Effects of exogenous amylase on the in vitro digestion kinetics of whole-crop maize silages made from flint or dent grain type at different phenological stages grown in tropical condition     

Abias Santos Silva  Luiz Gustavo Ribeiro Pereira  Márcio dos Santos Pedreira  Fernanda Samarini Machado  Mariana Magalhães Campos  Cristina Simões Cortinhas  Tiago Sabella Acedo  Rafael Dantas dos Santos  João Paulo Pacheco Rodrigues  Rogério Martins Maurício  Thierry Ribeiro Tomich 《Journal of animal physiology and animal nutrition》2020,104(1):76-87

The effect of exogenous amylase on the in vitro rumen digestion kinetics of whole-crop maize silage made from dent (RB9004) or flint grain type (RB9308) was evaluated at different phenological stages: soft dough (SOD), early dent (EAD), ½ milkline (½M) and ¾ milkline (¾M). Forage was harvested from 70 to 110 days after sowing. Two rumen-cannulated cows receiving or not exogenous amylase (0.7 g/kg dry matter—DM, provided to achieve 396 kilo Novo units of amylase activity/kg of TMR DM) were used as donor of ruminal fluid. The in vitro gas production kinetics was evaluated according to a dual-pool logistic model. The chemical composition and gas production kinetics were affected by the hybrid and phenological stages. The flint hybrid had lower range for chemical analysis among physiological stages. Harvesting at ½M and ¾M improved DM content, bromatological composition and silage quality parameters compared to dent or flint types. Amylase (i) increased methane (CH₄) production and in vitro dry matter digestibility (IVDMD) in ½M stage, (ii) improved digestion kinetics by reducing lag time and increasing total gas production and fermentation rates of non-fibrous carbohydrates (NFC) and fibrous carbohydrates (FC), and (iii) increased extent and fermentation rate of NFC and increased fermentation rate of FC fraction in whole-crop maize silages produced from dent or flint types in all phenological stages. Harvesting between ½M and ¾M is the best phenological stage to improve chemical composition and silage quality parameters. Exogenous amylase showed improvements on fibre digestion of silages at ½M and ¾M phenological stages in both grain types of corn.  相似文献   

Combinations of cactus pear with different roughage sources on the production,chemical composition,and milk fatty acid profile of F1 Holstein/Zebu cows     

Soares  Camila  Rocha Júnior  Vicente Ribeiro  Monção  Flávio Pinto  Borges  Lucas Daniel Alcântara  Caldeira  Luciana Albuquerque  Costa  Natanael Mendes  Ruas  José Reinaldo Mendes  Rigueira  João Paulo Sampaio  da Cunha Siqueira Carvalho  Cinara  de Sales  Eleuza Clarete Junqueira  de Assis Pires  Daniel Ananias  Chamone  Julieta Maria Alencar 《Tropical animal health and production》2020,52(5):2567-2576

Tropical Animal Health and Production - The qualities of food, mainly of animal origin, have always been of concern to consumers. It is known that the composition of animals’ diets can...  相似文献   

Optimising the DPPH Assay for Cell-Free Marine Microorganism Supernatants     

Yehui Gang  Tae-Yang Eom  Svini Dileepa Marasinghe  Youngdeuk Lee  Eunyoung Jo  Chulhong Oh 《Marine drugs》2021,19(5)

Antioxidants prevent ageing and are usually quantified and screened using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. However, this assay cannot be used for salt-containing samples, such as the cell-free supernatants of marine microorganisms that are aggregated under these conditions. Herein, the DPPH solvent (methanol or ethanol) and its water content were optimized to enable the analysis of salt-containing samples, aggregation was observed for alcohol contents of >70%. The water content of methanol influenced the activities of standard antioxidants but did not significantly affect that of the samples. Based on solution stability considerations, 70% aqueous methanol was chosen as the optimal DPPH solvent. The developed method was successfully applied to the cell-free supernatants of marine bacteria (Pseudoalteromonas rubra and Pseudoalteromonas xiamenensis), revealing their high antioxidant activities. Furthermore, it was concluded that this method would be useful for the screening of marine microorganism–derived antioxidants, which also has numerous potential applications, such as salt-fermented foods.  相似文献   

Modified atmosphere container equipped with gas diffusion tube automatically controlled in response to real-time gas concentration     

Yun Hee Jo  Nam Yong Kim  Duck Soon An  Hyuek Jae Lee  Dong Sun Lee 《Biosystems Engineering》2013,115(3):250-259

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Risk assessment of Aphanomyces euteiches root rot disease: quantification of low inoculum densities in field soils using droplet digital PCR     

Gibert  Simon  Edel-Hermann  Véronique  Moussa Mcolo  Rayanti  Gautheron  Elodie  Michel  Joël  Bernaud  Eric  Gautheron  Nadine  Sol  Jean-Marie  Capelle  Gery  Galland  Rachel  Bardon-Debats  Arnaud  Lambert  Claudine  Steinberg  Christian 《European journal of plant pathology / European Foundation for Plant Pathology》2021,160(3):503-518

European Journal of Plant Pathology - Fusarium verticillioides is a soil-borne plant pathogen of maize plants (Zea mays L.) responsible for major yield losses by causing root, stalk, and ear rot...  相似文献   

The NURBS human body modeling using local knot removal     

Joon Woo Jo  Sung Soo Han 《Fibers and Polymers》2005,6(4):348-354

These days consumers’ various demands are accelerating research on apparel manufacturing system including automatic measurement, pattern generation, and clothing simulation. Accordingly, methods of reconstructing human body from point-clouds measured using a three dimensional scanning device are required for apparel CAD system to support these functions. In particular, we present in this study a human body reconstruction method focused on two issues, which are the decision of the number of control point for each sectional curve with error bound and the local knot removal for reducing the unusual concentration of control points. The approximation of sectional curves with error bounds as an approximation criterion leads all sectional curves to their own particular shapes apart from the number of control points. In addition, the application of the local knot removal to construction of human body sectional curves reduces the unusual concentration of control points effectively. The results may be used to produce an apparel CAD system as an automatic pattern generation system and a clothing simulation system through the low level control of NUBS or NURBS.  相似文献   

The fabrication and characterization of the photovoltaic cells composed of polydiacetylene and fullerene     

Jeong Ho Song  Tae Jo Kang  Young Don Cho  Sun Hyoung Lee  Jeong Soo Kim 《Fibers and Polymers》2006,7(3):217-222

Propargyl alcohol was coupled to 2,4-hexadiyne-1,6-diol (HDD) and crystallized in the process of ultraviolet irradiation-induced topochemical polymerization. The HDD polymer crystals were used as one component in the fabrication of organic photovoltaic cells, in combination with fullerene as the electron acceptor. The various structures of the produced photovoltaic cells included bilayer, trilayer, and bulk heterojunction structures. Their photovoltaic properties were analyzed in relation to crystal structure, electrochemical properties, and band structure of the HDD polydiacetylene polymers.  相似文献   

Genetics components of rice root architecture and carbon isotopic fractionation parameters: a tracer for breeding in a water-saving irrigation management     

Brito  Giovani G.  Concenço  Germani  Costa  Vladimir E.  Fagundes  Paulo Ricardo R.  da Silva-Filho  João Luis  Parfitt  José Maria B.  Magalhães  Ariano  Silva  Giovana T.  Jardim  Thaís M.  Luccas  Nathália Furtado  Scivittaro  Walkyria B. 《Journal of Crop Science and Biotechnology》2022,25(1):19-32

Journal of Crop Science and Biotechnology - Phenotyping is the major bottleneck in the effort to develop varieties of rice (Oryza sativa L.) suitable for growing under a water-saving irrigation...  相似文献   

Effect of Equilibrium Solution Ionic Strength on the Adsorption of Zn,Cu, Cd,Pb, As,and P on Aluminum Mining By-Product     

de S. Costa  Enio Tarso  Guilherme  Luiz Roberto G.  Lopes  Guilherme  Marques  João José  Curi  Nilton 《Water, air, and soil pollution》2014,225(3):1-11

Adsorption onto powdered activated carbon (PAC) is a promising option to remove organic micro-pollutants (OMP) from drinking water sources or wastewater. Since this treatment option requires continuous PAC dosing, sufficient contact time and subsequent separation of the PAC, the integration into existing process chains is challenging. In the present investigation, the pre-loading of a deep bed filter with PAC used as fixed bed adsorber was investigated. The retention and distribution of an exemplary PAC in a pumice rapid filter were determined. Gravimetry combined with combustion of the PAC at 550 °C was applied to differentiate between PAC and filter material residues and revealed comparably high PAC immobilization in the upper third of the pumice filter. Comparative adsorption experiments in batch with suspended PAC and continuous filtration tests with immobilized PAC showed advantageous results for immobilized PAC with regard to the removal of OMP and the sum parameters dissolved organic carbon and UV light absorption at 254 nm wavelength. The results indicate that a conventional rapid filter together with PAC can be effectively utilized as fixed bed adsorption filter.  相似文献   

Iron isotope fractionation in soil and graminaceous crops after 100 years of liming in the long-term agricultural experimental site at Berlin-Dahlem,Germany     

Bei Wu  Yi Wang  Anne E. Berns  Kathlin Schweitzer  Sara L. Bauke  Roland Bol  Wulf Amelung 《European Journal of Soil Science》2021,72(1):289-299

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