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1.
D H Du Plessis 《The Onderstepoort journal of veterinary research》1992,59(2):119-122
The serological reactivity in indirect ELISA of five different bluetongue virus (BTV) serotypes (4, 10, 15, 16 & 20) was compared using polyclonal antisera raised against virus particles and an outer structural protein, VP2. Rabbit and sheep antisera against BTV-10 produced higher ELISA values with their homologous antigens than with heterologous serotypes. A hyperimmune rabbit serum specific for virus particles was able to distinguish heterologous serotypes from each other, but a sheep serum from an infected animal was not. An antiserum directed against VP2, the protein responsible for serotype specificity in neutralization tests, was not serotype-specific in ELISA and cross-reacted with other serotypes. The discriminatory ability of a BTV-4 antiserum was improved by cross-absorption with heterologous antigens. This greatly reduced the ELISA signals with heterologous serotypes and produced an antiserum that was effectively serotype-specific. 相似文献
2.
The heterogenicity of Cowdria ruminantium stocks: cross-immunity and serology in sheep and pathogenicity to mice 总被引:2,自引:0,他引:2
J L Du Plessis L Van Gas J A Olivier J D Bezuidenhout 《The Onderstepoort journal of veterinary research》1989,56(3):195-201
Ten stocks of Cowdria ruminantium (Ball 3, Breed, Comoro, Germishuys, Kümm, Kwanyanga, Mali, Mara, Nonile and Welgevonden) were compared from a cross-immunity, serological and mouse pathogenicity point of view. They were found to differ in varying degrees. Except for the Ball 3, Comoro and Germishuys stocks that were similar but not identical, there was no pattern in the antigenic diversity of the 10 stocks. The Welgevonden stock emerged as the stock that elicits an immunity against most of the South African stocks. The inability of the reference Ball 3 stock to protect sheep against no fewer than 6 other stocks questions the advisability of retaining this stock as the vaccine stock. The antigenic diversity of the 10 stocks could not be correlated with the antibody levels detected with the indirect fluorescent antibody test, since the sera against all 10 stocks reacted positively to the Kümm stock antigen and the variation in titres was not stock-related. 相似文献
3.
J L Du Plessis C Gray M F Van Strijp 《The Onderstepoort journal of veterinary research》1992,59(4):337-338
A 3-fold increase in the numbers of Lyt-2+ T cells in the circulating blood of mice infected and re-infected with the Welgevonden stock of Cowdria ruminantium, as determined by flow cytometry, is supportive evidence that immunity in heartwater is cell-mediated. The rise in Lyt-2+ cells only after re-infection of the mice is further evidence that the development of immunity in heartwater is dependent on the unhindered and adequate replication of C. ruminantium. 相似文献
4.
Globally, amphibians are among the least appreciated vertebrates and are often negatively perceived by the general public. Such attitudes are particularly prevalent in South Africa, where fear, superstitions and myths associated with frogs are pervasive in some cultures. These attitudes could have harmful consequences both for the animals concerned and conservation efforts. This study aimed to investigate attitudes to frogs throughout South Africa across multiple age and ethnic groups. We used a multi-language survey in both hard-copy and online versions. Respondents (n = 2 295) comprised both genders across six age groups and five ethnic groups. A functionalist approach using structural equation modelling was used to assess how liking of frogs and cultural beliefs varied with socio-demographic factors. Attitudes varied significantly between groups, with males more likely to like frogs than females and age and level of education also significantly linked to liking. The influence of cultural beliefs and lack of knowledge also directly influenced negative attitudes towards frogs. More than 60% of respondents expressed an interest in learning more about frogs, indicating an opportunity for improving attitudes through educational outreach. The findings of this study can be used to inform current efforts to protect amphibians in South Africa through social interventions. 相似文献
5.
Spirocerca lupi is a nematode mainly affecting dogs but has been found in other animals, particularly carnivores. Anatomical areas of typical and aberrant migration vary. This report describes four cases of Spirocerca lupi causing neurological symptoms, similar to thoracolumbar disc syndrome, as a result of aberrant migration of the nematode into the spinal canal. In two of the cases, the nematode could be demonstrated intraoperatively. The third was discovered on post-mortem examination, while the fourth case displayed compelling evidence of Spirocerca lupi involvement. Surgical removal of the Spirocerca lupi nematode would be the treatment of choice in cases of spinal migration, with therapeutic and preventive treatment with doramectin instituted to treat remote sites and prevent re-infection. In areas endemic for spirocercosis, Spirocerca lupi should be considered as an important differential diagnosis in cases that are presented with clinical signs suggestive of a spinal cord lesion. 相似文献
6.
7.
The preparation of the antigen, details of the reagents, the titration of the antispecies conjugates and the execution of the indirect fluorescent antibody test are described. The sensitivity and specificity of the test and its applicability to the detection of antibodies to Cowdria ruminantium are recorded. The test is both highly specific and sensitive and can be applied to a wide range of studies on heartwater, including epidemiology, determination of the C. ruminantium infection rate of Amblyomma ticks and the evaluation of immunization against heartwater. The test can also be used to detect antibodies to the heartwater agent in the sera of game. 相似文献
8.
9.
M.A. du Plessis 《African Zoology》2013,48(3):275-276
The mean group size of kudus in an agricultural area was 3,9 and noticeably affected by hunting. A low male: female sex ratio was also recorded, but did not influence fecundity negatively. 相似文献
10.
A. J. du Toit 《Southern Forests》2013,75(1):27-38
This article deals with the results of time studies of pruning P. radiata from 22 ft. to 35 ft. with pole saws. The influence on pruning time of mean diameter and number of branches removed in the pruning operation, number of cones removed and time of day is investigated and a multiple regression equation, expressing the relationship between pruning time and these variables, is computed. A taper table is prepared and used to investigate the effect of pruning on the formation of knot-free timber. The economics of high-pruning is discussed. 相似文献