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61.
62.
Ten wild masked palm civets infected with canine distemper virus (CDV), captured in Japan from 2005 to 2007, were histopathologically and phylogenetically analyzed. Phylogenetic analysis based on the amino acid sequences of the H protein of two CDV isolates from masked palm civets revealed that the two isolates were classified into the clade of recent isolates in Japan. Histopathologically marked lesions of virus encephalitis were present in the brain, whereas gastrointestinal lesions were absent or at a mild degree. The distribution of the lesions resembles that of recent CDV cases in dogs. Therefore, recent CDV infections in masked palm civets could be caused by recently prevalent CDV in dogs. The possibility of the masked palm civet as a spreader of CDV among wildlife is also discussed.  相似文献   
63.
We determined patterns of microsite suppression in dwarf bamboo Sasa nipponica when grazing deer were absent. This bamboo species is able to outcompete Hondo spruce (Picea jezoensis var. hondoensis) saplings under many environmental circumstances. We set up two 10 × 100 m plots inside a deer-proof fence within a subalpine forest on Mt. Ohdaigahara, central Japan, and two similarly sized plots outside the fence. Within the plots, we surveyed microsites where spruce saplings grew. We measured height and shoot elongation of all spruce saplings, and culm height and cover ratios of dwarf bamboo growing around each spruce sapling. Spruce sapling density and average height were higher inside the deer-proof fence than outside, as were bamboo height and cover. Thus, there was a negative effect of deer browsing on vegetation parameters outside the fence and a suppression of the negative effect of bamboo on spruce sapling growth inside the fence. Spruce sapling height was higher in tree-fall pits than in other microsites inside the fence, whereas both dwarf bamboo height and cover were lower in pits and rocky sites than elsewhere. In soil and collar microsites, spruce sapling shoot growth was lower and bamboo height and cover were higher than in pits and rocky sites. Inside the fence, dwarf bamboo cover was high, but pits and rocks suppressed its growth, allowing spruce saplings to flourish. To restore heavily damaged spruce forests with advanced saplings, it will be necessary to construct deer-proof fences and create and maintain microsites with pits and rocks.  相似文献   
64.
The relation between diversity of pathogenicity on clubroot-resistant (CR) cultivars of Chinese cabbage (Brassica rapa subsp. pekinensis) bred in Japan and DNA polymorphisms in 17 populations of Plasmodiophora brassicae from cruciferous plants was examined by inoculation tests and random amplified polymorphic DNA (RAPD) analysis using 18 arbitrary primers. Four pathotypes (A–D) were identified after inoculation of six CR cultivars of Chinese cabbage in the 17 populations from cruciferous crops. A relatively high level of genetic diversity was also detected among these populations in the RAPD analysis. Although the four pathotypes could not be clearly differentiated using the RAPD data, most populations of three pathotypes had a consistent location on the dendrogram. All pathotype B (virulent on five cultivars except Utage 70) and D (avirulent on all cultivars) populations, which were common in incompatible interactions with cv. Utage 70, were located in a single subcluster. All five pathotype C populations (virulent only on cv. Utage 70) except for one population grouped in another single subcluster. Because four pathotype A populations (virulent on all six cultivars, races 4 and 9) fell in different subclusters, the populations may be genetically polyphyletic. Populations from cruciferous weed Cardamine flexuosa differed remarkably from those from cruciferous crops in pathogenicity on common cultivars of Chinese cabbage and turnip and C. flexuosa, but they grouped in a single cluster with all race 9 populations from crops. Race 9 populations from crops may thus be closely related to populations from the weed rather than to races 1 and 4 from crops.  相似文献   
65.
As single superphosphate availability decreases due to closure of aging manufacturing plants increasing attention is being paid to alternative phosphorus (P) and sulfur (S) sources. This glasshouse study, conducted under non-leaching conditions was undertaken to evaluate the S supplying capacity of gypsum, S bentonite, elemental S (ES) and a range of P containing fertilizers and those where ES was added as a coating or incorporated. The magnitude of crop response in terms of shoot biomass to applied S fertilizer sources followed the order of sulfates > powdered ES > coated/incorporated ES > S/bentonite. These results indicate that fertilizers containing a mixture of sulfate and micronized ES (<75 µm), either incorporated within the granule or coated onto the fertilizer are agronomically effective. The results also suggest that local S coating of finished fertilizers is a feasible alternative to S incorporation undertaken at a central fertilizer plant.  相似文献   
66.
The main objective of this study was to evaluate the potential use of a hybrid Genetic Algorithm-Artificial Neural Network (GA–ANN) method for predicting pistachio yield and for identifying the determinant factors affecting pistachio yield in Rafsanjan region, Iran. A total of 142 pistachio orchards were selected randomly and soil samples were taken at three depths. Besides, water samples and leaves from branches without fruit were taken in each sampling point. Management information and pistachio yields were achieved by completing a questionnaire. Primarily, 58 variables affecting pistachio yield were measured, and then 26 out of them were selected by minimizing mean square error (MSE) using a feature selection (FS) method. The results showed that the accuracy of the method was acceptable. Furthermore, the sensitivity analysis showed that the main determinant features affecting the pistachio yield were the irrigation water amount, leaf phosphorus, soil soluble magnesium, electrical conductivity (EC), and leaf nitrogen.  相似文献   
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68.
Cutaneous clear cell adnexal carcinoma was found in the right lip of a 14-year-old male castrated Shih Tzu. Histologically, the tumor mostly consisted of neoplastic cells with clear or vacuolated cytoplasms and contained frequent tubular structures. Neoplastic cells showed coexpression of pan-cytokeratin (CK) and vimentin by double-labeled immunofluorescence staining. In addition, immunohistochemistry revealed that the tumor cells were positive for pan-CK (AE1/AE3, KL1, CAM 5.2), CK-7, CK-8, CK-14, CK-15, CK-18, vimentin and alpha-smooth muscle actin (SMA) with varied intensity and positivity. Among these marker proteins, SMA was positive in 75% of the tumor cells. On the other hand, CK-15, which is a specific marker of follicular stem cells, was expressed in less than 1% of the tumor cells. Based on these findings, the tumor showed diverse differentiation in apocrine sweat glands and the inner and outer root sheaths of hair follicles, indicating the follicular stem cell to be the origin of this tumor.  相似文献   
69.
70.
Abstract

In the Urubamba Valley, Peruvian Andes, we evaluated the abundance of insect pests and natural enemies in experimental plots where maize was grown either as monoculture, intercropped with beans, or intercropped with beans plus associated weeds. We also assessed the consequences of the cropping system for maize yield. The main insect pests recorded were Diabrotica spp. (Coleoptera: Chrysomelidae), Carpophilus sp. (Coleoptera: Nitidulidae) and Pagiocerus frontalis (Coleoptera: Scolytidae), and their overall abundance did not differ among crop diversity treatments. However, there was a significant adverse effect of crop diversity on the maximum abundance of both Carpophilus and Pagiocerus. The main beneficial arthropods were Paratriphleps sp. (Hemiptera: Anthocoridae), ladybirds and spiders, and their density did not differ among treatments. Maize yield did not decrease with increasing plant diversity. The reported benefits of intercropping, together with the associated efficiency in land use, make this traditional agricultural practice a valuable alternative to the use of pesticides, particularly for resource-poor Andean farmers.  相似文献   
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