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Latimer KS Bounous DI Colatos C Carmichael KP Howerth EW 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1996,25(1):23-26
Extreme eosinophilia with disseminated eosinophilic granulomatous disease is described in a 4-year-old Arabian mare. Clinical signs included weight loss, coughing, jugular distention, and ventral edema. Cutaneous lesions were not observed. Eosinophilic inflammation was observed in cytologic specimens from the respiratory tract, body cavities, and lymph nodes. At necropsy, a 20-cm diameter intrathoracic mass was observed. Smaller nodules were present in the lymph nodes, liver, spleen, adrenal glands, pancreas, and skeletal muscle. Histologically, these masses and nodules were characterized by infiltrates of eosinophils, macrophages, and multinucleated giant cells, reactive fibroplasia; and multifocal eosinophilic coagula. Microscopically, mild eosinophilic infiltrates were observed in sections of stomach, small intestine, colon, and pleura; however, gross lesions were not observed in these tissues at necropsy. The etiology of the extreme eosinophilia and disseminated eosinophilic granulomatous disease in this horse was not determined. 相似文献
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将猪生殖与呼吸综合征病毒(PRRSV)的M基因和N基因从重组质粒pMD18-T—M—N中亚克隆至pBV220原核表达栽体上,成功构建了重组表达质粒pBVM—N。将pBVM—N转化大肠埃希氏菌DH5α感受态细胞.重组菌经温度敏感诱导表达,其细菌裂解物经SDS—PAGE可检测到分子质量约为19ku的目的蛋白,与M基因表达产物一致;经蛋白质分析软件Bandscan分析,其表达量可达19.1%;Western-blotting分析表明,该重组蛋白可被兔抗PRRSV血清所识别,与预期的M基因表达产物相一致,而N基因未获表达。 相似文献
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Joiner KS Hoerr FJ Ewald SJ van Santen VL Wright JC van Ginkel FW Toro H 《Avian diseases》2007,51(3):758-763
Infectious bronchitis (IB) disease progression in vaccinated chickens after challenge was evaluated in a single commercial line of layer chickens presenting two different major histocompatibility complex (MHC) B complex genotypes. MHC B genotypes were determined by DNA sequence-based typing of BF2 alleles. In total, 33 B2/B15 and 47 B2/B21 chickens were vaccinated with an Ark-type IB virus (IBV) attenuated vaccine and challenged with Ark-type IBV field isolate AL/4614/98 14 days later. Additional chickens of both genotypes served as unvaccinated/challenged and unvaccinated/nonchallenged controls. Clinical signs, histopathologic analysis, detection of IBV genomes in tears, and IBV-specific immunoglobulin A (IgA) in tears were used to evaluate disease progression and immune response. The incidence of IBV respiratory signs was significantly higher in B2/21 than in B2/B15 MHC genotype birds. However, neither the severity and duration of respiratory signs nor the severity and incidence of histologic lesions differed significantly with MHC genotype. The levels of IBV-specific IgA in tears of vaccinated and challenged chickens did not differ significantly between MHC genotypes. IBV genomes were present in the tears of vaccinated and challenged birds, and the incidence of detectable IBV genomes did not vary significantly with MHC B genotype. From an applied perspective, these results indicate that vaccinated commercial outbred chickens with these MHC genotypes are equally resistant to IBV. 相似文献
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该书作者根据国家农业综合开发土地治理开发项目可行性研究工作的需要 在总结长期从事土地治理开发的研究成果和实践经验编写成的一本工具书。 全书共分三篇:第一篇为方法篇;第二篇为案例篇;第三篇为参考资料篇。内容包括国家近几年最近颁布的土地分类标准设计标准土壤改良指标投资估算指标生产成本分析经济效益分析常用定额计算参数及有关规定。 该书从实用性角度出发 力求通俗简明扼要 资料丰富 理论联系实际 是一本工具参考书。可供从事农业综合开发和土地管理部门的项目管理人员规划设计院 《农业工程学报》2000,16(3):31
该书作者根据国家农业综合开发土地治理开发项目可行性研究工作的需要 ,在总结长期从事土地治理开发的研究成果和实践经验编写成的一本工具书。全书共分三篇 :第一篇为方法篇 ;第二篇为案例篇 ;第三篇为参考资料篇。内容包括国家近几年最近颁布的土地分类标准、设计标准、土壤改良指标、投资估算指标、生产成本分析、经济效益分析、常用定额、计算参数及有关规定。该书从实用性角度出发 ,力求通俗、简明扼要 ,资料丰富 ,理论联系实际 ,是一本工具参考书。可供从事农业综合开发和土地管理部门的项目管理人员、规划设计院和工程咨询单位编制可… 相似文献
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金秋九月 正是农村忙着采摘丰收果实的黄金时节 也是报刊开始“播种”的季节.值此征订之际 本刊编辑部向读者热忱致意.一个刊物的个性特征 是区别于它刊的重要标志 也是衡量刊物质量的标准之一.几年来 我们注意培养本刊鲜明的泥土气息个性 突出《农村电工》的特征及好学易懂的实用风格 形成和扩大了自己相对稳定的读者群体 产生了较好的社会效应.…… 《农村电工》2000,(9):1
金秋九月,正是农村忙着采摘丰收果实的黄金时节,也是报刊开始“播种”的季节.值此征订之际,本刊编辑部向读者热忱致意. 相似文献
19.
Shivashankara KS Isobe S Al-Haq MI Takenaka M Shiina T 《Journal of agricultural and food chemistry》2004,52(5):1281-1286
Greenhouse-grown tree ripe (TR) and mature green (MG) mangoes (cv. Irwin) were exposed to high electric field treatment before 20 and 30 days of storage at 5 degrees C. MG fruits were allowed to ripen at room temperature after low-temperature storage. Fruit physical quality attributes, ascorbic acid, carotene, quercetin, total phenols, and antioxidant capacity were estimated before and after the storage period. Antioxidant capacity of fruit juice was estimated using the ferric reducing antioxidant power (FRAP) assay. Fruit firmness decreased significantly during storage. Titratable acidity decreased 20 days after storage. Total soluble solids did not change during storage. Antioxidant capacity of fruits remained unchanged up to 20 days of storage period and decreased thereafter. Total phenol and carotenes increased during storage. Antioxidant capacity of fruits was significantly correlated only to ascorbic acids. Peel color and carotenes were higher in TR fruits, whereas titratable acidity and firmness were higher in MG fruits. There was no significant difference in other parameters between the stages of picking. Electric field pretreatment affected the respiration and antioxidant capacity of TR fruits and did not have any significant affect on other parameters. TR mangoes of cv. Irwin are more suitable for low-temperature storage and can be successfully stored for up to 20 days at 5 degrees C without any significant losses in functional properties and quality attributes. 相似文献
20.
Ecological effects of climate fluctuations 总被引:3,自引:0,他引:3
Stenseth NC Mysterud A Ottersen G Hurrell JW Chan KS Lima M 《Science (New York, N.Y.)》2002,297(5585):1292-1296
Climate influences a variety of ecological processes. These effects operate through local weather parameters such as temperature, wind, rain, snow, and ocean currents, as well as interactions among these. In the temperate zone, local variations in weather are often coupled over large geographic areas through the transient behavior of atmospheric planetary-scale waves. These variations drive temporally and spatially averaged exchanges of heat, momentum, and water vapor that ultimately determine growth, recruitment, and migration patterns. Recently, there have been several studies of the impact of large-scale climatic forcing on ecological systems. We review how two of the best-known climate phenomena-the North Atlantic Oscillation and the El Ni?o-Southern Oscillation-affect ecological patterns and processes in both marine and terrestrial systems. 相似文献