SAGE transcript profiling of the juvenile cambial region of Eucalyptus grandis     

Gallo de Carvalho MC  Caldas DG  Carneiro RT  Moon DH  Salvatierra GR  Franceschini LM  de Andrade A  Celedon PA  Oda S  Labate CA 《Tree physiology》2008,28(6):905-919

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Isolation and purification of a low molecular weight protein from bovine urine     

S Kawamura  H Inoue  T Oda  N Itoh  S Higuchi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(4):787-793

A low molecular weight protein was separated from urine samples obtained from a heifer with spontaneous renal disease and from cows with CaNa2EDTA-induced renal dysfunction. The molecular weight and electrophoretic mobility of the separated protein were examined. The low molecular weight protein collected by gel filtration chromatography was further separated into two fractions by ion exchange chromatography using DEAE-cellulose. One of the two fractions, the lowest molecular weight protein showed a single band in SDS-PAGE, and its molecular weight was approximately 12,000. An antiserum against this protein formed a single precipitin line with the urine from cows with experimentally induced renal dysfunction and a heifer with spontaneous renal disease by the double immunodiffusion technique. However, the antiserum did not form any precipitin line with the concentrated urine of healthy cow and human beta 2-microglobulin. In cellulose acetate membrane electrophoresis, this protein migrated in the same position as that of serum gamma-globulin from healthy cow.  相似文献   

Ascorbic acid promotes graft-take in sweet pepper plants (Capsicum annuum L.)     

Masahumi Johkan  Masayuki Oda  Genjiro Mori 《Scientia Horticulturae》2008,116(4):343-347

The cause of the low survival rate of sweet pepper plants after grafting was investigated and compared with findings of eggplant and tomato plants, and the promotive effect of ascorbic acid (AA) was determined. Sweet pepper plants formed less callus at the cut surfaces of the stem compared to eggplant and tomato plants. Foliar application with 100 mg L⁻¹ AA promoted callus formation at the cut surfaces of the scion stems and improved the survival rate after grafting. We suggest that the low survival rate of grafted sweet pepper plants is caused by their low rate of callus formation, and that AA can be used to promote graft-take through the acceleration of callus formation at the cut surfaces of the stems.  相似文献   

Potassium distribution in black heartwood of sugi (Cryptomeria japonica) I: Localization in axial parenchyma cells     

Hiroshi Matsunaga  Rika Shiotari  Junji Matsumura  Kazuyuki Oda  Yasuhiro Utsumi  Koichi Yamamoto 《Journal of Wood Science》2006,52(2):95-100

This is the first report to ascertain potassium location in black heartwood of sugi (Cryptomeria japonica). The objective of this study was to understand the distribution of potassium in sugi black heartwood in connection with anatomical morphology. Scanning electron microscopy with energy dispersive X-ray analysis (SEM-EDXA) was used to investigate the distribution of inorganic elements, especially potassium. In black heartwood, potassium was detected in all tissues (tracheids, ray parenchyma, and axial parenchyma), but its concentrations were different in each xylem tissue. Potassium was particularly accumulated in the droplets in axial parenchyma cells. In addition, calcium was also detected in all tissues. Phosphorus sometimes existed in the granules in ray parenchyma cells. From optical microscopy, the axial parenchyma cells were observed to be arranged in a tangential direction on transverse section and the droplets in these cells were distributed in areas of high ash and green moisture content. For red heartwood, potassium detected by SEM-EDXA was far lower in all tissues than in the black heartwood, while the trend for calcium was the converse. Potassium in axial parenchyma cells was more abundant in black heartwood than in typical pale red heartwood.  相似文献   

Effects of melophlins on colony formation of Chinese hamster V79 cells and IL-8 production in PMA-stimulated HL-60 cells          下载免费PDF全文

Oda T  Fujita A  Xu J  Mochizuki M  Ukai K  Namikoshi M 《Marine drugs》2007,5(1):1-5

We have recently isolated four new melophlins P (1), Q (2), R (3), and S (4) together with seven known melophlins A (5), D (6), E (7), G (8), H (9), I (10), and O (11) from two marine sponges of the genus Melophlus collected in Palau. In this study, the influence of these compounds on the colony formation of Chinese hamster V79 cells and the production of IL-8 in PMA-stimulated HL-60 cells were examined. These 11 compounds did not show any effect on IL-8 production. The EC₅₀ values of compounds 2, 3, 4, 5, 7, 9, 10, and 11 against V79 cells were 44.0, 13.3, 16.7, 27.2, 19.8, 8.5, 23.1, and 9.6 μM, respectively. The linear-chain-type compounds (1, 6, and 8) were not active against V79 cells at 50 μM. Although the growth inhibitory activity of these melophlins was not remarkable, some structure-activity relationships of these compounds against V79 and murine leukemia L1210 cells were observed.  相似文献   

Quantitative in vivo measurement of central benzodiazepine receptors in the brain of cats by use of positron-emission tomography and [11C]flumazenil   总被引：1，自引：0，他引：1  

Shimada Y  Kiyosawa M  Nariai T  Oda K  Toyama H  Ono K  Senda M  Ishiwata K 《American journal of veterinary research》2003,64(8):999-1002

OBJECTIVE: To map central benzodiazepine receptors (BZRs) in the brain of cats by use of positron-emission tomography (PET) and [11C]flumazenil. ANIMALS: 6 male cats that weighed between 2.0 and 3.6 kg. PROCEDURE: Brain images obtained by PET evaluation of [11C]flumazenil were superimposed on T2-weighted magnetic-resonance imaging (MRI) scans of the same cats. Detailed anatomic regions, such as the cerebral cortex, striatum, thalamus, midbrain, and cerebellum, on the PET images were evident by PET-MRI registration. Regional binding of [11C]flumazenil to BZRs was quantitatively measured by use of a model with 2 tissue compartments and 4 variables. RESULTS: The highest value for distribution volume was observed in the cerebral cortex, and the lowest value was found in the midbrain of cats. CONCLUSIONS AND CLINICAL RELEVANCE: Binding of [11C]flumazenil to BZRs in the brain of cats can be quantitatively measured by use of PET with the aid of PET-MRI registration. It is difficult to diagnose changes in these neuroreceptors within the field of current veterinary science. In the future, PET should prove useful for investigating and diagnosing brain disorders in animals in clinical settings.  相似文献   

稻田中肥料 Cd的吸收和淋溶     

王慎强  H.Oda  A Kawasaki  陈怀满  周东美  郑春荣 《农业环境科学学报》2005,24(2):412-412

20世纪 60年代发生在日本的"骨痛病"( itai itai)是由当地居民食用因矿山开采而导致 Cd污染的大米引起的,因此,稻米中 Cd的含量一直是人们关心的问题.稻米中 0.2 mg· kg-1 Cd 含量标准已被 Codex建议,这一标准在我国也已沿用许久.目前,减少 Cd在水稻中累积的技术开发方兴未艾.本文采用田间 Lysimeter研究了两种土壤中水稻对肥料 Cd的吸收和肥料 Cd的淋溶.  相似文献   

Quantitative diversity of 67 kda protective antigen among serovar 2 strains of Erysipelothrix rhusiopathiae and its implication in protective immune response     

Kitajima T  Oishi E  Amimoto K  Satoshi U  Nakamura H  Oda K  Katayama S  Izumida A  Shimizu Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(10):1073-1077

Mouse monoclonal antibodies (MAbs), raised against the NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain Kyoto (serovar 2), recognized two different epitopes on a single protein of molecular weight 67 kDa. The MAbs were classified as protective or non-protective against strain Fujisawa (serovar 1). In immunoblotting analysis using the MAbs, fifteen wild strains were shown to contain different amounts of 67 kDa protective antigen. Each formalin-killed whole cell vaccine (bacterin) prepared from the fifteen wild strains conferred different levels of protection against strain Fujisawa in mice. Bacterins prepared from wild strains with larger amounts of 67 kDa protective antigen tended to give high levels of antigen-specific antibody and better protection to mice. These results indicate that the amount of 67 kDa protective antigen which influences the induction of protective immune responses may vary substantially among the strains of E. rhusiopathiae (serovar 2).  相似文献   

Optimal conditions for visualizing water-conducting pathways in a living tree by the dye injection method     

Umebayashi T  Utsumi Y  Koga S  Inoue S  Shiiba Y  Arakawa K  Matsumura J  Oda K 《Tree physiology》2007,27(7):993-999

To elucidate the water-conducting pathways in living trees by the dye injection method, suitable sample preparation procedures are needed. We evaluated quantitatively the properties and concentrations of three dyes (acid fuchsin, basic fuchsin and safranin) widely used for this purpose, and determined the optimal conditions required to avoid artifacts after dye injection into the sap stream of Pieris japonica D. Don. Among the dyes tested, an aqueous solution of acid fuchsin at a concentration of 0.1% or more was the most useful for delineating water movement. In non-transpiring stem segments, the vertical movement of acid fuchsin by capillarity and diffusion from the dye injection site was limited. However, acid fuchsin moved rapidly in the horizontal direction by capillarity and diffusion, and most xylem cells were stained within 2 h. A delay of more than 2 h between dye injection and examination of the tissues greatly reduces the precision of the method. Use of the dye injection method without appropriate, well-defined experimental procedures may give rise to misleading information about the functional water-conducting pathway in living trees.  相似文献   

Isolation and Characterization of the Unit of Electron Transfer in Heart Mitochondria     

Green DE  Blair PV  Oda T 《Science (New York, N.Y.)》1963,140(3565):382

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