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A genome scan to detect QTL influencing growth and carcass-related traits was conducted in a Charolais x Holstein crossbred cattle population. Phenotypic measurements related to growth and carcass traits were made on the 235 second-generation crossbred males of this herd (F2 and reciprocal backcrosses), which were born in 4 consecutive annual cohorts. Traits measured in vivo were related to birth dimensions, growth rates, and ultrasound measurements of fat and muscle depth. The animals were slaughtered near a target BW of 550 kg, and a wide range of postmortem traits were measured: visual assessment of carcass conformation and carcass fatness, estimated subcutaneous fat percentage, weights of kidney knob and channel fat, and weights of carcass components after commercial and full-tissue dissections. The whole population, including grandparents, parents, and the crossbred bulls, was genotyped initially for 139 genome-wide microsatellite markers. Twenty-six additional markers were subsequently analyzed to increase marker density on some of the chromosomes where QTL had been initially identified. The linear regression analyses based on the 165 markers revealed a total of 51 significant QTL at the suggestive level, 21 of which were highly significant (F-value >or=9; based on the genome-wide thresholds obtained in the initial scan). A large proportion of the highly significant associations were found on chromosomes 5 and 6. The most highly significant QTL was localized between markers DIK1054 and DIK082 on chromosome 6 and explained about 20% of the phenotypic variance for the total bone proportion estimated after the commercial dissection. In the adjacent marker interval on this chromosome, 2 other highly significant QTL were found that explain about 30% of the phenotypic variance for birth dimension traits (BW and body length at birth). On chromosome 5, the most significant association influenced the lean:bone ratio at the forerib joint and was flanked by markers DIK4782 and BR2936. Other highly significant associations were detected on chromosomes 10 (estimated subcutaneous fat percentage), 11 (total saleable meat proportion), 16 (prehousing growth rate), and 22 (bone proportion at the leg joint). These results provide a useful starting point for the identification of the genes associated with traits of direct interest to the beef industry, using fine mapping or positional candidate gene approaches.  相似文献   
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Background: The platelet function analyzer (PFA)‐100 is a point‐of‐care instrument previously evaluated in humans and dogs. In both species, artificially prolonged platelet closure time (CT) occurs with anemia. Reliability of the analyzer in dogs becomes a concern when the HCT is between 0.25 and 0.35 L/L. Objective: The objective of this study was to further define the level of HCT at which CT is prolonged, using in vitro diluted canine blood. Methods: Citrated whole blood samples were collected from 22 healthy dogs. Initial HCT was determined and autologous platelet‐rich plasma was added to samples to achieve HCTs of 0.33, 0.30, and 0.27 L/L. CT was determined in duplicate on the PFA‐100 using collagen/adenosine‐5′‐diphosphate cartridges. Results: Compared with the initial CT in samples with HCT 0.39–0.54 L/L (CT mean±SD=57.8±5.75 seconds), significantly prolonged CTs were found in hemodiluted samples with HCT 0.33 L/L (61.1±4.64 seconds), 0.30 L/L (64.3±6.79 seconds), and 0.27 L/L (70.8±7.90 seconds) (P=0.029; repeated measures ANOVA). Conclusion: Although statistical differences were found, further studies are needed to determine the clinical significance of the mild prolongation in CT associated with mild anemia. Until then, dogs with HCTs slightly <0.35 L/L should be evaluated cautiously for platelet dysfunction using the PFA‐100.  相似文献   
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This study described a scoring system for the assessment of apparent neck adiposity and evaluated morphometric measurements for assessment of neck and overall adiposity. Twenty-one barren Thoroughbred mares, 13 Arabian geldings and 75 Welsh, Dartmoor, or crossbred pony mares, were clinically examined and blood samples analysed for insulin, glucose, leptin, and triglycerides. Bodyweight (BW), height, length, girth and abdominal circumferences, neck length, neck crest height and neck circumference were measured, and body condition scores (BCS) and cresty neck scores (CNS) were rated. Girth:height ratio had the strongest associations with BCS (r(s)=0.64, P<0.001 in horses; r(s)=0.83, P<0.001 in ponies) and blood variables, such as leptin (r(s)=0.39, P=0.024 in horses; r(s)=0.68, P<0.001 in ponies). Crest height and neck circumference:height ratio had the strongest association with CNS (r(s)>0.50, P<0.01) and blood variables, such as insulin (r(s)0.40, P<0.05). Cresty neck score was useful in the assessment of neck crest adiposity and had physiological relevance, as demonstrated by associations with blood variables. Girth:height was the most suitable morphometric for assessment of overall adiposity, and either crest height or neck circumference:height was a suitable morphometric for assessment of apparent neck adiposity.  相似文献   
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In order to restore biodiversity in the degraded forest landscape and to use forest plantations for climate change mitigation, experimental plantations of indigenous trees (including mahogany species) and important exotic trees species like Tectona grandis have been established in pure and in mixed stands in the degraded Tain Tributaries Block II Forest Reserve in the dry semi-deciduous forest zone of Ghana. This study assessed the performance of an important indigenous species, Khaya grandifoliola, in pure and in mixed stands, and compared its performance to the exotic tree species, T. grandis. The results from the study indicated that after 4 years, there was a significant difference in the diameter of K. grandifoliola (P = 0.001) between pure and mixed stands with the pure stands having an average diameter of 9.15 ± 0.19 cm compared with 7.81 ± 0.33 cm for mixed stands. Pure stands had a correspondingly higher basal area at breast height for individual trees in pure stands compared with mixed stands. K. grandifoliola also recorded average total height of 5.50 ± 0.13 m and merchantable height 3.63 ± 0.09 m in pure stands, compared to total height of 5.04 ± 0.24 m and merchantable height of 3.52 ± 0.18 m in mixed stands. However, these values were not significantly different between the stands (P > 0.05). Basal area at breast height for K. grandifoliola grown in pure stands was 5.5 ± 0.3 m2/ha at age four, which was significantly higher than the basal area of 1.1 ± 0.4 m2/ha at breast height for K. grandifoliola in mixed stands. Also total volume per hectare was higher in pure stands (17.8 ± 0.9 m3/ha) than in mixed stands (3.4 ± 0.6 m3/ha). Consequently, K. grandifoliola accumulated more carbon in pure stands (10,126 ± 557.2 kg/ha) than in mixed stands (1,976 ± 335.1 kg/ha), but the mixture of the tree species, including K. grandifoliola, accumulated more carbon (11,929 ± 401.3 kg/ha) than the K. grandifoliola in pure stands though not statistically different. Overall, K. grandifoliola performed better in pure stands than in mixed stands. With regards to the tolerance to pest attacks on K. grandifoliola, it was more tolerant to pests’ attacks in mixed stands than in pure stands. There was no statistical difference in diameter growth between K. grandifoliola (9.15 ± 0.19 cm) and T. grandis (9.61 ± 0.26 cm) in pure stands. The values of total height, merchantable height and total volume for T. grandis were 8.22 ± 0.20 m, 5.38 ± 0.18 m and 22.5 ± 1.7 m3/ha respectively which differed significantly from 5.50 ± 0.13 m, 3.63 ± 0.09 m and 17.8 ± 0.9 m3/ha for K. grandifoliola for the same parameters (P < 0.05). However, there was no significant difference between the two species with respect to basal area per hectare (P = 0.189); K. grandifoliola grew to 5.5 ± 0.3 m2/ha and T. grandis grew to 4.8 ± 0.3 m2/ha. T. grandis in pure stands accumulated slightly more aboveground biomass than K. grandifoliola in pure stands after 4 years though not statistically different.  相似文献   
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