病态池的检查和维修技术     

代德超  王敬堂 《中国沼气》2006,24(1):34-35,51

随着沼气建设的快速发展,病态池的数量也随之增多。本文就如何分辨沼气池和输气线路病态、确定病态沼气池的病态类型、查找病态池漏水漏气部位及维修病态池提出了具体的方法。  相似文献   

汽车发动机振动传感器的研制     

戴梦萍  纪永秋 《农业装备与车辆工程》2007,(8):17-19

阐述了手持式内置微型差动式电荷放大器和频率补偿电路一体化的发动机振动传感器。使用ispPAC可编程器件制作自适应频率补偿电路,展宽了频带,拓宽了手持式传感器的应用范围。实验表明该传感器具有灵敏度高、测量范围宽、温度误差小等特点。  相似文献   

Expression of cathepsin C and tumor necrosis factor-α in human coronary artery and their relationships with coronary heart disease     

ZHANG Qiong  WAN Chang-wu  YU Yan-ni  XIA-Bing  LIU Jiang-jin  ZHANG Qiao-jun  LI Zhu  WANG Cheng-fei  DAI Jia-lin  WANG Jie 《园艺学报》2021,36(12):2133-2138

AIM To explore the possible mechanism of cathepsin C （CTSC） and tumor necrosis factor-α （TNF-α） in coronary heart disease （CHD） by detecting the protein expression of CTSC and TNF-α in human coronary artery tissue. METHODS The coronary artery tissues from 52 cases of CHD and 25 cases of accidental death without any heart disease in the Forensic Judicial Expertise Center of Guizhou Medical University from October 2018 to December 2019 were collected as CHD group and control group, respectively. The coronary artery stenosis and intimal plaque formation were examined by histopathology, the protein expression of CTSC and TNF-α was determined by Western blot, and the intracellular expression of CTSC and TNF-α was analyzed by immunohistochemical staining. RESULTS The results of HE staining showed that the intima of coronary artery in control group was smooth, and no thickening or stenosis was observed. In CHD group, the intima thickened irregularly, atherosclerotic plaques formed, the intima became thinner and the lumen showed eccentric stenosis in varying degrees （P<0.05）. The results of Western blot showed that the expression of CTSC and TNF-α in CHD group was significantly higher than that in control group （P<0.05）. Immunohistochemical staining showed that both CTSC and TNF-α were expressed in the cytoplasm of foam cells, and their positive expression was significantly higher than that in control group （P<0.05）. The results of Pearson moment correlation analysis showed that there was positive correlation between the expression of CTSC and TNF-α in CHD （r²=0.743, P<0.05）. CONCLUSION The up-regulated expression of CTSC in coronary artery tissue may promote the expression of TNF-α and affect the occurrence and development of CHD.  相似文献   

生物有机肥对黄州萝卜主要经济性状和效益的影响     

卢华平  闫良  熊鑫镭  徐丽荣  蒋艳艳  代俊芬  葛长军 《蔬菜》2021,(8):26-29

为了验证生物有机肥对蔬菜作物生长的促进作用与增产效应,以黄州萝卜为试验材料,探究不同生物有机肥对黄州萝卜产量及品质等方面的影响,设置3个施肥处理:每667 m~2分别施用200 kg生物有机肥+20 kg三元复合肥;200 kg灭活生物有机肥+20 kg三元复合肥;常规施肥(每667 m~2施200 kg饼肥+20 kg三元复合肥)以及空白对照。结果表明:每667 m~2施200 kg生物有机肥+20 kg三元复合肥处理的萝卜667 m~2产量最高,为2 921.5 kg;在品质方面,萝卜含粗纤维7 g/kg,可溶性糖42 g/kg,水分92.6%;综合667 m~2效益最优,达到4 567.3元,产投比最高,为3.5。  相似文献   

《菌谱》中管窥宋代食用菌饮食文化     

代晓丽 《中国食用菌》2021,(2):126-129

我国宋代时期整体政治稳定、经济繁荣,人文发展得到空前提高,良好环境为我国饮食文化的繁荣奠定了基础。此时期的饮食文化中开始注重饮食营养的全面化,讲究食材烹饪的精细化。《菌谱》作为我国最早撰写的食用菌专著,是宋代对食用菌饮食的整理性文献。解读《菌谱》中食用菌的饮食文化,有助于通过结合食用菌饮食类别,研究宋代食用菌饮食文化的特征,这不仅可以了解当时人们的食用菌饮食习惯,更能折射出宋代食用菌文化的丰富。  相似文献   

6个枣品种枣疯病抗性与枣疯植原体迁移特性差异研究     

郝新迪  路瑾瑾  代丽珍  张铁强  王进忠  王龙  郭家洛  任争光  王建文 《果树学报》2021,(4):560-568

【目的】探究6个枣树品种枣疯病抗性差异以及枣树体内植原体迁移特性差异,为抗枣疯病枣树品种的选择利用以及枣疯病的防治提供基础数据。【方法】通过采集携带植原体的鸡心脆枣接穗,嫁接到健康枣树砧木上,采用田间调查与普通PCR技术相结合方法,比较不同品种抗枣疯病情况与植原体在不同枣树品种中迁移情况。【结果】不同枣树品种发病时间不同,发病率差异显著,蜂蜜罐枣、尜尜枣和猴头枣为抗病类型,冬枣、红螺脆枣和鸡心脆枣为感病类型。植原体在不同枣树品种中迁移速度不同,植原体在鸡心脆枣和冬枣内迁移速度最快,其后依次为蜂蜜罐枣、红螺脆枣、尜尜枣,在猴头枣内迁移速度最慢。【结论】6个枣树品种中蜂蜜罐枣、尜尜枣和猴头枣表现一定的抗病能力,初步确认了植原体在不同枣树品种体内迁移特征的差异,可为抗枣疯病品种的选择利用和枣疯病防治提供科学依据。  相似文献   

五华鸡J亚群白血病病毒env部分基因的和序列分析     

戴银  张丹俊  胡晓苗  赵瑞宏 《中国畜牧兽医》2011,(12)

本试验通过PCR技术获得了安徽省地方品种五华鸡禽白血病病毒J亚群(avian leukosis virus subgroup J,ALV-J)env部分基因序列AL V-J-env1,并将该序列与GenBank数据库中登录的8条env基因相应序列进行了比对分析.结果表明,五华鸡已经感染了J亚群禽白血病,且部分鸡个体已经发病.通过分析可见,AL V-J-env1与所比较的基因序列同源性介于94.2％～96.6％之间,说明不同毒株之间的env基因有一定变异.但与ALV J-env1同源性最高的是来自于中国ALV-J毒株的HQ425636和HM235665基因序列,且在进化树中聚集为一组,暗示它们之间亲缘关系较近,由共同的毒株进化而来.与ALV-J-env1同源性最低的是来源于马来西亚毒株的AY312965基因序列,遗传进化分析也进一步证实,两者之间亲缘关系较远.  相似文献   

东北虎γ-干扰素基因的克隆、生物信息学分析及其在毕赤酵母中的表达     

翟俊琼  吴亚江  代军威  陈安妮  周妞  王晨  杜雪晴  罗满林  陈武 《中国畜牧兽医》2021,(1)

本试验旨在通过克隆东北虎γ-干扰素(IFN-γ)基因,研究其分子特征并预测蛋白生物学功能,为后续研究干扰素抗病毒活性做前期准备。通过RT-PCR从ConA诱导过的东北虎血淋巴细胞中扩增东北虎IFN-γ基因并测序,应用生物信息学方法进行序列分析。结果表明:东北虎IFN-γ编码区由504个核苷酸组成,共编码167个氨基酸,蛋白相对分子质量为19.59 ku,等电点为9.03,所编码的蛋白为碱性亲水性蛋白,其中前23个氨基酸可能为信号肽,IFN-γ编码蛋白保守结构域为IFN-γ超家族,且存在跨膜结构,其中1-6位氨基酸为胞内区域,7-28位氨基酸为跨膜区域,29-167位氨基酸为胞外区域;IFN-γ编码蛋白二级结构主要以α-螺旋(58.08%)和无规则卷曲(33.53%)为主,存在5个潜在的B细胞抗原表位,3个潜在的N-糖基化位点;分子进化分析显示,东北虎IFN-γ与GenBank上发表的东北虎、非洲狮、金钱豹、美洲狮、猎豹、家猫、加拿大猞猁、野猪等的核苷酸相似性为80.4%~99.8%,氨基酸相似性为70.5%~100%,东北虎IFN-γ与非洲狮、金钱豹亲缘关系最近,美洲狮、猎豹、家猫、猞猁次之,野猪最远。通过合成改造后的东北虎IFN-γ基因,构建能表达IFN-γ蛋白的重组质粒pPIC9K-IFN-γ,将其导入高效表达系统-毕赤酵母中进行诱导表达,经SDS-PAGE分析,表达蛋白的分子量约17.8 ku,与预期大小相符,表明东北虎IFN-γ成功表达。  相似文献   

巧用AutoCAD软件的“三维旋转”命令绘制实体课件     

宋晓英 《计算机与农业》2011,(3):116-118

巧妙地应用AutoCAD软件的＂三维旋转＂命令,可以方便快捷地绘制带有倾斜结构及局部剖结构的实体课件,并用两个实例说明了具体的应用方法。  相似文献   

Effects of pirenzepine on expression of MMP-2 and TIMP-2 in stroma of sclera in chick myopic eyes     

DAI Shu-zhen  ZHANG Li  WANG Li-ya  ZENG Jun-wen 《园艺学报》2012,28(8):1494-1499

AIM:To observe the effect of intravitreal injection of pirenzepine on form-deprivation myopia in chicks. METHODS:Forty-one day old chicks were randomly divided into 4 groups:normal control group, form deprivation group, vehicle control group and pirenzepine injection group. The right eyes of all chicks were used as experimental eyes. The deprived eyes in vehicle control group and pirenzepine injection group received daily intravitreal injection of vehicle control solution(0.01 mol/L PBS) and pirenzepine(1%in PBS), respectively. Optical examinations such as refraction, axial length and equatorial diameter were made at the end of the 5th day. The mRNA and protein levels of matrix metalloproteinase 2(MMP-2) and tissue inhibitor of metalloproteinase-2(TIMP-2), and the activity of MMP-2 were detected by RT-PCR, Western blotting and zymography analysis,respectively. RESULTS:Refraction, axial length and equatorial diameter of the eyes in pirenzepine injection group were significantly lower than those in form deprivation group and vehicle control group(P<0.05), but those were higher(P<0.05) and the eyes were relatively myopic as compared with normal control group. The mRNA expression, protein le vels and activity of MMP-2 in pirenzepine group were significantly higher than those in normal control group(P<0.01), and were significantly lower than those in form deprivation group and vehicle control group(P<0.01, P<0.01). The mRNA and protein levels of TIMP-2 in pirenzepine group were significantly lower than those in normal control group(P<0.01), and was significantly higher than those in form deprivation group and vehicle control group(P<0.01, P<0.01). CONCLUSION:Intravitreal injection of pirenzepine may partly prevent form-deprivation myopia by modulating the expression of MMP-2 and TIMP-2 in the fibrous layer of sclera.  相似文献