Relationships between clinical signs, pathological changes and tissue distribution of Mycobacterium avium subspecies paratuberculosis in 21 cows from herds affected by Johne's disease   总被引：1，自引：0，他引：1  

Brady C  O'Grady D  O'Meara F  Egan J  Bassett H 《The Veterinary record》2008,162(5):147-152

Twenty-one cows from eight herds affected by Johne's disease were assigned to four groups: seven were not thriving and had persistent diarrhoea, six were not thriving and had intermittent diarrhoea, four were not thriving but did not have diarrhoea, and four were clinically normal. Postmortem, macroscopic lesions consistent with Johne's disease were identified in 17 of the cows and Mycobacterium avium subspecies paratuberculosis (MAP) was isolated from all of them. However, except for the fact that diarrhoea was correlated with the presence of lesions in the large intestine there was little correlation between the presence or absence of clinical signs and the lesions associated with Johne's disease. The tissue distribution of MAP was also poorly correlated with either the clinical signs or the lesions. The organism was widely distributed in 17 of the 21 cows, including three of the clinically normal animals, and was present in the mammary tissues of seven cows including two of the clinically normal animals. Three distinct histopathological patterns were observed in the affected intestines: infiltration of the lamina propria with giant cells, tuberculoid lesions, and lepromatous lesions; the lepromatous lesions were associated with extensive pathological changes.  相似文献   

Effects of dietary cation-anion balance on blood PH, acid base parameters, serum and urine mineral levels, and parathyroidhormone (PTH) in weanling horses.     

S. R. Cooper  K. H. Kline  J. H. Foreman  H. A. Brady  C. E Shipley  L. P. Frey  K. A. Sennello 《Journal of Equine Veterinary Science》1995,15(10)

This study demonstrated that the feeding of treatment diets with calculated dietary cation-anion balances (DCAB) of +370.43 (H) and -25.69 (L) did not have significant effects on blood pH, pCO₂, and HCO₃-. Serum Ca²⁺, P, Na⁺, and Cl^- as well as plasma PTH did not differ (P > .05) between the two treatment groups. Serum K⁺ was higher (P< .05) in horses fed diet H rather than diet L. The DCAB of the diet significantly affected urinary Ca²⁺, P, Na⁺, K⁺, and Cl^- excretion in the young growing horse. Urine Ca²⁺ and Cl- levels were higher (P < .01) in horses fed diet H versus diet L. Furthermore, levels of P, Na⁺, and K⁺ in the urine were higher (P < .01) in horses on diet H as opposed to diet L. Results of this study indicate that horses were able to maintain acid-base status regardless of diet. However, these data imply that growing horses consuming diets low in DCAB may be predisposed to abnormal bone mineralization due to the increase in calcium excretion which could lead to a weakening of the skeletal system.  相似文献   

Comparison of unilateral versus bilateral nasal catheters for oxygen administration in dogs     

Elizabeth D. Dunphy  DVM    F. A. Mann  DVM  MS  DACVS  DACVECC    John R. Dodam  DVM  MS  PhD  DACVA    Keith R. Branson  DVM  MS  DACVA    Colette C. Wagner-Mann  DVM  PhD    Paula A. Johnson  DVM    Mark A. Brady  DVM 《Journal of Veterinary Emergency and Critical Care》2002,12(4):245-251

Objective: To determine the effect of bilateral nasal oxygen supplementation on tracheal airway and arterial blood gas parameters. Design: Original research. Setting: Research Laboratory. Animals: Eight normal dogs. Interventions: None. Measurements: Intra‐tracheal oxygen concentration and arterial oxygen partial pressure at three different oxygen flow rates given through either unilateral or bilateral nasal catheters. Main results: F_IO₂ and PaO₂ were significantly increased with higher total oxygen flow rates, but the increase was the same whether the higher flow was delivered through one nasal catheter or divided and administered though two nasal catheters. The use of bilateral nasal catheters allowed a tracheal F_IO₂ as high as 0.60 with minimal patient discomfort. Conclusions: The benefit of bilateral nasal catheters for oxygen supplementation is the ability to provide high total oxygen flows with decreased risk of patient discomfort. If the desired oxygen flow can be achieved with a unilateral nasal catheter, then the only benefit of bilateral catheters is increased patient comfort. The use of bilateral nasal oxygen catheters for oxygen supplementation can result in an F_IO₂ that is high enough to produce oxygen toxicity with prolonged administration.  相似文献   

Local and systemic complications in a cat after poorly performed dental extractions     

Reiter AM  Brady CA  Harvey CE 《Journal of veterinary dentistry》2004,21(4):215-221

This case report describes a cat that was presented with infected oral tissues and signs of systemic inflammatory response. Incomplete dental extractions had been performed 10-days earlier During a second dental procedure, 22 tooth root remnants were removed. Diabetic ketoacidosis and possible thromboembolism complicated the cat's recovery period After 13-days of hospitalization, the patient was stable enough to be sent home. Postoperative examinations at 1, 3, 6, 12, 16, 24, and 52-weeks indicated continued improvement with moderate glycemic control and chronic but stable renal failure. The patient died from further complications of diabetic ketoacidosis 20-months following root remnant extractions.  相似文献   

Severe sepsis in cats: 29 cases (1986-1998)     

Brady CA  Otto CM  Van Winkle TJ  King LG 《Journal of the American Veterinary Medical Association》2000,217(4):531-535

OBJECTIVE: To document the clinical, clinicopathologic, and pathologic findings in cats with severe sepsis, identify abnormalities unique to this species, and identify criteria that could be used antemortem to diagnose the systemic inflammatory response syndrome in cats. DESIGN: Retrospective study. ANIMALS: 29 cats confirmed to have severe sepsis at necropsy. PROCEDURE: Pertinent history, physical examination findings, and results of hematologic and biochemical testing were extracted from medical records. RESULTS: Clinical diagnoses included pyothorax, septic peritonitis, bacteremia secondary to gastrointestinal tract disease, pneumonia, endocarditis, pyelonephritis, osteomyelitis, pyometra, and bite wounds. Physical examination findings included lethargy, pale mucous membranes, poor pulse quality, tachypnea, hypo- or hyperthermia, signs of diffuse pain on abdominal palpation, bradycardia, and icterus. Clinicopathologic abnormalities included anemia, thrombocytopenia, band neutrophilia, hypoalbuminemia, low serum alkaline phosphatase activity, and hyperbilirubinemia. Necropsy findings included multi-organ necrosis or inflammation with intralesional bacteria. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that severe sepsis in cats is characterized by lethargy, pale mucous membranes, signs of diffuse abdominal pain, tachypnea, bradycardia, weak pulses, anemia, hypoalbuminemia, hypothermia, and icterus. Recognition of this combination of clinical findings should facilitate the diagnosis of severe sepsis in cats.  相似文献   

Thyroid gangliosides with high affinity for thyrotropin: potential role in thyroid regulation     

Mullin BR  Pacuszka T  Lee G  Kohn LD  Brady RO  Fishman PH 《Science (New York, N.Y.)》1978,199(4324):77-79

Thyroid cell membranes contain a multiplicity of gangliosides, some of which inhibit thyrotropin binding to thyroid membranes. The most potent inhibitor is a ganglioside which is present in only trace amounts and appears to have a novel structure. Thyroid gangliosides may play a role in relaying the hormonal message to the thyroid cell.  相似文献   

Metachromatic leukodystrophy: diagnosis with samples of venous blood   总被引：14，自引：0，他引：14  

A K Percy  R O Brady 《Science (New York, N.Y.)》1968,161(841):594-595

Arylsulfatase A and B have been demonstrated in preparations of human leukocytes. The level of activity of arylsulfatase A is markedly decreased in the preparations from patients with metachromatic leukodystrophy. Acid phosphatase and arylsulfatase B activities were normal. The assay of arylsulfatase A in leukocyte preparations can be useful in the diagnosis of metachromatic leukodystrophy while obviating the difficulties of current methods.  相似文献   

Fast axonal transport in squid giant axon   总被引：35，自引：0，他引：35  

R D Allen  J Metuzals  I Tasaki  S T Brady  S P Gilbert 《Science (New York, N.Y.)》1982,218(4577):1127-1129

Video-enhanced contrast-differential interference contrast microscopy has revealed new features of axonal transport in the giant axon of the squid, where no movement had been detected previously by conventional microscopy. The newly discovered dominant feature is vast numbers of "submicroscopic" particles, probably 30- to 50-nanometer vesicles and other tubulovesicular elements, moving parallel to linear elements, primarily in the orthograde direction but also in a retrograde direction, at a range of steady velocities up to +/- 5 micrometers per second. Medium (0.2 to 0.6 micrometer) and large (0.8 micrometer) particles move more slowly and more intermittently with a tendency at times to exhibit elastic recoil. The behavior of the smallest particles and the larger particles during actual translocation suggests that the fundamental processes in the mechanisms of organelle movement in axonal transport are not saltatory but continuous.  相似文献   

Application of the TRAP technique to lettuce (<Emphasis Type="Italic">Lactuca sativa</Emphasis> L<Emphasis Type="Italic">.</Emphasis>) genotyping     

Jinguo?HuEmail author  Oswaldo?E.?Ochoa  María?José?Truco  Brady?A.?Vick 《Euphytica》2005,144(3):225-235

Summary To demonstrate the applicability of the target region amplification polymorphism (TRAP) marker technique to lettuce genotyping, we fingerprinted 53 lettuce (Lactuca sativa L.) cultivars and six wild accessions (three from each of the two wild species, L. saligna L. and L. serriola L.). Seven hundred and sixty-nine fragments from 50 to 900 bp in length were amplified in 10 PCR reactions using 10 fixed primers in combination with four fluorescent labeled arbitrary primers. Three hundred and eighty-eight of these fragments were polymorphic among the 59 Lactuca entries and 107 fragments were polymorphic among the 53 lettuce cultivars and the six wild accessions; 251 fragments were present only in the wild species. These markers not only discriminated all cultivars, but also revealed the evolutionary relationship among the three species: L. sativa, the cultivated species, is more closely related to L. serriola than to L. saligna. Cluster analysis grouped the cultivars by horticultural types with a few exceptions. These results are consistent with previous findings using RFLP, AFLP, and SAMPL markers. The TRAP markers revealed significant differences in genetic variability among horticultural types, measured by the average genetic similarity among the cultivars of the same type. Within the sample set, the leaf type and butterhead types possessed relatively high genetic variability, the iceberg types had moderate variability and the romaine types had the lowest variability. The genetic behavior of TRAP markers was assessed with a mapping population of 45 recombinant inbred lines (RILs) derived from an interspecific cross between L. serriola and L. sativa. Almost all the markers segregated in the expected 1:1 Mendelian ratio and are being incorporated into the existing lettuce linkage maps. Our results indicate that the TRAP markers can provide a powerful technique for fingerprinting lettuce cultivars. The U.S. Government's right to retain a non-exclusive, royalty-free license in and to any copyright is acknowledged.  相似文献   

Survival time and relapse in dogs with meningoencephalomyelitis of unknown origin treated with prednisolone and ciclosporin: a retrospective study     

SL Brady  AP Woodward  MAR le Chevoir 《Australian veterinary journal》2020,98(10):491-498

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