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71.
Optimal crop production depends greatly on available soil water, and it is therefore important to know when and how much to irrigate in order to attain agronomic potential. In this work, plant indicators are used to assess water stress. These are compared with available soil water to find the critical point for irrigation scheduling of sweet sorghum. The experimental trial was carried out in Bečej, in the Vojvodina region, on a sweet sorghum crop, grown in moderate climatic conditions on a well-drained, deep chernozemolic meadow soil. Plant indicators tested were predawn and midday leaf water potential and crop and air temperature difference. All the methods were sensitive to water deficit in plants, but not all can be used for irrigation scheduling. The predawn leaf water potential was the most reliable parameter among those tested due to its relative independence from weather conditions and a valid indicator of plant water status. This was not the case with canopy-air temperature difference. The predawn leaf water potential corelation with available soil water indicates that the threshold value for irrigation scheduling of sweet sorghum is when the former has decreased to –0.45 MPa. This corresponds to a soil water depletion to about 10% of available water in the active root zone. The canopy-air temperature difference was sufficiently sensitive to indicate the onset of mild plant water stress, although it showed a certain threshold value when water shortage appeared, which occurred when the air and canopy temperature were the same. Received: 9 April 1996  相似文献   
72.
Three adjacent EcoRI fragments of the rDNA unit from Lycopersicon esculentum, eleven anonymous genomic and two anonymous cDNA clones from Brassica napus and three restriction endonucleases: BamHI, EcoRI and EcoRV were used for RFLP analysis of the genome of Hypericum perforatum L. A polymorphic band identified with EcoRI and two rDNA probes in five somaclones originated from the same genotype was detected in all progenies of two somaclones indicating the inheritance of the molecular changes. rDNA unit heterogeneity represented by two types of RFLP pattern revealed among somaclones and seed-derived control plants using EcoRV and two rDNA probes may indicate an alloploid origin of Hypericum perforatum. The occurrence of the identical RFLP patterns in some R0 somaclones and seed-derived plants and their progenies may be related to the apomictic mode of reproduction which is assumed to be prevalent in Hypericum perforatum. The differences in RFLP patterns of progenies when compared with the maternal plants (1 out of 10 progenies of one control plant and 1 out of 8 progenies of one somaclone) may indicate that some progenies have originated through sexual recombination. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   
73.
Sulphate concentrations and 34S ratios were monitored in bulk precipitation, spruce throughfall, and soil water (depth of 30 and 90 cm) at ervená jáma (CER) and Naetín (NAC), two severely polluted sites in the Czech Republic, between December 1992 and September 1994. Throughfall [SO4 2–], up to 80 mg L–1 in winter and as low as 7 mg L–1 in summer, was higher than [SO4 2–] in bulk precipitation (annual average 6 mg L–1). There was a distinct seasonaity in S isotope abundances, with lower 34SBULK in summer (+4 per mil CER, + 6 per mil NAC) and lower 34STF in winter (+3 per mil CER, +4 per mil NAC). Wintertime 34SBULK was around +8 per mil at CER and +10 per mil at NAC, summertime 34STF was close to +7 per mil at both sites. For only a 1- month period in spring, bulk precipitation S became isotopically lighter than throughfall S. Bulk precipitation data from CER were in good agreement with those from the nearby monitoring station Lesná (LES), typically differing by less than 10 mg L–1 and 2 per mil in [SO4 2–] and 34S, respectively. Suction lysimeters (soil depth of 30 and 90 cm) yielded higher sulphate concentrations and lower 34S ratios compared to both bulk and throughfall precipitation. Little seasonality was observed in [SO4 2–] at 30 cm (around 40 mg L–1); at 90 cm [SO4 2–] was higher in winter (70 mg L–1) than in summer (45 mg L–1). 34S at 90 cm was <+5 per=" mil=" in=" 1993=" and=" up=" to=" +7.5=" in=" 1994,=" lower=" in=" the=" first=" year=" and=" higher=" in=" the=" second=" year=" compared=" to=" the=" depth=" of=" 30=" cm.=" sulphur=" fluxes=" at=" cer=" and=" nac=" are=" characterized=" by=" distinct=" isotope=" compositions=" and=" can=" therefore=" be=" used=" to=" trace=" s=" pathways=" and=" transformations=" in=" the=" forest=">  相似文献   
74.
A hybrid anion resin was tested for in situ phosphorus (P) availability measurement in soils of two stands recovering from acidification and having different P-sorption characteristics. The phosphate (P-PO4) sorption capacity of the resin (before saturation) was 48 µmol g?1. Sorption and elution were tested under P-PO4 concentrations common in acidic soils (0–0.42 mmol l?1) either with or without the presence of sulfate (0.2 mmol l?1). The efficiency of P-PO4 sorption was independent of the sulfate and was 100 ± 0.2% (n = 56, ± SD). The P-PO4 recovery stabilized after six elution steps (each: 50 ml of 0.5 M sodium hydroxide, resin/solution 5:1). The efficiency of P-PO4 recovery was 80 ± 7% and was used to evaluate field measurements. We determined the amount of P-PO4 in the field using resin bags in three consecutive years. The results indicate that bioavailable P is negatively related to the soil ability to retain P.  相似文献   
75.
Currently, the aim of modern forest management is not only timber production but also the protection of biodiversity. The initial effects of clear-cut logging on forest understory vegetation, soil and the diversity of litter beetles have been studied. We examined unstudied effects of clear-cutting disturbance on understory vascular plant species abundance, forest soils, and understory vegetation nutrients as well as beetle diversity one year after clear-cutting. Substantial changes in the prominence values and above-ground mass of forest-related vascular plant and moss species were detected. Clear-cuttings resulted in fast appearance of new light-loving plant species. The significant decrease in understory plant biomass influenced the reduction of nutrient pools in clear-cut areas after one year. The clear-cut logging negatively influenced the richness of species and the number of individuals of stenobiontic forest-related beetles in the forest litter. However, forest clear-cuttings were beneficial for eurytopic and open land species.  相似文献   
76.
During the period 2004–2015, the upper soil layer (0–20?cm) was sampled in eight agricultural production areas in Slovenia. Overall, 53, 249, 169, 139, and 413 samples were respectively taken from vegetable fields, arable cropland, hop fields, orchards, and vineyards. Copper was measured in the aqua-regia extracts of these samples using flame atomic absorption spectrometry. The minimum, maximum, average, and median concentrations were calculated for five land uses and for all eight production areas in Slovenia. Sample portions below and/or above the limit, warning, and critical concentrations of 60, 100, and 300?mg/kg, respectively, were calculated as well. The results were compared for different land uses and production areas and showed that the copper concentrations in the vegetable, arable crop, and hop fields and orchards did not exceed the critical concentration. In addition, the copper concentrations in the vineyards exceeded the critical concentration but the maximum copper concentration (508?mg/kg) was still lower than the concentrations reported from other vine-growing Mediterranean countries. Also, the copper concentrations in hop fields exhibited an increasing trend in the Celje (and Maribor) area(s).  相似文献   
77.
Twenty‐six commercial formulations of fungicides at six concentrations were evaluated in vitro for their efficacy on mycelial growth of Hymenoscyphus fraxineus (anamorph Chalara fraxinea). The results are presented as EC50, EC90 and minimal inhibitory concentration values; the comparisons with the recommended application concentrations showed that 10 of the 26 fungicides were highly effective in their ability to inhibit the mycelial growth of the pathogen. The eight most effective fungicides identified based on multiple comparisons analysis were azoxystrobin, bitertanol, captan (in two‐component preparation with trifloxystrobin), difenoconazole, kresoxim‐methyl, spiroxamine (in multicomponent preparation with tebuconazole and triadimenol), tebuconazole and trifloxystrobin. Azoxystrobin, difenoconazole, kresoxim‐methyl, mancozeb, myclobutanil, pyrimethanil, tebuconazole and trifloxystrobin were selected to verify their effectiveness in in planta tests. During two field tests in nurseries, it was found out that tebuconazole (triazole), trifloxystrobin and kresoxim‐methyl (strobilurins) and mancozeb (dithiocarbamate) were significantly effective against H. fraxineus. The best results were gained with tebuconazole, when mean percentage of diseased saplings was 16.3%, whereas in the control, it was 63.6%. The combination and alternation of fungicides from the triazole, strobilurine and dithiocarbamate chemical groups should be an effective tool for protecting ash saplings in forest nurseries. Moreover, exact timing of the treatment reflecting rainfall and development of first symptoms in the foliage will be also important.  相似文献   
78.
Dry matter partitioning and allocation is a major determinant of plant growth and its competitiveness. Common ragweed (Ambrosia artemisiifolia) is a troublesome agronomic weed species and it is also a major health concern in Europe and many other countries because of its rapid spreading and production of allergenic pollen. A field experiment was conducted in 2009 to determine the effect of nitrogen fertilizer levels (0, 100, and 200 kg ha?1) and plant density levels (1.3, 6.6, and 13.2 plants per m2) on the leaf, stem dry matter partitioning, and dry matter allocation of ragweed. With an increasing density, the stem partitioning coefficient increased, whereas the leaf partitioning coefficient decreased. The addition of nitrogen had a limited effect on the leaf and stem dry matter partitioning. The root dry matter production decreased with an increasing density and was not influenced by the addition of nitrogen. Under intraspecific competition, ragweed exhibited a stronger above‐ground competition intensity than below the ground, which resulted in a greater root : shoot ratio, compared to the low‐density stands. The level of nitrogen influenced the vertical leaf, stem, and total dry matter distribution, with a greater allocation to the top stratum of the plants, thus increasing their competition for light. The biomass allocation of ragweed to the roots in response to the nitrogen supply exhibited a low plasticity, compared to the shoots, which displayed a high plasticity. The results of this study suggested that, under intraspecific conditions and with an increasing nitrogen supply, ragweed would be more competitive in above‐ground resource acquisition.  相似文献   
79.
Two experiments were conducted on sheep to determine the effect of dietary supplementation with zinc and a medicinal plant mixture on haematological parameters and microbial activity in the rumen and large intestine. In Experiment 1, 24 male lambs were randomly divided into four groups: One group was fed an unsupplemented basal diet (control), and three groups were fed a diet supplemented with 70 mg Zn/kg diet in the form of Zn sulphate (ZnSO4), a Zn‐chelate of glycine hydrate (Zn‐Gly) or a Zn‐proteinate (Zn‐Pro), for five months. The ruminal content was collected separately from each lamb, and batch cultures of ruminal fluid were incubated in vitro with mixture of medicinal plants (Mix) with different roughage:concentrate ratios (800:200 and 400:600, w/w). Bioactive compounds in Mix were quantified by UPLC/MS/MS. In Experiment 2, four sheep were fed a diet consisting of meadow hay and barley grain (400:600, w/w), with Zn‐Gly (70 mg Zn/kg diet), Mix (10% replacement of meadow hay) or Zn‐Gly and Mix (Zn‐Gly‐Mix) as supplements in a Latin square design. Mix decreased total gas (p < 0.001) and methane (p < 0.01) production in vitro. In Experiment 1, caecal isobutyrate and isovalerate concentrations varied among the dietary treatments (p < 0.01). The isovalerate concentration of the zinc‐supplemented groups in the distal colon was higher (p < 0.001) compared with the control. In Experiment 2, the molar proportion of isobutyrate was the highest in the faeces of the sheep fed the diet with Zn‐Gly‐Mix (p < 0.01). The plasma zinc concentration was higher in the groups fed a diet supplemented with zinc (< 0.001). The haematological profile and antioxidant status did not differ between the dietary groups (p > 0.05). The diets containing medicinal plants and organic zinc thus helped to modulate the characteristics of fermentation in ruminants.  相似文献   
80.
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