Effects of exogenous xylanase on performance,nutrient digestibility,volatile fatty acid production and digestive tract thermal profiles of broilers fed on wheat- or maize-based diet     

H.V. Masey-O’neill  M. Singh  A.J. Cowieson 《British poultry science》2014,55(3):351-359

1. A previous experiment reported that caecal temperature was negatively correlated with d 49 feed conversion ratio (FCR). This increased temperature in the caeca may indicate a prebiotic effect. An experiment was designed to investigate whether caecal temperature was affected in diets based on maize and whether other portions of the tract were affected.

2. A total of 25 Ross 308-d-old male broilers were allocated to each of 8 replicate pens per treatment. Treatments followed a 2 × 3 factorial design: two diets based on wheat or maize and three levels of enzyme addition, 0, 16 000 or 32 000 BXU/kg. Growth performance was assessed between d 1 and 49. Digestibility measurements were taken at d 28 and 49. On d 49, the excised small and large intestine of each bird was thermally imaged, weighed and volatile fatty acids (VFA) measured.

3. On d 28 and d 49, birds on the maize diets had higher feed intake and weight gain than those offered wheat diets. Additionally, on d 28, birds that received the maize diet had lower FCR than those offered the wheat diet. Enzyme improved FCR at d 49, independently of cereal. On d 28, enzyme improved the coefficient of apparent ileal DM digestibility and the coefficient of apparent ileal nitrogen digestibility. Enzyme only improved apparent ileal digestible energy in wheat-based diets (interactive term). On d 49, all digestibility parameters were improved by enzyme. Enzyme increased gizzard weight in maize-fed birds and the caeca of those fed wheat were heavier. The higher enzyme dose decreased duodenal temperature. In summary of VFA data, wheat-based diets produced more total VFAs and the total amount also increased with enzyme.

4. It appears from this study that there is equal potential in both wheat and maize diets for xylanase to improve performance of broilers probably through different mechanisms.  相似文献   

Peripheral blood leukocytes transcriptomic signature highlights the altered metabolic pathways by heat stress in zebu cattle     

Vamsikrishna Kolli  R.C. Upadhyay  Dheer Singh 《Research in veterinary science》2014

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Erratum to: Methane emissions from river buffaloes fed on green fodders in relation to the nutrient intake and digestibility     

Sonali Prusty  Madhu Mohini  Shivlal Singh Kundu  Ajay Kumar  Chander Datt 《Tropical animal health and production》2014,46(1):71-71

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Total Cystectomy for Treatment of Transitional Cell Carcinoma of the Urethra and Bladder Trigone in a Dog     

Sarah Boston DVM  DVSc  Diplomate ACVS  Ameet Singh DVM  DVSc  Diplomate ACVS 《Veterinary surgery : VS》2014,43(3):294-300

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Neurological trypanosomiasis in quinapyramine sulfate-treated horses—a breach of the blood–brain barrier?     

Muthusamy Ranjithkumar  Buddhi Chandrasekaran Saravanan  Suresh Chandra Yadav  Rajender Kumar  Rajendra Singh  Sahadeb Dey 《Tropical animal health and production》2014,46(2):371-377

Trypanosoma evansi infection typically produces wasting disease, but it can also develop into a neurological or meningoencephalitis form in equids. Trypanosomiasis in horses was treated with quinapyramine sulfate, and all the 14 infected animals were recovered clinically. After clinical recovery, four animals developed a neurological form of the disease at various intervals. Two of these animals treated with diminazene aceturate recovered temporarily. Repeated attempts failed to find the parasite in the blood or the cerebrospinal fluid (CSF), but all of the animals were positive in enzyme-linked immunosorbent assay. The calculation of the antibody index (AI) in the serum and the CSF and polymerase chain reaction (PCR) analysis of the CSF and brain tissue were carried out to confirm the neuro-infection. We found PCR and AI analyses of the CSF to be useful tools in the diagnosis of the neurological form of trypanosomiasis when the organism cannot be found in the blood or CSF. The increased albumin quotient is indicative of barrier leakage due to neuroinflammation. The biochemical changes in the CSF due to nervous system trypanosomiasis include increases in the albumin quotient, total protein, and urea nitrogen. It seems to be the first report on relapse of the nervous form of trypanosomiasis in equids even after quinapyramine treatment in endemic areas.  相似文献   

Expression of retinoid receptors in lungs of cattle,dogs, and pigs     

Shankaramurthy Channabasappa  Julia Ferguson  Baljit Singh 《Canadian journal of veterinary research》2014,78(3):176-182

Retinoids play an important role in lung development and immune response. The effects of retinoids are mediated through 2 families of retinoid receptors: retinoic acid receptors (RARs) and retinoid X receptors (RXRs), with alpha (α), beta (β), and gamma (γ) subtypes in each family. To date, no data exist on the expression pattern of retinoid receptors in lungs of cattle, dogs, and pigs. Because of the biomedical importance of retinoid receptors in inflammation and immune responses, Western blot, immunohistology, and immunoelectron microscopy were used to determine the expression of retinoid receptors in normal lungs of cattle, dogs, and pigs (n = 2 for each species). Western blot showed expression of all 6 retinoid receptor subtypes in pig lungs. Immunohistology data indicated differential expression of retinoid receptors in airway epithelium, vascular endothelium, alveolar/septal macrophages, and alveolar septum in all 3 species. Electron microscopy showed nuclear localization of retinoid receptors in neutrophils and pulmonary intravascular macrophages. Retinoic acid receptors (RAR) α subtype were localized in cytoplasmic vacuoles of pig monocytes. These data indicate constitutive expression of retinoid receptors in the lungs of cattle, dogs, and pigs.  相似文献   

Induction of immune tolerance to caseins and whey proteins by oral intubation in mouse allergy model     

U. K. Shandilya  R. Kapila  S. Singh  D. Dahiya  S. Kapila  V. K. Kansal 《Journal of animal physiology and animal nutrition》2014,98(3):467-475

This study was designed to evaluate the effect of oral tolerance of caseins (CSN) and whey proteins (WP) in alleviating the allergic response to cow's milk proteins in Swiss albino mice raised on a milk protein–free diet. Oral tolerance was induced by feeding mice with 20 mg of CSN or WP once in a day for 4 days consecutively before immunization with respective protein by intraperitoneal (i.p.) injections (20 μg 200 per μl of PBS) using 2% of alum Al(OH)₃ as adjuvant. Three weeks later, oral tolerance induction was analysed in humoral and cellular compartments of CSN‐ and WP‐fed versus saline‐fed control mice groups by measuring seric and intestinal antibody responses, mRNA abundance in splenic tissue and cytokine secretion patterns. The specific serum immunoglobulin‐E (IgE) levels were significantly suppressed (p < 0.05), while sIgA was enhanced in these groups when compared with their respective saline‐fed mice. Moreover, the mRNA levels of interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4) in both CSN‐ and WP‐tolerized mice were found to be significantly decreased, while the abundance of interleukin‐10 (IL‐10) and transforming growth factor‐β (TGF‐β) was increased significantly, as compared to respective control groups. Finally, cytokine profiles indicated a reciprocal decrease in IL‐4 and IFN‐γ versus an increase in IL‐10 secretions in supernatants of cultured splenocytes of tolerized mice. Taken together, these results clearly showed that oral administration of cows' milk caseins and whey proteins can induce significant hyposensitization in mice, with the participation of suppressor cytokines.  相似文献   

Bio-amelioration of alkali soils through agroforestry systems in central Indo-Gangetic plains of India     

Y.P. Singh  Gurbachan Singh  D.K. Sharma 《林业研究》2014,25(4):887-896

A long-term field study was initiated during 1995 at Central Soil Salinity Research Institute, Regional Research Station, Lucknow(26°47′58′′ N and 80°46′24′′ E) to analyze the effect of agroforestry systems on amelioration of alkali soils. Three agroforestry systems(pastoral, silvipastoral and silvicultural) were compared with the control where no agroforestry system was introduced. Tree-based silvicultural and silvipastoral systems were characterized by tree species Prosopis juliflora and Acacia nilotica along with grass species Leptochloa fusca, Panicum maximum, Trifolium alexandrium and Chloris gayana. Growth of ten-year-old Prosopis juliflora and Acacia nilotica planted in combination with grasses was significantly higher over the silviculture system with the same species. Tree biomass yields of P. juliflora(77.20 t?ha-1) and A. nilotica(63.20 t?ha-1) planted under silvipastoral system were significantly higher than the sole plantation of(64.50 t?ha-1 and 52.75 t?ha-1). Fodder yield under the pastoral system was significantly higher than the silvipastoral system during initial years but it was at par with that of silvipastoral systems after eight years of plantation. The microbial biomass carbon in the soils of silvipastoral systems was significantly higher than in soils under sole plantation of trees and control systems. The Prosopis-based silvipastoral system proved more effective in reducing soil pH, displacing Na+ from the exchange complex, increasing organic carbon and available N, P and K. Improvement in soil physical properties such as bulk density, porosity, soil moisture and infiltration rate was higher in the Prosopis-based silvipastoral system than in the silviculture system or control. On the basis of biomass production and improvement in soil health due to tree + grass systems, silvipastoral agroforestry system could be adopted for sustainable reclamation of highly alkali soils.  相似文献   

Growth and carcass composition of giant freshwater prawn, Macrobrachium rosenbergii (De Man), fed different isonitrogenous and isocaloric diets     

Akhil Gupta  Harjeet Singh Sehgal  & Gurpreet Kaur Sehgal 《Aquaculture Research》2007,38(13):1355-1363

A feeding experiment was conducted for 135 days to observe the effect of different isonitrogenous (35% crude protein) and isocaloric (385 kcal) diets on the growth and carcass composition of giant freshwater prawn, Macrobrachium rosenbergii ( De Man 1879 ). Three experimental diets (ED1, ED2 and ED3) were prepared using locally available ingredients. These diets differed mainly in terms of percent contribution of major protein sources such as fish meal, soybean meal, groundnut oil cake and mustard oil cake. Experimental diets were evaluated against a commercial diet, which served as the control (CD). Juveniles 1.87–2.44 g in size were stocked at a population density of 40 000 ha⁻¹ and fed thrice daily at 10% in the beginning and reducing gradually to 7% and 5% of the body weight during the experimental period. No significant differences (P>0.05) in the growth performance were observed; however, a significantly (P<0.05) higher yield (721.9 kg ha⁻¹ 135 days⁻¹) was recorded for prawn fed with control diet, followed by experimental diet 2 (676.5 kg ha⁻¹ 135 days⁻¹, having soybean meal as a major protein source). The survival ranged between 63.8% and 77.7%, with different diets showing significantly higher survival. The apparent feed conversion ratio values of diets ranged between 3.15 and 3.49, with experimental and control diets showing non‐significantly lower AFCR values. At the end of the experiment, representative specimens from each treatment were collected and their carcass composition was measured. Analysis of variance showed that carcass protein and total carbohydrate contents were significantly (P<0.05) higher in prawns fed on a fish–soybean meal‐based diet (ED3) and a control diet. The total lipid contents of prawns, however, did not differ significantly among the various dietary treatments. The results of our study suggest that the experimental diets could be used effectively for M. rosenbergii without compromising growth and flesh quality.  相似文献   

The immune response to anesthesia: Part 1     

Stacy L Anderson  Tanya Duke‐Novakovski  Baljit Singh 《Veterinary anaesthesia and analgesia》2014,41(2):113-126

ObjectiveTo review the immune response to anesthesia including mechanical ventilation, inhaled anesthetic gases, and injectable anesthetics and sedatives.Study designReview.Methods and databasesMultiple literature searches were performed using PubMed and Google Scholar from spring 2012 through fall 2013. Relevant anesthetic and immune terms were used to search databases without year published or species constraints. The online database for Veterinary Anaesthesia and Analgesia and the Journal of Veterinary Emergency and Critical Care were searched by issue starting in 2000 for relevant articles.ConclusionRecent research data indicate that commonly used volatile anesthetic agents, such as isoflurane and sevoflurane, may have a protective effect on vital organs. With the lung as the target organ, protection using an appropriate anesthetic protocol may be possible during direct pulmonary insults, including mechanical ventilation, and during systemic disease processes, such as endotoxemia, generalized sepsis, and ischemia-reperfusion injury.  相似文献