Protective activity against oocyst shedding in cats vaccinated with crude rhoptry proteins of the Toxoplasma gondii by the intranasal route     

Garcia JL  Navarro IT  Biazzono L  Freire RL  da Silva Guimarães Junior J  Cryssafidis AL  Bugni FM  da Cunha IA  Hamada FN  Dias RC 《Veterinary parasitology》2007,145(3-4):197-206

This study evaluated a vaccine made from crude rhoptry proteins of Toxoplasma gondii with Quil-A, which was administered to cats by the intranasal route. Eleven short-hair domestic cats were divided into four groups: G1 (n=3) received three doses (200 microg/dose) of the rhoptry vaccine with Quil-A (20 microg); G2 (n=3) received PBS with Quil-A (20 microg); G3 (n=3) and G4 (n=2) received only PBS. Treatments were administered at days 0, 21, and 42 by the intranasal route. Challenge was done to G1, G2, and G3 animals with 600 cysts of the VEG strain on day 51 (challenge day); G4 animals were unchallenged. The anti-T. gondii IgG and IgA antibody levels from sera were measured by indirect enzyme-linked immunosorbent assay (ELISA). At challenge, two animals from G1 revealed antibody levels for both IgG and IgA; oocysts were not detected in feces of these two cats. There were no differences in hematological values between groups throughout the experiment (p>0.10). Preventable fractions were 67% in G1 and 0% in G2 and G3. Comparatively, G1 animals shed 89.3% and 90.8% less oocysts than G3 and G4, respectively. Two out of three cats were protected against T. gondii oocyst shedding when the rhoptry vaccine was administered by the intranasal route. This is the first study using crude rhoptry proteins as vaccine by the intranasal route in cats to evaluate protection against oocysts shedding.  相似文献   

Post‐hatching development of the ornamental ‘Red Cherry Shrimp’ Neocaridina davidi (Bouvier, 1904) (Crustacea,Caridea, Atyidae) under laboratorial conditions          下载免费PDF全文

João A F Pantaleão  Rafael A Gregati  Rogério C da Costa  Laura S López‐Greco  Maria L Negreiros‐Fransozo 《Aquaculture Research》2017,48(2):553-569

The ‘Red Cherry shrimp’, Neocaridina davidi is a small freshwater caridean shrimp living, originally, in various kinds of inland water bodies around Asian countries. This shrimp has reached several countries for ornamental use; however, basic information on the biology of the species is still scarce in the literature. Its early post embryonic development morphology has not yet been described. This paper focused on the production and the development of early post‐hatching stages of N. davidi, its male secondary sexual features, observation of the gonads and the presence of females with embryos. The larval development of N. davidi was almost suppressed as noted by the presence of relatively large‐sized eggs, first stage hatching as a decapodid; and the tail fans were present only from the 2nd post‐hatching stage. A biological important consequence of the presence of this Red Cherry shrimp species in the Neotropics is its potential release into nature, which could cause its rapid dispersion affecting populations of other indigenous caridean freshwater shrimps.  相似文献   

Environmental impact of razor clam harvesting using salt in Ria Formosa lagoon (Southern Portugal) and subsequent recovery of associated benthic communities     

Rita Constantino  Miguel B. Gaspar  Fábio Pereira  Susana Carvalho  João Cúrdia  Domitília Matias  Carlos C. Monteiro 《水产资源保护:海洋与淡水生态系统》2009,19(5):542-553

• 1. Razor clams are found in different habitats ranging from sheltered systems (e.g. lagoons and estuaries) to open coasts. They are distributed worldwide and comprise a small number of species of high economic value. Depending on the specific habitat and species exploited, different mechanical and hand‐harvesting techniques are employed. While the environmental effects resulting from mechanized methods have been described by several authors, the impacts caused by traditional hand‐harvesting methods remain unknown. Therefore, a study was undertaken in Ria Formosa lagoon (South of Portugal) addressing the environmental effects resulting from harvesting Solen marginatus with salt.
• 2. No significant impact on the sediment was found; the main effect was an increase in salinity after covering the area with salt, which decreased rapidly with the flood tide and after a few hours had returned to pre‐harvesting levels.
• 3. No effects on benthic communities were observed, with similar fluctuation patterns recorded in control and experimental areas, the observed differences being attributed to the natural variability of benthic populations.
• 4. Based on the results obtained in the present study, a razor clam fishery using salt in intertidal areas can be considered environmentally ‘friendly’.

Copyright © 2008 John Wiley & Sons, Ltd.  相似文献   

Edwardsiella tarda infection in Korean catfish,Silurus asotus,in a Korean fish farm     

Jin‐Ha Yu  Jung Jo Han  Kwon Sam Park  Kwan Ha Park  Sung Woo Park 《Aquaculture Research》2009,41(1):19-26

Mass mortality of Korean catfish, Silurus asotus, occurred in a culture farm situated in Jeollabukdo Province, Korea. The cumulative mortality rates reached up to 5% of the total fish in the farm per day. In clinical signs, the affected fish showed abdominal distension, vent protrusion, enteritis, liver congestion and abscess‐like lesions in enlarged spleen and kidney. Histopathologically, in the liver, hepatocytes lost fat and underwent atrophy or necrosis. The spleen showed necrotized splenocytes and a haemorrhagic pulp. In the kidney, glomerular destruction, degeneration of renal tubular epithelial cell and haemorrhage were observed. However, necrotic muscular lesions were not observed. A pure bacterial isolate was obtained from the liver, spleen and kidney lesions of affected fish. Experimental infection of normal catfish with the isolate resulted in the development of clinical signs similar to those seen on the farm. The isolates were identified as Edwardsiella tarda through biochemical tests (99.4%) and analysis of bacterial genes (16S rDNA) sequences (98%). The bacteria possessed two virulent genes: sodB and katB genes. These results suggest that E. tarda can act as a pathogen of farmed catfish. This is the first report showing that E. tarda caused mortality in cultured Korean catfish.  相似文献   

Authentication of Rice by Three-Sided Image Analysis of Kernels Using Two Mirrors     

Sang Sook Kim  Jin Sook Jo  Young Jin Kim  Nae Kyung Sung 《Cereal Chemistry》1997,74(3):212-215

The authentication of rice by imaging three sides (top, side, and front) of a rice kernel using two mirrors was attempted. Nine image characteristics (area, aspect ratio, minimum diameter, maximum diameter, perimeter, and red, green, and blue values) for each side of a rice kernel were measured. Japonica type rices were used for the study; a total of 89 rice samples were used: 40 samples harvested in Korea, 33 in America, 5 in Australia, and 11 in China. Image data collected from 105 kernels per rice sample were used as a calibration data set to develop a model for quadratic discriminant analysis. In overall pairwise trials, on average, 85.8–94.5% correct identification of the cultivated country was obtained, depending on the number of image characteristics used for the discriminant model. For the effective discrimination of the cultivated country, 13 image characteristics were selected. As a test sample set, 20 kernels per sample were used. A model for discriminant analysis with 13 image characteristics yielded an average of 95.0 and 97.5% accuracy for calibration and test sample set, respectively.  相似文献   

Using fish models to investigate the links between microbiome and social behaviour: The next step for translational microbiome research?     

Marta C. Soares  Jo Cable  Monica G. Lima‐Maximino  Caio Maximino  Raquel Xavier 《Fish and Fisheries》2019,20(4):640-652

Recent research has revealed surprisingly important connections between animals’ microbiome and social behaviour. Social interactions can affect the composition and function of the microbiome; conversely, the microbiome affects social communication by influencing the hosts’ central nervous system and peripheral chemical communication. These discoveries set the stage for novel research focusing on the evolution and physiology of animal social behaviour in relation to microbial transmission strategies. Here, we discuss the emerging roles of teleost fish models and their potential for advancing research fields, linked to sociality and microbial regulation. We argue that fish models, such as the zebrafish (Danio rerio, Cyprinidae), sticklebacks (?Gasterosteidae), guppies (Poeciliidae) and cleaner–client dyads (e.g., obligate cleaner fish from the Labridae and Gobiidae families and their visiting clientele), will provide valuable insights into the roles of microbiome in shaping social behaviour and vice versa, while also being of direct relevance to the food and ornamental fish trades. The diversity of fish behaviour warrants more interdisciplinary research, including microbiome studies, which should have a strong ecological (field‐derived) approach, together with laboratory‐based cognitive and neurobiological experimentation. The implications of such integrated approaches may be of translational relevance, opening new avenues for future investigation using fish models.  相似文献   

Microalgae Ankistrodesmus gracilis as feed ingredient for ornamental fish Xiphophorus maculatus     

Sipaúba-Tavares  Lúcia H.  Fernandes  João B. K.  Melo-Santos  Gustavo L.  Scardoeli-Truzzi  Bruno 《International Aquatic Research》2019,11(2):125-134

International Aquatic Research - A trial evaluated the effectiveness of macrophytes, Azolla caroliniana (AAC) and Lemna minor (ALM) as a culture media for microalgae Ankistrodesmus gracilis, and...  相似文献   

Molecular cloning,sequence characterization,and expression analysis of C-type lectin (CTL) and ER-Golgi intermediate compartment 53-kDa protein (ERGIC-53) homologs from the freshwater prawn,Macrobrachium rosenbergii     

Baliarsingh  Snigdha  Sahoo  Sonalina  Jo  Yong Hun  Han  Yeon Soo  Sarkar  Arup  Lee  Yong Seok  Mohanty  Jyotirmaya  Patnaik  Bharat Bhusan 《Aquaculture International》2022,30(2):1011-1035

Aquaculture International - Lectin protein families are diverse and multi-functional in crustaceans. The carbohydrate-binding domains (CRDs) of lectins recognize the molecular patterns associated...  相似文献   

Toward a Resource‐Based View of City Quality: A New Framework          下载免费PDF全文

João M. S. Carvalho  Ricardo V. Costa  Sandra Marnoto  Célio A. A. Sousa  José Carvalho Vieira 《Growth and change》2018,49(2):266-285

Based on an extensive literature review on intelligent cities, smart cities, and happy cities, and on their conceptual connections with citizens' well‐being, quality of life, and happiness, we developed a resource‐based view on City Quality: the PESNAT (political, economic, social, natural, artificial, and technological) framework. The concept of City Quality rests on the idea of cities interconnected sub‐habitats—PESNAT—which are powerful analytical categories needed for understanding cities as complex and intricate loci. This framework eventually aims at assessing the cities' power to attract businesses and people, to contribute to a sustainable development of the city and an increased quality of life. Furthermore, two hypotheses are outlined regarding the level of importance of each sub‐habitat in relation to happiness, and the level of controversy of each one for citizens, city planners, and decision makers.  相似文献   

A new insight into the influence of habitat on the biochemical properties of three commercial sea cucumber species     

González-Wangüemert  Mercedes  Roggatz  Christina C.  Rodrigues  Maria João  Barreira  Luísa  da Silva  Manuela Moreira  Custódio  Luísa 《International Aquatic Research》2018,10(4):361-373

International Aquatic Research - This work makes a comparative evaluation of the biochemical profile of three sea commercial cucumber species (Holothuria mammata, H. polii and H. tubulosa) caught...  相似文献