Isolation of equine herpesvirus-2 from the lung of an aborted fetus.     

Cecilia Mónica Galosi  Viviana Cid de la Paz  Leandro Carlos Fernández  Javier Pablo Martinez  María Isabel Craig  María Barrandeguy  María Elisa Etcheverrrigaray 《Journal of veterinary diagnostic investigation》2005,17(5):500-502

This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause-effect relationship was established between the EHV-2 isolate and the abortion.  相似文献   

Evaluation of an air-filtration system for preventing aerosol transmission of Porcine reproductive and respiratory syndrome virus          下载免费PDF全文

Scott Dee  Laura Batista  John Deen    Carlos Pijoan 《Canadian journal of veterinary research》2005,69(4):293-298

The purpose of this study was to evaluate the ability of a commercial air-filtration system to reduce aerosol transmission of Porcine reproductive and respiratory syndrome virus (PRRSV). The system consisted of a pre-filter and 2 filters with EU8 and EU13 ratings. In each of 4 trials, 5 PRRSV-infected donor pigs and 1 naive recipient pig (each 25 kg) were housed in opposing chambers connected by a 1.3-m-long duct. The system filtered air entering 1 recipient-pig chamber (filtered facility) from the donor-pig chamber but not a 2nd recipient-pig chamber (nonfiltered facility). The donor pigs had been experimentally infected with PRRSV MN-184, an isolate previously documented to be shed at a high frequency in contagious aerosols. On days 3 to 7 after infection of the donors, the 2 groups were housed in their respective chambers for 6 h and then in separate facilities, where samples were collected for testing by polymerase chain reaction and enzyme-linked immunosorbent assay over 14 d. Aerosol transmission was observed in 6 of the 20 replicates in the nonfiltered facility, whereas all pigs remained PRRSV-negative in the filtered facility; the difference was significant at P < 0.01. Thus, under the conditions of this study, the air-filtration system evaluated appeared to be highly effective at reducing aerosol transmission of PRRSV.  相似文献   

Renal handling of calcium and phosphorus in experimental renal hyperparathyroidism in dogs   总被引：3，自引：0，他引：3  

García-Rodríguez MB  Pérez-García CC  Ríos-Granja MA  Cano-Rábano MJ  Peña-Penabad M  Gallego-Morales D  García-Partida P  Diez-Prieto I 《Veterinary research》2003,34(4):379-387

Twenty-four hour urinary excretion, fractional excretion and the filtered load of calcium and phosphorus were monitored as hyperparathyroidism evolved in a model of progressive canine renal failure. Thirteen beagles of both sexes aged four and a half months were used. Nine of them were subjected to a renal damaging schedule (neomycine, 60 mg/kg/48 h, IM, 32 weeks) in order to induce chronic renal failure leading to secondary hyperparathyroidism (2HPT group). The remaining four were kept as the control group. The experiment was conducted over 32 weeks. Blood and 24 h urine were collected every four weeks. Calcium, phosphorus and creatinine were analyzed. Plasma parathormone and calcitonin were determined at weeks 0, 12, 24 and 32. The level of renal function in the 2HPT animals was reduced to 25% of that of the controls (endogenous creatinine clearance was 0.45 +/- 0.22 mL/min/kg as opposed to 1.81 +/- 0.54 mL/min/kg). Hyperparathyroidism was confirmed by a progressive increase in the levels of the parathyroid hormone. Calcitonin levels were not modified. A tendency to hypocalcaemia was observed, reaching statistically significant levels from the twenty-eighth week of the study, when hyperphosphataemia also became significant. Daily urinary excretion of calcium and phosphorus remained at values considered normal throughout the experiment with no alteration imputable to the impaired renal function. This is explained by the decrease in the filtered load of these elements (in both cases statistically significant from the 24th week on) being associated with an increase in their fractional excretion. Thus, calcium and phosphorus urinary excretion values could be maintained in a normal range up to the end of the experiment, showing that renal calcium handling in dogs with experimentally induced renal failure seems to differ from that observed in human patients.  相似文献   

Fine structure of Henneguya pilosa sp. n. (Myxozoa: Myxosporea), parasite of Serrasalmus altuvei (Characidae), in Brazil     

Azevedo C  Matos E 《Folia parasitologica》2003,50(1):37-42

Henneguya pilosa sp. n., a new species of myxosporean from the gill filaments of the white piranha, Serrasalmus altuvei Ramirez, 1965 (Characidae), a freshwater teleost fish collected in the Zoological Garden of the city of Teresina (Piauí), Brazil, is described from light and transmission electron microscope observations. This myxosporean produced small plasmodia (up to 0.2 mm in diameter), each one containing all life-cycle stages of the parasite, including numerous spores. The spores, laterally compressed, averaged 54.2 (52.3-56.0) microm in total length and consisted of two unequal valves adhering together along the suture line and two caudal processes. The spore body measured 21.1 (20.0-23.1) microm in length, 5.9 (5.5-6.3) microm in width, and 2.2 (1.9-2.6) microm in thickness. The two equal ellipsoidal polar capsules of 7.4 (7.1-7.6) microm long and 1.2 (1.0-1.3) microm wide possessed a polar filament with 11-12 (rarely 13) turns. All surfaces of the spores were covered with a tightly adherent complex network of numerous densely ramified granulo-fibrillar masses, the longest measuring 1.5 microm long, observed around the caudal processes. The prevalence of infection was 30%. The taxonomic affinities of this parasite with other of the same genus in freshwater South American fish species are discussed.  相似文献   

Component population study of Acanthocephalus tumescens (Acanthocephala) in fishes from Lake Moreno,Argentina     

Rauque CA  Viozzi GP  Semenas LG 《Folia parasitologica》2003,50(1):72-78

Seasonal samples of all fish species from Lake Moreno were taken in order to determine the presence of paratenia, to evaluate the status of the hosts and to characterise the transmission of Acanthocephalus tumescens (von Linstow, 1896) at the component population level. Prevalence, mean abundance, mean intensity, numbers of gravid females, relative abundance of the different fish species, relative output of eggs and relative flow rates for each host species were computed. Acanthocephalus tumescens showed low host specificity, successfully parasitizing six out of eight fish species present in the lake. No paratenic infection was registered. If prevalence, mean abundance, and number of gravid females are considered, host species can be placed in a continuum from the most to least suitable as follows: Galaxias platei Steindachner, Diplomystes viedmensis (Mac Donagh), Oncorhynchus mykiss (Walbaum), Salvelinus fontinalis (Mitchill), Percichthys trucha (Cuvier et Valenciennes) and Galaxias maculatus (Jenyns). However, when parasite flow rates and egg output were calculated, including relative abundance of each fish species, the continuum was rearranged as follows: P. trucha, O. mykiss, G. platei / G. maculatus, S. fontinalis and D. viedmensis. The first four species would be the main contributors to the population of A. tumescens in this lake, P. trucha being the major one. Different regulatory and non-regulatory mechanisms are suggested.  相似文献   

Prevalence of antibodies against Ornithobacterium rhinotracheale in broilers and breeders in Southern Brazil     

Canal CW  Leão JA  Ferreira DJ  Macagnan M  Pippi Salle CT  Back A 《Avian diseases》2003,47(3):731-737

In this investigation, we determined the prevalence of the Ornithobacerium rhinotracheale (ORT) infection in broilers and broiler breeders in southern Brazil. We also correlated the presence of antibodies in broilers with performance. Sera from 1550 broilers from 50 flocks were collected during the slaughter time in nine companies with federal veterinary inspection of the state of Rio Grande do Sul. Sera from 480 meat-type breeders of 40 flocks from 14 companies in southern Brazil were also analyzed by enzyme-linked immunosorbent assay, and the prevalence of antibodies was determined. The prevalence of ORT antibodies in broiler flocks was 63.83%, but in each individual flock only 6.52% of the birds were positive. The prevalence in broiler breeder flocks was 100.00%, and in each individual flock 94.62% of the birds were positive. There was a positive correlation between the presence of antibodies to ORT and decreased body weight in broilers. There was no significant correlation between presence of antibodies to ORT and age, lineage, efficiency index, feed conversion, and mortality. There was a positive correlation between the presence of respiratory signs and antibodies to ORT, although the reverse correlation was not significant. These results confirm that ORT is present and widespread in broilers and broiler breeders in southern Brazil.  相似文献   

Osteomyelitis associated with an orthopedic implant          下载免费PDF全文

Rahal SC  Mamprim MJ  Caporali EH  Teixeira CR 《The Canadian veterinary journal. La revue veterinaire canadienne》2003,44(7):597-599

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Larvicidal effects of several chemicals on Strongyloides infective larvae     

Rebollo CD  Taira N  Ura S  Williams JC 《Veterinary parasitology》2003,118(1-2):165-168

The larvicidal effects of 11 anthelmintics, 7 pesticides and 4 disinfectants were evaluated with infective larvae of Strongyloides papillosus (SPL) and Strongyloides venezuelensis (SVZ). The lethal concentrations against SPL and SVZ were found to be similar. Three chemicals (dichlorvos, levamisole and trichlorfon) showed highest larvicidal effects. The 50% lethal concentration (LC(50)) values for the three compounds against SPL larvae were 0.08, 0.24, and 0.59 ppm, respectively.  相似文献   

Disseminated pythiosis in three horses     

Reis JL  de Carvalho EC  Nogueira RH  Lemos LS  Mendoza L 《Veterinary microbiology》2003,96(3):289-295

Three cases of equine subcutaneous pythiosis with dissemination to the internal organs were investigated. The subcutaneous lesions were observed on the mammary gland, nostrils and limbs of the infected horses. Histopathological analysis of the infected tissues revealed a strong eosinophilic reaction, with macrophages, mast cells and giant cells. Sparsely septated hyphal filaments of 4-6 microm diameter were identified in the center of the eosinophilic areas. Specific fluorescent antibody against Pythium insidiosum confirmed the hyphae in the infected tissues in three examined horses. In one of the three cases, the DNA sequences amplified from the infected subcutaneous tissues and internal organs, revealed that P. insidiosum's 18S SSU rDNA amplicons shared 100% identity with those sequences deposit in GenBank. This is the first report confirming by immunochemical and genetic techniques that P. insidiosum can disseminated from superficial to deep structures.  相似文献   

Occurrence of the temperature-sensitive hemagglutinin among avian Escherichia coli     

Delicato ER  de Brito BG  Konopatzki AP  Gaziri LC  Vidotto MC 《Avian diseases》2002,46(3):713-716

In this study, we determined the occurrence of the tsh gene among 305 Escherichia coli isolates from chickens by means of the polymerase chain reaction and agglutination of chicken erythrocytes; 200 of those isolates were obtained from chickens with colisepticemia, 52 isolates were from lesions of cellulitis, and 53 were from feces of normal chickens. The tsh gene was found in 79 (39.5%) isolates from colisepticemia, in 10 (19%) cellulitis-derived E. coli isolates, and in two (3.8%) fecal isolates. Among the tsh+ strains, 68 (86%) isolates from colisepticemia and nine (90%) from cellulitis agglutinated chicken erythrocytes in the presence of mannose, after growing the strains on colonization factor antigen agar plates at 26 C, which confirms a correlation between mannose-resistant hemagglutination and expression of hemagglutinin Tsh. These results show, for the first time, the presence of the gene tsh in cellulitis-derived E. coli isolates; the high frequency of this gene among avian pathogenic E. coli isolates in Brazil indicates that its putative role as a virulence factor should be studied more thoroughly.  相似文献