Evaluation of pentoxifylline and Basal Medium Eagle supplemented to diluent on cryopreserved goat spermatozoa     

Parisa Nazari  Abbas Farshad  Asaad Vaziry  Jalal Rostamzadeh 《Reproduction in domestic animals》2020,55(10):1303-1313

This study investigated the effect of pentoxifylline (PTX) and Basal Medium Eagle (BME) on frozen–thawed goat spermatozoa. Immediately after initial examination of ejaculated semen, samples were pooled and reexamined for quality. Then, samples were divided into eight equal aliquots and diluted with a basic tris-extender containing PTX (3, 6, 9 mM) and BME (5 mM) to reach a final concentration of 25 × 10⁹ and frozen. After 24 hr, the samples were individually thawed at 37°C for 30 s and evaluated for different characteristics. Obtained post-thaw results from Computer-Assisted Sperm Analysis indicate using of 3 and 6 mM PTX led significantly to an improvement in total motility, progressive motility and velocity characteristics of spermatozoa, except the beat/cross frequency (BCF) which indicated statistically no differences (p > .05) among control and treatments. Diluents prepared with BME (5 mM) and PTX alone (3 and 6 mM) improved significantly the membrane integrity–functionality, acrosome integrity and also hyaluronidase activity. Regarding recovery rate, the results showed significantly (p < .05) higher values for diluents containing 3 and 6 mM PTX compared to other groups. Malondialdehyde concentration exhibited also a significant difference (p < .05) in diluents supplemented with 5 mM BME, 3, 6 and 9 mM PTX, and mixture of 3 mM PTX and 5 mM BME which illustrate a similarity for active mitochondria, apoptotic-like and dead spermatozoa. Finally, the ratio of sperm chromatin dispersion stained spermatozoa presented significant differences (p < .05) among treatments in which the diluents added PTX alone demonstrated significantly lower values than control and extenders containing the mixtures of BME and PTX. In conclusion, the observation in this study indicates using of 3 and 6 mM PTX and BME alone may improve significantly (p < .05) the quality of cryopreserved goat spermatozoa.  相似文献   

MINERAL ELEMENT DISTRIBUTION AND ACCUMULATION PATTERNS WITHIN TWO BARLEY CULTIVARS     

Melahat A. Birsin  M. Sait Adak  Ali Inal  Abbas Aksu  Aydin Gunes 《Journal of plant nutrition》2013,36(2):267-284

Growth stage effects on distribution of mineral nutrients or beneficial elements phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), chloride (Cl), iron (Fe), zinc (Zn), manganese (Mn), copper (Cu), molybdenum (Mo), sodium (Na), silicon (Si) and nickel (Ni), and the elements bromine (Br), rubidium (Rb), strontium (Sr), barium (Ba), lanthanum (La), cerium (Ce), and uranium (U) in two barley (Hordeum vulgare L.) cultivars and how the distribution of these elements changed were determined during the 2006–2007 growing season in a field experiment. Barley plants were sampled from the field at shooting, heading, soft dough, hard dough and harvest stages, and mineral nutrients and other elements concentrations of spike, flag leaf, old leaf, and stem samples were determined by polarized energy dispersive X-ray fluorescence (PEDXRF). Distribution patterns varied considerably from element to element. At the end of the season much of the Ca, Mg, S, Si, Fe, Mn, Cu, Ni, Sr, Ba, La, Ce, and U were located in the spikes. However, much of the P, K, Zn, Cl, Na, Br, and Rb remained in the old leaves or stem.  相似文献   

Evaluation of Water and Nitrogen Use Efficiency,Digestibility and Some Quantitative and Qualitative Characteristics of Forage Beet Cultivars Under Different Irrigation Methods and Nitrogen Levels     

Hosseini  Lida  Maleki  Abbas  Mozafari  Afshin  Mirzaeiheydari  Mohammad  Sadeghi-Shoae  Mehdi 《Gesunde Pflanzen》2022,74(1):177-191

The aim of this study was to evaluate the water and nitrogen use efficiency and some quantitative and qualitative characteristics of forage beet cultivars under the influence of different irrigation methods and nitrogen levels in two cropping years, 2017–18 and 2018–19, at Agricultural Research Station in Karaj, Iran. Experimental factors included the first factor with four irrigation methods (normal leakage, alternate furrow irrigation, fixed furrow irrigation, type (drip-strip)), the second factor was the amount of nitrogen fertilizer with three levels (150, 200 and 250?kg N ha^?1) and the third factor included three forage beet cultivars (Sbsi052, Jamon and Kyros). Among irrigation treatments, alternate furrow irrigation and fixed furrow irrigation had the highest sugar content with 9.28% and 9.17%, respectively. The highest yield of digestible organic matter was obtained in leakage irrigation treatment, nitrogen fertilizer of 250?kg ha^?1 and in Kyros at the rate of 19.45?t ha^?1. The highest yield of root digestible dry matter, potassium, sodium and free nitrogen was observed in leakage irrigation treatment and consumption of 200?kg ha^?1 nitrogen was observed in foreign cultivars. The highest crude protein was observed in alternate furrow irrigation conditions with a consumption of 200?kg ha^?1 nitrogen in cultivar Sbsi052 at 13.08%. Leakage irrigation and type tape had the highest consumption efficiency and efficiency of nitrogen uptake with application of 150 and 200?kg ha^?1 N, and the highest water use efficiency was also observed in leakage irrigation and type tape with application of 250?kg ha^?1 N in domestic and foreign cultivars. The type irrigation method showed better quantitative and qualitative yield than the furrow irrigation methods.

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Molecular and morphological characterization of Golestan (Iran) olive ecotypes provides evidence for the presence of promising genotypes   总被引：1，自引：1，他引：0  

Soraya Mousavi  Mehdi Hosseini Mazinani  Kazem Arzani  Abbas Ydollahi  Saverio Pandolfi  Luciana Baldoni  Roberto Mariotti 《Genetic Resources and Crop Evolution》2014,61(4):775-785

Unidentified olive plants naturally grow in the Golestan province of Iran, on different soils and under climates spanning from sub-temperate to desert conditions, represented by single trees or groups of few trees. We collected samples from representative sites and analyzed them by simple sequence repeat markers in order to determine their identity and their relationships to prominent Iranian and Mediterranean reference cultivars. Population structure analysis separated these ecotypes from Mediterranean and, surprisingly, from all Iranian cultivars, the parentage test excluded their direct contribution as candidate parents or offspring of cultivars, and they also showed a high level of admixture. Their differentiation from cultivated olives may be attributed to different factors: they could represent wild plants or could derive from natural dissemination of ancestral cultivated trees. Their survival up to now may be due to the fact that most of them are grown on sacred sites such as necropolis. Anyhow, the adaptation to strong environmental stresses, and their fruit size and oil content make the olive Golestan ecotypes a valuable source of genetic variation previously uncharacterized and currently threatened with extinction.  相似文献   

Inheritance and field performance of transgenic Korean Bt rice lines resistant to rice yellow stem borer   总被引：1，自引：0，他引：1  

Songjin Kim  Choljun Kim  Wonnam Li  Tokyong Kim  Yongsu Li  Mohsin Abbas Zaidi  Illimar Altosaar 《Euphytica》2008,164(3):829-839

Transgenic Korean rice plants containing the cry1Ab gene were developed for resistance against yellow stem borer (Scirpophaga incertulas, YSB). More than 100 independent transgenic lines from three Korean varieties (P-I, P-II and P-III) were generated. The amount of Cry1Ab in transgenic T₀ plants was as high as 2.88% of total soluble proteins. These levels were sufficient to cause 100% mortality of YSB larvae. The majority of T₁ transgenic lines originated from the varieties P-I and P-II followed a Mendelian fashion of segregation. Deviation from the expected segregation ratio was observed in a small number of the transgenic lines of P-I and P-II origins. However, this deviation was primarily observed in the P-III originated lines. Segregation analysis of the T₁ generation indicated that 1–3 copies of the cry1Ab gene were integrated into the genome of the majority of the transgenic lines originating from varieties P-I and P-II. Stunted and semi-fertile mutants were observed in some transgenic lines. These aberrations were either independent or closely linked to the introduced cry1Ab gene loci in different transgenic lines. Reduction in GUS expression levels and loss of toxicity against YSB larvae were found in some transgenic lines. The transgenic T₃ and T₄ lines causing 100% mortality of third instar YSB larvae in the lab were completely protected in the field. Analysis of important yield components on nine selected transgenic lines indicated that stem length, panicle length, grain number per panicle, and seed setting rates were reduced in transgenic plants compared to those in non-transgenic parental rice lines. Number of panicles per cluster, however, was significantly higher in transgenic plants. The numerical value of the average yield was in general greater in the controls than in all the transgenic lines, indicating some ‘yield drag’. Since some selected lines were highly resistant to the YSB with good yielding potential, they offer effective potential for use in insect resistance management programs.  相似文献   

利用东方黄梅和犬地卷微核千分率研究环境污染   总被引：1，自引：0，他引：1  

木合塔尔·阿不都克力木  古丽斯玛依·艾拜都拉  阿不都拉·阿巴斯 《干旱区研究》2000,17(2):50-52

本文通过地衣植物藻类细胞微核检测技术测定了分布于天山菊花台和后峡地区地衣藻类细胞微核千分率（ＭＣＮ‰）及环境污染指数,并进行了ｔ检查。结果表明（ＭＣＮ‰）有明显差异。从两个分布区２种地衣细胞观察分析来看,后峡地区地衣藻类细胞的污染指数都在３．５以上,通过ｔ检查也可以看到,差异极度显著所获得的数据表明,后峡区受到严重的污染。  相似文献   

IgG, IgM and IgA in the serum of cattle naturally infected with Mycobacterium paratuberculosis     

B Abbas  H P Riemann 《Comparative immunology, microbiology and infectious diseases》1988,11(3-4):171-175

Serum IgG, IgM and IgA antibody response in 20 cattle naturally infected with Mycobacterium paratuberculosis and in 15 non-infected cattle were measured by enzyme-linked immunosorbent assay. A strong IgG response was detected in 16 (80%) of the infected animals. Diagnostic levels of IgM were detectable in all of the infected animals as well as in 8 (53%) of the non-infected animals. Animals with paratuberculosis had a very weak specific serum IgA response and this appears to be of little value in detection of infection in these animals.  相似文献   

Identification of Aspergillus flavus and aflatoxin in home mix layer poultry feed in relation to seasons in Karachi,Pakistan     

Iram  Shajeela  Fareed  Syed Khurram  Chaudhary  Maimoona  Nisa Iqbal  Mehir un  Ghani  Rubina  Khan  Taseer Ahmed  Abbas  Tanveer 《Tropical animal health and production》2019,51(6):1321-1327

Fungal toxins in feed are leading issue in poultry industry causing a detrimental effect on the performance and health of poultry. The study was carried out to determine the incidence and concentration of the aflatoxins and their major producer Aspergillus flavus in home mix layer poultry feed in respect of seasonal variation throughout the year. A total of (n?= 204) home mix poultry layer feed samples were analyzed for the isolation of fungi. The isolates were initially screened through colony morphology and microscopic examination. However, aflatoxin concentration was determined by ELISA. Revealed results indicated that, the highest percentage of A. flavus was found during the months of June to August 50/54 (92.5%) followed by September to November 43/65 (66.1%), March to May 21/40 (52.5%), and December to February 18/45 (40%). As a whole, the incidence was recorded 132/204 (64.7%). Moreover, of the 132 samples, 41 (31%) were exceeded in respect of aflatoxin contamination from the legal limit (20 μg/kg) imposed by Food Drug Association (FDA). Statistically, the growth of A. flavus and aflatoxin production was found significantly different in respect of seasonal variation. As highest total viable fungal count (9.9?×?10⁴ CFU/g) and aflatoxin level (72.27 μg/kg) were recorded during the months of June to August and lowest in December to February. Consequently, instantaneous essential control measures are demanded regarding appropriate storage and adequate drying in post-harvesting season. Along with surveillance plans and austere regulations for monitoring the aflatoxin contents for the wellbeing of consumers.

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Comparison of immunity and resistance to diseases in male and female poultry breeders in Lebanon   总被引：1，自引：0，他引：1  

E. K. Barbour  S. K. Hamadeh  C. Hilan  S. S. Abbas 《Tropical animal health and production》1995,27(2):65-70

Summary The immune responses following vaccination and resistance to diseases were compared in male and female meat poultry breeders of the same flock. Female poultry breeders maintained antibody titres to Newcastle disease virus and infectious bronchitis virus up to the fifty-fifth day following vaccination, whereas those of the males declined significantly over the same period of time (P<0·05). In the same flock, outbreaks of Gumboro disease (60 to 62 days of age), coccidiosis (68 to 74 days of age) and aortic rupture (99 to 112 days of age) produced significantly higher losses in males. Following vaccination against fowl pox by the wing web method, 96·7% of females had a vaccine reaction (vaccine take) compared to none of the males. Immune injuries, following vaccination, were observed in 85% of the males compared to none of the females. The immune injuries included appearance of facial papules, vesicles, and reddish brown to black scabs.

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Comparacion De La Inmunidad Y Resistencia A Enfermedades En Machos Y Hembras Reproductores Aviares En El Libano

Resumen La respuesta immune después de la vacunación y la resistencia a infermidad des fue comparada en reproductores machos y hembras en un plantiel de pollos de carne. Las hembras reproductoras mantuvieron los títulos de anticuerpos contra Newcastle y bronquitis infecciosa hasta los cincuenta y cinco días después de la vacunación, mientras que los niveles de anticuerpos de los machos declinaron significativamente durante el mismo período de tiempo (P<0·05). En el mismo plantel, ocurrieron brotes de Gumboro (60 a 62 días de edad), coccidiosis (68 a 74 dias de edad) y ruptura aórtica (99 a 112 diás de edad) con pérdidas mayores en machos. Desués de la vacunación contra viruela utilizando el método escarificación en el ala membrana, el 96·7% de las hembras reaccionó inflamatoriamente, y ninguno de los machos. Las lesiones inmunes después de la vacunación, se observaron en el 85% de los machos y en ninguna de las hembras. Las lesiones inmunes consistieron en pápulas faciales, vesículas, y costras negras-rojisas.

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Comparison De L'immunite Et De La Resistance Aux Maladies Chez Des Reproducteurs Males Et Femelles Dans Un Elevage De Volailles De Chair Au Liban

Résumé Les auteurs ont comparé les réponses immunitaires post-vaccinales et la résistance aux maladies chez des reproducteurs mâles et femelles appartenant au même élevage de volailles de chair. Les reproducteurs femelles ont maintenu des titres d'anticorps contre le virus de la maladie de Newcastle et celui de la bronchite infectieuse jusqu'au 55e jour suivant la vaccination alors que ceux des mâles ont connu une baisse significative tout au long de la même période (P<0,05). Dans le même élevage, des foyers de maladie de Gumboro (sur des animaux de 60 à 62 jours), de coccidiose (de 68 à 64 jours), et des ruptures de l'aorte (animaux âgés de 99 à 112 jours), ont eu pour conséquence des pertes significativement plus élevés chez les mâles. A la suite de la vaccination contre la variole aviaire dans le tissu palmaire de l'aile, 96,78% des femelles on montré une réaction vaccinale (= prise du vaccin) aucune n'etant décelée chez les mâles. Quant aux lésions post-immunitaires, elles ont été observées sur 85% des mâles contre aucune chez les femelles. Parmi elles, on a observé l'apparition de papules faciales, de vésicules et de croûtes colorées allant du brun rouge au noir.

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Amelioration of salinity on photosynthesis and some characteristics of three rapeseed (Brassica napus) cultivars under increased concentrations of carbon dioxide     

Abbas Dehshiri  Batool Mahdavi 《Archives of Agronomy and Soil Science》2013,59(10):1423-1438

This study was done to evaluate the effects of increasing concentrations of CO₂ (CC) on rapeseed. Pot experiments were done with three cultivars (Okapi, Zarfam and RGS003) of rapeseed (Brassica napus) for salinity tolerance. Four levels of salinity (0, 5, 10 and 15 dS m^?1) were tested on the three cultivars at three CC (350, 700 and 1050 mmol L^?1) at the greenhouse of Tarbiat Modares University, Iran, during the crop seasons of 2010 to 2011. Three CCs were considered as three environments and the other two treatments (salinity and cultivar) were tested within these environments in a complete block design arranged as a factorial. Results indicated that photosynthetic rates declined with increasing levels of salinity. Elevated CC significantly increased rates of photosynthesis. The highest CC reduced the impact of salinity on photosynthesis. Increased CC reduced the rate of transpiration, which had the effects of increasing rates of photosynthesis and water use efficiency (WUE); these effects increased vegetative growth and reduced the adverse effects of salinity. Increased CC and salinity reduced harvest index. WUE increased with CC increment, and decreased with salinity elevation.  相似文献