首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   853篇
  免费   39篇
林业   78篇
农学   26篇
基础科学   6篇
  277篇
综合类   31篇
农作物   77篇
水产渔业   108篇
畜牧兽医   197篇
园艺   23篇
植物保护   69篇
  2024年   1篇
  2023年   5篇
  2022年   7篇
  2021年   17篇
  2020年   20篇
  2019年   36篇
  2018年   30篇
  2017年   33篇
  2016年   26篇
  2015年   23篇
  2014年   44篇
  2013年   49篇
  2012年   66篇
  2011年   68篇
  2010年   36篇
  2009年   39篇
  2008年   73篇
  2007年   60篇
  2006年   43篇
  2005年   42篇
  2004年   36篇
  2003年   29篇
  2002年   41篇
  2001年   11篇
  2000年   10篇
  1999年   3篇
  1998年   5篇
  1997年   5篇
  1996年   2篇
  1995年   7篇
  1994年   4篇
  1993年   2篇
  1992年   3篇
  1989年   2篇
  1987年   1篇
  1986年   3篇
  1984年   2篇
  1982年   1篇
  1981年   1篇
  1980年   2篇
  1977年   1篇
  1976年   1篇
  1971年   1篇
  1966年   1篇
排序方式: 共有892条查询结果,搜索用时 31 毫秒
11.
The Chato Murciano pig is a breed catalogued as a Special Protection breed in danger of extinction. At present it is commonly reared in an intensive system. We have studied the mineral composition and other characteristics of its carcass and meat. Samples from the Musculus longissimus lumborum were taken from a total number of 43 animals, divided into two groups, Chato Murciano and Chato Murciano crossed with Iberian, reared in a conventional indoor system. The resulting meat of the Chato Murciano pig and its cross with Iberian was especially rich in iron and copper, rich in phosphorus, and marginally poor in calcium and sodium. The evolution of the pH of the carcass was that of pigs which do not produce PSE (pale, soft and exudative) meat. Some relevant positive correlation coefficients between minerals (P ≤ 0.05) have been found.  相似文献   
12.
Santiago, Chile's semi-arid climate and urbanized environment poses a severe limitation for the establishment and maintenance of urban forests. Municipalities, or comunas, are the main stakeholders in the management of Santiago's public urban forests. A tenable hypothesis would be that as the socioeconomic level of a comuna increases, the better the condition of a comuna's urban forest. Unfortunately, there is little comprehensive information on management, public expenditure, and structure of Santiago's public and private urban forests. To examine this hypothesis, Santiago was divided into socioeconomic strata, then using air photo interpretation and stratified field sampling, urban forest structures were quantified by socioeconomic strata. In addition, interview surveys were used to determine municipal urban forest management and expenditures for different public urban forests based on socioeconomic strata. Urban forests in the high socioeconomic strata had fewer public trees, greater tree cover, tree and leaf area density, and leaf area index than lower socioeconomic strata. The percentage of total municipal budget allocated to public urban forest management was consistent among strata, but the total public urban forest budgets were greater in the high socioeconomic strata. Public urban forest structure is related to the socioeconomic strata of Santiago's different comunas.  相似文献   
13.
The European corn borer (Ostrinia nubilalis) is one of the most important pests of maize (Zea mays). Injuries to the plants caused by the larvae of the European corn borer may represent entrance gates for fungal-spores. The cultivation of Bacillus thuringiensis maize (Bt-maize) is one possibility to reduce infestation by the European corn borer. The aim of the present project was to determine and to compare the number of species and the frequency of Fusarium spp. infestation in Bt-maize (cry1Ab) and conventional maize. In 2003, we analysed the Fusarium spp. infestation of samples of chaffed Bt-maize and its isogenic variety on two experimental fields in the Oderbruch region (Germany), an European corn borer infested area. The conventional variety on the first of the experimental fields (previous crop wheat and forking cultivation) showed a small infestation (16%) of Ostrinia nubilalis while in the conventional variety on the second field (previous crop maize and not forking cultivation) the infestation of the European corn borer was almost three times higher (47%). In the conventional variety on both of the experimental fields we found a high Fusarium spp. infestation (70%). Especially species of the section Liseola dominates, among them: F. subglutinans, F. proliferatum und F. verticillioides. The Fusarium infestation in the samples of Bt-maize from the field with previous crop wheat and forking cultivation was just as high as in the conventional variety (70%). The infestation of Fusarium spp. in the samples of Bt-maize from the field with previous crop maize and forking cultivation was more than 20?% lower.  相似文献   
14.
Real-time PCR was used to detect and quantify Verticillium dahliae and to assess the susceptibility of four Capsicum annuum cultivars (Luesia, Padrón, SCM331 and PI201234) and the Capsicum chinense cv. C118 to this pathogen. The symptoms which developed after infection included stunting and yellowing, and were more acute in the cv. SCM331, which also suffered defoliation in later stages of the disease and in C118, which suffered severe stunting. Quantification of the pathogen DNA in roots 23 and 34 days post-inoculation (dpi) revealed that there were significantly higher amounts of Verticillium dahliae DNA in C118 than in the other cultivars, followed by SCM331, Padrón and PI201234. The lowest amounts of fungal DNA in roots were found in Luesia. In hypocotyls, the highest amounts of fungal DNA were found in SCM331, while Luesia, Padrón and PI201234 had much lower amounts, and C118 had intermediate levels. When a compatible versus an incompatible system was studied, using the near-isogenic tomato lines LA3030 (susceptible) and LA3038 (resistant to V. dahliae), we were able to detect fungal DNA in both lines. As expected, the fungus/plant DNA ratio was lower in LA3038 than in LA3030 and it decreased with time in LA3038. The amount of Verticillium dahliae DNA in the roots of LA3030 remained constant between days 23 and 34 post-inoculation, but increased 10-fold in collars. Finally, when real-time PCR was applied as a diagnostic method to samples from pepper plants, soil and water collected from farms in northwest Spain, we were able to detect V. dahliae DNA in these samples even when symptoms of the disease were not evident.  相似文献   
15.
We have analyzed the influence of the stage of ripeness on L-phenylalanine ammonia-lyase (PAL) gene expression, accumulation of anthocyanins and total phenolics, and on antioxidant activity in the skin of table grapes treated with 20% CO2 + 20% O2 + 60 % N2 for 3 or 6 d at low temperature (0 °C). The residual effect of high CO2 treatment after transfer to air was also studied. In early harvested grapes, neither the anthocyanin content nor the accumulation of VcPAL mRNA was affected by any of the CO2 treatments applied. However, in late harvested grapes, the duration of high CO2 treatment determined its effect and a 6 d treatment with CO2 sustained higher levels of total phenolics and anthocyanin accumulation, and VcPAL expression than observed in untreated late harvested grapes. The increased antioxidant capacity was correlated with the total amount of phenolics and anthocyanins. Conversely, in grapes treated for 3 d with CO2 the phenylpropanoid pathway did not appear to be induced and a relationship between antioxidant activity and anthocyanins was not observed. Thus, further studies are needed to identify the most important antioxidants in these treated fruit.  相似文献   
16.
Across the physiognomic types of the Orinoco llanos, periodic inventories and changes in land-use between 1982–1992 are estimated. Results indicate that the area under pastures and forest plantations is increased by 0.005337×106 km2, whilts reducing the area of croplands by 0.000119×106 km2. This is a net increase of 0.005218×106 km2. The gross carbon release is 174.66 Tg C per year to the atmosphere and transferring from cultivated and native vegetation to wood products (1.62 Tg C per year) and slash (1.18 Tg C per year). The processes of land preparation contribute 1.40 Tg C per year to the atmosphere. From the tree savannas, woodlands and forests 0.73 Tg C per year are estimated to have been transferred to the soil following clearance and burning over this period, and 1.05 Tg C per year from herbaceous savannas when were buried and decomposed at 0.84 Tg C per year. The estimate of carbon balance here by inventories and changes in land-use approach indicates that the Orinoco llanos is a sink of −17.53 Tg C per year. The carbon turnover time in the Orinoco system is 68 years, which provides a limited route for carbon sequestration. The calculated potential of the Orinoco llanos for storing carbon is 8300 Tg C. Ecological options to achieve this potential value are addressed. However, nutrient deficiency and seasonal water supply are serious drawbacks to take into account for increasing carbon accretion. These results are particular for the Orinoco llanos, even though described processes could be similar to world-wide savannas, where a gradient of carbon heterogeneity exists.  相似文献   
17.
18.
Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and used to analyze chloroplast diversity of Citrus and closely related genera. Fourteen cpSSR primer pairs from the chloroplast genomes of tobacco (Nicotiana tabacum L.) and Arabidopsis were found useful for analyzing the Citrus chloroplast genome (cpDNA) and recoded with the prefix SPCC (SSR Primers for Citrus Chloroplast). Eleven of the 14 primer pairs revealed some degree of polymorphism among 34 genotypes of Citrus, Fortunella, Poncirus and some of their hybrids, with polymorphism information content (PIC) values ranging from 0.057 to 0.732, and 18 haplotypes were identified. The cpSSR data were analyzed with NTSYS-pc software, and the genetic relationships suggested by the unweighted pair group method based on arithmetic means (UPGMA) dendrogram were congruent with previous taxonomic investigations: the results showed that all samples fell into seven major clusters, i.e., Citrus medica L., Poncirus, Fortunella, C. ichangensis Blanco, C. reticulata Swingle, C. aurantifolia (Christm.) Swingle and C. grandis (L.) Osbeck. The results of previous studies combined with our cpSSR analyses revealed that: (1) Calamondin (C. madurensis Swingle) is the result of hybridization between kumquat (Fortunella) and mandarin (C. reticulata), where kumquat acted as the female parent; (2) Ichang papeda (C. ichangensis) has a unique taxonomic status; and (3) although Bendiguangju mandarin (C. reticulata) and Satsuma mandarin (C. reticulata) are similar in fruit shape and leaf morphology, they have different maternal parents. Bendiguangju mandarin has the same cytoplasm as sweet orange (C. sinensis), whereas Satsuma mandarin has the cytoplasm of C. reticulata. Seventeen PCR products from SPCC1 and 21 from SPCC11 were cloned and sequenced. The results revealed that mononucleotide repeats as well as insertions and deletions of small segments of DNA were associated with SPCC1 polymorphism, whereas polymorphism generated by SPCC11 was essentially due to the variation in length of the mononucleotide repeats.  相似文献   
19.
20.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号