Finasteride-induced prostatic involution by apoptosis in dogs with benign prostatic hypertrophy     

Sirinarumitr K  Sirinarumitr T  Johnston SD  Sarkar DK  Kustritz MV 《American journal of veterinary research》2002,63(4):495-498

OBJECTIVE: To determine the effect of finasteride on programmed cell death (apoptosis) of prostatic cells during prostatic involution in dogs with benign prostatic hypertrophy (BPH). ANIMALS: 9 dogs with BPH. PROCEDURE: Dogs were randomly assigned to treatment or control groups. Treatment dogs (n = 5) were administered finasteride (0.1 to 0.5 mg/kg, PO, q 24 h) for 16 weeks, whereas the 4 control dogs were administered an inert compound. Prostatic cells from the prostatic fluid portion of the ejaculate of treatment and control dogs were obtained before and 1, 2, 3, 4, 8, and 16 weeks after initiation of treatment. Cells were concentrated by use of centrifugation. Prostatic cells were examined for indications of apoptosis by use of a terminal deoxyribonucleotidyl transferase-mediated deoxyuracil triphosphate nick-end labeling technique. After receiving the inert compound for 16 weeks, the 4 control dogs were administered finasteride for 16 weeks, and evaluations were repeated. RESULTS: Percentage of apoptotic prostatic cells in ejaculated prostatic fluid of treatment dogs increased significantly (from 9% before treatment to 33, 31, 26, and 27% after 1, 2, 3, and 8 weeks of treatment, respectively). There was no significant change in percentage of apoptotic prostatic cells in the ejaculated prostatic fluid of control dogs. CONCLUSIONS AND CLINICAL RELEVANCE: Finasteride-induced prostatic involution appears to be via apoptosis in dogs with BPH. Finasteride treatment of dogs with BPH causes prostatic involution by apoptosis rather than necrosis.  相似文献   

Effects of exercise intensity and duration on plasma beta-endorphin concentrations in horses     

Mehl ML  Schott HC  Sarkar DK  Bayly WM 《American journal of veterinary research》2000,61(8):969-973

OBJECTIVE: To determine the relationship between plasma beta-endorphin (EN) concentrations and exercise intensity and duration in horses. ANIMALS: 8 mares with a mean age of 6 years (range, 3 to 13 years) and mean body weight of 450 kg. PROCEDURE: Horses were exercised for 20 minutes at 60% of maximal oxygen consumption (VO2max) and to fatigue at 95% V02max. Plasma EN concentrations were determined before exercise, after a 10-minute warmup period, after 5, 10, 15, and 20 minutes at 60% VO2max or at the point of fatigue (95% VO2max), and at regular intervals after exercise. Glucose concentrations were determined at the same times EN concentrations were measured. Plasma lactate concentration was measured 5 minutes after exercise. RESULTS: Maximum EN values were recorded 0 to 45 minutes after horses completed each test. Significant time and intensity effects on EN concentrations were detected. Concentrations were significantly higher following exercise at 95% VO2max, compared with those after 20 minutes of exercise at 60% VO2max (605.2 +/- 140.6 vs 312.3 +/- 53.1 pg/ml). Plasma EN concentration was not related to lactate concentration and was significantly but weakly correlated with glucose concentration for exercise at both intensities (r = 0.21 and 0.30 for 60 and 95% VO2max, respectively). CONCLUSIONS AND CLINICAL RELEVANCE: A critical exercise threshold exists for EN concentration in horses, which is 60% VO2max or less and is related to exercise intensity and duration. Even under conditions of controlled exercise there may be considerable differences in EN concentrations between horses. This makes the value of comparing horses on the basis of their EN concentration questionable.  相似文献   

Subjective and objective measurements of postoperative pain in cats     

Cambridge AJ  Tobias KM  Newberry RC  Sarkar DK 《Journal of the American Veterinary Medical Association》2000,217(5):685-690

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Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves     

Brajesh C. Varshney  N.M. Ponnanna  Pranati A. Sarkar  Pragna Rehman  Jigar H. Shah 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):57-64

Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carried out by sodium deoxycholate treatment and/or ion-exchange chromatography. Monoclonal antibodies to purified K99 antigen were produced by the hybridoma technique, and a specific clone, NEK99-5.6.12, was selected for propagation in tissue culture. The antibodies, thus obtained, were affinity-purified, characterized and coated onto Giemsa-stained Cowan-I strain of Staphylococcus aureus (S. aureus). The antibody-coated S. aureus were used in a co-agglutination test to detect K99+ E. coli isolated from feces of diarrheic calves. The specificity of the test was validated against reference monoclonal antibodies used in co-agglutination tests, as well as in ELISA. Specificity of the monoclonal antibodies was also tested against various Gram negative bacteria. The developed antibodies specifically detected purified K99 antigen in immunoblots, as well as K99+ E. coli in ELISA and co-agglutination tests. The co-agglutination test was specific and convenient for large-scale screening of K99+ E. coli isolates.  相似文献   

Überleben einerXanthomonas campestris pv.pelargonii-Mutante in einjähriger Komposterde     

Dr. M. Wölk  Prof. Dr. S. Sarkar 《Journal of pest science》1994,67(4):69-71

Zusammenfassung Das Überleben vonXanthomonas campestris pv.pelargonii wurde anhand einer Mutante in einjährigem Kompost untersucht. In der Versuchsvariante 1, bei der die Bakterien mit Komposterde vermischt wurden, konnte die Mutante Xc3b nach 15 Monaten nicht mehr nachgewiesen werden. Unter diesen Bedingungen war es den Bakterien möglich, 14 Monate im Kompost zu überleben. In der Versuchsvariante 2, bei der Komposterde mit Bakterien und zusätzlich noch mit infizierten Pelargonienstielen eingebracht wurden, konnten dieXanthomonas-Mutanten noch nach 26 Monaten nachgewiesen werden.

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Survival of a mutant ofXanthomonas campestris pv.pelargonii in one year old compost

Survival of phytopathogenicXanthomonas campestris pv.perlargonii in a one year old compost was investigated by use of a mutant. In the variant 1, in which the compost soil was mixed with the bacteria, the mutant Xc-3b could not be detected after 15 months. Under these conditions the bacteria were able to survive for 14 months in the compost. In the variant 2, in which the compost soil was mixed with bacteria andXanthomonas-infected geranium stems, it could be found even after 26 months.

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Mit 2 Abbildungen  相似文献   

Antagonismusin vitro vonBacillus spp. gegenüberRhizoctonia solani undPythium spp.     

Dr. M. Wölk  Prof. S. Sarkar 《Journal of pest science》1993,66(7):121-125

Zusammenfassung Es konnten 132Bacillus-Stämme isoliert und gegenüber7 Rhizoctonia-solani- und 6Pythium-Stämmen auf Antagonismusin vitro untersucht werden. Die gefundenen Antagonisten hemmten die Pilzisolate unterschiedlich stark, so daß Gemische von Antagonisten für die Praxis zu empfehlen sind.

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Antagonismin vitro ofBacillus spp. againstRhizoctonia solani andPythium spp.

There were 132Bacillus-strains isolated and tested for antagonism against 7 strains ofRhizoctonia solani and 6 strains ofPythium spp. The isolated antagonists didn't show a uniform effect against the tested strains ofRhizoctonia solani andPythium spp. For an application in practice it will be better to use a mixture of antagonists.

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Mit 4 Abbildungen und einer Tabelle  相似文献   

Isolation und Pathogenität vonRhizoctonia solani Kühn an Gurken und Buschbohnen     

Dr. M. Wölk  Prof. Dr. S. Sarkar 《Journal of pest science》1994,67(2):34-39

Zusammenfassung Es wurden 79Rhizoctonia solani-Isolate von verschiedenen Orten in Baden-Württemberg isoliert. Es konnten Isolate gefunden werden, die gegenüber Gurken und Bohnen pathogen waren. Bei Bohnen kann das Sproßgewicht als leicht zu erfassender Parameter für die Quantifizierung der Schadwirkung benutzt werden. Eine trockene Lagerung von mitRhizoctonia solani durchwachsenen Haferkörnern erwies sich als besonders geeignet für eine Stammkulturhaltung.

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Isolation and pathogenicity ofRhizoctonia solani to cucumber and bean

79Rhizoctonia solani strains were isolated from different locations in Baden-Württemberg. Some of these strains were pathogenic against cucumber and bean. The sprout-weight of bean can be used as an easy-to-measure parameter for quantification of the damage caused by teh fungus. The dry storage of oat seeds, previously inoculated withRhizoctonia solani, has been shown to be the best method for long-term storage.

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Mit einer Abbildung und 6 Tabellen  相似文献   

Evaluation of the nutritive value of Leucaena leucocephala leaf meal, inoculated with fish intestinal bacteria Bacillus subtilis and Bacillus circulans in formulated diets for rohu, Labeo rohita (Hamilton) fingerlings     

A Bairagi  K Sarkar Ghosh  S K Sen  & A K Ray 《Aquaculture Research》2004,35(5):436-446

Nine isonitrogenous (35% crude protein approximately) and isocaloric (18.37 kJ g^?1) experimental diets (RLL20–BCFL40) were formulated with either raw or treated (inoculated with fish intestinal bacteria) Leucaena leucocephala leaf meal at 20%, 30% and 40% levels replacing other ingredients partially from a fish meal based reference diet (RD). Two specific strains of fish intestinal bacteria, Bacillus subtilis (isolated from Cyprinus carpio) and B. circulans (isolated from Oreochromis mossambicus) having extracellular cellulolytic and amylolytic activities, were used to inoculate Leucaena leaf meal for 15 days at 37°C. The crude fibre, cellulose and hemicellulose contents and the antinutritional factors, tannin, phytic acid and mimosine in the leaf meal decreased due to inoculation. However, free amino acids and fatty acids increased in the treated leaf meal. The response of rohu, Labeo rohita, fingerlings fed the experimental diets for 80 days was compared with fish fed a RD. Both the inclusion level and type of Leucaena leaf meal in diets significantly affected the growth performance of rohu. Fish fed diets containing inoculated Leucaena leaf meal performed better in comparison with those with the RD. On the basis of growth response, feed conversion ratio, protein efficiency ratio and apparent net protein utilization, diet formulated with 30%Leucaena leaf meal inoculated with B. circulans resulted in the best performance of rohu fingerlings followed by diet with 40%B. subtilis inoculated Leucaena leaf meal. The apparent protein digestibility (APD) was better in fish fed diets containing B. circulans inoculated leaf meal. An increasing level of raw Leucaena leaf meal was associated with a decrease in the carcass protein content of rohu fingerlings. The activity of α‐amylase increased with the increasing level of treated leaf meal in diets. Cellulase activity increased with increasing level of inclusion of raw leaf meal, and was comparatively lower in fish fed diets with treated leaf meal. Activities of protease and lipase were higher in fish fed the RD. The results showed that it is possible to incorporate Leucaena leaf meal inoculated with enzyme‐producing fish intestinal bacteria in carp diets up to 40% level of inclusion.  相似文献   

Particulate phase polycyclic aromatic hydrocarbons in the ambient atmosphere of a protected and ecologically sensitive area in a tropical megacity     

Darpa Saurav Jyethi  P.S. Khillare  Sayantan Sarkar 《Urban Forestry & Urban Greening》2014,13(4):854-860

This study reports, for the first time, the profiles and source analysis of 16 US EPA priority polycyclic aromatic hydrocarbons (PAHs) associated with PM₁₀ (particulate matter with aerodynamic diameter ≤10 μm) at a protected and ecologically sensitive area – the Yamuna Biodiversity Park – located in the megacity Delhi, India. Weekly PM₁₀ sampling was carried out at this location for 1 year (2009–2010) and the annual mean PM₁₀ level was found to be ～9 times the World Health Organization limit. Seasonal variation of PAHs (range 37.2–74.0 ng m^?3) was significant with winter values being 72% and 68% higher than summer and monsoon respectively. Principal component analysis coupled with multiple linear regression identified diesel, natural gas and lubricating oil combustion (49.5%), wood combustion (25.4%), gasoline (15.5%) and coal combustion (9.6%) sources for the observed PAHs. Heavy traffic on the national highway and arterial roads and domestic emissions from suburban households in the vicinity of the park appeared to have significantly affected its air quality. A substantial portion (～55%) of the aerosol PAH load was comprised of carcinogenic species, which yielded a considerably high lifetime inhalation cancer risk estimate (8.7E?04). If considered as a conservative lower-bound estimate, this risk translates into ～211 excess cancer cases for lifetime inhalation exposure to the observed PAH concentrations in Delhi.  相似文献   

Pattern of physicochemical changes in the water during hatching of Indian carp eggs in a Chinese hatchery system     

B.B. Jana  G. Sarkar  G. Kundu 《Aquaculture (Amsterdam, Netherlands)》1985,47(1):89-96

Physicochemical changes in the water were monitored at different hours of embryonic and larval development of Indian carp (Cirrhinus mrigala) eggs in a Chinese hatchery system with 75–85% hatching success. At a water temperature of 29.3°C, mass hatching of the embryos started at about 11–12 h after fertilization and was completed within 4 h. The amount of dissolved oxygen and chemical oxygen demand ranged between 4.8 and 9.63 mg l^?1 and 16.5 and 185.0 mg l^?1, respectively. A marked rise in the concentration of bicarbonate and total hardness of the water after egg transfer into hatching tanks was perhaps due to liberation of Ca²⁺ and Mg²⁺ from the egg mass. A sharp decline in phosphate content during hatching was associated with the building of bones, while the ammonia level was greatly increased due to a high rate of nitrogenous excretion during hatching.  相似文献