Changes in allergenicity and digestibility of squid tropomyosin during the Maillard reaction with ribose     

Nakamura A  Sasaki F  Watanabe K  Ojima T  Ahn DH  Saeki H 《Journal of agricultural and food chemistry》2006,54(25):9529-9534

The effect of the Maillard reaction on the allergenicity of squid tropomyosin (TM) was investigated. When TM was reacted with ribose (TM-ribose), its human-specific IgE-binding ability decreased markedly and alpha-chymotryptic digestibility of TM was also altered at the early stage of the Maillard reaction. On the other hand, the modification of the lysine residues in TM using 2,4,6-trinitrobenzenesulfonic acid had no effect on the allergenicity and alpha-chymotryptic digestibility of TM. Therefore, the structural change in TM induced by the Maillard reaction would cause the reduction of the allergenicity, rather than the block of lysine residues. Although peptic digestion diminished the specific IgE-binding ability of TM, the reduction of the allergenicity by the Maillard reaction remained after peptic digestion. These results suggest that hypersensitive reaction of TM-ribose in the human body might be lower than that of native TM.  相似文献   

A rapid and simple transcriptional sequencing method for GC-rich DNA regions     

Izawa M  Kitamur N  Odake N  Maki F  Kanehira K  Nemoto H  Yamaguchi M  Yamashita A  Sasaki N  Hattori M  Kanayama S  Yoned Y 《The Japanese journal of veterinary research》2006,53(3-4):159-168

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Complications and outcomes of one-step low-profile gastrostomy devices for long-term enteral feeding in dogs and cats     

Campbell SJ  Marks SL  Yoshimoto SK  Riel DL  Fascetti AJ 《Journal of the American Animal Hospital Association》2006,42(3):197-206

Sixteen dogs and cats with a variety of primary diseases were retrospectively evaluated following endoscopic placement of a one-step low-profile gastrostomy device. Overall, the devices were well tolerated, with most complications being minor in nature. Complications included bloody or purulent peristomal discharge, peristomal swelling, peristomal inflammation, discomfort associated with the device, leaking through the device, chewing at the device, premature removal of the device, peritonitis, and aspiration pneumonia. Dogs survived for up to 2241 days, and cats survived for up to 593 days after initial device placement. The median survival time after device insertion for dogs was 89 days, and for cats it was 87 days.  相似文献   

Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and expression analysis in the mammary gland     

Yamaji D  Kimura K  Watanabe A  Kon Y  Iwanaga T  Soliman MM  Ahmed MM  Saito M 《Domestic animal endocrinology》2006,30(3):239-246

Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic cytokine that plays a crucial role in the embryonic and postnatal development of various organs including the mammary gland. We cloned bovine HGF and its c-Met receptor cDNAs, and examined their expression during mammary gland development in dairy cows. The 2.5-kbp HGF cDNA clone contained a 2190 bp open reading frame coding a 730 amino acid protein, while the 4.8-kbp c-Met cDNA clone contained a 4152 bp open reading frame coding a 1384 amino acid protein. The bovine HGF and c-Met sequences exhibited more than 87% identity with those of other mammals. RT-PCR analysis revealed ubiquitous expression of both HGF and c-Met mRNAs in various bovine tissues tested. HGF mRNA was detected only in the inactive stage of bovine mammary gland development and not in the developing, lactating, and involuting stages, while c-Met mRNA was detected in the inactive and involuting stages. Immunohistochemical analysis demonstrated that the c-Met protein was found on mammary epithelial cells in the inactive, developing, and involuting stages, and on myoepithelial cells in all stages. These results suggest pivotal roles of HGF and c-Met in the development of bovine mammary gland.  相似文献   

Discrete sandwich compounds of monolayer palladium sheets     

Murahashi T  Fujimoto M  Oka MA  Hashimoto Y  Uemura T  Tatsumi Y  Nakao Y  Ikeda A  Sakaki S  Kurosawa H 《Science (New York, N.Y.)》2006,313(5790):1104-1107

Despite the abundance of "sandwich" complexes, in which two cyclic aromatic hydrocarbon ligands flank a metal center, this motif has not been extended to sheets of multiple metal atoms. We prepared and isolated two such compounds. In the first, three palladium centers form a planar triangular array, capped by chlorides, between two cycloheptatrienyl ligands. In the second, a pentapalladium sheet adopts an edge-sharing triangle-trapezoid skeleton between two naphthacene rings. The compounds were characterized by x-ray crystallography and nuclear magnetic resonance spectroscopy. The nature of bonding in the clusters was analyzed by quantum calculations.  相似文献   

Development of EST-SSR markers of Ipomoea nil     

Tong Ly  Hiroyuki Fukuoka  Asami Otaka  Atsushi Hoshino  Shigeru Iida  Eiji Nitasaka  Nobuyoshi Watanabe  Tsutomu Kuboyama 《Breeding Science》2012,62(1):99-104

Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives.  相似文献   

A retrospective study of inflammatory colorectal polyps in miniature dachshunds     

Ohmi A  Tsukamoto A  Ohno K  Uchida K  Nishimura R  Fukushima K  Takahashi M  Nakashima K  Fujino Y  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(1):59-64

Medical records of dogs with colorectal polyps were retrospectively reviewed, and clinical presentation of inflammatory colorectal polyps in miniature dachshunds was evaluated. Of 33 dogs found to have colorectal polyps, miniature dachshunds were markedly over-represented with 16 dogs (48%), of which 12 (75%) were found to have inflammatory polyps. Multiple polyps localized between the rectum and the descending colon was the most common finding in miniature dachshunds with inflammatory polyps. Twenty dogs (80%) out of 25 miniature dachshunds with inflammatory colorectal polyps responded to immunosuppressive therapy using prednisolone and cyclosporine. The results of this study indicate that miniature dachshunds are predisposed to develop inflammatory colorectal multiple polyps, for which immunosuppressive therapy may be a treatment option.  相似文献   

Sugar expressions on the vaginal epithelium in pregnant mice     

Yasunaga Y  Takeuchi T  Shimokawa T  Nabeta M  Matsuu A  Asano A  Ohta Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(6):805-808

Sugar expressions were examined on the epithelium of both the middle portion of the vagina and the vaginal portion of the cervical canal (CC) in pregnant mice to understand the pathogenesis of bacterial infection in the female reproductive organ by using a panel of lectins. As a result, N-acetylglucosamine was positive before pregnant day (P) 7 but negative after P10 and at diestrus on both the vagina and the CC. In addition, some differences in sugar expressions were seen between them. These results suggest that sugar expressions on the mucosal surface would change not only site-specifically but also time-dependently, and these sugar differences indicate the possibility of the alteration of the settled bacterial species on the vaginal mucosa in pregnancy.  相似文献   

A case of canine multiple inflammatory colorectal polyps treated by endoscopic polypectomy and argon plasma coagulation     

Tsukamoto A  Ohno K  Irie M  Ohmi A  Maeda S  Nakashima K  Fukushima K  Fujino Y  Uchida K  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(4):503-506

Endoscopic polypectomy and argon plasma coagulation (APC) were performed in a refractory case of inflammatory colorectal polyps in a 7-year-old male Miniature Dachshund. Colonoscopic examination revealed a large sessile polyp and multiple diffuse small polyps, localized to the descending colon and rectum. The case showed a poor therapeutic response to prednisolone and cyclosporine. Under anesthesia, piecemeal resections were performed by polypectomy. APC was carried out to cauterize the polyp remnants. After treatment, reduction of the lesions and the improvement in clinical signs were observed, without recurrence of lesions for at least 10 months. Endoscopic treatment by polypectomy and APC is suggested to be a therapeutic option for refractory cases of inflammatory colorectal polyps in dogs.  相似文献   

Genetic diversity of Pinus densiflora pollen flowing over fragmented populations during a mating season     

Hajime Ozawa  Atsushi Watanabe  Kentaro Uchiyama  Yoko Saito  Yuji Ide 《Journal of Forest Research》2012,17(6):488-498

We attempted to evaluate the genetic diversity of long-distance transported pollen flowing over fragmented Pinus densiflora populations during a mating season. A P.?densiflora clonal seed orchard, which was located in a rural area where many fragmented populations exist, was selected for pollen capture. Immigrant pollen captured by three clones having different flowering times was regarded as the pollen flowing over fragmented populations during a mating season. The genetic diversity (H _e) values of the immigrant pollen captured by the three clones were high (H _e?>?0.894). The correlation of paternity (r _p) values of the seeds having immigrant parent generated from the three clones were calculated to be negative. From these parameters, the pollen cloud is considered to have maintained high genetic diversity during the mating season. The genetic composition of the pollen cloud showed slight variation. The pollen captured by different trees (i.e., clonal ramets of the three clones) was significantly different based on analysis of molecular variance. Especially, the pollen pools captured by trees planted in the western side of the orchard were significantly different from the gene pool of the surrounding populations. Factors affecting this differentiation could be that the donors of the pollen transported to the orchard vary with time, as well as nonuniform dispersal of the pollen. From these results, the pollen flowing over fragmented P.?densiflora populations is considered to have high genetic diversity, compensating to some extent for fragmentation.  相似文献