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排序方式: 共有519条查询结果,搜索用时 46 毫秒
1.
Kano R. Kubota A. Nakamura Y. Watanabe S. Hasegawa A. 《Veterinary research communications》2001,25(8):615-622
Using cDNA from a CRFK cell line as a template, PCR amplification was performed with the Ub1S and poly(dT) primers to isolate feline ubiquitin genes. Sequencing of the 495 bp PCR fragment revealed that the putative amino acids induced by this fragment gave a fusion protein consisting of a ubiquitin polypeptide (76 amino acids) and an extension protein of ribosomal proteins L40 (52 amino acids). The putative amino acid sequence of ubiquitin was identical to those of humans, rats and pigs.The recombinant glutathione S-transferase (GST)–feline ubiquitin fusion proteins were produced in Escherichia coli and purified. The fusion proteins had a molecular weight of about 42 kDa and were detected by immunoblot assay with rabbit anti-ubiquitin antiserum.The mRNAs from heat-shocked and non-heat-shocked cells were subjected to RT-PCR (Ub1S and poly(dT) primers) analysis. The molecular weights of the ubiquitinated proteins in heat-shocked CFRK cells were between 18 kDa and 24 kDa by immunoblot assay.These results suggested that there were more ubiquinated proteins in the heat-shocked CRFK cells than in the pre-heat-shocked cells. 相似文献
2.
Yuzo Kurokawa Miki Okita Hirokazu Kubota Yoshimasa Tsumiyama Ichiro Chikamatsu Akiyoshi Tanaka Taketo Obitsu Kensuke Kawamura 《Animal Science Journal》2021,92(1):e13591
Farm managers' decision to cull dairy cows is based on the cows' milk production, history of disorder(s), and reproductive performance, each of which affects dairy cows' lifetime (herd life and productive lifespan). We investigated the relationships among the incidence of clinical mastitis (CM), the reproductive performance, and the culling rate. We also assessed the effects of these relationships on the lifetimes of dairy cows, using the records made before and after the introduction of an automatic milking system (AMS) at Hiroshima University Farm. Milk yield, CM incidence density, and culling rate of dairy cows increased after the AMS introduction. The CM incidence was associated with an elongation of the calving interval in cows with the same parity. CM in the 1st parity might have caused the reductions of the cows' lifetime and their parity at culling. A higher age at first calving (AFC) was associated with an increase in culling rate but did not lead to a significant decrease in lifetime. Investigations of the factors mediating CM in the 1st parity or AFC with CM incidence or culling rate in the later stages might contribute to the control of lifetime of dairy cows. 相似文献
3.
Kazuhiro Shiozaki Momo Shiozaki Junko Masuda Akiko Yamauchi Shuichi Ohwada Toshiki Nakano Toshiyasu Yamaguchi Tadao Saito Koji Muramoto Minoru Sato 《Fisheries Science》2010,76(5):865-872
Angiotensin-I-converting enzyme (ACE) plays a crucial role in the crisis of hypertension. Some peptides that originate from
protease hydrolysates are known to suppress ACE activity in vitro and in vivo. Here, we investigated whether trypsin hydrolysate
of oyster Crassostrea gigas showed hypotensive activity and ACE inhibition. The hydrolysate significantly suppressed systolic blood pressure and ACE
activity in spontaneously hypertensive rats following a one-shot oral administration and a long-term feeding experiment lasting
9 weeks. Each hydrolysate from oyster tissue showed ACE inhibitory activity, indicating the hypotensive effect was due to
synergism. One potent ACE inhibitory peptide, Asp-Leu-Thr-Asp-Tyr, was identified from the hydrolysate of the striate muscle,
and the peptide exhibited hypotensive activity in vivo. Protease digestion analysis suggested that Asp-Tyr could be the real
effector of this penta-peptide in vivo. 相似文献
4.
Rhea Joy Carton-Kawagoshi Bessie Joy Elle Valeriano Corre Jr. Shuichi Satoh Masahiro Notoya Daisuke Fujita 《Aquaculture International》2014,22(2):573-584
This study tested the feasibility of a low-cost seaweed biofiltration system for pond-based aquaculture through an indoor-integrated fish-seaweed culture experiment using weekly nutrient supply regime and different seaweed stocking densities. The culture experiment was conducted in glass aquaria that were stocked with Gracilariopsis bailiniae at 3 densities (low = 0.5 kg m?2, middle = 2 kg m?2 and high = 3.5 kg m?2) and provided either with effluents from intensive milkfish (Chanos chanos) culture or with effluent-free seawater (control) as nutrient source. Stocking density was used as a factor in optimizing nutrient availability for growth and nutrient removal under such low water exchange conditions. Our results showed that G. bailiniae cultivated in milkfish effluents had higher growth, biomass and nitrogen yields than those cultivated in effluent-free seawater. Among the different stocking densities tested, highest growth rate (1.03 % day?1) was obtained in the middle density. Increasing biomass and nitrogen yields were also obtained at this density until the end of the culture period. Poorer growth rates at low and high stocking densities were attributed to light limitation from phytoplankton and self-shading, respectively. Due to seaweed treatment, average outflow concentration of NH4 + was reduced from half of its pretreated level. This study showed that a weekly effluent supply at 2 kg m?2 seaweed stocking density can sustainably support the growth of G. bailiniae as long as the dissolved nutrients are present at high levels. 相似文献
5.
Expression of HSP70 in response to heat-shock and its cDNA cloning from Mediterranean blue mussel 总被引:1,自引:0,他引:1
Haruhiko?ToyoharaEmail author Masatomi?Hosoi Isao?Hayashi Satoshi?Kubota Hisashi?Hashimoto Yoshihiro?Yokoyama 《Fisheries Science》2005,71(2):327-332
Expression of HSP70 in response to heat-shock was investigated at the protein and mRNA levels in Mediterranean blue mussel.
Western and Northern blot analyses revealed that HSP70 was expressed following heat-shock in the mantle at both protein and
mRNA levels, suggesting that gene expression of HSP70 is implicated in the cellular response to heat-shock stress in mussel.
It was then attempted to clone HSP70 cDNA in order to determine the primary structure of mussel HSP70. As a result, two full-length
cDNA encoding HSP70 were isolated from a cDNA library prepared from the heat-shocked mantle. The isolated cDNA consist of
single open reading frames of 2067 bp and 1911 bp which encode proteins of 689 amino acids and 637 amino acids, respectively.
Both HSP70 cDNA encode an ATPase do main, and a substrate-binding do main in addition to a Glu-Glu-Val-Asp (EEVD) peptide
motif that is specific for cytosolic HSP70. These findings suggest that the cDNA clones obtained in the present study encode
cytosolic HSP70. 相似文献
6.
Md. Shah Alam Sarker Shuichi Satoh Keisuke Kamata Yutaka Haga Yoshihiro Yamamoto 《Aquaculture Research》2012,43(4):538-545
A feeding experiment was conducted to investigate the effect of organic acids and/or lipid supplementation on growth, utilization and environmental loading of nitrogen (N) and phosphorus (P) in juvenile yellowtail fed fishmeal (FM) and plant protein (PP) diets. Six diets as FM (FM‐based), FM+P (FM with inorganic P), FM+L (FM with lipid), PP+CA (PP with citric acid), PP+L+CA (PP with lipid and citric acid) and PP+L+FA (PP with formic acid) were formulated. Yellowtails were fed each of the diets in duplicate groups; once a day, 6 days a week to near satiation at water temperature 19.0–25.0 °C for 16 weeks. Fishmeal with inorganic P gave the best growth while PP+L+FA the lowest. However, growth increased in PP+CA and PP+L+CA. Addition of lipid significantly increased N and P retention resulting in significant reduction in N and P excretion. Citric acid and FA supplementation to PP diets also increased retention of P; hence, its excretion was lowered. Thus, CA, FA and lipid in juvenile yellowtail diets can help to partially replace FM with PP sources and reduce inorganic P use to minimize environmental loading from aquafeeds. 相似文献
7.
Ichiro Nagaoka Hideki Sasahara Kei Matsushita Hideo Maeda Shuichi Fukuoka Utako Yamanouchi 《Plant Production Science》2013,16(4):546-553
ABSTRACTWe conducted a quantitative trait locus (QTL) analysis of grain appearance (GA) and agronomic traits of rice, using 128 recombinant inbred lines derived from ‘Emi-no-kizuna’ and ‘Tomohonami’. We detected two promising QTLs associated with GA: qGA4 on chromosome 4 and qGA8 on chromosome 8. qGA4 contributed highly to the greater percentage of perfect grains of the Emi-no-kizuna genotype. In the same region, we detected other QTLs associated with panicle number and spikelet number per panicle. In near-isogenic lines (NILs) in which Emi-no-kizuna alleles were introgressed in the genomic region of only the semi-dwarf 1 (sd1) locus (NIL_1) and both the sd1 locus and qGA8 (NIL_2), respectively, the percentage of perfect grains was significantly higher and the percentages of milky white, basal white, and white back grains were significantly lower than in Tomohonami; and the percentages of milky white and white back grains of NIL_2 were significantly lower than those of NIL_1. These results suggest that introgression in the sd1 region could improve GA, and that the addition of qGA8 could further improve GA. The culm lengths of the NILs were significantly shorter than that of Tomohonami, indicating improved lodging resistance. Grain weight of NIL_2 was significantly smaller than that of NIL_1, suggesting that the effect of qGA8 could be pleiotropic, or the gene that underlies qGA8 could be linked with genes associated with grain weight.Abbreviations: ANOVA: analysis of variance; AT20: mean air temperature in the 20 days after heading; BW: basal white grain; CL: culm length; DAH: days after heading; GA: grain appearance; GW: 1000-grain weight; LOD: logarithm of odds; MW: milky white grain; NIL: near-isogenic line; PG: perfect grain; PL: panicle length; PN: panicle number; PTSN: putative total spikelet number; PVE: percentage of phenotypic variation explained; QTL: quantitative trait locus; RIL: recombinant inbred line; SN: spikelet number per panicle; SNP: single nucleotide polymorphism; WB: white back grain 相似文献
8.
Pallab Kumer Sarker Shuichi Satoh Haruhisa Fukada & Toshiro Masumoto 《Aquaculture Research》2009,40(2):225-232
Non-faecal phosphorus (P) was determined for large yellowtail to estimate a minimum available P requirement (Experiment 1) and to justify inorganic P supplementation in a fish meal-based diet (Experiment 2). In Experiment 1, purified diets with incremental P concentrations were fed to yellowtail (mean weight 917 g) at a feeding rate of 1.5% of body weight. The peaks of non-faecal P excretion appeared 5–6 h after feeding in fish fed more than 4.5 g available P kg−1 dry diet. Broken-line analysis indicated that the minimum available P requirement was 4.4 g kg−1 dry diet. In Experiment 2, a purified diet (PR) containing 6.5 g available P kg−1 and a fish meal-based diet with (F1) and without (F0) additional phosphorus were fed to yellowtail (mean weight 1.1 kg) at 1.5% (PR) and 2% (F0 and F1) feeding rates respectively. There was no significant difference in P excretion between fish fed the F0 (5.5 g soluble P kg−1 dry diet) and the PR diet. However, significantly higher (34.5%) amounts of non-faecal P excretions (7.4 g soluble P kg−1 dry diet) were found in fish fed F1 compared with the F0 diet. This suggested that there was an excess of dietary P in the F1 diet and that supplementation is not needed in fish meal-based diets for large yellowtail. 相似文献
9.
Asep Ridwanudin Yutaka Haga Takayuki Katagiri Shuichi Satoh 《Aquaculture Research》2019,50(8):2218-2230
A feeding trial was conducted to evaluate the effect of nucleotides supplementation to low‐fish meal feed on growth and fatty acid composition of rainbow trout. Six isonitrogenous (42% crude protein) and isolipidic (18% crude lipid) diets were formulated containing fish meal and plant ingredients as main protein sources. The control diet was a basal diet without supplementation of nucleotides, and five experimental diets were prepared by supplementing one of the five different nucleotides in the form of 5′‐monophosphate (0.15%), that is inosine (IMP), adenosine (AMP), guanosine (GMP), uridine (UMP) and cytidine (CMP) onto basal diet. Two hundred forty juvenile rainbow trout with an initial average body weight 9.8 g were randomly distributed into twelve aquaria. After 15 weeks of feeding period, growth performance and feed utilization of rainbow trout were not significantly different among dietary treatments. Dietary GMP, UMP and CMP tended to accumulate crude lipid in the muscle and whole fish body. Moreover, dietary GMP, UMP and CMP significantly increased hepatic 18:3n‐3 and long‐chain homologue 18:4n‐3 and 20:4n‐3 contents. Hepatic 18:2n‐6 content showed also increase in fish fed GMP, UMP and CMP diets, but decreased in long‐chain homologue 20:3n‐6 and 20:4n‐6 contents. Decrease in 20:4n‐6, 20:5n‐3 and 22:6n‐3 contents was also found in the muscle of fish fed IMP, GMP and CMP diets. The present study clearly showed that there was no positive effect of dietary nucleotides on growth of fish, but dietary nucleotides particularly GMP, UMP and CMP altered polyunsaturated fatty acid composition of rainbow trout. 相似文献
10.
The morphological, physical, and mechanical properties of the nonwood plant fiber bundles of ramie, pineapple, sansevieria,
kenaf, abaca, sisal, and coconut fiber bundles were investigated. All fibers except those of coconut fiber had noncircular
cross-sectional shapes. The crosssectional area of the fiber bundles was evaluated by an improved method using scanning electron
microscope images. The coefficient factor defined as the ratio of the cross-sectional area determined by diameter measurement,
to the cross-sectional area determined by image analysis was between 0.92 and 0.96 for all fibers. This indicated that the
area determined by diameter measurement was available. The densities of the fiber bundles decreased with increasing diameters.
The diameters of each fiber species had small variation of around 3.4%-9.8% within a specimen. The tensile strength and Young’s
modulus of ramie, pineapple, and sansevieria fiber bundles showed excellent values in comparison with the other fibers. The
tensile strength and Young’s modulus showed a decreasing trend with increasing diameter of fiber bundles. 相似文献