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Abstract.— Quantitative changes in the protein, lipid and carbohydrate were studied in the early larval stages of developing freshwater prawn Macrobrachium rosenbergii reared under fed and starved conditions to understand the relative importance of these nutrients in energy metabolism of the growing larvae. Larvae obtained from several females were stocked into three 250-L tanks at a density of 30 larvae/L. The feeding regime consisted of newly hatched Artemia nauplii. Protein was always the major organic constituent followed by lipids and then carbohydrates of both fed and starved larvae. Protein levels of both fed and starved larvae increased during development, suggesting an important role in morphogenesis. The decline of lipid during the larval growth that was more rapid for starved larvae, suggests a probable utilization of lipid as the major metabolic source of energy. Carbohydrates formed less than 5 and 2.4% of the larval dry weight of fed and starved larvae, respectively, suggesting their limited role in larval metabolism.  相似文献   
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‘Gold standard’ OIE reference PCR assay was utilized to detect the presence of infectious spleen and kidney necrosis virus (ISKNV) in freshwater ornamental fish from Malaysia. From total of 210 ornamental fish samples representing 14 species, ISKNV was detected in 36 samples representing 5 fish species. All positive cases did not show any clinical signs of ISKNV. Three restriction enzymes analyses showed that the fish were infected by identical strains of the same virus species within Megalocytivirus genus. Major capsid protein (MCP) genes of 10 ISKNV strains were sequenced and compared with 9 other reference nucleotide sequences acquired from GenBank. Sequence analysis of MCP gene showed that all strains detected in this study were closely related to the reference ISKNV with nucleotide sequence identity that was ranging from 99.8% to 100%. In addition, phylogenetic analysis of MCP gene revealed that viruses from genus Megalocytivirus can be divided into three genotypes: genotype 1 include reference ISKNV and all other strains that were detected in this study, genotype 2 include viruses closely related to red sea bream iridovirus (RSIV), and genotype 3 include viruses closely related turbot reddish body iridovirus (TRBIV).  相似文献   
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Abstract.— Amino acid profiles of freshwater prawn Macrobrachium rosenbergii were determined during early larval stages (I-IX) to provide some baseline biochemical information of the growing larvae. The larvae obtained from several females were stocked into three 250-L tanks at a density of 30 larvae/L. The feeding regime consisted of newly hatched Artemia nauplii and egg custard containing 5% cod liver oil. For each developmental stage, larval samples from each experimental tank were pooled, freeze dried and after acid hydrolysis, the quantification of amino acids was done by a reverse phase high performance liquid chromatography ( N = 2). The highest represented of the total amino acids were for glutamic acid and phenylalanine (with cystine) with ranges from 13.4–16.6 and 9.7–11.5%, respectively, whereas tryptophan (1.4–1.6%). methionine (1.4–2.7%) and histidine (2.9–4.2%) were relatively lower. The levels of the following essential amino acids did not significantly change during larval development: arginine, leucine, phenylalanine (plus cystine), threonine, tryptophan and valine. Despite statistically significant changes that were observed in levels of some amino acids, amino acid composition appeared to be relatively unchanged during the larval growth. The results may suggest that the amino acid requirements of the freshwater prawn is relatively constant during larval life and can be satistied by a suitable protein source that resembles the larval amino acid profile.  相似文献   
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Journal of Plant Diseases and Protection - Cowpea mosaic virus is among the most economically important viruses infecting cowpea. Incidence of infection was assessed in two governorates in Egypt,...  相似文献   
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[目的]研究土壤捕食性螨类群落结构及其多样性特征,为开发利用及保护乌鲁木齐地区农林害虫捕食性天敌资源提供科学依据.[方法]采用塔氏( Tullgren)分离法和群落多样性分析方法对该区不同生境土壤捕食性螨类资源的群落结构、多样性及其季节动态变化进行比较分析.[结果]乌鲁木齐市不同生境土壤农林害虫捕食性螨类3643只,分别隶属于2目21科38属.其中前气门目螨类有5科11属,中气门目螨类有16科27属,其中优势类群为美绥螨属Ameroseius、囊螨属Asca、真长须螨属Eustigmaeus和表刻螨属Epicrius 等四类,占总捕获量的61.57;.常见类群为背刻螨属Epicriopsis、毛绥螨属Lasioseius、吸螨属Bdella、肉食螨属Cheyletus、寄螨属Parasitus、真派盾螨属Euparholaspulus、尾足螨属Uropoda、维螨属Veigaia、厉螨属Hypoaspis、土厉螨Ololaelaps等10类,占总捕获量的30.47;,其余14类为稀有类群,仅占总捕获量的7.96;.在不同生境土壤捕食性螨类个体数和类群数之间差异均为显著(P<0.05),其中自然榆林最高,菜地最低,个体数顺序为自然榆林>植物园>菜地>防护林>居民点>草地.群落类群数及个体数的垂直分布均具有表聚性,不同生境及不同土层间差异均为极显著(P<0.01),个体数和类群数在不同季节之间差异显著(P<0.05),其顺序为夏季>秋季>冬季>春季.群落相似性系数在不同生境样地之间基本上属于中等不相似(0.25≤q<0.50).群落多样性指标在不同生境间差异显著(P<0.05),其中Shannon - Wiener多样性指数(H)顺序为自然榆林>植物园>草地>菜地>居民点>防护林.Margalef丰富度指数(M)顺序为自然榆林>草地>植物园>防护林>居民点>菜地.[结论]乌鲁木齐地区土壤捕食性螨类资源相当丰富,因此,为了加强农林害虫的生物控制、提高绿色农产品产量,亟待进一步加强此类资源的保护利用研究.  相似文献   
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The hydrolysis kinetics of iprodione in alkaline solutions of pH 8.3 to 12 at 25°C have been determined by ultraviolet spectrophotometry. Under these conditions, iprodione leads quantitatively and irreversibly to N-(3,5-dichloroanilinocarbonyl)-N-(isopropylaminocarbonyl)glycine. The reaction is not subject to a general basic catalysis and the rate law takes the form Kobs = KOH- [OH?1]. The activation entropy of -77 J mol?1deg?1, the value of the kinetic solvent isotope effect kOH?/kOD? of 0.79 and the value of 0.60 for the Hammett parameter σ, obtained for the hydrolysis of a series of 3-aryl-N-isopropyl-2,4-dioxoimidazolidine-1-carboxamides are all in agreement with the rate-determining attack by the hydroxyl ion on the carbonyl in the 4-position of the hydantoin ring of the fungicide.  相似文献   
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After the civil war and the Hurricane-Mitch disaster, cattlemen in Nicaragua were forced to transport their cattle from lowland areas to higher, dryer areas of the country. These areas are natural ecological niches for the cattle grub Dermatobia hominis (L. Jr.) (Diptera: Cuterebridae). To determine the importance of this infestation, the Agricultural and Livestock-Forestry Ministry selected a central area of Nicaragua to run a pioneer survey program to acquire information about hosts involved, number of cases, treatments applied and general knowledge of 42 farmers about the life cycle of the parasite. The subjects were either farm owners or farm managers. Ninety-five percentage of the farms indicated cases of D. hominis infestation in their animals, with cattle being the most affected host (100% of the affected farms). There was poor understanding of the D. hominis life cycle, vectors and control methods. A misuse of insecticides for the treatment of larval infestation by D. hominis was indicated.  相似文献   
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