首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   517篇
  免费   40篇
  国内免费   1篇
林业   46篇
农学   15篇
基础科学   1篇
  67篇
综合类   98篇
农作物   13篇
水产渔业   42篇
畜牧兽医   235篇
园艺   11篇
植物保护   30篇
  2024年   2篇
  2023年   7篇
  2022年   5篇
  2021年   22篇
  2020年   27篇
  2019年   27篇
  2018年   10篇
  2017年   24篇
  2016年   12篇
  2015年   14篇
  2014年   25篇
  2013年   28篇
  2012年   34篇
  2011年   44篇
  2010年   18篇
  2009年   15篇
  2008年   35篇
  2007年   34篇
  2006年   28篇
  2005年   32篇
  2004年   22篇
  2003年   20篇
  2002年   17篇
  2001年   5篇
  2000年   6篇
  1999年   2篇
  1998年   1篇
  1997年   5篇
  1996年   2篇
  1995年   3篇
  1994年   2篇
  1993年   2篇
  1992年   4篇
  1991年   2篇
  1990年   3篇
  1986年   1篇
  1985年   2篇
  1984年   2篇
  1983年   2篇
  1978年   1篇
  1977年   1篇
  1975年   1篇
  1971年   2篇
  1969年   1篇
  1948年   1篇
  1911年   1篇
  1906年   1篇
  1905年   1篇
  1898年   2篇
排序方式: 共有558条查询结果,搜索用时 62 毫秒
481.
Journal of Soils and Sediments - Wildfires can have major impacts on water scarcity and water quality linked to off-site transfer of polluting ash and nutrients. Understanding sediment sources in...  相似文献   
482.
The correct identification of potato varieties is crucial to maintaining the quality level of seeds produced under the Canadian Seed Potato Certification Program. During inspection of in vitro potato plant propagation centres or seed potato production field lots, morphological characteristics may not be sufficient for the identification of plantlets or tubers and therefore molecular identification is sought by inspectors for variety confirmation. With international harmonization of testing methods in mind, we proposed to evaluate further the microsatellite (SSR) markers established successfully by Reid et al. (2009, Euphytica 182: 239–249, 2011), using the reference potato variety DNA collection at the Canadian Food Inspection Agency (CFIA) established and currently used for Amplified Fragment Length Polymorphism (AFLP) genotyping. The SSR markers developed by the European community laboratories were successfully validated by and established within the CFIA laboratory. Most genotypes generated for a set of 34 varieties were identical between the 2 laboratories with only 3 discrepancies due to the different interpretation of the presence/absence of the alleles. When used with potato reference DNAs of the CFIA collection, the method successfully differentiated 217 varieties apart but 10 groups, which are most likely clonal variants, were not discriminated. The SSR markers were successfully used to address 5 potato variety verification requests from CFIA inspectors during field inspections for seed potato certification. The markers successfully confirmed the presence of rogue varieties in 4 of these requests, therefore fulfilling the CFIA’s mandate towards stakeholders of the Canadian potato industry in preserving the quality of certified seeds.  相似文献   
483.
484.
Wounds on 90 Acer rubrum trees were inoculated with Trichoderma viride in July and August 1973 to determine the effect of the fungus on the development of decay. The trees were 45 years old and 15 cm diam at 1.4 m aboveground. Each tree had 12 wounds, 1.4 cm in diam and 5 cm deep. After a year 28 trees were harvested and isolations were made from the discolored, and decayed wood associated with each wound. Trichoderma viride was reisolated from all wounds that were inoculated with it. Hymenomycetes were isolated frequently from decayed wood associated with wounds not inoculated with T. viride. No Hymenomycetes were isolated from wounds inoculated with T. viride.  相似文献   
485.
Zipf  Allan  Diner  Alex M.  Ward  Rufina  Bharara  Suman  Brown  George  Nagmani  R.  Pareek  L.K.  Sharma  Govind C. 《New Forests》2001,21(1):45-58
This report serves to evaluate possible promoters for use in theproduction of transgenic eastern white pine (Pinus strobus L.).Embryogenic cultures of eastern white pine were bombarded with goldparticles coated separately with a variety of gene constructs containingthe UidA -glucuronidase (GUS) or green fluorescentprotein (GFP) reporter gene. Transient expression of the UidAgene, driven by a novel algal virus adenine methyl transferase genepromoter, as well as five other promoters used in angiospermtransformation, were evaluated. The maize alcohol dehydrogenase promoterwas not effective in eastern white pine cultures. The construct with thedoubled Cauliflower Mosaic Virus 35S promoter plus Alfalfa Mosaic Virusenhancer showed the highest levels of expression. GUS expression wasdetected within 24 hours, but decreased after 5 days and was notdetectable 15 days after bombardment. Expression of GUS activity wasrecorded mainly in somatic embryonal heads of various stages ofdevelopment and occasionally in suspensor cells. Similar to GUSexpression, modified green fluorescent protein (GFP) was detected in theembryonal head cells 24 hours after bombardment. GFP-expressingsuspensor cells were both more infrequent and difficult to detect, astheir highly vacuolate nature rendered the GFP presence less visibleagainst the yellow background autofluorescence.  相似文献   
486.
The present work was carried out to examine the hypotensive effects of ethanolic extract (EE) from Jatropha gossypiifolia L. The oral administration of EE (125 or 250 mg kg(-1) day(-1)) caused a significant and dose-dependent reduction of the systolic blood pressure. The concentration-response curves to norepinephrine (NE) or Ca(2+) were non-parallelly shifted to the right and the maximum contractile responses were concentration dependently depressed by EE (0.1 or 0.5 mg/ml) in endothelium-deprived mesenteric artery. The cumulative additions of EE (0.1-30 mg/ml) caused a concentration-dependent relaxant response in endothelium-deprived mesenteric artery precontracted with NE or Ca(2+). In conclusion, our results have shown that the EE from J. gossypiifolia L. can elicit hypotension, by oral via, in conscious normotensive rats and vasorelaxant activity on rat mesenteric rings precontracted with NE or Ca(2+).  相似文献   
487.
A relationship was detected between the presence of embedded porcupine quills and the diagnosis of rabies in raccoons in eastern Canada during 1999-2004. No relationship was found between the presence of quills in raccoons and the diagnosis of canine distemper. Raccoons with embedded quills should be submitted for rabies testing.  相似文献   
488.
489.
Background: The importance of non-glucose carbohydrates, especially mannose and inositol, for normal development is increasingly recognized. Whether pregnancies complicated by abnormal glucose transfer to the fetus also affect the regulation of non-glucose carbohydrates is unknown. In pregnant sheep, maternal insulin infusions were used to reduce glucose supply to the fetus for both short(2-wk) and long(8-wk) durations to test the hypothesis that a maternal insulin infusion would suppress fetal mannose and inositol concentrations. We also used direct fetal insulin infusions(1-wk hyperinsulinemic-isoglycemic clamp) to determine the relative importance of fetal glucose and insulin for regulating non-glucose carbohydrates.Results: A maternal insulin infusion resulted in lower maternal(50%, P < 0.01) and fetal(35-45%, P < 0.01) mannose concentrations, which were highly correlated(r2= 0.69, P < 0.01). A fetal insulin infusion resulted in a 50% reduction of fetal mannose(P < 0.05). Neither maternal nor fetal plasma inositol changed with exogenous insulin infusions.Additionally, maternal insulin infusion resulted in lower fetal sorbitol and fructose(P < 0.01).Conclusions: Chronically decreased glucose supply to the fetus as well as fetal hyperinsulinemia both reduce fetal non-glucose carbohydrates. Given the role of these carbohydrates in protein glycosylation and lipid production, more research on their metabolism in pregnancies complicated by abnormal glucose metabolism is clearly warranted.  相似文献   
490.
Malting-quality barley samples of the varieties Harrington, Manley, and TR118, each from two locations in Saskatchewan, were collected directly from the producers and sent to China for storage. At regular intervals samples were shipped back to Canada for analysis consisting of germination studies, alpha-amylase tests, magnetic resonance imaging (MRI), and magnetic relaxation (NMR) studies. Samples showing a decrease in germinative energy and elevated levels of alpha-amylase also showed a rapid uptake of water in the area between the embryo and the endosperm as observed by MRI. Using NMR relaxation experiments, viable and nonviable barley samples could be distinguished after 2 h of imbibition.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号