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SR Cho SA Ock JG Yoo B Mohana kumar SY Choe GJ Rho 《Reproduction in domestic animals》2005,40(2):171-176
The present study was designed to examine the effects of cell-cycle synchronization protocols, such as confluent, roscovitine treatment and serum starvation, in bovine foetal fibroblasts on synchronization accuracy at G0/G1, viability, apoptosis, necrosis and ploidy for use as a nuclei donor. The cells in 5-10 passages were randomly allocated into three treated groups. Cells were cultured either in Dulbecco's modified Eagle's medium (DMEM) + 10% foetal bovine serum (FBS) until 90% confluent (group 1, confluent), in DMEM + 10% FBS + 30 microM roscovitine for 12 h (group 2, roscovitine), or in DMEM + 0.5% FBS for 5 days (group 3, serum starvation). Most of the cells (>80%) in all groups were arrested at the G0/G1 stage. Although the rates did not differ, cells in group 1 showed an increased cell population arrested at the G0/G1 phase. Significantly (p < 0.05) higher rates of apoptosis occurred in group 3 than in group 1 and 2 (10% vs 6% and 6%, respectively). No differences in chromosomal abnormality were observed among groups. However, by increasing the number of cell culture passages up to 15, significantly (p < 0.05) higher chromosomal abnormality was observed than in 5 and 10 passages (39% vs 28% and 23%, respectively) in group 1. The results clearly indicated that bovine foetal fibroblasts could be effectively synchronized at G0/G1 stages by all the three different treatments, confluent, roscovitine and serum starvation. However, cells in confluent showed reduced apoptosis and necrosis when they underwent 5-10 passages, exhibiting increased percentage of cells with stable chromosome diversity. Hence, cells in confluent merit further studies before they could be used as nuclear donors. 相似文献
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除草剂阿特拉津的气相色谱法残留分析 总被引:2,自引:0,他引:2
使用GC-9A气相色谱仪附氮磷检测器测定了阿特拉津在玉米植株及其土壤中的残留情况,检测极限0.05ng,变异系数小于1%。 相似文献
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PP333在苹果试管苗生根及移栽中的应用研究 总被引:2,自引:0,他引:2
以长富—2苹果试管苗为试材,研究了PP_(333)对试管苗的应用效果。结果表明:生根培养基中添加PP_(333)可抑制试管苗地上部生长,增大根/冠比值,提高根系活力,增强试管苗对移栽逆境条件的适应能力,提高移栽成活率。对长富—2苹果试管苗,PP_(333)的适宜浓度为0.3~1.0mg/L,离体繁殖成苗率比对照提高10%以上。 相似文献
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玉米灌浆后期百粒重变化的品种间差异分析 总被引:20,自引:1,他引:19
采用回归异质性测验法分析了黑龙江省8个玉米杂交种抽丝后30~60d期间百粒重的变化。结果表明,此期间早熟和晚熟杂交种百粒重变化的回归系数差异显著,因而平均灌浆速率也有显著差异。 相似文献
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