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We used spectrometry to study variation in the contents of minor (B, Cu, Fe, Mn and Zn) and major essential elements (Ca, K, Mg, Na and P) in the muscle tissue and digestive glands of Octopus vulgaris after 1, 2, 4 or 8 days of starvation and in fed controls. The contents of the major essential elements remained constant during 8 days of starvation. Na was the principal element found in both tissues, followed by K, P and Mg. The contents of the minor essential elements were higher in the digestive gland than in the muscle tissue, with increased contents of Fe, Mn and Zn (p < .05) during starvation, confirming the high capacity of this organ to accumulate trace minerals. In muscle tissues, B, Fe and Mn contents were lower and Cu and Zn contents higher, in starved animals than in fed animals (p < .05). Significant negative correlations between the digestive gland index and the contents of Cu, Fe and Zn suggested that the loss of weight of the digestive gland during starvation is responsible for this increase in mineral concentration. This and further information on variation in mineral content will help to optimize performance of diets formulated for the octopus.  相似文献   
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An in vitro model designed to assess protein bioavailability in diets for growing Octopus was developed. The model required a previous assessment of some functional features of protein digestion in this species like the main producing organs, optimum pH for activity and total production per g tissue. The main producing organs identified were the salivary glands and the hepatopancreas (HP), being optimum pH for protease activity quite different in both organs (mostly alkaline in the posterior salivary glands and acid in the HP). In spite of the high‐specific protease activity measured in the salivary glands, a major role of the HP in protein hydrolysis is suggested due to the much bigger size of this viscera. All this information was used as a basis to develop an in vitro two‐step hydrolysis process, which simulated protein hydrolysis performed by these two organs using the Octopus enzymes. The assay was used to evaluate differences in amino acid bioavailability from several protein sources (casein, gelatin, fish meal, squid meal and krill meal) that could be used as feed ingredients for this species. As significant differences were detected both in total amount and in rate of release of the amino acids from such proteins, the model is proposed as a complementary tool in the selection and nutritional evaluation of protein ingredients to be used in diets for Octopus.  相似文献   
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We tested the prediction that climate-driven changes might alter bird species composition in reserves of the Cerrado region of Brazil. First, we modelled the current distributions and the potential future projections of 38 endemic or rare bird species. We used eight modelling techniques within the BIOMOD computational framework in an ensemble-forecasting approach to reach a consensus scenario. Then we compared current and future (2046-2060) distributions under different scenarios (reserve size and bird dispersal) with the current Brazilian reserve system to assess the adequacy of protection (representation) of each species and detect gaps in their protection. Finally, to identify areas with high probability of occurrence of several species, we calculated cumulative climatic suitability of all 38 species for both current and future scenarios. None of the 38 species is covered under any current or future scenarios, revealing that the current reserve system is highly inefficient in conserving the analyzed bird species. The implementation of new reserves to cover species in current and future climate scenarios is recommended in areas in the south-eastern part of the Cerrado region and in the mountains of east Brazil. Due to the already high land use of the southeast region of the Cerrado, the application of non-traditional conservation measures should be evaluated.  相似文献   
27.
In this work, we study the variations in concentration (mg 100 g?1 dry weight) and total content (mg individual?1) of different lipid classes in muscle and the digestive gland of Octopus vulgaris during an 8‐day starvation period. In all the samples analysed, polar lipids (PL) predominated in muscle (267.3–337.2 mg 100 g?1) compared with neutral lipids (66.9–104.7 mg 100 g?1). A significant positive tendency was observed in muscle for the content and concentration of monoglycerides as a consequence of starvation (P < 0.05). Furthermore, a higher cholesterol (CHO) content was detected in this tissue after the fourth day of starvation compared with the fed animals (P < 0.05). Neutral lipids predominated in the digestive gland (12 958–14 151 mg 100 g?1) compared with PL (3157–6517 mg 100 g?1), with triglycerides, free fatty acids and monoglycerides being the major lipid classes. The concentration of PL and monoglycerides showed a positive trend with starvation, while the triacylglycerol showed a negative tendency (P < 0.05). The results suggest that triglycerides and CHO are transported simultaneously from the digestive gland to the muscular tissues during starvation and the preferential use of PL and CHO during growth phases. It was estimated that lipids contributed 26% of the energy costs of the animals during starvation, mainly in the form of triglycerides from the digestive gland.  相似文献   
28.
Growth, feed efficiency and proximate and lipid class composition of subadults Octopus vulgaris (788 ± 133 g; 18.5°C) fed formulated diets of low lipid (LL: 8 g kg?1) and high lipid levels (HL: 84 g kg?1) and each one of these with three different levels of glutamate supplementation (0, 5 and 20 g kg?1) were compared. All the animals accepted the diets with a survival of 100%. The addition of glutamate did not stimulate feeding rates in any of the assays (2.48–2.64 and 1.86–2.01%Body weight day?1 for LL and HL, respectively; P > 0.05). The best growth, feed efficiency and protein productive value were observed in the groups fed 5 or 20 g kg?1 glutamate supplementation at both lipid levels, with significant differences for LL diet (P < 0.05). A better feed efficiency was achieved with the HL diet (14.6–27.5% vs. 2.5–19.2% for LL diet). There were no significant differences in the proximate composition of carcass (animal excluding the digestive gland). However, a substantial amount of lipids accumulated in the digestive gland, mainly triglycerides, was detected as a consequence of higher lipid ingestion or glutamate supplementation. It was notable the lower percentages of phosphatidylethanolamine and phosphatidylcholine in the digestive gland of animals with best growth.  相似文献   
29.
The aim of the present work was to test the capacity of Octopus vulgaris to use carbohydrates supplied in three diets: a diet without added carbohydrates (diet C0: 500 g kg?1 water, 200 g kg?1 gelatine, 100 g kg?1 egg yolk powder, 50 g kg?1 freeze‐dried Sardinella aurita and 150 g kg?1 freeze‐dried Todarodes sagittatus) and two obtained by substituting 50 g kg?1 of T. sagittatus by glucose (diet GLU50) or by starch (diet STA50). The most stable and best‐accepted diet was STA50 (SFR 1.26%BW day?1) although there were no significant differences in the growth rates obtained with the three diets: 10.12 g day?1, 9.37 g day?1 and 11.22 g day?1 for C0, GLU50 and STA50, respectively (P > 0.05). The feed efficiency indices were better for GLU50, of particular note being the protein productive value of 71.88% and a feed conversion ratio lower than 1. Protein and lipid digestibility were similar in all the three diets (96–98% for proteins and 85–94% for lipids), whereas carbohydrate digestibility was higher in GLU50 (98%) than in C0 (84%) and STA50 (0.33%). The content of carbohydrates increased in muscle and the digestive gland as a consequence of the increased carbohydrates intake.  相似文献   
30.

Objectives

To compare propofol and alfaxalone, with or without midazolam, for induction of anesthesia in fentanyl-sedated dogs, and to assess recovery from total intravenous anesthesia (TIVA).

Study design

Prospective, incomplete, Latin-square study.

Animals

Ten dogs weighing 24.5 ± 3.1 kg (mean ± standard deviation).

Methods

Dogs were randomly assigned to four treatments: treatment P-M, propofol (1 mg kg?1) and midazolam (0.3 mg kg?1); treatment P-S, propofol and saline; treatment A-M, alfaxalone (0.5 mg kg?1) and midazolam; treatment A-S, alfaxalone and saline, administered intravenously (IV) 10 minutes after fentanyl (7 μg kg?1) IV. Additional propofol or alfaxalone were administered as necessary for endotracheal intubation. TIVA was maintained for 35–55 minutes by infusions of propofol or alfaxalone. Scores were assigned for quality of sedation, induction, extubation and recovery. The drug doses required for intubation and TIVA, times from sedation to end of TIVA, end anesthesia to extubation and to standing were recorded. Analysis included a general linear mixed model with post hoc analysis (p < 0.05).

Results

Significant differences were detected in the quality of induction, better in A-M than A-S and P-S, and in P-M than P-S; in total intubation dose, lower in P-M (1.5 mg kg?1) than P-S (2.1 mg kg?1), and A-M (0.62 mg kg?1) than A-S (0.98 mg kg?1); and lower TIVA rate in P-M (268 μg kg?1 minute?1) than P-S (310 μg kg?1 minute?1). TIVA rate was similar in A-M and A-S (83 and 87 μg kg?1 minute?1, respectively). Time to standing was longer after alfaxalone than propofol, but was not influenced by midazolam.

Conclusions and clinical relevance

Addition of midazolam reduced the induction doses of propofol and alfaxalone and improved the quality of induction in fentanyl-sedated dogs. The dose rate of propofol for TIVA was decreased.  相似文献   
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