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101.
Dorgival Morais de Lima Jr. Francisco Fernando Ramos de Carvalho Maria Norma Ribeiro Ângela Maria Vieira Batista Bárbara Ferraz Ferreira Paulo de Barros Sáles Monteiro 《Tropical animal health and production》2014,46(6):995-1000
The use of native and adapted forages is an alternative for sustainable production systems in northeast Brazil. The goal of this study was to evaluate the substitution of Tifton 85 by maniçoba hay on the performance and carcass traits of Morada Nova hair sheep. The 16 lambs that were used in this study initially weighed 19.36?±?1.48 kg, were housed in individual pens, and were slaughtered at 58 days of confinement. The dry matter intake (839.84 g/day and 3.81 % WB) was higher (P?<?0.05) in sheep fed maniçoba hay. The feed conversion was 7.39 for sheep fed maniçoba hay. The empty body weight (21.61 kg) and cold carcass weight (11.40 kg) did not differ (P?>?0.05) between sheep fed different hay types. The replacement of Tifton 85 by maniçoba hay did not influence the loin eye area (cm2) or carcass compactness index (kg/cm). The replacement of Tifton 85 by maniçoba hay in Morada Nova sheep diet did not affect the weight gain or carcass characteristics. The maniçoba hay can be considered a forage resource for feeding sheep in Brazilian semiarid tropics. 相似文献
102.
Liliana R. Leite-Martins Maria I.M. Mahú Ana L. Costa Ângelo Mendes Elisabete Lopes Denisa M.V. Mendonça João J.R. Niza-Ribeiro Augusto J.F. de Matos Paulo Martins da Costa 《Preventive veterinary medicine》2014
Antimicrobial resistance (AMR) is a growing global public health problem, which is caused by the use of antimicrobials in both human and animal medical practice. The objectives of the present cross-sectional study were as follows: (1) to determine the prevalence of resistance in Escherichia coli isolated from the feces of pets from the Porto region of Portugal against 19 antimicrobial agents and (2) to assess the individual, clinical and environmental characteristics associated with each pet as risk markers for the AMR of the E. coli isolates. 相似文献
103.
Jessica Maria Leite dos Santos Jomar Patrício Monteiro Wesley Lyeverton Correia Ribeiro Iara Tersia Freitas Macedo Ana Lourdes Fernandes Camurça-Vasconcelos Luiz da Silva Vieira Claudia Maria Leal Bevilaqua 《Veterinary parasitology》2014,199(3-4):160-164
Haemonchus contortus is the most prevalent nematode in Brazil. The objective of this study was to select 6 populations of H. contortus of known or suspected benzimidazole resistance status and characterize these using quantitative real-time polymerase chain reaction (qPCR) for single nucleotide polymorphisms (SNPs) F200Y, F167Y and E198A in the β-tubulin isotype 1 gene. qPCR was performed using DNA from a pool of 10 adult male H. contortus from a single animal per farm. Faecal egg count reduction test (FECRT) and egg hatch test (EHT) were used to determine the resistance status. Samples were obtained from 6 farms located in 5 counties in the Ceará State: Tauá, Boa Viagem, Quixadá, Santa Quitéria and Solonópole. The inbred-susceptible-Edinburgh (ISE) isolate was used as reference for comparative purposes in the qPCR. Benzimidazole resistance was detected by FECRT on all farms with efficacy values ranging from 0 to 51%. EC50 values as determined by EHT were all above 1.49 μg/ml. High frequencies of the resistant SNPs F200Y and F167Y alleles were detected but no resistance was detected at SNP E198A. Our results suggest that the SNPs F167Y and F200Y are both important for benzimidazole resistance in the studied populations. 相似文献
104.
Ragnhild J Bager Egle Kudirkiene Isabelle da Piedade Torsten Seemann Tine K Nielsen Susanne E Pors Andreas H Mattsson John D Boyce Ben Adler Anders M Bojesen 《Veterinary research》2014,45(1)
The Gram-negative bacterium Gallibacterium anatis is a major cause of salpingitis and peritonitis in commercial egg-layers, leading to reduced egg production and increased mortality. Unfortunately, widespread multidrug resistance and antigenic diversity makes it difficult to control infections and novel prevention strategies are urgently needed. In this study, a pan-genomic reverse vaccinology (RV) approach was used to identify potential vaccine candidates. Firstly, the genomes of 10 selected Gallibacterium strains were analyzed and proteins selected on the following criteria; predicted surface-exposure or secretion, none or one transmembrane helix (TMH), and presence in six or more of the 10 genomes. In total, 42 proteins were selected. The genes encoding 27 of these proteins were successfully cloned in Escherichia coli and the proteins expressed and purified. To reduce the number of vaccine candidates for in vivo testing, each of the purified recombinant proteins was screened by ELISA for their ability to elicit a significant serological response with serum from chickens that had been infected with G. anatis. Additionally, an in silico prediction of the protective potential was carried out based on a protein property prediction method. Of the 27 proteins, two novel putative immunogens were identified; Gab_1309 and Gab_2312. Moreover, three previously characterized virulence factors; GtxA, FlfA and Gab_2156, were identified. Thus, by combining the pan-genomic RV approach with subsequent in vitro and in silico screening, we have narrowed down the pan-proteome of G. anatis to five vaccine candidates. Importantly, preliminary immunization trials indicated an in vivo protective potential of GtxA-N, FlfA and Gab_1309.
Electronic supplementary material
The online version of this article (doi:10.1186/s13567-014-0080-0) contains supplementary material, which is available to authorized users. 相似文献105.
A. L. Lourenço A. Dias‐da‐Silva A. S. Santos M. A. M. Rodrigues J. W. Cone L. M. M. Ferreira 《Journal of animal physiology and animal nutrition》2014,98(3):453-457
The digestive capacity of the Portuguese native breed of Barrosão cattle and the Holstein–Friesian breed was measured when fed meadow hay (72–74 g CP and 641–671 g NDF/kg dry matter), offered either alone or supplemented with soya bean meal (150 g/kg dry matter), at maintenance level. Four mature cows of each breed were used. Average initial live weight (LW) was 457 and 635 kg for the Barrosão and the Holstein–Friesian cows respectively. The organic matter digestibility (OMD) of the meadow hay was higher in Holstein–Friesian than in Barrosão cows (p < 0.08) whether supplemented or not. The neutral detergent fibre digestibility of the hay was also higher in Holstein–Frisian when the hay was fed alone (p < 0.08). The soya bean meal supplementation increased the OMD and the NDFD digestibility of the total diet (p < 0.05), but not the OMD digestibility of the hay (p > 0.05). The results obtained in this study suggest a higher ability to digest fibre in the large dairy breed than in the small native breed. This suggestion is also supported by previous findings with sheep breeds largely differing in mature live weight. 相似文献
106.
A. C. O. Dearo G. de Moraes Marcondes J. C. O. Araújo C. E. S. Grumadas P. C. Marino W. T. Teixeira G. de La Penha Chiacchio Fernandes 《Equine Veterinary Education》2014,26(12):640-644
Strangulating obstruction of the small intestine by anomalous mesenteric bands is an uncommon cause of colic in horses. The most commonly reported bands are mesodiverticular bands that are embryological remnants of the vitelline arteries. Despite the congenital nature of the anomaly, a wide age range of horses can be affected. This report describes a case of small intestinal strangulating obstruction caused by an unusual, anomalous congenital band attached to the ileocaecal fold in a yearling colt. Information including case background, history, clinical, laboratory, surgical and pathological findings is described. 相似文献
107.
Anne-Sophie Sergent Nathalie Bréda Léopoldo Sanchez Jean-Charles Bastein Philippe Rozenberg 《Annals of Forest Science》2014,71(6):709-720
Context
Since the 2003 drought and heat wave, Douglas-fir dieback has been reported in France in trees older than 30 years. Consequently, it is questioned whether selected Douglas-fir provenances are suited to the frequent and severe drought events which are forecast due to climate change.Aims
Our objective was to contribute to the screening of variability in productivity and growth response to soil-water deficit of mature trees from provenances not currently used for plantation in France.Methods
We sampled 22 provenances, including coastal and interior Douglas-fir, covering a wide part of its natural distribution, from Oregon to California for coastal provenances and from British Columbia to New Mexico for interior provenances. These provenances were planted at the mid 1970s in two provenance trials located in the south-west area of France. Variability of productivity, of wood density, and of radial growth in response to drought episodes among provenances was quantified and related to soil-water deficit computed by daily water balance calculations.Results
Whatever the provenance, annual radial growth is highly dependent on local soil-water deficit (Felines R 2 = 0.57, Le Treps R2 = 0.49). Radial growth and wood properties exhibit large differences between provenances at 30 years old. Variability between provenances for all wood characteristics studied is mainly structured geographically. Coastal provenances perform best for productivity at 30 years old (619 cm2?±?59), and exhibit a small growth reduction in 2004, the second successive year of drought (?10.7 %?±?3.8). Surprisingly, the southern interior provenances from the driest environments in the natural range show a large growth reduction in 2004 (?30.5 %?±?5.2).Conclusions
The provenances tested exhibited significant differences in growth performance and growth reduction induced by the soil-water deficit. The approach coupling retrospective analysis of radial growth on mature trees and water balance modelling is relevant for evaluating provenance adaptation to more frequent or severe drought episodes. 相似文献108.
The present study is a first attempt at evaluating the effects of repeated disturbance, such as that caused by mechanical fishing systems, on the target species, Tapes philippinarum, intensively exploited in the Lagoon of Venice (North-East Italy). In particular, in free-access fishing grounds, detrimental effects of dredging may be suffered by under-sized clams, which are disturbed by fishing tools many times throughout their lives before they are collected. Similar mechanical stress conditions are experienced by T. philippinarum in licensed areas, where they are farmed in aquaculture conditions and undergo the harmful impact of hydraulic dredging. To evaluate the effects of short-term mechanical stress, under-sized clams were subjected to experimental shaking in the laboratory, and detrimental effects on their well-being were investigated by applying the biomarker approach. Changes in physiological, biochemical and behavioural responses were evaluated by determining scope for growth, adenylate energy charge, survival in air time, reburrowing time, and shell damage level. Responses highlighted general worsening in clam condition as mechanical stress increased. Among the various measurements, survival in air and reburrowing time appeared particularly suitable as indices, their responsiveness and applicability suggesting their use in assessing mechanical stress due to dredging in field conditions. 相似文献
109.
A. Espinach Ros V.G. Amutio J.P. Mestre Arceredillo G. Orti A. Nani 《Aquaculture (Amsterdam, Netherlands)》1984,37(2):141-146
Pituitary suspensions from the characin, Prochilodus platensis, were injected intraperitoneally into male and female Rhamdia sapo at doses between 0.37 and 6 mg dry weight per kg of body weight. Doses from 0.75 to 6.0 mg/kg effectively induced ovulation. The latent period between injection and ovulation for females held at different temperatures (17 to 27°C) decreased with increasing temperature.Stripping of gametes from both sexes, and dry method fertilization were successful and produced viable eggs. The period between ovulation and stripping for the highest hatching rates decreased from about 9 h at 20°C, to 5 h at 24°C. No viable eggs remained in samples obtained after 15 h at 20°C, or 8 h at 24°C. Hatching percentage and the fraction of deformed fry were negatively correlated (r = ?0.75; P < 0.001). 相似文献
110.
Maturation and ovulation were induced in female Prochilodus platensis with multiple homologous pituitary extract injections (total dose 0.9 to 1.4 hypophysis/kg) or single injections of pituitary extract combined with human chorionic gonadotrophin ((1 hypophysis + 500 I.U.)/kg). One of the females given the latter treatment produced viable eggs. Latency time from injection to ovulation was 9 h (at 28°C). Hatching occurred 12 h after fertilization. Survival after 30 days of rearing was 96%. 相似文献