Antimutagenic and anticarcinogenic effect of methanol extracts of Petasites japonicus Maxim leaves     

Hwan-Goo Kang  Sang-Hee Jeong  Joon-Hyoung Cho 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(1):51-58

The methanol extract from the leaves of Petasites japonicus Maxim (PJ) was studied for its (anti-)mutagenic effect with the SOS chromotest and reverse mutation assay. The (anti-)carcinogenic effects were evaluated by the cytotoxicity on human cancer line cells and by the function and the expression of gap junctions in rat liver epithelial cell. PJ extracts significantly decreased spontaneous β-galactosidase activity and β-galactosidase activity induced by a mutagen, ICR, in Salmonella (S.) typhimurium TA 1535/pSK 1002. All doses of the extract (0.08-100 mg/plate) decreased the reversion frequency induced by benzo (α)pyrene (BaP) in S. typhimurium TA 98. It decreased not only the spontaneous reversion frequency but also that induced by BaP in S. typhimurium TA 100. PJ extract showed greater cytotoxic effects on human stomach, colon and uterus cancer cells than on other cancer cell types and normal rat liver epithelial cells. Dye transfers though gap junctions were significantly increased by PJ extracts at concentrations greater than 200 µg/mL and the inhibition of dye transfer by 12-O-tetradecanoylphorobol-13-acetate (TPA) was obstructed in all concentrations of PJ. PJ significantly increased the numbers of gap junction protein connexin 43, and increased the protein expression decreased by TPA in a dose-dependent manner. Based on these findings, PJ is suggested to contain antimutagenic and anticarcionogenic compounds.  相似文献   

Effects of dietary substitution of fishmeal with the combined dry microalgae,Nannochloropsis oceanica (NO) biomass residue and casein on growth and body composition of juvenile abalone (Haliotis discus)          下载免费PDF全文

Sung Hyo Myung  Won‐Gwan Jung  Hee Sung Kim  Ye Eun Kim  Sung Hwoan Cho  Min Seok Jwa  Pil Youn Kim  Mun Kwan Kim  Min‐Woo Park  Byeng‐Hak Kim 《Aquaculture Research》2016,47(1):341-348

The effects of dietary substitution of fishmeal with the combined dry Nannochloropsis oceanica (NO) biomass residue and casein on growth performance and carcass composition of juvenile abalone was determined. One thousand two hundred and sixty juvenile abalone were randomly distributed into the 18 L plastic rectangular containers. Five experimental diets in triplicate were prepared. The 28% fishmeal was included into the control (Con) diet. The 25%, 50%, 75% and 100% fishmeal were substituted with the combination of graded levels of dry NO biomass residue and casein, referred to as the NO25, NO50, NO75 and NO100 diets respectively. Finally, the salted sea tangle (ST), Laminaria japonica was prepared to compare the effect of the experimental diets on performance of abalone. The essential amino acids, such as isoleucine, leucine, phenylalanine, threonine and valine tended to increase with dietary substitution of fishmeal with NO biomass residue in the experimental diets. Arginine tended to decrease with dietary substitution of fishmeal with NO biomass residue. Survival of abalone fed the experimental diets was higher than that of abalone fed the ST diet for 16 weeks. Weight gain and specific growth rate (SGR) of abalone fed the NO100 diet were higher than those of abalone fed the all other diets. Weight gain and SGR of abalone linearly increased with dietary substation of fishmeal with NO. Shell length of abalone tended to increase with dietary substitution of fishmeal with the combined dry NO biomass residue and casein. The chemical composition of the soft body of abalone was different among treatments except for moisture content. In conclusion, fishmeal in the diets for abalone could be completely replaced with the combined dry NO biomass residue and casein when the 28% fishmeal was included.  相似文献   

Dermal melanocytoma-acanthoma in an adult mixed breed dog     

Park SI  Cho KO 《The Japanese journal of veterinary research》2010,58(3-4):165-169

A cutaneous melanocytoma-acanthoma was diagnosed in a 7-year-old intact female mixed breed dog. Grossly, this tumor was a solitary and darkly pigmented nodule located in the face. Histologically, the lesions consisted of melanocytic and epithelial tumor cells. The melanocytic component consisted predominantly of large round-to-polygonal and heavily pigmented melanocytic cells arranged in nests and clusters. These melanocytes were positive for S-100 and vimentin. The epithelial component was composed of multiple small horn cysts with concentric keratin within the cyst lumina and was positive for cytokeratin. Atypism was not observed in both components. Since this tumor has previously been reported in only two dogs, this report adds to the data that will help determing predilections of age, breed, sex and site.  相似文献   

Revitalizing Mountain Ginseng Cultivation in North Jeolla Province,South Korea     

Hag Mo Kang  Sung Jong Cho  Soo Im Choi  Noriko Sato  Hyun Kim 《Small-Scale Forestry》2016,15(4):497-516

A study was made of measures to revitalize mountain ginseng cultivation in the eastern mountainous area of Muju, Jinan and Jangsu municipalities of North Jeolla province, South Korea. The targeted area is typical forested land and is utilized for the cultivation of ginseng. Access to seeds and seedlings for mountain ginseng cultivation is relatively easy in North Jeolla province, and the species is cultivated widely (on 63 % of the area of the province and by 74 % of farmers). This study investigated the current state of mountain ginseng cultivation and business management, including production costs and subsidy, sales and distribution system, and other business management concerns. A survey was conducted on 86 mountain ginseng-cultivating farming households living in the targeted area in 2013. It is concluded that to achieve greater production an aggressive government policy promoting the renting out of suitable sites for mountain ginseng cultivation in national and public production forestland to individual farms and farming cooperatives is necessary. Further research on increasing the survival rate of cultivated mountain ginseng (CMG) until harvesting and preventing damage by wild animals is also deemed necessary. More effective use of related municipal budget subsidy needs to be supported selectively to the enterprising farmers who aggressively pursue mountain ginseng cultivation and business management. Opening CMG retail stores to realize sustainable income growth and developing various processed products will increase farm income. The government needs to introduce policies to sustain consumer trust in CMG through initiatives such as establishing a CMG quality management system for growers, distributing government-approved seeds, and implementing a government-based CMG purchase system.  相似文献   

Adsorption by coniferous leaves of chromium ions from effluent     

Nam-Seok Cho  Masakazu Aoyama  Kazuto Seki  Naotaka Hayashi  Shuichi Doi 《Journal of Wood Science》1999,45(3):266-270

The chromium adsorption ability of leaves from 34 conifer species were examined. Among them, deciduous conifer species, such asLarix, Ginkgo, Metasequia, andTaxodium, showed high ability to adsorb chromium ions. Factors affecting chromium adsorption were studied using larch (Larix leptolepis) leaves to determine the optimum adsorption conditions. The factors included solution pH, contact time, temperature, and the initial concentration of chromium ions. Maximum adsorption for Cr³⁺ was observed at pH 5, and maximum Cr⁶⁺ adsorption occurred at pH 3. The amount of Cr⁶⁺ adsorbed on the adsorbent increased rapidly during the first 4 h, then gradually increased, and finally reached equilibrium in 16 h. The adsorption rate of Cr³⁺ was somewhat slower than that of Cr⁶⁺. The adsorption isotherm for Cr⁶⁺ adsorption was composed of two straight lines, suggesting that the adsorbent could not practically reduce the concentration of Cr⁶⁺ in solution below 1.6 mg Cr/L Column experiments using larch leaf packing suggested that the practical operation could be controlled by monitoring the effluent pH.  相似文献   

An enzyme-linked immunosorbent assay for the detection of Clostridium perfringens enterotoxin antibody.   总被引：1，自引：1，他引：0       下载免费PDF全文

L Niilo  H J Cho 《Canadian journal of veterinary research》1984,48(1):111-112

An enzyme-linked immunosorbent assay (ELISA) was adapted to test serum antibody to enterotoxin of Clostridium perfringens type A. The test was evaluated using sheep, calf and guinea pig sera and compared with passive hemagglutination and immunodiffusion tests. The ELISA was found to be more sensitive than the other two tests and was completely free from nonspecific reactions. The method was considered to be technically advantageous and suitable for semiautomated procedures.  相似文献   

Diagnostic specificity, sensitivity and cross-reactivity of an enzyme-linked immunosorbent assay for the detection of antibody against Leptospira interrogans serovars pomona, sejroe and hardjo in cattle.   总被引：2，自引：2，他引：0       下载免费PDF全文

H J Cho  S P Gale  S A Masri    K L Malkin 《Canadian journal of veterinary research》1989,53(3):285-289

Outer sheath antigen was prepared from Leptospira interrogans serovars pomona, sejroe and hardjo by treating the organisms with 1.0M NaC1 followed by 0.04% sodium dodecyl sulfate (SDS). Sodium dodecyl sulfate was removed from the SDS-protein complexes by the extraction of dodecyl sulfate anions as ion pairs with triethylammonium cations into an organic solvent. The outer sheath antigen was recovered from the organic solvent as a precipitate and used as the source of leptospiral enzyme-linked immunosorbent assay (ELISA) antigen. Utilizing this antigen, ELISA was adapted to detect bovine serum antibody to L. interrogans serovars pomona, sejroe and hardjo. The specificity of this assay in 344 bovine sera, which were negative in the microscopic agglutination test (MAT) for seven serovars, was 99.4%. In sera from 37 and 87 cattle which revealed MAT titers greater than or equal to 1:50 for L. interrogans serovars pomona and sejroe, the relative sensitivity of the test was 100%. The ELISA also showed a considerable degree of low level cross-reactivity with other serovars. Sixty-six (75.9%) out of 87 bovine sera which were MAT-positive (MAT titer of greater than or equal to 1:50) with serovars sejroe and hardjo only were ELISA positive with heterologous pomona antigen; 16 (43.2%) and six 16.2%) out of 37 bovine sera which were MAT positive MAT titer of greater than or equal to 1:50) with serovar pomona only were ELISA positive with heterologous sejroe and hardjo ELISA antigen respectively.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Adenocarcinoma in the nasal cavity and brain of a dog     

D Y Cho  R J Bahr  H W Leipold 《Journal of the American Veterinary Medical Association》1974,165(4):350-351

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Intracranial squamous cell carcinoma in a cow     

D H Zeman  D Y Cho 《The Cornell veterinarian》1986,76(3):236-240

An exophthalmic right eye was surgically removed from a nine year old Holstein cow. Subsequent post mortem examination revealed a large intracranial mass at the base of the brain which was diagnosed as a squamous cell carcinoma. The orbital and sphenoid bones were intact and it is believed that the neoplasm entered the cranial cavity from the orbit via the foramen orbitorotundum. Both intracranial squamous cell carcinoma and extension of orbital neoplasia through foramina are rare.  相似文献   

Evolution of a microbial acetyltransferase for modification of glyphosate: a novel tolerance strategy   总被引：6，自引：0，他引：6  

Siehl DL  Castle LA  Gorton R  Chen YH  Bertain S  Cho HJ  Keenan R  Liu D  Lassner MW 《Pest management science》2005,61(3):235-240

N-Acetylation is a modification of glyphosate that could potentially be used in transgenic crops, given a suitable acetyltransferase. Weak enzymatic activity (k(cat) = 5 min(-1), K(M) = 1 mM) for N-acetylation of glyphosate was discovered in several strains of Bacillus licheniformis (Weigmann) Chester by screening a microbial collection with a mass spectrometric assay. The parental enzyme conferred no tolerance to glyphosate in any host when expressed as a transgene. Eleven iterations of DNA shuffling resulted in a 7000-fold improvement in catalytic efficiency (k(cat)/K(M)), sufficient for conferring robust tolerance to field rates of glyphosate in transgenic tobacco and maize. In terms of k(cat)/K(M), the native enzyme exhibited weak activity (4-450% of that with glyphosate) with seven of the common amino acids. Evolution of the enzyme towards an improved k(cat)/K(M) for glyphosate resulted in increased activity toward aspartate (40-fold improved k(cat)), but activity with serine and phosphoserine almost completely vanished. No activity was observed among a broad sampling of nucleotides and antibiotics. Improved catalysis with glyphosate coincided with increased thermal stability.  相似文献