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21.
22.
OBSERVATIONS ON THE GROUND BEETLE FAUNA OF BRASSICA CROPS 总被引:1,自引:1,他引:0
23.
Thirty-five isolates of Pasteurella multocida from the vagina and respiratory tract of sheep were compared by analysing their capsular polysaccharide types and outer membrane protein profiles. The phylogenetic relationships of selected isolates with respect to reference strains of P. multocida were also determined by comparative 16S rRNA sequence analysis. Three capsular types, A, D and F, and three major outer membrane protein types were identified, and there were four different combinations of these characteristics which probably marked four individual clones of P. multocida. Strains representing three of these clones were recovered from cases of ovine pneumonia, whereas isolates of the fourth clone were associated exclusively with the vagina of healthy ewes and the liver of a dead septicaemic lamb on the same farm. Analysis of the 16S rRNA sequences showed that there was 100 per cent identity between representative pneumonic isolates and reference strains of P. multocida subspecies galliseptica and P. multocida subspecies multocida. The 16S rRNA genes of representative vaginal and liver isolates from the same farm were identical but differed from the other strains at one nucleotide position, providing strong evidence that the vaginal and liver isolates represent a distinct subpopulation of P. multocida. 相似文献
24.
Cathepsin K and cathepsin B were immunolocalised in equine osteoclasts (OC s) present in ex vivo cartilage/subchondral bone samples. Samples were obtained post mortem from the lateral trochlear ridge (LTR) of six horses and ponies aged between 303 days gestation to 8 months. Strong expression of cathepsin K was detected in OC s, particularly those located at the osteochondral junction, apparently involved in the resorption of calcified cartilage. Cathepsin K expression was also detected in hypertrophic chondrocytes and in the endothelial cells of some blood vessels penetrating the hypertrophic zone of cartilage. By contrast, cathepsin B was either absent or present at very low levels in OC s.Osteoclast-like cells (OCL s) were generated in vitro from bone marrow (BM), obtained from the femurs of one horse and two ponies. High levels of cathepsin K activity but only very low levels of cathepsin B activity were demonstrated in OCL s using fluorogenic substrates for these enzymes. The cathepsin K activity could be blocked by the general cysteine proteinase inhibitor, E-64, but not by the cathepsin B inhibitor, CA-074Me. The cathepsin B activity was completely blocked by both CA-074Me and E-64. Taken together, these results suggest that cathepsin K is more important than cathepsin B in the osteoclastic resorption of bone and calcified cartilage of developing equine long bones. Given the apparent importance of cathepsin K in equine endochondral ossification further investigation into the possibility that abnormal expression of this enzyme is involved in the pathogenesis of equine developmental orthopaedic disease is warranted. 相似文献
25.
26.
Rusbridge C MacSweeny JE Davies JV Chandler K Fitzmaurice SN Dennis R Cappello R Wheeler SJ 《Journal of the American Animal Hospital Association》2000,36(1):34-41
Syringohydromyelia secondary to foramen magnum overcrowding is described in seven Cavalier King Charles spaniels. Clinical signs were consistent with a central spinal cord lesion. The most common signs were persistent scratching at the shoulder region with apparent neck, thoracic limb, or ear pain and thoracic limb lower motor neuron deficits. The diagnosis was made by magnetic resonance imaging. The syringohydromyelia is postulated to be a consequence of an occipital bone malformation resulting in a small caudal fossa and cerebellar herniation. Clinical signs improved but did not completely resolve when the dogs received treatment with corticosteroids or nonsteroidal anti-inflammatory drugs. 相似文献
27.
28.
Previous experiments have shown that mixed or conjoint grazing, when two or more animal species are grazed together, can lead to improved performance of one or more of the species and a higher total output per unit area. Sequential grazing systems, when different animal species graze an area in succession, also have the potential to improve productivity. This experiment directly compared these two approaches for integrating the grazing of cattle and sheep when pastured on improved permanent pasture. Four treatments were compared: 1) sheep only from May to October (S/S); 2) cattle only from May to July followed by sheep only from August to October (C/S); 3) cattle and sheep from May to July, sheep only for the rest of the growing season (C + S/S); and 4) cattle and sheep from May to October (C + S/C + S). Each treatment was replicated three times. From May until weaning at the end of July the plots were grazed by steers and ewes and lambs, and from weaning until October by steers and lambs. Sward heights were maintained at 6 cm using a “put and take” stocking system. Animal performance and sward composition data were collected during three growing seasons, 2001–2003. During the pre-weaning period lambs grazing plots which were grazed only by sheep had significantly poorer growth rates than those grazing plots where there was mixed grazing. Lambs grazing the sheep only plots also had lower liveweight gain during the post-weaning period, with the highest growth rates being recorded on the C + S/C + S treatment. Carrying capacity and total liveweight gain per unit area post-weaning were also consistently higher for the C + S/C + S treatment, while carrying capacity pre-weaning was highest for the C/S treatment. Measurements of clover content in the grazed horizon and botanical separation of material from quadrat cuts indicated that even under relatively controlled conditions choice of grazing system can influence sward composition. However, there was no clear link between any single sward change and the growth of the animals, indicating that the improved performance of cattle and sheep under mixed grazing is the result of a combination of factors, rather than a response to a particular sward parameter. 相似文献
29.
Comparison of the Sensitivity of Landscape-fire-succession Models to Variation in Terrain, Fuel Pattern, Climate and Weather 总被引:1,自引:0,他引:1
Geoffrey J. Cary Robert E. Keane Robert H. Gardner Sandra Lavorel Mike D. Flannigan Ian D. Davies Chao Li James M. Lenihan T. Scott Rupp Florent Mouillot 《Landscape Ecology》2006,21(1):121-137
The purpose of this study was to compare the sensitivity of modelled area burned to environmental factors across a range of
independently-developed landscape-fire-succession models. The sensitivity of area burned to variation in four factors, namely
terrain (flat, undulating and mountainous), fuel pattern (finely and coarsely clumped), climate (observed, warmer & wetter,
and warmer & drier) and weather (year-to-year variability) was determined for four existing landscape-fire-succession models
(EMBYR, FIRESCAPE, LANDSUM and SEM-LAND) and a new model implemented in the LAMOS modelling shell (LAMOS(DS)). Sensitivity
was measured as the variance in area burned explained by each of the four factors, and all of the interactions amongst them,
in a standard generalised linear modelling analysis. Modelled area burned was most sensitive to climate and variation in weather,
with four models sensitive to each of these factors and three models sensitive to their interaction. Models generally exhibited
a trend of increasing area burned from observed, through warmer and wetter, to warmer and drier climates with a 23-fold increase
in area burned, on average, from the observed to the warmer, drier climate. Area burned was sensitive to terrain for FIRESCAPE
and fuel pattern for EMBYR. These results demonstrate that the models are generally more sensitive to variation in climate
and weather as compared with terrain complexity and fuel pattern, although the sensitivity to these latter factors in a small
number of models demonstrates the importance of representing key processes. The models that represented fire ignition and
spread in a relatively complex fashion were more sensitive to changes in all four factors because they explicitly simulate
the processes that link these factors to area burned.
The US Government's and the Canadian Government's right to retain a non-exclusive, royalty-free license is acknowledged 相似文献
30.
Are hedgerows effective corridors between fragments of woodland habitat? An evidence-based approach 总被引:1,自引:1,他引:1
Anthropogenic modification of the countryside has resulted in much of the landscape consisting of fragments of once continuous
habitat. Increasing habitat connectivity at the landscape-scale has a vital role to play in the conservation of species restricted
to such remnant patches, especially as species may attempt to track zones of habitat that satisfy their niche requirements
as the climate changes. Conservation policies and management strategies frequently advocate corridor creation as one approach
to restore connectivity and to facilitate species movements through the landscape. Here we examine the utility of hedgerows
as corridors between woodland habitat patches using rigorous systematic review methodology. Systematic searching yielded 26
studies which satisfied the review inclusion criteria. The empirical evidence currently available is insufficient to evaluate
the effectiveness of hedgerow corridors as a conservation tool to promote the population viability of woodland fauna. However,
the studies did provide anecdotal evidence of positive local population effects and indicated that some species use hedgerows
as movement conduits. More replicated and controlled field investigations or long-term monitoring are required in order to
allow practitioners and policy makers to make better informed decisions about hedgerow corridor creation and preservation.
The benefits of such corridors in regard to increasing habitat connectivity remain equivocal, and the role of corridors in
mitigating the effects of climate change at the landscape-scale is even less well understood. 相似文献