Use of alkaline phosphatase staining to differentiate canine osteosarcoma from other vimentin-positive tumors     

Barger A  Graca R  Bailey K  Messick J  de Lorimier LP  Fan T  Hoffmann W 《Veterinary pathology》2005,42(2):161-165

Aspiration of lytic bone lesions is an excellent diagnostic test in the initial evaluation of primary bone neoplasia. However, cytologically, it can be difficult to differentiate osteosarcoma (OSA) from other bone neoplasms, including fibrosarcoma, chondrosarcoma, synovial cell sarcoma, and plasma cell myeloma. The purpose of this study is to determine the sensitivity and specificity of alkaline phosphatase (ALP) staining to differentiate OSA from other tumors that express vimentin by immunocytochemistry or immunohistochemistry. ALP is a hydrolytic enzyme present in multiple tissues including liver, kidney, intestine, placenta, and bone. Hypothetically, neoplasms actively producing bone should be specifically positive for ALP staining. Unstained, cytologic specimens were incubated for 8-10 minutes with nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate toluidine salt-phosphatase substrate. A positive reaction stains the membrane of the cells gray to black. Samples were counterstained with a Romanowsky's stain to determine whether the sample was of representative cellularity. A total of 61 vimentin-positive neoplasms have been evaluated and confirmed histopathologically. Tumors that expressed vimentin and were positive for ALP included 33 OSAs, one multi-lobular tumor of bone, one amelanotic melanoma, and one chondrosarcoma. Tumors that expressed vimentin and were negative for ALP included chondrosarcomas (three of four), multiple fibrosarcomas, and multiple synovial cell sarcomas. The sensitivity is 100%, and the specificity is 89%. In conclusion, ALP appears to be a highly sensitive and fairly specific marker in the diagnosis of OSA.  相似文献   

A critical role of interleukin-10 in the response of bovine macrophages to infection by Mycobacterium avium subsp paratuberculosis     

Weiss DJ  Evanson OA  de Souza C  Abrahamsen MS 《American journal of veterinary research》2005,66(4):721-726

OBJECTIVES: To evaluate the role of interleukin (IL)-10 in the inability of monocyte-derived bovine macrophages to kill Mycobacterium avium subsp paratuberculosis organisms in vitro. SAMPLE POPULATION: Monocytes were obtained from healthy adult Holstein dairy cows that had negative results when tested for infection with M avium subsp paratuberculosis. PROCEDURE: Monocyte-derived macrophages were incubated with M avium subsp paratuberculosis for 2, 6, 24, 72, or 96 hours with or without addition of saturating concentrations of a goat anti-human IL-10 that has been documented to neutralize bovine IL-10 activity. Variables assessed included ingestion and killing of M avium subsp paratuberculosis; expression of tumor necrosis factor (TNF)-alpha, IL-12, IL-8, major histocompatability (MHC) class II, vacuolar H+ ATPase, and B cell CLL/lymphoma 2 (BCL-2); production of nitric oxide; acidification of phagosomes; and apoptosis of macrophages. RESULTS: Neutralization of IL-10 enabled macrophages to kill 57% of M avium subsp paratuberculosis organisms within 96 hours. It also resulted in an increase in expression of TNF-alpha, IL-12, IL-8, MHC class II, and vacuolar H+ ATPase; decrease in expression of BCL-2; increase in acidification of phagosomes; apoptosis of macrophages; and production of nitric oxide. CONCLUSIONS AND CLINICAL RELEVANCE: The capacity of M avium subsp paratuberculosis to induce IL-10 expression may be a major determinant of virulence for this organism.  相似文献   

Experimental use of polyamide bands in combination with intramedullary pinning for repair of oblique femoral fractures in rabbits     

Carrillo JM  Sopena JJ  Rubio M  Redondo JI  Serra I  Soler C 《Veterinary surgery : VS》2005,34(4):387-392

OBJECTIVE: To evaluate the use of polyamide bands, manufactured for securing electrical cables, for repair of oblique femoral fractures in rabbits. STUDY DESIGN: Experimental study. ANIMALS: Twelve nulliparous, 21-25-week-old, California female rabbits. METHODS: Rabbits were divided into 3 groups (n=4) and studied for 14, 28, or 56 days. A Z-shaped mid-diaphyseal femoral osteotomy was repaired with a 2.5 mm intramedullary pin and a polyamide 6.6 cerclage band. Healing was evaluated at intervals by physical examination, limb use, femoral radiographs, and callus histology. RESULTS: Rabbits had early limb use with good wound healing. From the 1st day, movement of the hip and stifle joints was satisfactory. Radiographically, fractures were healed at 28 days. Histologically, there was no foreign body reaction and bone healing was normal. CONCLUSION: Nylon cerclage band application was accomplished easily, maintained reduction, and resulted in good healing and limb use. CLINICAL RELEVANCE: Sterilized nylon bands, manufactured for electrical use, can be used for cerclage in rabbits.  相似文献   

Tear urea nitrogen and creatinine levels in horse and their correlation with serum values     

Zapata GL  Britos RM  Pintos ME  Dreizzen E  Lausada NB  Arauz S 《Veterinary ophthalmology》2005,8(3):207-209

The objective of this paper was to determine the physiological values of urea nitrogen and creatinine in tears, and to compare the results with those obtained from serum. Thirty healthy thoroughbred horses were included in the study. Tear fluid samples were obtained using a glass capillary tube placed in lower conjunctival cul-de-sac. Blood samples were taken from the jugular vein. Tear and serum urea nitrogen and creatinine levels were quantitatively analyzed by an enzymatic colorimetric method. Urea nitrogen values were 4.22+/-1.84 mmol/l in tears and 4.44+/-1.78 mmol/l in serum, whereas creatinine values in tears were 14.14+/-7.74 micromol/l and in serum 147.63+/-12.17 micromol/l. Statistical analysis confirmed a significant correlation between serum and tear urea levels (P<0001). However, there was no significant correlation between blood and tear creatinine values. Mean value of creatinine obtained from tears was 9.6% of the mean value from serum. Urea nitrogen and creatinine levels can be measured in tears. A significant correlation was found between serum and tears urea levels. This finding may permit development of a new alternative laboratory diagnosis of uremia based on the content of urea in tears.  相似文献   

Feline infectious peritonitis (FIP) virus. III. Studies on the multiplication of FIP virus in the suckling mouse   总被引：1，自引：0，他引：1  

M C Horzinek  A D Osterhaus  R M Wirahadiredja  P de Kreek 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1978,25(10):806-815

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De invloed van het toevoegen van organisch materiaal aan de grond op het optreden vanRhizoctonia-ziekte en schurft bij aardrappelen     

S. de Boer 《European journal of plant pathology / European Foundation for Plant Pathology》1962,68(5):268-277

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NIGROPALLIDAL ENCEPHALOMALACIA IN HORSES IN NEW SOUTH WALES     

G. P. GARD B.V.Sc.  Ph.D.    W. G. de  SAREM M.V.Sc. P. J. AHRENS B.V.Sc. 《Australian veterinary journal》1973,49(2):107-108

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Hyoscyamus niger,a useful local lesion host for a mosaic virus in Hippeastrum     

G. T. N. de Leeuw 《European journal of plant pathology / European Foundation for Plant Pathology》1972,78(3):107-109

Samenvatting Bladeren vanHyoscyamus niger planten vertoonden chlorotische lokale lesies 7 dagen na inoculatie met sap van mozaïekziekeHippeastrum-planten. Met sap vanHippeastrum-planten zonder mozaïeksymptomen werd geen reactie verkregen. In dooppreparaten gemaakt van chlorotische lesies inHyoscyamus-bladeren en van bladeren vanHippeastrum planten met mozaïeksymptomen werden draadvormige virusdeeltjes van 648–772 nm aangetroffen. Het virus kon met sap geperst uit geïnoculeerdeHyoscyamus-planten weer op gezondeHippeastrum-planten worden overgebracht.  相似文献   

Bovine viral diarrhea-mucosal disease, abortion, and congenital cerebellar hypoplasia in a dairy herd   总被引：2，自引：0，他引：2  

R F Kahrs  F W Scott  A de Lahunta 《Journal of the American Veterinary Medical Association》1970,156(7):851-857

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Ten geleide     

J. Dekker  H. de Fluiter 《European journal of plant pathology / European Foundation for Plant Pathology》1969,75(1-2):7-7

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