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41.
A 4‐week feeding trial was conducted to test the use of marine yeasts purified from seawater and sediments as a dietary source in cultivating a cladoceran, Moina macrocopa, a potential alternative live food for fish larvae. Optimal growth conditions of two yeast strains were obtained for NaCl concentration, pH and temperature. Moina macrocopa was cultivated using different diets: marine yeasts (Debaryomyces hansenii Yeast‐14 and Candida austromarina Yeast‐16) and a commercial diet (Erythrobacter sp. Sπ‐I). The essential amino acids of both the yeast strains were nearly as great as those in M. microcopa. Further, the yeast‐fed M. macrocopa had essential amino acid profiles similar to the documented values for rotifers and Artemia enriched in microalgae and commercial diets. Erythrobacter sp. Sπ‐I lacked n‐3 polyunsaturated fatty acids, 20:5n‐3 and 22:6n‐3, which were also low but detected in both yeasts. An increase in the 20:5n‐3 [eicosapentaenoic acid (EPA)] and 22:6n‐3 [docosahexaenoic acid (DHA)] levels, compared with the levels in yeast strains themselves, was more pronounced in the 22:6n‐3 level of Moina fed the C. austromarina, resulting in a high DHA:EPA ratio. When the Moina diets were switched, their δ13C values shifted gradually towards the values of the switched diets. Diet switch from Erythrobacter sp. Sπ‐I to C. austromarina Yeast‐16 resulted in a more rapid turnover of Moina tissue carbon than that in the inverse case. When fed a mixed diet, the δ13C values of Moina tissue approached the value of marine yeasts immediately. These temporal changes in the δ13C values of Moina tissue indicate the preferential ingestion of marine yeasts and a selective assimilation of the carbon originated from marine yeasts. These findings suggest that marine yeasts, particularly C. austromarina Yeast‐16, are highly available to mass cultures of M. macrocopa, providing better nutritional and dietary values than the commercial diet (Erythrobacter sp. Sπ‐I).  相似文献   
42.
To evaluate the performance of new rice establishment methods viz., system of rice intensification (SRI) and integrated crop management (ICM), a field study was conducted during 2008–11 in South Garo Hills, Meghalaya, foot hills of Eastern Himalayas, India. Field demonstrations were undertaken during wet seasons of 2008–11 and socio-economic information in the context of farmer’s realities were obtained during 2010–11 using a well structured questionnaire administered to 134 farmers. The results indicated that the average higher productivity of rice under SRI and ICM demonstration was 209.9 and 185.4 %, respectively, over conventional rice culture (CRC). The SRI and ICM methods of rice cultivation could save seeds (97.56 and 60.98 %), saving water (78.05 and 63.66 %), reduce cost (70.33 %), higher yield etc. compared to CRC. The main reasons for non-adoption of SRI/ICM was related to involvement of more efforts, faith towards traditional practices, ignorance and lack of knowledge on scientific water management. The net-return of $816.69, $706.63 and $51.48/ha was realized under SRI, ICM and CRC, respectively. The co-efficient of multiple determinations (R 2) of the production function was 0.695 in SRI, 0.714 in ICM and 0.734 in CRC which indicated that about 69.5, 71.4 and 73.4 % of the variation in rice productivity under SRI, ICM and CRC, respectively were explained by the independent variable and remaining 30.5, 28.6 and 26.6 %., respectively in SRI, ICM and CRC were as a result of non-inclusion of some explanatory variables as well as other factors outside the farmers control.  相似文献   
43.
Poly(ethylene terephthalate) (PET)/CaCO3 and PET/modified-CaCO3 (m-CaCO3) nanocomposites were prepared by melt blending. The morphology indicated that m-CaCO3 produced by reacting sodium oxalate and calcium chloride, was well dispersed in PET matrix and showed good interfacial interaction with PET compared to CaCO3. No significant differences in the thermal properties such as, glass transition, melting and degradation temperatures, of the nanocomposites were observed. The thermal shrinkage of PET at 120 °C was 10.8 %, while those of PET/CaCO3 and PET/m-CaCO3 nanocomposites were 2.9–5.2 % and 1.2–2.8 %, respectively depending on filler content. The tensile strength of PET/CaCO3 nanocomposite decreased with CaCO3 loading, whereas that of PET/m-CaCO3 nanocomposites at 0.5 wt% loading showed a 17 % improvement as compared to neat PET. The storage modulus at 120 °C increased from 1660 MPa for PET to 2350 MPa for PET/CaCO3 nanocomposite at 3 wt% loading, and 3230 MPa for PET/m-CaCO3 nanocomposite at 1 wt% loading.  相似文献   
44.
The present study examined the possibility of long‐term storage, by cryopreservation in liquid nitrogen, of the sperm of filefish (Thamnaconus septentrionalis). Changes in motility, survival rate, ultrastructure and fertilization rate of the sperm after freezing and thawing were tested. For selection of the immobilizing solution, artificial seawater (ASW) of 250, 350 and 450 mOsmol kg?1 were tested. Sperm motility was significantly inhibited in 350 mOsmol kg?1 ASW, and restored entirely after 100% ASW (1200 mOsmol kg?1) was added. Two cryoprotectants, dimethyl sulphoxide and glycerol, were employed. The sperm was diluted at the ratio of 1:6 with the extenders, and frozen at a freezing rate of ?40°C min?1 to ?100°C after equilibration for 10 min at room temperature, followed by plunging into liquid nitrogen. The highest post‐thawed sperm motility and survival rate were obtained with 5% glycerol. Afterwards, the effect of different freezing rates was examined using 5% glycerol as a cryoprotectant, and the rate of ?30°C min?1 to ?100°C showed the best result.  相似文献   
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Heartworm disease caused by Dirofilaria immitis affects canine and feline hosts, with infections occasionally being reported in humans. Studies have shown that both dirofilarial antigens and those derived from its bacterial endosymbiont Wolbachia, interact with the host organism during canine, feline and human infections and participate in the development of the pathology and in the regulation of the host’s immune response. Both innate and acquired immune responses are observed and the development of the acquired response may depend on the host and, or on its parasitological status. This review aims at illustrating current research on the role of both D. immitis and Wolbachia, in the immunology and immunopathology of dirofilariosis.  相似文献   
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49.
It would be preferable to use a reliable crop growth model for studies on climate change impact assessment. The objectives of this study was to evaluate simulation performance for two maize models, including CERES-Maize and IXIM models, included in the DSSAT model (version 4.6) in terms of phenology and yield. Two early maturing cultivars, Chalok#1 and Junda# 6, were grown under controlled environment in plastic houses at Suwon, Korea. Each cultivar, which was sown at four different date in 2013 and 2014, was subjected to four sets of temperature conditions including ambient (AT), AT+1.5°C, AT+3°C, and AT+5°C. In simulations of phenology under given conditions, the anthesis date and grain filling ratio were underestimated, especially when temperature was unusually high, e.g., in 2013. The maize models also had poor accuracy in grain yield, which resulted from the fact that these models had relatively large errors in simulation of kernel number and kernel weight under elevated temperature conditions. In addition, both models were not able to simulate the drastic decrease of kernel number due to heat stress around flowering periods. These results indicated that two maize models would need improvements in simulation of crop response to supra-optimal temperature before they would be used to assess the impact of the climate change on maize yield. This studies merits further study to improve algorithms in phenology simulation at supraoptimal temperature.  相似文献   
50.
Data from a monitoring program on precipitation at four sites in South Korea during the period from 1993 to 1998 were analyzed for determining a time trend relationship in chemical composition of precipitation. The concentrations of nss-sulphate, ammonium and calcium were found to have decreased over time at a statistically significant level in Seoul (p<0.05). The concentration of nss-sulphate had declined by a rate of 12% per year for the period from 1993 to 1998. The concentrations of ammonium and calcium decreased by 11% and 12% per year respectively for the same period. The concurrent reduction of cation offsets the change in strong acid anion, thus the annual mean pH of precipitation has been approximately constant. In contrast to the rapid change of sulphate, the second most abundant anion, nitrate, showed no statistically significant decrease. As a result, the nitrate to nss-sulphate ratio (N/S ratio) has increased rapidly; the N/S ratio in precipitation has increased from less than 0.1 in 1985 to nearly 0.5 in 1998 at Seoul. In spite of the short monitoring period, a significant upward trend in the N/S ratio was also observed at all of the other sites suggesting that the nitrogen species contribute more to precipitation acidity throughout Korea.  相似文献   
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