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PB Tuncer S Büyükleblebici A Eken U Taşdemir E Durmaz O Büyükleblebici E Çoşkun 《Reproduction in domestic animals》2014,49(5):746-752
The objectives of this study were to compare glycerol and ethylene glycol at different concentrations as cryoprotectants and lycopene or cysteamine (with/without) as antioxidants in Tris extender for bull semen. Twenty‐four ejaculates were obtained from three bulls. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and added using both of the cysteamine 5 mm or lycopene 500 μg/ml, and control (without additives). The addition of 7% glycerol with cysteamine, 5% ethylene glycol with cysteamine and 3% ethylene glycol with cysteamine groups gave the lowest CASA motility than the other groups. However, 7% glycerol and 7% glycerol with lycopene resulted in a better rate of CASA progressive motility compared with that of other groups. Generally, all the lycopene groups signed better protective effects on acrosome and total morphology than the other groups. Glycerol 7% and 3% ethylene glycol with lycopene groups yielded to slight higher percentages of membrane integrity assessed by HOST than that of the other groups, but 7% glycerol with cysteamine and 3% ethylene glycol with cysteamine showed the worst percentages of membrane integrity. Glycerol 7% and 5% glycerol with lycopene gave rise to a higher value of VAP, VSL and VCL compared with that of the other groups. On the contrary, adding to 5% glycerol with cysteamine showed negative effect for VAP, VSL, VCL and ALH values. All cryoprotectant groups with lycopene decreased chromatin damage than the other groups. Ethylene glycol 3% led to lower non‐return rates of inseminated cows. However, this result was not considered to be statistically important. 相似文献
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This paper provides baseline information on the involvement of women in small-scale coastal aquaculture such as the farming of slipper oyster, Crassostrea iredalei (Faustino 1933), and green mussel, Perna viridis (Linnaeus 1758). Data were gathered during a socio-economic survey of oyster and mussel farming in Western Visayas, Philippines conducted from July to December 1991.There were 175 respondents interviewed; 18.5% and 2.4% of oyster and mussel farmers were females. Fishing and related activities were the major source of livelihood for the majority of oyster and mussel farmers. In 1990, 79.4% and 69.8% of oyster and mussel farmers earned an annual income of less than P30 000 (1 USS P23). Based on the 1988 (latest data available) poverty threshold of P2654 (monthly) for Western Visayas, 80.4% and 71.7% of oyster and mussel farmers lived below poverty level in 1990. of 175 respondents. 79.3% and 48.2% of oyster and mussel farmers indicated that their household members participated in farming activities. More female household members were involved in oyster farming compared with mussel farming. Harvesting in mussel farming and both harvesting and marketing in oyster farming generated the widest participation among female household members. 相似文献
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H. P. Collins A. Alva R. A. Boydston R. L. Cochran P. B. Hamm A. McGuire E. Riga 《Biology and Fertility of Soils》2006,42(3):247-257
Sodium N-methyldithiocarbamate (metam sodium) and 1,3 dichloropropene are widely used in potato production for the control of soil-borne
pathogens, weeds, and plant parasitic nematodes that reduce crop yield and quality. Soil fumigation with metam sodium has
been shown in microcosm studies to significantly reduce soil microbial populations and important soil processes such as C
and N mineralization. However, few published data report the impact of metam sodium on microbial populations and activities
in potato production systems under field conditions. Fall-planted white mustard (Brassica hirta) and sudangrass (Sorghum sudanense) cover crops may serve as an alternative to soil fumigation. The effect of metam sodium and cover crops was determined on
soil microbial populations, soil-borne pathogens (Verticillium dahliae, Pythium spp., and Fusarium spp.), free-living and plant-parasitic nematodes, and C and N mineralization potentials under potato production on five soil
types in the Columbia Basin of Eastern Washington. Microbial biomass C was 8–23% greater in cover crop treatments compared
to those fumigated with metam sodium among the soil types tested. Replacing fumigation with cover crops did not significantly
affect C or N mineralization potentials. Cumulative N mineralized over a 49-day laboratory incubation averaged 18 mg NO3-N kg−1 soil across all soil types and treatments. There was a general trend for N mineralized from fumigated treatments to be lower
than cover-cropped treatments. Soil fungal populations and free-living nematode levels were significantly lowered in fumigated
field trials compared to cover-cropped treatments. Fumigation among the five soil types significantly reduced Pythium spp. by 97%, Fusarium spp. by 84%, and V. dahliae by 56% compared to the mustard cover crop treatment. The percentage of bacteria and fungi surviving fumigation was greater
for fine- than coarse-textured soils, suggesting physical protection of organisms within the soil matrix or a reduced penetration
and distribution of the fumigants. This suggests the potential need for a higher rate of fumigant to be used in fine-textured
soils to obtain comparable reductions in soil-borne pathogens. 相似文献
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Incorporation of triated actinomycin D into drug-sensitive and drug-resistant HeLa cells 总被引:10,自引:0,他引:10
HeLa cells, sensitive and resistant to actinomycin D, were treated with the tritiated antibiotic; only the nuclei of the drug-sensitive cells incorporated the tritium. 相似文献
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Blackmer T Larsen EC Takahashi M Martin TF Alford S Hamm HE 《Science (New York, N.Y.)》2001,292(5515):293-297
The nervous system can modulate neurotransmitter release by neurotransmitter activation of heterotrimeric GTP-binding protein (G protein)-coupled receptors. We found that microinjection of G protein betagamma subunits (Gbetagamma) mimics serotonin's inhibitory effect on neurotransmission. Release of free Gbetagamma was critical for this effect because a Gbetagamma scavenger blocked serotonin's effect. Gbetagamma had no effect on fast, action potential-evoked intracellular Ca2+ release that triggered neurotransmission. Inhibition of neurotransmitter release by serotonin was still seen after blockade of all classical Gbetagamma effector pathways. Thus, Gbetagamma blocked neurotransmitter release downstream of Ca2+ entry and may directly target the exocytotic fusion machinery at the presynaptic terminal. 相似文献
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