全文获取类型
收费全文 | 95篇 |
免费 | 5篇 |
专业分类
林业 | 17篇 |
农学 | 6篇 |
7篇 | |
综合类 | 6篇 |
农作物 | 14篇 |
水产渔业 | 11篇 |
畜牧兽医 | 34篇 |
园艺 | 4篇 |
植物保护 | 1篇 |
出版年
2021年 | 2篇 |
2018年 | 2篇 |
2017年 | 3篇 |
2015年 | 4篇 |
2014年 | 2篇 |
2013年 | 9篇 |
2012年 | 5篇 |
2011年 | 11篇 |
2010年 | 2篇 |
2009年 | 10篇 |
2008年 | 6篇 |
2007年 | 7篇 |
2006年 | 8篇 |
2005年 | 7篇 |
2004年 | 1篇 |
2003年 | 2篇 |
2002年 | 1篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1995年 | 1篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1984年 | 2篇 |
1973年 | 1篇 |
1965年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有100条查询结果,搜索用时 15 毫秒
51.
A sesquiterpene quinone, 5-Epi-smenospongine, promotes TNF-α production in LPS-stimulated RAW 264.7 Cells
下载免费PDF全文
![点击此处可从《Marine drugs》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Eight sesquiterpene quinones: ilimaquinone (1), smenospongidine (3), smenospongiarine (5), smenospongine (7), and their corresponding 5-epimers 2, 4, 6, and 8, isolated from the Palauan marine sponge Hippospongia sp., were examined regarding their effects on TNF-α production in LPS-stimulated RAW 264.7 cells. 5-Epi-smenospongine (8) promoted the production of TNF-α to a level three times greater than the control at 10 μM, but compounds 1–7 did not show apparent activity. The results suggest that the cis-decaline ring and a primary amine in the benzoquinone ring are necessary for activity. This is the first study to report the modulation of TNF-α production by a sesquiterpene quinone. 相似文献
52.
53.
Masatoshi Miyazaki Junko Obata Yoshiko Iwamoto Tatsuya Oda Tsuyoshi Muramatsu 《Fisheries Science》2001,67(5):956-964
ABSTRACT: Poly(α- L -guluronate)lyase, as one of alginate lyases, was purified from the culture medium of a marine bacterium, Pseudomonas sp. strain F6, to an electrophoretically homogeneous state. The enzyme was shown to have a molecular mass of 36 kDa by sodium dodecylsulfate–polyacrylamide gel electrophoresis (SDS-PAGE), and was most active at around pH 7.5 and was stable between pH 6.5 and pH 8.5. In the thermal stability experiments, the enzyme's activity diminished through an intermediate state with increasing incubation temperatures and was finally lost when heated at 100°C for 15 min. The addition of hen egg-white lysozyme to the enzyme decreased thermal stability dramatically. The apparent retention of enzyme activity (approximately 50%) was observed after the addition of 6 M guanidine hydrochloride and 8 M urea. Enzyme activity was lost completely with 10 mMSDS, while the ordered structure, which is considered likely to be β-structure, was markedly created. The similar conformational feature has also been created in marine bacterial and mollusc enzymes and the β-structure is commonly observed in polyuronate lyases. The divalent cation (Ca2+ ) promoted the activity of the calcium chelator-treated enzyme significantly, suggesting that Ca2+ is involved in the formation of the active intermediate between the acidic uronate(s) and amino acid side-chain(s) of the enzyme. 相似文献
54.
A field survey was carried out on prevalence of bovine coccidia in 9 prefectures of Japan in the autumn of 1985. A total of 1,015 fecal samples was obtained from dairy and beef cattle more than 2 weeks old. Coccidial oocysts were found in 59.0% of all the samples examined. There were no significant differences in the prevalence between dairy and beef cattle of the same age group. The prevalence was the highest in the animals between 6 and 11 months old and decreased to 25% in those more than 24 months old. In the majority of the samples positive for oocysts, OPG (the number of oocysts per gram of feces) was less than 200. No clinical coccidiosis was found except in a few cases. Eleven Eimeria and one Isospora species were identified. Of these species, E. bovis and E. ellipsoidalis were the most prevalent, followed by E. aubrunensis, E. brasiliensis, and E. cylindrica in all the prefectures. The other species identified were E. canadensis, E. alabamensis, E. zuernii, E. wyomingensis, E. bukidnonesis, E. subspherica, and Isospora sp. in the order of frequency. Isospora sp. will be a pseudoparasite caused by the contamination with feces of passerine birds. 相似文献
55.
Ovine pulmonary adenocarcinoma (OPA) is a naturally occurring retrovirus-induced transmissible lung cancer in sheep. Lungs
and associated (bronchial and mediastinal) lymph nodes of seven sheep with OPA were examined. Lungs had few multifocal consolidated
slightly elevated gray to white masses ranging from 0.5 to 3 cm in diameter. Histopathologically, these masses appeared as
well-differentiated acinar adenocarcinoma with little evidence of anaplasia. The acini composed of well-differentiated cuboidal
to low columnar epithelium with clear or vacuolated cytoplasm and low mitotic index. No metastases were observed in the bronchial
and mediastinal lymph nodes of any animal. The presence of Jaagsiekte sheep retrovirus (JSRV) was demonstrated in the lungs by immunohistochemistry. JSRV protein was detected in all tumor epithelial cells, histologically
normal alveolar type II cells, and few bronchiolar epithelial cells, alveolar macrophages, lymphocytes, and plasma cells.
This study is the first to confirm the presence of natural OPA in Egypt. 相似文献
56.
57.
Genetic male sterility (GMS) has been a useful system for the production of hybrid varieties in self-pollinated plants. We
obtained a GMS line developed from a spontaneous mutation in lettuce (Lactuca sativa L.). Genetic analysis in our previous study revealed that the sterility was controlled by a recessive gene which was named
ms-S. For simple and quick screening of individuals showing male sterility, we attempted molecular mapping of the ms-S locus using an amplified fragment length polymorphism (AFLP) technique. From the examination of 4,096 AFLP primer combinations,
63 AFLP markers were found to be linked to the gene and nine of them were successfully converted into sequence characterized
amplified region (SCAR) markers and cleaved amplified polymorphic sequence (CAPS) markers. Linkage analysis indicated that
these nine markers were closely linked to the ms-S gene and all were located on the same side of the gene. The minimum genetic distance between the ms-S gene and a marker was 3.1 cM. These results provide additional information for map-based cloning of the ms-S gene and will be of great help for lettuce breeding using GMS to produce F1 hybrids. 相似文献
58.
Masako Seki Makiko Chono Hitoshi Matsunaka Masaya Fujita Shunsuke Oda Katashi Kubo Chikako Kiribuchi-Otobe Hisayo Kojima Hidetaka Nishida Kenji Kato 《Breeding Science》2011,61(4):405-412
The genotypes of photoperiod response genes Ppd-B1 and Ppd-D1 in Japanese wheat cultivars were determined by a PCR-based method, and heading times were compared among genotypes. Most of the Japanese wheat cultivars, except those from the Hokkaido region, carried the photoperiod-insensitive allele Ppd-D1a, and heading was accelerated 10.3 days compared with the Ppd-D1b genotype. Early cultivars with Ppd-D1a may have been selected to avoid damage from preharvest rain. In the Hokkaido region, Ppd-D1a frequency was lower and heading date was late regardless of Ppd-D1 genotype, suggesting another genetic mechanism for late heading in Hokkaido cultivars. In this study, only 11 cultivars proved to carry Ppd-B1a, and all of them carried another photoperiod-insensitive allele, Ppd-D1a. The Ppd-B1a/Ppd-D1a genotype headed 6.7 days earlier than the Ppd-B1b/Ppd-D1a genotype, indicating a significant effect of Ppd-B1a in the genetic background with Ppd-D1a. Early-maturity breeding in Japan is believed to be accelerated by the introduction of the Ppd-B1a allele into medium-heading cultivars carrying Ppd-D1a. Pedigree analysis showed that Ppd-B1a in three extra-early commercial cultivars was inherited from ‘Shiroboro 21’ by early-heading Chugoku lines bred at the Chugoku Agriculture Experimental Station. 相似文献
59.
Taiko Oda Michio Namikoshi Kyoko Akano Hisayoshi Kobayashi Yoshio Honma Tadashi Kasahara 《Marine drugs》2005,3(2):64-73
Verrucarin A is an inhibitor of protein synthesis. In this study, we examined the inhibitory action of verrucarin A on signal molecules. Verrucarin A partially inhibited the IL-8 production of a PMA-stimulated promyelocytic leukemia cell line (HL-60 cells), and the effect was related to the inhibition of NF-κB activation at non-cytotoxic concentrations. Moreover, the inhibition of mitogen activated protein (MAP) kinase by verrucarin A was especially strong with p38- and JNK-phosphorylation. The findings show a new action of verrucarin A, and it is expected that this action relaxes the signal activation in response to stress. 相似文献
60.
Yasutaka Oda Kenji Tani Toshitaka Kanei Tomoya Haraguchi Kazuhito Itamoto Hiroshi Nakazawa Yasuho Taura 《Veterinary research communications》2013,37(2):133-138
Regenerative therapy using bone marrow stromal cells (BMSCs) has begun to be clinically applied in humans and dogs for neurological disorders such as spinal cord injury. Under appropriate conditions in vitro, BMSCs differentiate into neuronal cells, which may improve the effects of regenerative therapy. In this study, we evaluated canine neuron-like cells (NLCs) derived from BMSCs. We speculated on their suitability for neuro-transplantation from the point of view of their morphological features, long-term viability, abundant availability, and ability to be subcultured. Canine NLCs were differentiated as follows: third-passage BMSCs were maintained in pre-induction medium containing 2-mercaptoethanol and dimethylsulfoxide for 5 h, and then cells were transferred to neuronal induction medium containing fetal bovine serum, basic fibroblast growth factor, epidermal growth factor, dibutyryl cyclic AMP, and isobutylmethylxanthine for 7 or 14 days. Canine NLCs fulfilled the transplantation criteria and expressed markers of both immature neurons (nestin, 84.7 %) and mature neuronal cells (microtubule-associated protein-2, 95.7 %; βIII-tubulin protein, 12.9 %; glial fibrillary acidic protein, 9.2 %). These results suggest that canine BMSCs can be induced to differentiate into neuronal cells and may be suitable for neuro-transplantation. This study may provide information for improving cellular therapy for neurological diseases. 相似文献