Welfare and pregnancy rate of ewes undergoing transcervical artificial insemination with ketamine subarachnoid anesthesia     

Carneiro  Renata P. B.  Macedo  Gustavo G.  DeRossi  Rafael  Costa-e-Silva  Eliane V.  Souza  Maria Inês L. 《Tropical animal health and production》2019,51(5):1179-1186

Tropical Animal Health and Production - This study aimed to evaluate the impact of subarachnoid anesthesia with ketamine during transcervical artificial insemination (TCAI) on the welfare of ewes...  相似文献   

Evaluation of plasma angiopoietin‐2 and vascular endothelial growth factor in healthy dogs and dogs with systemic inflammatory response syndrome or sepsis     

Maya Knig  Alice Nentwig  Eliane Marti  Jelena Mirkovitch  Katja‐Nicole Adamik  Simone Schuller 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):569-577

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Selection of Leguminous Trees Associated with Symbiont Microorganisms for Phytoremediation of Petroleum-Contaminated Soil     

Ricardo Aparecido Bento  Orivaldo José Saggin-Júnior  Rosa Maria Pitard  Rosangela Straliotto  Eliane Maria Ribeiro da Silva  Sílvio Roberto de Lucena Tavares  Frederico Henrique Teixeira Gerken de Landa  Luiz Fernando Martins  Antonia Garcia Torres Volpon 《Water, air, and soil pollution》2012,223(9):5659-5671

Leguminous trees have a potential for phytoremediation of oil-contaminated areas for its symbiotic association with nitrogen-fixing bacteria and arbuscular mycorrhizal fungi (AMF). This study selects leguminous tree associated with symbiotic microorganisms that have the potential to remediate petroleum-contaminated soil. Seven species of trees were tested: Acacia angustissima, Acacia auriculiformis, Acacia holosericea, Acacia mangium, Mimosa artemisiana, Mimosa caesalpiniifolia, and Samanea saman. They were inoculated with AMF mix and nitrogen-fixing bacteria mix and cultivated over five oil levels in soils, with five replicates. The decreasing of total petroleum hydrocarbons (TPH) values occurred especially with S. saman and its symbiotic microorganisms on highest oil soil contamination. Despite the large growth of A. angustissima and M. caesalpiniifolia on the highest level of oil, these species and its inoculated microorganisms did not reduce the soil TPH. Both plants were hydrocarbon tolerant but not able to remediate the polluted soil. In contrast were significative hydrocarbon decrease with M. artemisiana under high oil concentrations, but plant growth was severely affected. Results suggest that the ability of the plants to decrease the soil concentration of TPH is not directly related to its growth and adaptation to conditions of contamination, but the success of the association between plants and its symbionts that seem to play a critical role on remediation efficiency.  相似文献   

Keratinocyte biology and pathology     

Eliane J. Müller  Arnaud Galichet  Dominique Wiener  Eliane Marti  Cord Drögemüller  Monika Welle  Petra Roosje  Tosso Leeb  Maja M. Suter 《Veterinary dermatology》2014,25(4):236-238

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Identification of an alpha-amylase inhibitor from Pterodon pubescens with ability to inhibit cowpea weevil digestive enzymes     

Silva DP  Casado-Filho EL  Corrêa AS  Farias LR  Bloch C  de Sa MF  Mendes PA  Quirino BF  Noronha EF  Franco OL 《Journal of agricultural and food chemistry》2007,55(11):4382-4387

Cowpea seeds (Vigna ungiculata) are widely cultivated by poor farmers in Latin America and Africa and are often severely damaged by the cowpea weevil Callosobruchus maculatus. A proteinaceous inhibitor of cowpea weevil digestive enzymes, PpAI, was purified from white sucupira seeds (Pterodon pubescens) and biochemically characterized in this study. Proteins were extracted from seeds and precipitated with ammonium sulfate at 100% saturation. This fraction was applied onto a Red-sepharose CL-6B column, and the retained peak showed 70% inhibitory activity toward larval C. maculatus digestive alpha-amylases. The retained peak was then purified using an analytical reversed-phase HPLC column. Purified PpAI showed 65% inhibitory activity against larval C. maculatus enzymes. Enzymatic assays also showed that the purified P. pubescens inhibitor was unable to reduce the activity of mammalian alpha-amylases, suggesting specificity toward insect enzymes. Moreover, artificial seeds containing PpAI were able to reduce larval weight by 36% and cause 55% mortality. Mass spectrometry and SDS-PAGE analyses indicated that PpAI showed a molecular mass of approximately 5.0 kDa. This alpha-amylase inhibitor, coming from a native Cerrado plant, could be used to construct a genetically engineered cowpea with enhanced resistance against weevil pests.  相似文献   

Behaviour of metolachlor and terbuthylazine in cultivated field lysimeters     

Liviana Leita  Paolo Ceccon  Cesare Marucchini  Claudio Mondini 《植物养料与土壤学杂志》1996,159(2):177-182

An experiment has been carried out in cultivated drainage lysimeters filled with reported soil in order to investigate on the disappearance and mobility of Metolachlor and Terbuthylazine as related to the nitrogen fertilization level. Two experimental units have been compared: in the O-N treatment the soil did not receive N fertilizers during the 7 years previous to the experiment, whereas in the H—N treatment the average application rate of nitrogen during the same period was 220 kg ha^?1 year^?1; a corn crop has been cultivated in the present experiment. The active ingredients have been analysed in the artificial soil profile (from 0 to 40 cm, by 10 cm soil layers) and in leachates. The disappearance rate of Terbuthylazine was affected by nitrogen fertilization level, as the half life resulted about 7 days higher in O—N (21.9 days) treatment than in H—N (14.6 days). On the contrary, N fertilization did not significantly modify the half life of Metolachlor (17.2 and 15.9 days at O—N and H—N, respectively). A N-dealkylated degradation product of Terbuthylazine has been found in the shallow soil layers. The distribution pattern of herbicides content in the soil profile showed that Terbuthylazine was less mobile than Metolachlor. Leachates did not contain Terbuthylazine at all, but amounts of Metolachlor close to the detection limits (25 μg L^?1).  相似文献   

Decomposition of lipids in soils: Free and esterified fatty acids,alcohols and ketones     

J. Moucawi  Eliane Fustec  P. Jambu  Rose Jacquesy 《Soil biology & biochemistry》1981,13(6):461-468

Because lipids are important to the properties of soils and the composition of sedimentary organic matter, the rates of decomposition of selected lipids in soils were determined. The rates of decomposition of total lipid extracts from plant materials and of carbohydrates showed marked differences that were dependent on the biological activity of the soils. In biologically-active soils, such as a rendzina or a brunic luvisol, pure lipids were readily degraded, regardless of their functional groups and chain length. An exception was montanic acid (nC-28), which was refractory in all soils studied. In acid soils, such as a glossic luvisol or a dystric histosol, both chain length and functional groups affected the rate of decomposition of lipids, e.g. after 4 weeks at 28 C, the ratio of decomposition of oleic acid to, stearic acid was as high as 5. The same effect was found when 3-octadecanone (C-18 ketone) was compared to myristone (C-27 ketone). In most soils, the rate of decomposition of free and esterified stearic acid was similar, implying preliminary enzymatic hydrolysis of both the waxes and the fats.  相似文献   

Immunopathogenesis and immunotherapy of Culicoides hypersensitivity in horses: an update     

Eliane Marti  Ella N. Novotny  Iva Cvitas  Anja Ziegler  A. Douglas Wilson  Sigurbjörg Torsteinsdottir  Antonia Fettelschoss-Gabriel  Sigridur Jonsdottir 《Veterinary dermatology》2021,32(6):579-e156

Culicoides hypersensitivity (CH) is a strongly pruritic dermatitis of horses, characterised histologically by mixed perivascular to diffuse cellular infiltrates of mononuclear cells and eosinophils, and caused by helper T cell (Th)2, immunoglobulin (Ig)E-mediated reactions to antigens present in the saliva of biting Culicoides midges. As treatment of CH is still not satisfactory, a better knowledge of the pathogenesis of CH is needed for the development of new therapeutic modalities. Accordingly, availability of pure Culicoides allergens instead of crude whole body extracts is essential for the improvement of in vitro diagnosis of CH and development of a more effective allergen immunotherapy (AIT). Beside the Th2 cytokines interleukin (IL)-4 and IL-13, recent studies have shown the involvement of thymic stromal lymphopoietin, IL-5 and IL-31 in the pathogenesis of CH. The importance of IL-5 and IL-31 has been confirmed through targeting these cytokines by active immunisation of CH patients, resulting in a significant decrease of CH lesion scores. Thirty Culicoides salivary allergens have been described over the last 10 years and produced as pure recombinant (r-) proteins. Use of a protein microarray has allowed the identification of the most relevant Culicoides r-allergens for CH in central and northern Europe. An immunisation protocol has been developed for preventive AIT against CH. Small amounts of r-Culicoides allergens injected into the submandibular lymph nodes with Alum/MPLA as adjuvants seem to induce a suitable immune response for both preventive and therapeutic AIT. The next years will reveal whether these recent advancements will translate into an improved diagnosis, treatment and prevention of CH.  相似文献   

Cardiorespiratory and blood gas alterations during laparoscopic surgery for intra-uterine artificial insemination in dogs     

Fukushima FB  Malm C  Andrade ME  Oliveira HP  Melo EG  Caldeira FM  Gheller VA  Palhares MS  Macedo SP  Figueiredo MS  Silva MX 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(1):77-79

Cardiorespiratory and blood gas alterations were evaluated in 6 healthy dogs that underwent a laparoscopic procedure using isoflurane anesthesia and carbon dioxide (CO(2)) pneumoperitoneum for 30 min. Heart rate, respiratory rate, body temperature, venous blood pH, partial pressure of CO(2) and oxygen, oxygen saturation, total carbon dioxide (TCO(2)) and bicarbonate were monitored. Significant alterations were hypercapnia, hypoventilation, and respiratory acidosis.  相似文献   

Microbiological Quality and Antibiotic Resistance Analysis of a Brazilian Water Supply Source     

Trajano Felipe Barrabas Xavier da Silva  D��bora Toledo Ramos  Maur��cio Dziedzic  C��ntia Mara Ribas de Oliveira  Eliane Carvalho de Vasconcelos 《Water, air, and soil pollution》2011,218(1-4):611-618

Water samples of the Passaúna River, Curitiba/Paraná (Brazil), were analyzed to determine total and thermotolerant coliform counts and Escherichia coli in order to provide information on human impacts on the water supply. Samples were collected and analyzed monthly, from March 2006 to February 2007, at five different locations along the river, and the multiple tube method was used to obtain total and thermotolerant coliform counts. The results varied from 130 MPN/100 mL to 1.6?×?10⁶ MPN/100 mL for total coliforms, while for thermotolerant coliforms the variation was between 40 MPN/100 mL and 5?×?10⁵ MPN/100 mL. The E. coli strains isolated from the samples were tested with 13 different antibiotics to determine their antibiotic resistance. The isolated strains were constantly sensitive to seven of the 13 antibiotics tested, and resistant to at least one of the other antibiotics. The results indicated that two factors could influence the increased contamination on this river, viz., seasonality parameters and domestic wastewater discharges. The determination of antibiotic resistance indices aimed to provide information on the anthropogenic influence. Only one of the locations investigated was considered critical due to the anthropogenic influence, with significant impacts from irregular domestic wastewater discharges.  相似文献