was seen in the oestrous uterus. Progesterone slightly induced the expression of IL-11Rα mRNA in the ovariectomized canine uterus. These results suggest that IL-11Rα expression is closely related to canine implantation and up-regulated by progesterone. 相似文献
18.
M Słomczyńska M Duda M Burek K Knapczyk D Czaplicki M Koziorowski 《Reproduction in domestic animals》2008,43(1):35-41
The uterus is a well-known target of endocrine, paracrine and autocrine acting molecules among which steroid hormones (oestrogens, androgens and progesterone) are of special importance. The uterine tissues (endometrium and myometrium) undergo morphological and physiological changes which are associated with changes in expression of steroid hormone receptors. Androgen receptors (AR) that mediate the action of androgens have already been detected in porcine uteri during the oestrous cycle and early pregnancy. To evaluate the role of AR in uterine physiology, the presence of ARmRNA and AR protein localization in the porcine uterus from day 10 to day 90 of pregnancy and in the uterus from the foetus of day 90 postcoitum (p.c.) and from the neonatal 1-day-old piglet was studied. ARmRNA was detected in the porcine endometrium up to day 18 p.c., while AR protein was detectable in glandular epithelium and stromal cells as through day 90 of pregnancy. AR was also detected in the myometrium on all investigated days of pregnancy; however, on day 90, the immunostaining was present only in a limited number of cells. AR immunostaining was clearly demonstrated in the uterus of the female foetuses on day 90 as well as in the uterus of 1-day-old piglets. The physiological relevance of this finding needs further elucidation. 相似文献
19.