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The two most common haemotropic Mycoplasma of cats, Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' have been identified using molecular techniques in all continents, except Antarctica. We report the first molecular characterization in South America of a dual infection with M haemofelis and 'Candidatus Mycoplasma haemominutum' in three domestic cats. The 16S ribosomal RNA gene was amplified in three anaemic cats in which haemoplasma organisms were seen attached to the erythrocytes in the peripheral blood smear. Bands of the expected size for M haemofelis and 'Candidatus Mycoplasma haemominutum' were observed in all three cats. The 393 bp segment of one of the amplicons had a similarity value of 100% to M haemofelis, whereas the other amplicon, a 192 bp segment, was 100% similar to 'Candidatus Mycoplasma haemominutum'. After diagnosis, two cats received blood transfusion and they were all treated with doxycycline. All three cats recovered uneventfully.  相似文献   
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Fungicides are indispensable for high-quality crops, but the rapid emergence and evolution of fungicide resistance have become the most important issues in modern agriculture. Hence, the sustainability and profitability of agricultural production have been challenged due to the limited number of fungicide chemical classes. Resistance to site-specific fungicides has principally been linked to target and non-target site mechanisms. These mechanisms change the structure or expression level, affecting fungicide efficacy and resulting in different and varying resistance levels. This review provides background information about fungicide resistance mechanisms and their implications for developing anti-resistance strategies in plant pathogens. Here, our purpose was to review changes at the target and non-target sites of quinone outside inhibitor (QoI) fungicides, methyl-benzimidazole carbamate (MBC) fungicides, demethylation inhibitor (DMI) fungicides, and succinate dehydrogenase inhibitor (SDHI) fungicides and to evaluate if they may also be associated with a fitness cost on crop pathogen populations. The current knowledge suggests that understanding fungicide resistance mechanisms can facilitate resistance monitoring and assist in developing anti-resistance strategies and new fungicide molecules to help solve this issue. © 2023 Society of Chemical Industry.  相似文献   
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Irrigation techniques that reduce water applications are increasingly applied in areas with scarce water resources. In this study, the effect of two regulated deficit irrigation (RDI) strategies on peach [Prunus persica (L.) Batsch cv. “Catherine”] performance was studied over three growing seasons. The experimental site was located in Murcia (SE Spain), a Mediterranean region. Two RDI strategies (restricting water applications at stage II of fruit development and postharvest) based on stem water potential (Ψs) thresholds (?1.5 and ?1.8 MPa during fruit growth and ?1.5 and ?2.0 MPa during postharvest) were compared to a fully irrigated control. Soil water content (θv), Ψs, gas exchange parameters, vegetative growth, crop load, yield and fruit quality were determined. RDI treatments showed significantly lower values of θv and Ψs than control trees when irrigation water was restricted, causing reductions in stomatal conductance and photosynthesis rates. Vegetative growth was reduced by RDI, as lower shoot lengths and pruning weights were observed under those treatments when compared to control. However, fruit size and yield were unaffected, and fruit quality was slightly improved by RDI. Water savings from 43 to 65 % were achieved depending on the year and the RDI strategy, and no negative carryover effect was detected during the study period. In conclusion, RDI strategies using Ψs thresholds for scheduling irrigation in mid–late maturing peach trees under Mediterranean conditions are viable options to save water without compromising yield and even improving fruit quality.  相似文献   
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The use of overhead trellis systems for the production of dry-on-vine (DOV) raisins and table grapes in California is expanding. Studies were conducted from 2006 to 2009 using Thompson Seedless grapevines grown in a weighing lysimeter trained to an overhead arbor trellis and farmed as DOV raisins for the first two years and for use as table grapes thereafter. Maximum canopy coverage for the two lysimeter vines across years was in excess of 80 %. Seasonal (15 March–31 October) evapotranspiration for the lysimeter vines (ETLys) was 952 mm in 2007 (farmed as DOV raisins) and 943 and 952 mm (when farmed as table grapes). The maximum crop coefficient (K cLys) across all 4 years ranged from 1.3 to 1.4. These maximum values were similar to those estimated using the relationship where K c is a function of the amount of shaded area measured beneath the canopy at solar noon (K c = 0.017 × percent shaded area). Covering the lysimeter’s soil surface with plastic (and then removing it) numerous times during the 2009 growing season (1 June–14 September) reduced ETLys from an average of 6.4 to 5.6 mm day?1 and the K c from 1.07 to 0.93. A seasonal basal K c (K cb) was calculated for grapevines using an overhead trellis system with a 13 % reduction in the K cLys across the growing season.  相似文献   
89.
In the High Plains, corn (Zea mays L.) is an important commodity for livestock feed. However, limited water resources and drought conditions continue to hinder corn production. Drought-tolerant (DT) corn hybrids could help maintain high yields under water-limited conditions, though consistent response of such hybrids is unverified. In this two-year study, the effects of three irrigation treatments were investigated for a DT and conventional maize hybrid, Pioneer AQUAMax P0876HR and Pioneer 33Y75, respectively. In 2013, the drier of the 2 years, irrigation amounts and crop water use (ETc) were greater for the conventional hybrid, but grain water use efficiency (WUE) and harvest index were significantly greater for the DT hybrid. In 2014, grain yields and WUE were not significantly different between hybrids. However, irrigation amounts, ETc and biomass yields were greater for the conventional hybrid. Results from both years indicate that the DT hybrid required less water to maximize grain yield as compared to the conventional hybrid. Producing relatively high yields with reduced amounts of water may provide a means for producers to continue corn production in a semiarid environment with declining water supplies.  相似文献   
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Intercropping, drip irrigation, and the use of plastic mulch are important management practices, which can, when utilized simultaneously, increase crop production and save irrigation water. Investigating soil water dynamics in the root zone of the intercropping field under such conditions is essential in order to understand the combined effects of these practices and to promote their wider use. However, not much work has been done to investigate soil water dynamics in the root zone of drip-irrigated, strip intercropping fields under plastic mulch. Three field experiments with different irrigation treatments (high T1, moderate T2, and low T3) were conducted to evaluate soil water contents (SWC) at different locations, for different irrigation treatments, and with respect to dripper lines and plants (corn and tomatoes). Experimental data were then used to calibrate the HYDRUS (2D/3D) model. Comparison between experimental data and model simulations showed that HYDRUS (2D/3D) described different irrigation events and SWC in the root zone well, with average relative errors of 10.8, 9.5, and 11.6 % for irrigation treatments T1, T2, and T3, respectively, and with corresponding root mean square errors of 0.043, 0.035, and 0.040 cm3 cm?3, respectively. The results showed that the SWC in the shallow root zone (0–40 cm) was lower under non-mulched locations than under mulched locations, irrespective of the irrigation treatment, while no significant differences in the SWC were observed in the deeper root zone (40–100 cm). The SWC in the shallow root zone was significantly higher for the high irrigation treatment (T1) than for the low irrigation treatment, while, again, no differences were observed in the deeper root zone. Simulations of two-dimensional SWC distributions revealed that the low irrigation treatment (T3) produced serious severe water stress (with SWCs near the wilting point) in the 30–40 cm part of the root zone, and that using separate drip emitter lines for each crop is well suited for producing the optimal soil water distribution pattern in the root zone of the intercropping field. The results of this study can be very useful in designing an optimal irrigation plan for intercropped fields.  相似文献   
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