BVDV in British alpacas     

Barnett J  Twomey DF  Millar MF  Bell S  Bradshaw J  Higgins RJ  Scholes SF  Errington J  Bromage GG  Oxenham GJ 《The Veterinary record》2008,162(24):795

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Plant Nematode Control A.G. Whitehead     

Ursula Twomey 《Integrated Pest Management Reviews》1999,4(4):317-318

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Automated aerosol puffers effectively deliver 1‐OCTEN‐3‐OL,an oviposition antagonist useful against spotted‐wing drosophila     

Dara G Stockton  Anna K Wallingford  Dong H Cha  Gregory M Loeb 《Pest management science》2021,77(1):389-396

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Ute Russet: An oblong Russet potato cultivar with resistance to leafroll net necrosis andSclerotium rolfsii     

D. G. Holm  J. A. Twomey  M. Workman 《American Journal of Potato Research》1987,64(10):559-562

Ute Russet is an oblong, smooth, fresh market potato with a dark net. It emerges slowly, produces an erect, medium sized vine, and is late maturing. Ute Russet is resistant toSclerotium rolfsii Sacc, and leafroll net necrosis, and moderately resistant toVerticillium wilt and scab. Its total and U.S. No. 1 yield potential is comparable to Centennial Russet in the San Luis Valley of Colorado and greater than Centennial Russet in Kern County, California.  相似文献   

Immunophenotyping of lymphosarcoma in South American camelids on six British premises     

Twomey DF  Barlow AM  Hemsley S 《Veterinary journal (London, England : 1997)》2008,175(1):133-135

Six cases of lymphosarcoma (LSA) in South American camelids (SACs) were selected from submissions to a diagnostic laboratory network servicing England and Wales. Immunophenotyping was carried out using anti-human CD3 and anti-human CD5 for T-cells; and anti-human CD79a and anti-human CD79b for B-cells/plasma cells. On the basis of labelling with mainly anti-CD3, four of the tumours were classified as T-cell tumours. One case was labelled with anti-CD79a and anti-CD79b, and was classified as a B-cell tumour. In the other case the majority of cells were labelled with anti-CD3, anti-CD79a and anti-CD79b, and was classified as a mixed T- and B-cell tumour. To the authors' knowledge this is the first reported attempt at immunophenotyping LSA in SACs on British premises and is only the second time that a presumptive mixed T- and B-cell LSA has been reported in alpacas and the veterinary literature in general.  相似文献   

Cryptosporidiosis in two alpaca (Lama pacos) holdings in the South-West of England     

Twomey DF  Barlow AM  Bell S  Chalmers RM  Elwin K  Giles M  Higgins RJ  Robinson G  Stringer RM 《Veterinary journal (London, England : 1997)》2008,175(3):419-422

Cryptosporidiosis was investigated on two alpaca (Lama pacos) holdings in the South-West of England. Diagnosis was initially confirmed in a cria with diarrhoea from each holding. Cohort faeces samples were subsequently collected and examined for presence of Cryptosporidium oocysts by immunofluorescence microscopy. On the first holding, 30 samples (24 adults, 6 crias) were tested, and oocysts were detected in three of the cria samples but in none of the adults. On the second holding, 14 floor faeces samples representing apparently healthy crias and one faeces sample from a cria with diarrhoea were collected. Oocysts were detected in four of the "healthy" faeces samples and the sample of diarrhoeic faeces. All isolates were confirmed as Cryptosporidium parvum using polymerase chain reaction restriction fragment length polymorphism of the cryptosporidium oocyst wall protein (COWP) and ssu rRNA genes. Sequence analysis of a 741bp region of ssu rDNA was carried out on nine of these and revealed high sequence homology with previously reported C. parvum isolates. This investigation highlights the possibility of alpaca crias subclinically shedding oocysts, which has implications for epidemiology and transmission in animals as well as raising zoonotic concerns for human contacts. Gene sequencing of UK isolates from South American camelids is also described for the first time.  相似文献   

Phenotypic characterisation and 16S rRNA sequence analysis of veterinary isolates of Streptococcus pluranimalium     

Twomey DF  Carson T  Foster G  Koylass MS  Whatmore AM 《Veterinary journal (London, England : 1997)》2012,192(2):236-238

Forty-two isolates of Streptococcus pluranimalium were identified from cattle (n=38), sheep (n=2), an alpaca (n=1) and a pheasant (n=1) in the United Kingdom. The isolates were confirmed as S. pluranimalium by 16S rRNA sequence analysis but could not be differentiated reliably from Streptococcus acidominimus by phenotypic characterisation using commercial kits routinely used in veterinary laboratories. The alanyl-phenylalanyl-proline arylamidase reaction could be used to differentiate S. pluranimalium (positive) from Aerococcus urinae (negative).  相似文献   

Controlling tuberculosis in a llama (Lama glama) herd using clinical signs, tuberculin skin testing and serology     

Twomey DF  Collins R  Cranwell MP  Crawshaw TR  Higgins RJ  Dean GS  Vordermeier HM  Hollingdale A  de la Rua-Domenech R 《Veterinary journal (London, England : 1997)》2012,192(2):246-248

An outbreak of tuberculosis (TB), caused by Mycobacterium bovis, was investigated in a small herd of llamas (Lama glama). Based on three ante-mortem diagnostic methods (clinical signs, tuberculin skin test reactions, and 'Rapid Test' serology), 12 llamas were selected for examination post-mortem. Grossly visible lesions suspicious of TB were observed in eight animals, four of which had exhibited clinical signs, one was a skin test 'reactor', and three had been seropositive. M. bovis was isolated from seven of these eight animals. Clinical signs combined with serology were found to be useful in identifying infected animals, but tuberculin skin testing had limited negative predictive value as four llamas that were subsequently confirmed as infected were not detected using this assay.  相似文献   

Ovarian cyst in a maiden heifer     

Twomey DF 《The Veterinary record》1999,144(2):56

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Comparative efficacy of dry-cow treatment regimens against Staphylococcus aureus     

Pankey JW  Barker RM  Twomey A  Duirs G 《New Zealand veterinary journal》1982,30(1-2):13-15

Four dry-cow treatment regimens were evaluated against Staphylococcus aureus intramammary infections: 1) high persistency product at drying off and low persistency product 1 to 3 days prepartum; 2) high persistency product at drying off; 3) low persistency product 1 to 3 days prepartum; 4) untreated controls. Treatment 1 was no more efficacious (64.4%) than Treatment 2 (61.3%). Both treatments were significantly different from spontaneous recovery observed in Treatment 4 (41.2%), but not significantly different from Treatment 3 (46.9%). Dry-cow therapy reduced new S. aureus intramammary infections during the dry period by half. Prepartum treatment eliminated more than 90% of new Streptococcus uberis infections.  相似文献