Lipopolysaccharide and cytokines modulate leukotriene (LT)B4 and LTC4 production by porcine endometrial endothelial cells          下载免费PDF全文

J Czarzasta  K Meller  A Andronowska  B Jana 《Reproduction in domestic animals》2018,53(1):101-109

Uterine inflammatory response is mediated by inflammatory mediators including eicosanoids and cytokines produced by immune and endometrial cells. Interactions between lipopolysaccharide (LPS) and cytokines, and leukotrienes (LTs) in endothelium, important for the host defence during the inflammation, are unknown. We studied the effect of LPS, tumour necrosis factor (TNF)‐α, interleukin (IL)‐1β, IL‐4 and IL‐10 on 5‐lipooxygenase (5‐LO), LTA₄ hydrolase (LTAH) and LTC₄ synthase (LTCS) mRNA and protein expression, LTB₄ and LTC₄ release from porcine endometrial endothelial cells, and cell viability. For 24 hr, cells were exposed to LPS (10 or 100 ng/ml of medium) and cytokines (each 1 or 10 ng/ml). 5‐LO mRNA/protein expression augmented after incubation with larger doses of LPS, TNF‐α, IL‐4 and IL‐10 and smaller dose of IL‐1β. Larger dose of TNF‐α, smaller doses of LPS and IL‐1β and both doses of IL‐10 increased LTAH mRNA/protein expression. LTAH protein content was up‐regulated by larger dose of LPS, but it was reduced in response to both doses of IL‐4. LTCS mRNA expression was elevated by larger doses of LPS, IL‐4 and IL‐10 or both doses of TNF‐α and IL‐1β. LTCS protein level increased after treatment with both doses of IL‐1β, IL‐4 and IL‐10, smaller dose of LPS and larger dose of TNF‐α. Both doses of LPS and larger doses of TNF‐α and IL‐10 increased LTB₄ release. LPS, IL‐1β and IL‐10 at smaller doses, or TNF‐α and IL‐4 at larger doses stimulated LTC₄ release. Smaller doses of TNF‐α and IL‐1β or both doses of IL‐4 enhanced the cell viability. This work provides new insight on the participation of LPS, TNF‐α, IL‐1β, IL‐4 and IL‐10 in LTB₄ and LTC₄ production/release from porcine endometrial endothelial cells, and the effect of above factors on these cells viability. The used cellular model gives the possibility to further establish the interactions between inflammatory mediators.  相似文献   

Of rodents and ruminants: a comparison of small noncoding RNA requirements in mouse and bovine reproduction     

Lauren G Chukrallah  Aditi Badrinath  Kelly Seltzer  Elizabeth M Snyder 《Journal of animal science》2021,99(3)

Ruminants are major producers of meat and milk, thus managing their reproductive potential is a key element in cost-effective, safe, and efficient food production. Of particular concern, defects in male germ cells and female germ cells may lead to significantly reduced live births relative to fertilization. However, the underlying molecular drivers of these defects are unclear. Small noncoding RNAs, such as piRNAs and miRNAs, are known to be important regulators of germ-cell physiology in mouse (the best-studied mammalian model organism) and emerging evidence suggests that this is also the case in a range of ruminant species, in particular bovine. Similarities exist between mouse and bovids, especially in the case of meiotic and postmeiotic male germ cells. However, fundamental differences in small RNA abundance and metabolism between these species have been observed in the female germ cell, differences that likely have profound impacts on their physiology. Further, parentally derived small noncoding RNAs are known to influence early embryos and significant species-specific differences in germ-cell born small noncoding RNAs have been observed. These findings demonstrate the mouse to be an imperfect model for understanding germ-cell small noncoding RNA biology in ruminants and highlight the need to increase research efforts in this underappreciated aspect of animal reproduction.  相似文献   

Identification of two glycerophosphodiester phosphodiesterase genes in maize leaf phosphorus remobilization     

Jingxin Wang  Wenbo Pan  Alexiy Nikiforov  William King  Wanting Hong  Weiwei Li  Yang Han  Jana Patton-Vogt  Jianbo Shen  Lingyun Cheng 《作物学报(英文版)》2021,(1):95-108

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Algicidal and cyanocidal effects of selected isoquinoline alkaloids     

Daniel Jančula  Jana Gregorová  Blahoslav Maršálek 《Aquaculture Research》2010,41(4):598-601

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Interaction of arbuscular mycorrhizal fungi and rhizobia: Effects on flax yield in spoil‐bank clay     

Jana Rydlová  David Püschel  Radka Sudová  Milan Gryndler  Olga Mikanová  Miroslav Vosátka 《植物养料与土壤学杂志》2011,174(1):128-134

This study focused on the application of native strains of arbuscular mycorrhizal fungi (AMF) and Sinorhizobium in effective crop production during reclamation of coal‐mine spoil banks. Two greenhouse experiments were conducted in spoil‐bank clay with a low dose of organic amendment to determine whether the microbial inoculation improves growth and utility qualities of two cultivars of Linum usitatissimum L. (oil and fiber flax). Inoculation with two native AMF isolates (Glomus mosseae, G. intraradices, and their mixture) significantly increased growth and shoot phosphorus (P) concentration of both flax cultivars. Inoculated fiber flax plants produced fivefold more fibers than the uninoculated ones. In oil flax, mycorrhizal inoculation significantly but quantitatively to a minor degree decreased the concentration of nonsaturated fatty acids in the seed oil. A mixture of five native Sinorhizobium sp. strains supported growth and P uptake of oil flax only in the absence of AMF. However, these beneficial effects of the bacteria were significantly lower as compared to AMF. No synergic action of Sinorhizobium strains and AMF was observed, and their interactions were often even antagonistic. Inoculation with AMF significantly decreased population density of Sinorhizobium in the soil. These results suggest that a careful selection of suitable bacterial strains is necessary to provide effective AMF combinations and maximize flax‐growth support.  相似文献   

Response of the pigeonpea-Rhizobium symbiosis to salinity stress: variation among Rhizobium strains in symbiotic ability     

G. V. Subbarao  C. Johansen  J. V. D. K. Kumar Rao  M. K. Jana 《Biology and Fertility of Soils》1990,9(1):49-53

Summary There were significant differences among pigeonpea [Cajanus cajan (L.) Millsp] Rhizobium sp. strains (IC 3506, IC 3484, IC 3195, and IC 3087) in their ability to nodulate and fix N₂ under saline conditions. Pigeonpea plants inoculated with IC 3087 and IC 3506 were less affected in growth by salinity levels of 6 and 8 dS m^-1 than plants inoculated with the other strains. For IC 3506, IC 3484, and IC 3195, there was a decrease in the number of nodules with increasing salinity, while the average nodule dry weight and the specific nitrogenase activity remained unaffected. However, in IC 3087, the number of nodules increased slightly with increasing salinity. Leaf-P concentrations increased with salinity in the inoculated plants irrespective of the Rhizobium sp. strain, and leaf-N concentrations decreased with increasing salinity in IC 3484 and IC 3195 only. Shoot-Na and-Cl levels were further increased in these salt-sensitive strains only at 8 dS m^-1. Therefore there may be scope for selecting pigeonpea Rhizobium sp. symbioses better adapted to saline conditions. The Rhizobium sp. strains best able to form effective symbioses at high salinity levels are not necessarily derived from saline soils.Submitted as JA No. 919 by the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)  相似文献   

Effects of clothianidin‐treated seed on the arthropod community in a mid‐Atlantic no‐till corn agroecosystem     

Heather H Disque  Kelly A Hamby  Aditi Dubey  Christopher Taylor  Galen P Dively 《Pest management science》2019,75(4):969-978

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Investigation on hydrostatic resistance and thermal performance of layered waterproof breathable fabrics     

Abdur Razzaque  Pavla Tesinova  Lubos Hes  Jana Salacova  Hafiz Affan Abid 《Fibers and Polymers》2017,18(10):1924-1930

Waterproof breathable layered fabrics allow water vapor passing through, but resist liquid water to pass. This ability of the fabrics to protect rain and snow water while allowing sweat vapor to evaporate from inside to outside atmosphere, leads them to be used as outdoor sportswear or protective clothing. The big challenge of enhanced hydrostatic resistance of these fabrics with proper breathability and thermal comfort has widened the research scope. This study presents an experimental investigation on hydrostatic resistance and thermal behavior of layered waterproof breathable fabrics. Six different types of hydrophobic and hydrophilic membrane laminated layered fabrics were evaluated by varying different fabric parameters in the experiment. Hydrostatic resistance and water vapor permeability of the laminated fabrics were measured by SDL ATLAS Hydrostatic Head Tester and PERMETEST respectively. Thermal properties were evaluated by ALAMBETA instrument. Moreover, FX-3300 air permeability tester was used to measure air permeability which represents the porosity of the fabrics and computer based See System software was used for water contact angle measurement on the outer fabric surface in order to determine the hydrophobic and hydrophilic properties. This experiment clearly discusses the influence of different fabric characteristics and parameters on hydrostatic resistance and thermal properties of the breathable laminated fabrics. The results show that fabric material composition, density, thickness, and hydrophobic and hydrophilic membranes have significant effects on hydrostatic resistance, breathability and thermal properties of different laminated fabrics.  相似文献   

Identification of genic SSRs in jute (Corchorus capsularis,Malvaceae) and development of markers for phenylpropanoid biosynthesis genes and regulatory genes          下载免费PDF全文

Pratik Satya  Avrajit Chakraborty  Sourav Jana  Snehalata Majumdar  Maya Karan  Debabrata Sarkar  Subhojit Datta  Jiban Mitra  Chandan S. Kar  Pran Gobinda Karmakar  Nagendra K. Singh 《Plant Breeding》2017,136(5):784-797

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Phosphorus rather than nitrogen enhances CO2 emissions in tropical forest soils: Evidence from a laboratory incubation study     

Dafeng Hui  Wesley Porter  Jana R. Phillips  Marcos P.M. Aidar  Steven J. Lebreux  Christopher W. Schadt  Melanie A. Mayes 《European Journal of Soil Science》2020,71(3):495-510

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