全文获取类型
收费全文 | 397篇 |
免费 | 56篇 |
专业分类
林业 | 16篇 |
农学 | 6篇 |
基础科学 | 10篇 |
42篇 | |
综合类 | 58篇 |
农作物 | 42篇 |
水产渔业 | 12篇 |
畜牧兽医 | 258篇 |
园艺 | 7篇 |
植物保护 | 2篇 |
出版年
2020年 | 4篇 |
2019年 | 6篇 |
2018年 | 9篇 |
2017年 | 6篇 |
2016年 | 12篇 |
2015年 | 9篇 |
2014年 | 19篇 |
2013年 | 25篇 |
2012年 | 20篇 |
2011年 | 12篇 |
2010年 | 13篇 |
2009年 | 8篇 |
2008年 | 30篇 |
2007年 | 16篇 |
2006年 | 15篇 |
2005年 | 16篇 |
2004年 | 12篇 |
2003年 | 13篇 |
2002年 | 9篇 |
2001年 | 8篇 |
2000年 | 19篇 |
1999年 | 6篇 |
1998年 | 3篇 |
1997年 | 12篇 |
1996年 | 8篇 |
1995年 | 4篇 |
1994年 | 12篇 |
1993年 | 4篇 |
1992年 | 6篇 |
1991年 | 8篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 9篇 |
1986年 | 5篇 |
1984年 | 3篇 |
1981年 | 3篇 |
1979年 | 4篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1975年 | 6篇 |
1974年 | 4篇 |
1971年 | 3篇 |
1970年 | 8篇 |
1968年 | 3篇 |
1966年 | 3篇 |
1963年 | 4篇 |
1955年 | 2篇 |
1952年 | 2篇 |
1948年 | 2篇 |
1947年 | 2篇 |
排序方式: 共有453条查询结果,搜索用时 15 毫秒
51.
52.
CASE HISTORY: A 14-year-old neutered male Bearded Collie was presented with a history of recurrent, intermittent urinary incontinence of 7 years duration. CLINICAL FINDINGS: A large, firm, non-painful mass was found in the mid-abdominal region on palpation. Ultrasonography of the mass revealed a compartmentalised structure with mixed echogenicity, and which did not appear to be associated with any of the abdominal organs. Ultrasound-guided fine needle aspirates contained several clusters of epithelial cells with cytological features of hepatocytes. At exploratory laparotomy, the mass was found in the gastrosplenic ligament within the greater omentum. PATHOLOGICAL FINDINGS AND DIAGNOSIS: Histopathologically, the mass consisted of sheets of hepatocytes, but without the characteristic hepatic architecture. The cells showed moderate variation in nuclear size and were sometimes binucle- ate. A diagnosis of hepatocellular carcinoma (HCC) in the mesentery was made. CLINICAL RELEVANCE: The presence of ectopic hepatic tissue has been reported rarely in man and cats, but not in the dog. Neoplastic transformation of ectopic hepatic tissue is seen in man. This is the first report of the presentation, clinical findings and treatment of a dog with ectopic HCC. 相似文献
53.
Gaseous and leaching nitrogen losses from no-tillage and conventional tillage systems following surface application of cattle manure 总被引:2,自引:0,他引:2
M.S. Mkhabela A. Madani R. Gordon D. Burton D. Cudmore A. Elmi W. Hart 《Soil & Tillage Research》2008,98(2):187-199
Previous studies have demonstrated inconsistent results on the impact of tillage systems on nitrogen (N) losses from field-applied manure. This study assessed the impact of no-tillage (NT) and conventional tillage (CT) systems on gaseous N losses, N2O:N2O + N2 ratios and NO3−-N leaching following surface application of cattle manure. The study was undertaken during the 2003/2004 and 2004/2005 seasons at two field sites in Nova Scotia namely, Streets Ridge (SR) in Cumberland County and the Bio-environmental Engineering Centre (BEEC) in Truro. Results showed that the NT system had higher (p < 0.05) NH3 losses than CT. Over the two seasons, manure incorporation in CT reduced NH3 losses on average by 86% at SR and 78% at BEEC relative to NT. At both sites and during both seasons, denitrification rates and N2O fluxes in NT were generally higher than in CT plots, presumably due to higher soil water and organic matter content in NT. Over the two seasons, mean denitrification rates at SR were 239 and 119 g N ha−1 d−1, while N2O fluxes were 120 and 64 g N ha−1 d−1 under NT and CT, respectively. At BEEC mean denitrification rates were 114 and 71 g N ha−1 d−1, while N2O fluxes were 52 and 27 g N ha−1 d−1 under NT and CT, respectively. Conversely, N2O:N2O + N2 ratios were lower in NT than CT suggesting more complete reduction of N2O to N2 under NT. When averaged across all soil depths, NO3−-N was higher (p < 0.05) in CT than NT. Nitrate-N decreased with depth at both sites regardless of tillage. In most cases, NO3−-N was higher under CT than NT at all soil depths. Similarly, flow-weighted average NO3−-N concentrations in drainage water were generally higher under CT. This may be partly attributed to higher denitrification rates under NT. Therefore, NT may be a viable strategy to remove NO3−-N from the soil, and thus, reduce NO3−-N contamination of groundwater. However, it should be noted that while the use of NT reduces NO3−-N leaching it may come with unintended environmental tradeoffs, including increased NH3 and N2O emissions. 相似文献
54.
55.
Relationships among milk urea-nitrogen, dietary parameters, and fecal nitrogen in commercial dairy herds 下载免费PDF全文
Pipat Arunvipas John A. VanLeeuwen Ian R. Dohoo Greg P. Keefe Shelley A. Burton Kerry D. Lissemore 《Canadian journal of veterinary research》2008,72(5):449-453
Our study objective was to determine the ability of milk urea-nitrogen concentrations ([MUN]) to predict fecal nitrogen concentrations ([Fecal N]) in commercial dairy herds. A total of 83 dairy herds were each visited 3 times within 48 h after a monthly herd milk test. For each farm visit, forages were sampled for nutrient analyses, which were entered into a computerized ration evaluator, and fecal samples were taken per rectum from each of 6 cows (2 early-, 2 mid-, and 2 late-lactation). Fecal samples were pooled, mixed, and analyzed for nitrogen content. Fecal nitrogen concentrations were compared with the routinely measured nutritional parameters from the ration evaluation, and the herd average [MUN] for the previous milk test date using mixed linear regression analyses. Total protein supplied in the ration was significantly positively associated with [Fecal N], but herd average [MUN] was not associated (P > 0.10) with [Fecal N]. 相似文献
56.
Buckham Sporer KR Weber PS Burton JL Earley B Crowe MA 《Journal of animal science》2008,86(6):1325-1334
Transportation causes stress in cattle that may alter numerous physiological variables with a negative impact on production and health. The objectives of the current study were to investigate the physiological effects of truck transportation and to characterize a pattern of phenotypes in the circulation that may aid in the early identification of stress-susceptible animals that often succumb to severe respiratory disease. Thirty-six young beef bulls (Aberdeen Angus, n = 12; Friesian, n = 12; and Belgian Blue x Friesian, n = 12) were subjected to a 9-h truck transportation by road. Blood (10 mL) was collected at -24, 0, 4.5, 9.75, 14.25, 24, and 48 h relative to the initiation of transportation (0 h). Plasma was collected for the assay of various metabolic, inflammatory, and steroid variables, and total leukocyte counts were determined in whole blood at each time point. Body weight and rectal temperature were recorded at -24, 9.75, and 48 h. Transportation decreased measures of protein metabolism in the plasma, including albumin (P = 0.002), globulin (P < 0.001), urea (P = 0.006), and total protein (P < 0.001), and increased creatine kinase (P < 0.001). The energy substrate beta-hydroxybutyrate was not changed (P = 0.27). Acute phase proteins haptoglobin and fibrinogen were both decreased (P < 0.001), whereas total leukocyte counts were elevated (P = 0.002). Circulating steroid concentrations were altered, because a classical acute increase in plasma cortisol was observed with the onset of transit (P < 0.001), in association with a decrease in dehydroepiandrosterone (P = 0.07), resulting in a profound increase in cortisol:dehydroepiandrosterone ratio (P < 0.001). Plasma testosterone was decreased, whereas plasma progesterone was increased (P < 0.001) in association with the increase in cortisol (P < 0.001). There was also an effect of breed for all variables except plasma urea, creatine kinase, and testosterone, perhaps indicating that a genetic component contributed to the physiological response to transportation stress, although without any clear trend. Taken together, this profile of physiological variables in the circulation of transportation-stressed bulls may aid in the future detection of disease-susceptible cattle after transportation. Further research to validate these potential biomarkers is necessary. 相似文献
57.
Sita S. Withers Daniel York Jin W. Choi Kevin D. Woolard Renee Laufer‐Amorim Ellen E. Sparger Jenna H. Burton Stephen J. McSorley Arta M. Monjazeb William J. Murphy Robert J. Canter Robert B. Rebhun 《Veterinary and comparative oncology》2019,17(3):242-252
Our lack of understanding of the immune microenvironment in canine osteosarcoma (cOSA) has limited the identification of potential immunotherapeutic targets. In particular, our ability to utilize readily available tissue from a dog's primary tumour to predict the type and extent of immune response in their pulmonary metastatic lesions is unknown. We, therefore, collected 21 matched pairs of primary tumours and pulmonary metastatic lesions from dogs with OSA and performed immunohistochemistry to quantify T‐lymphocyte (CD3), FOXP3+ cell, B‐lymphocyte (Pax‐5), and CD204+ macrophage infiltration. We found that T‐lymphocytes and FOXP3+ infiltrates in primary tumours positively correlated with that of metastatic lesions (ρ = 0.512, P = 0.038 and ρ = 0.698, P = 0.007, respectively), while a strong trend existed for CD204+ infiltrates (ρ = 0.404, P = 0.087). We also observed T‐ and B‐lymphocytes, and CD204+ macrophages to be significantly higher in a dog's pulmonary metastasis compared to their primary tumour (P = 0.018, P = 0.018, P = 0.016, respectively), while FOXP3+ cells were only significantly higher in metastases when all primary tumour and metastasis lesions were compared without pairing (P = 0.036). Together, these findings suggest that the metastatic immune microenvironment may be influenced by that of the primary cOSA, and that primary tumour immune biomarkers could potentially be applied to predict immunotherapeutic responses in gross metastatic disease. We, therefore, provide a rationale for the treatment of cOSA pulmonary metastases with immunotherapeutics that enhance the anti‐tumour activity of these immune cells, particularly in dogs with moderate to high immune cell infiltration in their primary tumours. 相似文献
58.
Ribonuclease 6 (RNase6 or RNase K6) is a protein that belongs to a superfamily thought to be the sole verte‐brate‐specific enzyme known for a wide range of physiological functions, including digestion, cytotoxicity, angiogenesis, male reproduction and host defense. In our study, 51 functional genes and 11 pseudogenes were identified from 27 Rodentia species. Intriguingly, in the 3 main lineages of rodents there were multiple RNase6s identified in all species of Ctenohystrica, whereas only a single RNase6 was observed in other Rodentia species examined except for 2 species in the mouse‐related clade. The evolutionary scenario of “birth (gene duplication) and death (gene deactivation)” and gene sorting have been demonstrated in Ctenohystrica. In addition, bursts of positive selection, diversification of isoelectric point and positive net charge have been identified in Ctenohystrica, especially at two key sites that are involved in antimicrobial function. Site Trp30 has undergone positive selection and Ile45 has changed into other residues in Group B and Group C of the Ctenohystrica. Our results demonstrated a complex and intriguing evolutionary pattern of rodent RNase6, and indicated that functional modification may have occurred, which establishes an important theoretical foundation for future functional assays in rodent RNase6. 相似文献
59.
Burton EC Miller DL Styer EL Gray MJ 《Veterinary journal (London, England : 1997)》2008,177(3):442-444
During an on-going amphibian ecology study, a free-ranging American bullfrog (Rana catesbeiana) metamorph was captured in a pitfall trap adjacent to a constructed farm pond at the Plateau Research and Education Center (PREC) on the Cumberland Plateau near Crossville, Tennessee, USA. Grossly, the right eye was approximately 50% the size of the left. Stereo and light microscopic examination revealed two granulomas within the orbit. Electron microscopic examination revealed virus particles scattered throughout one structure but mostly aggregated toward the center. Subsequent PCR and sequencing (GenBank accession Number EF175670) confirmed frog virus 3 (FV3). This represents the first report of a malformation in an anuran associated with FV3. 相似文献
60.
Arunvipas P Vanleeuwen JA Dohoo IR Leger ER Keefe GP Burton AS Lissemore KD 《Preventive veterinary medicine》2007,82(1-2):42-50
Our objective of this study was to investigate the relationship between milk urea-nitrogen concentrations ([MUN]) and first-service breeding success (FSBS) in a large number of commercial dairy herds, using various timings on [MUN]. All commercial dairy herds in Prince Edward Island on monthly milk testing (n=198) formed the sampling frame. Milk components, [MUN], 24-h milk production, and breeding data for all cows from these farms were gathered electronically from a central database. A first service between 1 June 1999 and 31 May 2000 was classified successful (FSBS=1) if it was the cow's last service and she calved 270-290 d later. Mixed logistic-regression modeling was used to determine the association between FSBS (the outcome variable) and the [MUN] closest to first service, controlling for other possible confounders and clustering effects of cows within the study herds. The final dataset included 2787 successful and 3015 unsuccessful first services. A change in [MUN] on the test closest to first service from 10 to 20 mg/dL was associated with a 13.9% reduction in the odds of FSBS (controlled for parity, milk production and days in milk). 相似文献