Advances in the treatment of the cat with lymphoma in practice     

Couto CG 《Journal of Feline Medicine and Surgery》2000,2(2):95-100

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Clinicopathologic aspects of acute leukemias in the dog   总被引：1，自引：0，他引：1  

C G Couto 《Journal of the American Veterinary Medical Association》1985,186(7):681-685

Nineteen cases of canine acute leukemia were diagnosed during a 4-year period. Two main categories were identified on the basis of cytologic, hematologic, and clinical features: acute lymphoid leukemia and acute myelogenous leukemia. Clinical features included history of weight loss, anorexia, shifting limb lameness, and incoordination. Physical findings were characterized by hepatomegaly, splenomegaly, mild generalized lymphadenopathy, and pallor. Ocular lesions were found in 29% of dogs with acute myelogenous leukemia. Hematologic abnormalities included anemia, thrombocytopenia, pancytopenia, leukemia, and leukoerythroblastic reactions. Results of therapy were discouraging.  相似文献   

Myeloid and Megakaryocytic Hypoplasia in Related Standardbreds     

Catherine W. Kohn  Christine Swardson  Patricia Provost  Robert O. Gilbert  C. Guillermo Couto 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1995,9(5):315-323

Myeloid and megakaryocytic bone marrow hypoplasia in association with moderate to profound neutropenia was observed in 8 young Standardbred horses sired by the same stallion; 7 horses were intermittently thrombocytopenic. Evaluation of serial neutrophil counts in 2 horses suggested that a cyclic variation in neutrophil numbers was present, that lymphocyte numbers increased when neutrophil counts decreased, and that platelet counts decreased when neutrophil counts decreased. Preliminary bone marrow cultures indicated that myeloid progenitor cells were present and that these cells were able to respond to exogenous growth factors by differentiating. A bone marrow microenvironment or growth factor defect is suspected. Seven of 8 horses died or were euthanized. One horse with moderate neutropenia and a normal platelet count has been racing for 3 years. Necropsies in 4 horses did not reveal a cause for the myeloid hypoplasia. A familial basis for the disease is suspected.  相似文献   

Digestive enzyme activity at different developmental stages of blackspot seabream, Pagellus bogaraveo (Brunnich 1768)     

Laura Ribeiro  Ana Couto  Mercedes Olmedo  Blanca Álvarez-Blázquez  Fátima Linares  & Luísa M P Valente 《Aquaculture Research》2008,39(4):339-346

Blackspot seabream, Pagellus bogaraveo (Brunnich), has been identified as a potential species to diversify European aquaculture production. Although rearing aspects have been widely investigated, little information exists on the nutritional requirements for this species. The aim of this study was to build up information on the activity of digestive enzymes at certain developmental stages of blackspot seabream in order to understand the nutritional needs of larvae and post larvae. Fish larvae were reared from hatching to 55 days after hatching (dah), and the feeding plan consisted in rotifers (5–35 dah), Artemia naupli (30–35 dah) metanaupli (35–45) and Gemma microdiet (45–55 dah). At 7, 11, 21, 45 and 55 days after hatching (dah), pooled samples of fish larvae were collected for analysis of trypsin, amylase, lipase, alkaline phosphatase and leucine–alanine peptidase activity. Up to 21 dah, the whole larvae body was used for enzymatic analysis, whereas in older larvae only the dissected abdominal cavity was used. Blackspot seabream body dry weight growth was exponential, increasing from 60 μg at 5 dah to 30±9.7 mg at 55 dah. Amylase specific activity decreased significantly during development, exhibiting at 11 dah (0.6 U mg^?1 protein) an average value 2.7 times lower than at 7 dah, and remaining stable between 45 and 55 dah (0.7 U mg protein^?1). Trypsin specific activity remained constant until 21 dah (between 38 and 44 mU mg protein^?1), which could be related to the larvae feeding regime. At later stages of development, lipase‐specific activity exhibited a significant increase (P<0.05), being three times higher at 55 dah (8 U mg protein^?1) than at 45 dah. The total activity of the studied digestive enzymes increased significantly during larval development (until 21 dah), whereas afterwards only lipase and leucine–alanine peptidase increased significantly between 45 and 55 dah. The pattern of digestive enzymes activity was related to organogenesis and the type of food used at different developmental stages.  相似文献   

Temperature and dietary carbohydrate level effects on performance and metabolic utilisation of diets in European sea bass (Dicentrarchus labrax) juveniles     

I.S. Moreira  H. Peres  A. Couto  P. Enes  A. Oliva-Teles 《Aquaculture (Amsterdam, Netherlands)》2008,274(1):153-160

This study aimed to evaluate the effect of temperature and dietary starch level on growth performance, nutrient utilisation, whole-body composition and activity of selected hepatic intermediary metabolism enzymes in European sea bass juveniles. An experimental diet was formulated to contain 46% crude protein, 12% crude lipids and 30% pregelatinized starch (diet 30GS); two other diets were formulated to include the same levels of all ingredients as diet 30GS except for the pregelatinized starch, which was included at 20% (diet 20GS) or 10% (diet 10GS). Diets were assigned to triplicate groups of 25 fish (initial weight: 15 g) at two water temperatures (18 and 25 °C) for 10 weeks. Group receiving diet 30GS was fed to apparent satiation and groups fed diets 20GS and 10GS were pair-fed according to group fed diet 30GS, so that each group ate the same amount of all nutrients except for carbohydrates. At the end of the trial, growth rate, feed efficiency and protein efficiency ratio were higher at 25 than at 18 °C. Dietary starch level did not affect growth or N utilisation. Feed efficiency was inversely related to dietary starch level. Whole-body lipids and energy content were higher at 25 °C and increased with dietary starch levels. Whole-body protein and ash content were also higher at 25 °C but were not affected by dietary starch level. Liver glycogen, HSI and VI increased with dietary starch level. ADC of starch was higher at 25 °C and within each temperature decreased with increasing dietary starch levels. ADC of protein was neither affected by temperature nor dietary starch levels. Data on enzyme activities showed that hexokinase (HK) but not glucokinase (GK) was affected by temperature. Dietary starch level affected the activities of glucokinase and glucose-6-phosphate dehydrogenase (G6PD) but not of the others enzymes tested (HK, PK, FBPase and GDH).  相似文献   

Saccharomyces cerevisiae oxidative response evaluation by cyclic voltammetry and gas chromatography-mass spectrometry     

Castro CC  Gunning C  Oliveira CM  Couto JA  Teixeira JA  Martins RC  Ferreira AC 《Journal of agricultural and food chemistry》2012,60(29):7252-7261

This study is focused on the evaluation of the impact of Saccharomyces cerevisiae metabolism in the profile of compounds with antioxidant capacity in a synthetic wine during fermentation. A bioanalytical pipeline, which allows for biological systems fingerprinting and sample classification by combining electrochemical features with biochemical background, is proposed. To achieve this objective, alcoholic fermentations of a minimal medium supplemented with phenolic acids were evaluated daily during 11 days, for electrochemical profile, phenolic acids, and the volatile fermentation fraction, using cyclic voltametry, high-performance liquid chromatography-diode array detection, and headspace/solid-phase microextraction/gas chromatography-mass spectrometry (target and nontarget approaches), respectively. It was found that acetic acid, 2-phenylethanol, and isoamyl acetate are compounds with a significative contribution for samples metabolic variability, and the electrochemical features demonstrated redox-potential changes throughout the alcoholic fermentations, showing at the end a similar pattern to normal wines. Moreover, S. cerevisiae had the capacity of producing chlorogenic acid in the supplemented medium fermentation from simple precursors present in the minimal medium.  相似文献   

Improved evaluation of the genetic variability of Brazilian strains of Erwinia psidii with newly developed microsatellite markers     

Pollyane da Silva Hermenegildo  Lúcio Mauro Silva Guimarães  Jorge Luis Badel  Abi S. A. Marques  Acelino Couto Alfenas  Marisa A. S. Velloso Ferreira 《Plant pathology》2021,70(4):922-931

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Modelling energy utilisation in broiler breeder hens     

Rabello CB  Sakomura NK  Longo FA  Couto HP  Pacheco CR  Fernandes JB 《British poultry science》2006,47(5):622-631

1. The objective of this study was to determine a metabolisable energy (ME) requirement model for broiler breeder hens. The influence of temperature on ME requirements for maintenance was determined in experiments conducted in three environmental rooms with temperatures kept constant at 13, 21 and 30 degrees C using a comparative slaughter technique. The energy requirements for weight gain were determined based upon body energy content and efficiency of energy utilisation for weight gain. The energy requirements for egg production were determined on the basis of egg energy content and efficiency of energy deposition in the eggs. 2. The following model was developed using these results: ME = kgW0.75(806.53-26.45T + 0.50T2) + 31.90G + 10.04EM, where kgW0.75 is body weight (kg) raised to the power 0.75, T is temperature ( degrees C), G is weight gain (g) and EM is egg mass (g). 3. A feeding trial was conducted using 400 Hubbard Hi-Yield broiler breeder hens and 40 Peterson males from 31 to 46 weeks of age in order to compare use of the model with a recommended feeding programme for this strain of bird. The application of the model in breeder hens provided good productive and reproductive performance and better results in feed and energy conversion than in hens fed according to strain recommendation. In conclusion, the model evaluated predicted an ME intake which matched breeder hens' requirements.  相似文献   

Wind dispersal of Puccinia psidii urediniospores and progress of eucalypt rust          下载免费PDF全文

E. A. V. Zauza  V. M. Lana  L. A. Maffia  M. M. F. C. Araujo  R. F. Alfenas  F. F. Silva  A. C. Alfenas 《Forest Pathology》2015,45(2):102-110

To identify and implement Puccinia psidii control strategies, it is essential to understand the role of environmental factors on rust‐disease development and spread among eucalypt (Eucalyptus spp.) plantations. In this study, we evaluated the wind dispersal of P. psidii urediniospores and the progress of eucalypt rust in a field trial in Brazil. Urediniospores of P. psidii were trapped in a Burkard^® spore trap from July 2004 to June 2005. To evaluate the progress of eucalypt rust, plots were established in March 2002 using a clonal hedge scheme. The incidence of both branches and leaves with rust was assessed weekly from February 2003 to October 2005. Disease progress was studied using time‐series analysis. Urediniospores were trapped on 77% of the days sampled. The highest average urediniospore concentration was detected from July to November, and most of the urediniospores (58%) were trapped at night. Urediniospore concentration was negatively correlated with rainfall, light intensity, minimum, average and maximum temperatures and wind speed, whereas urediniospore concentration was positively correlated with leaf‐wetness duration and relative humidity. From December 2004 to June 2005, average urediniospore concentration was 0.22 spores m^?3 air h^?1 and had no correlation with meteorological data. The highest average urediniospore concentration was associated with a combination of low average temperature, low light intensity, low wind speed, high relative humidity and high leaf wetness, which reflect conditions observed at night. The disease incidence was positively correlated with the urediniospores trapped at 12 days prior to disease assessment. According to the models obtained by the time series, a seasonal yearly effect was found on rust progress. Using our models, we were also able to forecast disease incidence up to 3 months after the last field assessment.  相似文献   

The fertility capability soil classification system: Interpretation,applicability and modification     

Pedro A. Sanchez  Walter Couto  Stanley W. Buol 《Geoderma》1982,27(4):283-309

This paper summarizes the first five years of testing and evaluation of the Fertility Capability Soil Classification System (FCC) and presents an improved version, examples of interpretation and its applicability. FCC is a technical system for grouping soils according to the kinds of problems they present for agronomic management of their chemical and physical properties. It emphasizes quantifiable topsoil parameters as well as subsoil properties directly relevant to plant growth. FCC-classes indicate the main fertility-related soil constraints, which can be interpreted in relation to specific farming systems or land utilization types. Since its publication in 1975, the FCC has been tested, evaluated and put to use in various countries. As a result, the definitions of several modifiers have been changed and additional modifiers have been introduced. Soil maps can be interpreted and redrawn as FCC-units when the necessary data are available. Portions of the FAO/Unesco (1971, 1974, 1977) Soil Maps of the World have been converted to FCC-units with minor difficulties. The system is directly applicable to FAO's (1976) Land Evaluation Guidelines.  相似文献