Liquid Swine Manure Can Kill Verticillium dahliae Microsclerotia in Soil by Volatile Fatty Acid, Nitrous Acid, and Ammonia Toxicity     

Conn KL  Tenuta M  Lazarovits G 《Phytopathology》2005,95(1):28-35

ABSTRACT In previous studies, liquid swine manure (LSM) was sometimes shown to reduce Verticillium wilt of potato caused by Verticillium dahliae. We also observed that microsclerotia of this fungus died within 1 day, or between 3 and 6 weeks, after addition of LSM to some acid soils and within 1 week in some alkaline soils. In this study, we demonstrated that a volatile fatty acid (VFA) mixture with an identical concentration of VFAs as that found in an effective LSM reduced germination in an acid soil (pH 5.1) to the same extent as the LSM after 1 day of exposure. Germination was reduced by 45, 75, and 90% in the 10, 20, and 40% ([wt/wt] soil moisture) treatments, respectively, with the latter being equivalent to an application of 80 hl/ha. Addition to this acid soil of 19 LSMs (30% [wt/wt] soil moisture) collected from different producers resulted in complete kill of microsclerotia with 12 manures. Effective manures had a total concentration of nonionized forms of VFAs in soil solution of 2.7 mM or higher. In some acid soils (pH 5.8), addition of LSM (40% [wt/wt] soil moisture) did not kill microsclerotia until 3 to 6 weeks later. Here, a reduction in viability of microsclerotia was attributed to the accumulation of 0.06 mM nitrous acid in the soil solution at 4 weeks. When an LSM was added (40% [wt/wt] soil moisture) to an alkaline soil (pH 7.9) where VFAs are not toxic, microsclerotia germination was reduced by 80% after 1 week. Here the pH increased to 8.9 and the concentration of ammonia reached 30 mM in the soil solution. An ammonium chloride solution having an equivalent concentration of ammonium as the manure was shown to have the same spectrum of toxicity as the manure in assays ranging from pH 7 to 9, both in solutions and above the solutions. At pH 9, the concentration of ammonia reached 18 mM and 100% mortality of microsclerotia occurred. Thus, in acid soils, LSM can kill microsclerotia of V. dahliae by VFA and/or nitrous acid toxicity and in alkaline soils by ammonia toxicity. In order to take advantage of these mechanisms for disease reduction, the manure chemical composition, rate of addition, and soil characteristics need to be determined for each instance of use.  相似文献   

Metabolic clearance rate of cortisol in pigs: relationship to adrenal responsiveness   总被引：1，自引：0，他引：1  

D P Hennessy  R J Conn  S S Wan 《Research in veterinary science》1986,41(3):361-364

Experiments were conducted on 12 prepuberal (18- to 20-week-old) Landrace cross Large White gilts to establish if differences in adrenal responsiveness between individuals could be explained by differences in the metabolic clearance rate (MCR) of cortisol. Pigs with the highest (n = 6) and lowest (n = 6) cortisol concentration 60 minutes after challenge with ACTH were selected from a pool of 36 commercial pigs. Tritium-labelled cortisol was infused (17 to 27 ml h-1) continuously for 120 minutes to establish 'steady state' conditions. Blood samples (10 ml) were collected at 90, 100, 110 and 120 minutes. Replicate experiments were performed on some pigs. Classification of individual pigs as high or low adrenal responders to ACTH challenge was confirmed at the end of the clearance rate experiments. The MCR of cortisol in the group classed as low adrenal responders was 59.7 +/- 7.8 litres h-1 or 1.01 litres h-1 kg-1 (n = 7) which was not significantly different from the average MCR in the group classed as high adrenal responders 60.2 +/- 5.9 litres h-1 or 1.19 litres h-1 kg-1 (n = 10). These results suggest that the repeatable differences in adrenal responsiveness to ACTH that exist between individuals within a particular strain of pig depend on differences in the rate of synthesis of cortisol in response to ACTH stimulation, rather than on differences in its rate of metabolism.  相似文献   

CERTIFIED SAFRANIN     

Conn HJ 《Science (New York, N.Y.)》1924,59(1538):556-557

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HYPHENATION OF ENGLISH COMPOUND NAMES     

Conn HJ 《Science (New York, N.Y.)》1939,89(2321):582-583

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REPOR'T OF THE SUMMER SCHOOL OF THE BROOKLYN INSTITUTE FOR THE SEASON JUST CLOSED     

Conn HW 《Science (New York, N.Y.)》1892,20(502):157-159

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Edaphic Soil Levels of Mineral Nutrients, pH, Organic Matter, and Cationic Exchange Capacity in the Geocaulosphere Associated with Potato Common Scab   总被引：1，自引：0，他引：1  

Lazarovits G  Hill J  Patterson G  Conn KL  Crump NS 《Phytopathology》2007,97(9):1071-1082

ABSTRACT In order to determine possible relationships between geocaulosphere soil properties and severity of common scab of potato caused by Streptomyces scabies, soils were collected from representative commercial potato fields in Canada: in Simcoe and Dufferin Counties, Ontario and across Prince Edward Island (PEI) in August 2004. Soils immediately adjacent to tubers were sampled and analyzed for select edaphic factors and for pathogen presence using polymerase chain reaction (PCR) tests with primers that amplify a region of the TxtA gene involved in regulating the biosynthesis of the thaxtomin toxin family. Individual tubers were assessed visually for scab severity. The relationships between soil chemical factors and disease severity were investigated for each region to detect the strongest relationships. Principal component analysis revealed a distinctive clustering of samples with respect to disease severity in PEI but not in Ontario soils. Total and percent saturation of K (%K) were the only factors found associated with high disease severity in soils from both provinces. In PEI soils, pH, Mg, Ca, Cu, and %K, %Mg, %Ca, and %Na were associated with high disease severity, whereas cation exchange capacity (CEC) and Al were correlated with low disease severity soils. In Ontario, high Mn content was strongly correlated with low disease severity soils, whereas %K and organic matter content were correlated with disease severity. Partitioning samples into presence or absence of the TxtA PCR product with corresponding high or low severity showed further significant relationships in the data. There was an excellent correlation between Streptomyces spp. presence as detected by PCR and disease severity in PEI soils; however, the relationship was not as clear in Ontario soils, where many PCR-positive soils had low disease incidence. Principal component and partial least square analysis indicated that disease severity was predicted by soil factors such as organic matter, CEC, pH, Al, %Ca, %Mg, and %K for PEI but not for Ontario soils. The data reveal that the relationship between scab severity and soil chemical components is complex and potentially soil specific.  相似文献   

INVESTIGATIONS CONCERNING IMPORTED BIOLOGICAL STAINS     

Conn HJ 《Science (New York, N.Y.)》1924,59(1528):328-331

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A REPORT ON BASIC FUCHSIN     

Conn HJ 《Science (New York, N.Y.)》1924,60(1556):387-388

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AMERICAN EOSINS     

Kornhauser SI  Mallory FW  Novy FG  Sharp LW  Conn HJ 《Science (New York, N.Y.)》1922,56(1459):689-690

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Characterization of the receptor for gonadotropin-releasing hormone in the pituitary of the African catfish,Clarias gariepinus     

R. De Leeuw  P. M. Conn  C. Van't Veer  H. J. Th. Goos  P. G. W. J. Van Oordt 《Fish physiology and biochemistry》1988,5(2):99-107

Receptors for gonadotropin-releasing hormone (GnRH) were characterized using a radioligand prepared from a superactive analog of salmon GnRH (sGnRH), D-Arg⁶-Pro⁹-sGnRH-NEt (sGnRHa). Binding of¹²⁵I-sGnRHa to catfish pituitary membrane fractions reached equilibrium after 2 h incubation at 4°C. Displacement experiments with several GnRH analogs as well as other peptides, demonstrated the specificity of¹²⁵I-sGnRHa binding. Specific binding was enhanced in the presence of the cation chelator ethylene bis (oxyethylenenitrilo) tetra-acetic acid (EGTA), indicating an inhibitory effect of cations on GnRH-receptor binding. The binding of¹²⁵I-sGnRHa to pituitary membranes was found to be saturable at radioligand concentrations of 5 nM and above. A Scatchard analysis of the saturation data suggested the presence of a single class of high-affinity binding sites (Ka=0.901±0.06×10⁹M^–1, Bmax=1678±150 fmol/mg protein). A comparative study on¹²⁵I-sGnRHa binding to pituitary membrane fractions of male and female catfish, indicated that there were no differences in binding affinity and binding capacity between both sexes. The results demonstrate the presence of specific, saturable GnRH receptors in the African catfish pituitary.  相似文献