Improvements in somatic embryogenesis protocol in Feijoa (Acca sellowiana (Berg) Burret): Induction,conversion and synthetic seeds     

Gabriela Claudia Cangahuala-Inocente  Lírio Luiz Dal Vesco  Douglas Steinmacher  Antonio Carlos Torres  Miguel Pedro Guerra 《Scientia Horticulturae》2007

Pineapple guava (Acca sellowiana) syn. Feijoa sellowiana, a Brazilian indigenous Myrtaceae is under domestication in South Brazil. Previous works showed that this species is responsive to somatic embryogenesis and recalcitrant to conventional methods of clonal propagation. In the present work it was evaluated the role of components of culture medium in the induction and development of somatic embryos. The technology of synthetic seeds was also evaluated. Zygotic embryos were inoculated in LPm medium supplemented with 8 mM glutamic acid and 8 mM l-glutamine, 2,4-dichlophenoxiacetic acid (20 μM) and myo-inositol. For conversion of somatic embryos and synthetic seeds it was tested the effect of 6-benzylaminopurine and gibberellic acid combined or not with activated charcoal. The highest values for embryogenetic induction (100%) and number of somatic embryos/explant (113) were observed in the LPm medium supplemented with Glu (8 mM), and 2,4-D. The culture medium supplemented with BA (0.5 μM) and GA₃ (1 μM) and activated charcoal (1.5 g L⁻¹) enhanced the conversion of somatic embryos to plantlets. Pre-germinated somatic embryos encapsulated in sodium alginate with BA (0.5 μM) and GA₃ (1 μM) developed radicles. The use of synthetic seed was a requisite for the survival of plantlets.  相似文献   

Effect of plant growth regulators on floral differentiation and seed production in Jojoba (Simmondsia chinensis (Link) Schneider)     

Loreto Prat  Claudia Botti  Thomas Fichet 《Industrial Crops and Products》2008,27(1):44-49

The effect of foliar application of cytokinin (benzyl-adenine, 150 mg L⁻¹) and gibberellin (GA₄ + GA₇, 150 mg L⁻¹) on growth and flower development of 5-year-old plants of two jojoba clones was studied. The plant growth regulators were applied on October 5, 1999 (spring) and the plants were evaluated 120, 240 and 360 days after application. Shoot length, total number of nodes and number of nodes with branching were statistically different between clones but not between the growth regulator treatments.The total number of flowers on both clones was significantly increased by treatment with benzyl-adenine (BA) and significantly reduced by treatment with gibberellin. The seed yields, evaluated 180 days after application, were not statistically different from the control due to an increase in flower abortion. One clone treated with gibberellin showed a significant decrease in number and weight of seeds, the other did not.Histology of axillary buds revealed that BA application on one clone (4.11.32) enlarged the flower meristem, differentiating multiple flower production.  相似文献   

Reinvestigation of the proteolytically active components of Bromelia pinguin fruit     

Payrol JA  Obregón WD  Natalucci CL  Caffini NO 《Fitoterapia》2005,76(6):540-548

Pinguinain is the name given to a proteolytic enzyme preparation obtained from Bromelia pinguin fruits that has been scarcely studied. The present paper deals on the reexamination of the proteases present in fruits of B. pinguin grown in Cienfuegos, Cuba. The preparation (partially purified pinguinain, PPP) showed the main characteristics of the cysteine proteases, i.e., optimum pH within alkaline range (pH 7.2-8.8), inhibition of proteolytic activity by thiol blocking reagents, which is usually reverted by addition of cysteine, a remarkable thermal stability and notable stability at high ionic strength values. Isoelectric focusing and zymogram of PPP revealed the presence of several proteolytic components between pI 4.6 and 8.1. Preliminary peptidase purification by cationic exchange chromatography showed the presence of two main proteolytic fractions with molecular masses of approximately 20.0 kDa, according to SDS-PAGE.  相似文献   

Nonthymoma‐associated exfoliative dermatitis in 18 cats          下载免费PDF全文

Monika Linek  Silvia Rüfenacht  Chiara Brachelente  Claudia von Tscharner  Claude Favrot  Sylvia Wilhelm  Claudia Nett  Ralf S. Mueller  Ursula Mayer  Monika Welle 《Veterinary dermatology》2015,26(1):40-e13

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Investigation of biofilm production and icaA and icaD genes in Staphylococcus aureus isolated from heifers and cows with mastitis          下载免费PDF全文

Lívia Castelani  Lucas Eduardo Pilon  Thamires Martins  Claudia Rodrigues Pozzi  Juliana Rodrigues Pozzi Arcaro 《Animal Science Journal》2015,86(3):340-344

Biofilm formation and antimicrobial resistance of Staphylococcus aureus are important virulence factors in cases of mastitis in dairy cows. However, few studies have investigated mastitis strains isolated from heifers. Within this context, the objective of the present study was to investigate biofilm formation on Congo red agar, the presence of the icaA and icaD genes by polymerase chain reaction (PCR), and the percentage of in vitro antimicrobial resistance of 110 S. aureus isolates from mammary gland secretions of heifers and cows with mastitis. PCR detected the icaA and icaD genes in 98% and 100% of isolates, respectively. However, only 55.5% of all isolates produced a biofilm on Congo red agar. Antimicrobial susceptibility testing revealed that 47.0% of isolates from heifers and 70.4% of isolates from cows were resistant to at least one of the antimicrobial agents tested. Resistance to penicillin and/or ampicillin was the most frequent (44.5%). These results indicate the need to implement prophylactic and control measures of mastitis for heifers. Heifers and cows can carry resistant strains with the capacity of biofilm production, a fact representing a threat to public health and animal well‐being and generating losses to dairy farmers.  相似文献   

Ocular alkaline injury in four dogs – presentation,treatment, and follow‐up – a case series          下载免费PDF全文

Claudia Busse  Claudia Hartley  Christiane Kafarnik  Mauro Pivetta 《Veterinary ophthalmology》2015,18(2):127-134

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Distribution of flavonoids and furocoumarins in juices from cultivars of Citrus bergamia Risso     

Gattuso G  Barreca D  Caristi C  Gargiulli C  Leuzzi U 《Journal of agricultural and food chemistry》2007,55(24):9921-9927

HPLC separation of flavonoids and furocoumarins in the crude juices of three cultivars of Citrus bergamia Risso ("Castagnaro", "Fantastico", and "Femminello") was carried out on a C18 reversed phase column. The analysis was performed in a single run using a DAD detector coupled with an ESI-MS-MS source. Two furocoumarins (bergapten and bergamottin) were detected and quantified simultaneously with the sixteen flavonoid components previously found in industrial bergamot juice. Full characterization of the furocoumarins was performed by (1)H NMR analysis on samples separated by means of preparative HPLC. The free-radical scavenging ability of cultivar juices was assessed by using DPPH radical. The data presented show that the "Femminello" cultivar, even though it is the least common of the three, is by far the richest in health-promoting bioactive compounds (both flavonoids and furocoumarins). Given the range of applications of furocoumarins, the preparative separation described herein is proposed as a simple and rapid method to obtain this class of compounds in good yield from crude juice.  相似文献   

Enhancing Pinus pinea cone production by grafting in a non-native habitat     

Loewe-Muñoz  Verónica  Del Río  Rodrigo  Delard  Claudia  Balzarini  Mónica 《New Forests》2022,53(1):37-55

New Forests - Pinus pinea is an interesting species for its valued pine nuts. Despite the high demand for this nut, the species is mostly harvested from natural forests, because the time elapsed...  相似文献   

Mini‐cutting technique application in Corymbia and Eucalyptus: effects of mini‐tunnel use across seasons of the year     

de Lima  Marllos Santos  Araujo  Maristela Machado  Berghetti  Álvaro Luís Pasquetti  Aimi  Suelen Carpenedo  Costella  Claudia  Griebeler  Adriana Maria  Somavilla  Luiza Michelon  dos Santos  Osmarino Pires  dos Reis Teixeira Valente  Brígida Maria 《New Forests》2022,53(1):161-179

New Forests - The forestry sector has been able to sustainably satisfy the increasing global demand for forest products, thereby positively impacting the economy, while mitigating climate change....  相似文献   

Fish feed can show genotoxic damage     

Ota  Erika do Carmo  Cardoso  Claudia Andrea Lima  Inoue  Luis Antonio Kioshi Aoki  Zanon  Ricardo Basso  Silva  Tarcila Souza de Castro 《Fish physiology and biochemistry》2022,48(3):735-748

Fish Physiology and Biochemistry - The present study was carried out to evaluate the genotoxic potential of nutritional quality of feed, using erythrocytic nuclear abnormalities assay in Nile...  相似文献