Isoëtes sabatina (Isoëtaceae,Lycopodiopsida): Taxonomic distinctness and preliminary ecological insights     

Rossano Bolpagni  Sara Magrini  Andrea Coppi  Angelo Troìa  Janne Alahuhta  Marit Mjelde  Mattia M. Azzella 《水产资源保护:海洋与淡水生态系统》2021,31(10):2690-2696

1. Isoëtes sabatina is the rarest aquatic quillwort in Europe. Although recently found (2013) in Lake Bracciano (central Italy), the species is just one step away from extinction with an estimated population not exceeding 400 individuals and a spatial range of a few hundred square metres.
2. Lake Bracciano is a deep, oligo-mesotrophic Mediterranean volcanic lake that has been subjected to human activities. From January to October 2017, the lake experienced a dramatic water level decrease (up to −1.50 m), which significantly affected the littoral zone and the habitat of I. sabatina.
3. To improve the chances of survival of I. sabatina, the first eco-taxonomic investigation on this species was carried out to describe its genetic distinctness, physical and chemical requirements and companion species.
4. The phylogenetic position of I. sabatina was investigated by applying standard DNA barcoding methods. Simultaneously, during summer 2019, the physical and chemical features of water and sediments of the I. sabatina population and five small Alpine lakes colonized by Isoëtes echinospora – a supposed close relative – were characterized. These data were then compared with the available data on the trophic requirements of the target obligate aquatic Isoëtes, together with Isoëtes lacustris and Isoëtes malinverniana.
5. The present survey confirmed the taxonomic and ecological distinctness of I. sabatina – providing the first evidence of genetic differentiation from I. echinospora. Isoëtes sabatina grows in waters with temperature, conductivity and total alkalinity up to 30°C, 561 μS cm⁻¹ and 3.45 meq L⁻¹, respectively.
6. The edaphic requirements of I. sabatina confirm its outstanding conservation value, and this study offers a basic understanding of how to prevent its extinction. Now, all possible actions must be taken immediately to save this species.

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Effect of triploidy on quality traits of shi drum (Umbrina cirrosa L.) until the second rearing year     

Severino Segato  Luca Fasolato  Daniela Bertotto  Angelo Libertini  Stefania Balzan  Adolfo Corato  & Enrico Novelli 《Aquaculture Research》2007,38(1):59-65

Carcass and flesh morphometric, reological and chemical traits of triploid (3n) and diploid (2n) shi drum (Umbrina cirrosa L.) were evaluated through 7 months. Three age groups, 17‐, 21‐ and 24‐month‐old fish, were investigated. The effects of ploidy were statistically evaluated and the weight of fish was included in the model as a covariate because triploids grew less than diploids. As expected, fish weight was found to be significantly correlated with all the investigated morphometric traits, but showed a negative correlation with some chemical (pH) and colour traits (lightness) of raw fillet. In comparison with controls, triploid shi drums were characterized by different morphological traits that involved a slender body shape. In triploids, a reduction in condition factor, backbone weight, dressing index and an increase in the agility index were also recorded. When the commercial size (i.e. over 300 g) was achieved, triploids exhibited larger coelomatic and fillet (dorsal white muscle) fat deposition than diploids. Among reological traits, colour and texture were affected by ploidy; raw fillet lightness and cooked flesh tenderness were higher among triploids in all the investigated age groups. As fish were fed with a restricted feeding regimen, fillet fat deposition was supposed to be limited. Thus, the effects of ploidy on reological traits may be only partially explained by lipid fillet amount and are supposed to be more related to different fibre muscle architecture.  相似文献   

Occurrence and Concentration Levels of Nitro-PAH in the Air of Three Brazilian Cities Experiencing Different Emission Impacts     

Pérola de Castro Vasconcellos  Odon Sanchez-Ccoyllo  Catia Balducci  Rosanna Mabilia  Angelo Cecinato 《Water, air, and soil pollution》2008,190(1-4):87-94

Nitrated Polycyclic Aromatic Hydrocarbons (NPAH) were determined in the suspended particulates collected in São Paulo (Brazil) and three other cities lying in the São Paulo State, (namely, Araraquara, Piracicaba and Paulínia), during August 2002 and July 2003. São Paulo samples represented a big difference between two groups of days, one of which characterized by heavy injection of pollutants released by industrial and agricultural activities. Most NPAH congeners identified in the atmosphere were among those usually associated to diesel exhaust, with the three important exceptions of 2-nitrofluoranthene, 2- and 4-nitropyrene. Daytime reactions seemed to promote decomposition of primary fluoranthene and pyrene. Trends of NPAH at all sites seemed to be associated to regional transport of polluted air through air masses passing over sugar cane plantations of São Paulo State.  相似文献   

Plant secondary metabolites, biphenyl, and hydroxypropyl-β-cyclodextrin effects on aerobic polychlorinated biphenyl removal and microbial community structure in soils     

Wensui Luo  Elisa M. D’Angelo  Mark S. Coyne 《Soil biology & biochemistry》2007,39(3):735-743

Biphenyl and several plant secondary metabolites (PSM) (carvone, isoprene, limonene, naringin, and coumarin) and surfactant (hydroxypropyl-β-cyclodextrin, (HP-β-CD)) have been shown to improve aerobic polychlorinated biphenyl (PCB) removal by several bacterial species. The objective of this study was to determine whether these treatments also affect PCB removal and microbial community structure in a high organic matter soil (Pahokee soil series with 67% organic matter) and low organic matter soil (Woolper soil series with 6% organic matter), as determined by monitoring changes in PCB levels and phospholipid fatty acids (PLFA) profiles in laboratory microcosms amended with these compounds. Biphenyl enhanced di-chlorinated and tri-chlorinated biphenyl removal in both soils, but PSM did not improve removal of these congeners. On the contrary, HP-β-CD decreased PCB removal when used in combination with biphenyl. Two-way analysis of variance indicated that HP-β-CD significantly increased tetra- and penta-chlorinated biphenyl removal from the high organic matter soil, but not from the low organic matter soil. Principal components analysis of PLFA data indicated that HP-β-CD increased proportions of 18:1ω7c associated with Gram-negative bacteria, but decreased 10me16 and 10me17 lipid associated with Gram-positive bacteria, while biphenyl and PSMs had no detectable effects on soil microbial communities. PCB removal was not correlated to any PLFA. In conclusion, PSM previously shown to enhance PCB removal in soil-free systems were not effective in two divergent soils evaluated in this study, and HP-β-CB had increase, decrease, or no effect on PCB removal depending on types of PCB congeners, soils, and co-amendments.  相似文献   

Effect of increasing climatic water deficit on some leaf and stomatal parameters of wild and cultivated almonds under Mediterranean conditions     

Salvatore Camposeo  Marino PalascianoGaetano A. Vivaldi  Angelo Godini 《Scientia Horticulturae》2011

The prevailing environmental conditions, temperature in particular, drive seasonal changes both in leaf development and stomatal characteristics. In order to ascertain the effect of increases in climatic water deficit on some leaf and stomatal parameters under field conditions, a study was carried out on two sets of leaves (spring and summer) on a large sample of Amygdalus communis L. cultivars in comparison with several Amygdalus webbii Spach seedlings, a species more adapted to arid environments and probable ancestor of cultivated almonds. Observations were performed between spring and summer of a particularly hot season. The results showed a significant and general reduction of both leaf area and stomatal frequency and an increase in stomatal size. Nevertheless, there were evident differences between cultivated and wild almonds. A stronger reduction of leaf area was observed in A. webbii (−31%) with respect to A. communis (−14%); on the contrary, the latter reduced stomatal frequency more than the former (−25% and −19%, respectively). The examined cultivated almonds, in response to the increase in climatic water deficit, tended to arrange their stomatal structures like those of wild almonds. Finally, increasing the climatic water deficit, the slope of the linear regressions between stomatal frequency and size did not change in either species, leading to a better understanding of the mechanisms of almond acclimation to environmental stresses.  相似文献   

Use of electrochemical biosensor and gas chromatography for determination of dichlorvos in wheat     

Longobardi F  Solfrizzo M  Compagnone D  Del Carlo M  Visconti A 《Journal of agricultural and food chemistry》2005,53(24):9389-9394

Two methods for the determination of dichlorvos in durum wheat by electrochemical assay and gas chromatography, respectively, have been developed. Dichlorvos, an organophosphorus anticholinesterase pesticide, was extracted from wheat with hexane, and the filtered extract was directly analyzed by gas chromatography with nitrogen-phosphorus flame detection (NPD). Recoveries of dichlorvos from milled wheat spiked at 0.25-1.5 microg/g ranged from 96.5 to 100.9%, and the limit of detection was 0.02 microg/g. The electrochemical assay was based on the detection of choline, the acetylcholinesterase product, via a choline oxidase biosensor. An aliquot of the filtered hexane extract was partitioned with phosphate buffer solution, and the organic layer was evaporated prior to electrochemical analysis. A limit of detection of 0.05 microg/g of dichlorvos was obtained with mean recoveries of 97-103% at spiking levels of 0.25-1.5 microg/g. A good correlation (r = 0.9919) was found between the results obtained with the electrochemical and those obtained with the gas chromatographic methods. The electrochemical method was peer-validated in two laboratories that analyzed 10 blind samples (5 duplicates), including a blank and 4 spiked samples with dichlorvos at levels of 0.25, 0.60, 1.00, and 1.50 microg/g. Within-laboratory repeatability (RSDr) and between-laboratory reproducibility (RSDR) ranged from 5.5 to 7.8% and from 9.9 to 17.6%, respectively.  相似文献   

Genotoxic activity of dichlorvos,trichlorfon and dichloroacetaldehyde     

Gabriele Aquilina  Romualdo Benigni  Margherita Bignami  Angelo Calcagnile  Eugenia Dogliotti  Emiliana Falcone  Angelo Carere 《Pest management science》1984,15(5):439-442

Two structurally related organophosphorous insecticides, dichlorvos and trichlorfon, and their main metabolite dichloroacetaldehyde, were assayed for their ability to induce DNA repair, detected as unscheduled DNA synthesis in human epithelial-like cell (EUE) cultures in vitro. A dose-response relationship was found for treatment with the two pesticides, but negative results were obtained with dichloroacetaldehyde. Tests for induction of gene mutation, as ouabain resistance in Chinese hamster cells (V79), failed to show any mutagenic activity by these compounds.  相似文献   

Synthesis and juvenile hormone activities of some new ether derivatives of hydroquinone     

Pietro Massardo  Franco Bettarini  Paolo Piccardi  Angelo Longoni 《Pest management science》1983,14(5):461-469

A new class of compounds, derived from hydroquinones, has been synthesised and bioassayed for juvenile hormone activity on yellow mealworm (Tenebrio molitor), confused flour beetle (Tribolium confusum), and mediterranean flour moth (Anagasta kuehniella). Structure-biological activity relationships of the most significant products are tabulated and discussed. Two of the new compounds, 1, 4-bis(5-chloropent-4-ynyloxy) benzene (I) and 1-(5-chloropent-4-ynyloxy)-4-phenoxybenzene (II), showed outstanding activity in morphogenetic assay with T. molitor, and good field persistence. Product II is of particular interest for the control of various Homopteran species, mosquitoes, stored product pests and fire ants.  相似文献   

New chlorinated juvenile hormone analogues and their biological activity     

Paolo Piccardi  Pietro Massardo  Franco Bettarini  Angelo Longoni 《Pest management science》1980,11(4):423-431

In the search for new juvenile hormone analogues (JHAs), a general approach was chosen based on the substitution of the isoprene unit, that carries the epoxy function in the natural hormone JHIII, by 3,3,3-trichloropropyl, 2,2-dichlorocyclopropyl- methyl, 3,3-dichloroallyl, or 3-chloroprop-2-ynyl groups. Accordingly, several terpenoid aromatic JHAs were prepared with, or without, oxygen atoms in the aliphatic chain. Structure-biological activity relationships of the most active compounds toward the yellow mealworm (Tenebrio molitor) and the large cabbage white butterfly (Pieris brassicae) are given. With a view to preparing synthetically accessible analogues, some alkyl phenyl ethers were also made, which were not branched in the aliphatic chain and were related terminally to the most active compounds. They were only moderately active against insects when tested for morphogenetic activity, but exhibited a good field persistence. The occurrence of a moderate acaricidal activity among these analogues prompted the examination of several 1,4-disubstituted-phenyl derivatives. The ovicidal effectiveness of these products against a susceptible strain of the two-spotted spider mite, Tetranychus urticae, was ten or 100 times higher than that of the well known and highly potent JHAs.  相似文献   

Repair of wing fracture in a fowl     

S J Angelo  Y S Bhatia 《The Indian veterinary journal》1970,47(12):1127-1129

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