Comparison of cytopathic and noncytopathic isolates of bovine viral diarrhea virus by oligonucleotide fingerprinting.     

L L Bissey  A K Williams  S Bolin  E W Collisson 《Journal of veterinary diagnostic investigation》1991,3(1):16-21

The molecular technique of RNA fingerprinting was used to characterize the genomes of 5 isolates of bovine viral diarrhea virus (BVDV): 2 viral pairs from the same animal, BVD-ILN/BVD-ILC and BVD-TGAN/BVD-TGAC, and the cytopathic viral prototype, BVD-NADL. Oligonucleotide patterns from the viruses were compared, and unique and overlapping oligonucleotides were identified. A comparison of the fingerprints indicated that the genome of each virus was distinguishable by the T1 RNase oligonucleotide fingerprinting technique. The greatest similarity observed was between oligonucleotides from BVD-ILC and BVD-ILN. Eighteen large oligonucleotides were conserved in all 5 BVDV isolates studied. We found that within a pair of BVDV, the cytopathic fingerprint was different from the noncytopathic fingerprint, indicating that cytopathic and noncytopathic BVDV may be distinct viruses.  相似文献   

Procedure for processing of central nervous system tissue for immunofluorescence, light, and electron microscopic evaluation.     

R M Williams  S Krakowka  A Koestner 《American journal of veterinary research》1978,39(12):1946-1949

A rapid and simple procedure has been developed for the simultaneous localization of viral antigen in central nervous system tissues by immunofluorescnece and concomitant correlation with viral induced lesions in the contralateral brain hemisphere. In this study, one-half of the brain was excised from the dog under deep general anesthesia and samples were collected for immunofluorescence by snap freezing in liquid nitrogen. The other half of the brain was perfused under pressure with a buffered glutaraldehyde-paraformaldehyde mixture for subsequent light microscopic and ultrastructural evaluation. The technique was useful in studying central nervous system diseases in large outbred experimental subjects in which economic and other considerations developed for tissue processing in rodents was not feasible.  相似文献   

Studies on liquid hemicellulose and cane molasses as carbohydrate sources in urea-containing diets of sheep     

D L Williams  J D Moore  L C Martin  A D Tillman 《Journal of animal science》1969,28(5):667-672

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Limited survey of Georgia cattle for fat necrosis     

M M Forney  D J Williams  E M Papp  D E Tyler 《Journal of the American Veterinary Medical Association》1969,155(10):1603-1604

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Haematology of subclinical fatty liver in dairy cows     

I M Reid  A M Dew  L A Williams 《Research in veterinary science》1984,37(1):63-65

The relationship between liver fat content and haematology was investigated in 369 cows from eight herds sampled in the second week after calving. High levels of fat in the liver were associated with a depression in total white cell count and in neutrophils, eosinophils and lymphocytes. There was no correlation between liver fat content and the percentage of E rosetting lymphocytes, packed cell volume or haemoglobin concentration. The changes in peripheral white cell counts may be related to the increased incidence of post parturient disease in cows with fatty liver.  相似文献   

The control of Fannia canicularis (L.) in poultry houses using impregnated cords     

J R Williams 《British poultry science》1973,14(6):547-555

Comparative field trials of the use of ordinary and composite insecticide‐impregnated cords for the control of Fannia canicularis (L.) in poultry houses are described and shown to be capable of giving season‐long fly control. Dimethoate (30% w/v), fenchlorphos (12% w/v), trichlorphon (6.5% w/v) and, to a lesser extent, diazinon (20% w/v) are considered to be suitable insecticides for cord impregnation. Using impregnated cords at the rate of 0.98 m treated cord/m² of floor area, the estimated cost of fly control was £3.50–5.00 per 1000 birds per season depending on the management system employed in the poultry house.  相似文献   

A comparison of all-meat, semimoist, and dry-type dog foods as diets for growing beagles     

K M Goddard  G D Williams  P M Newberne  R B Wilson 《Journal of the American Veterinary Medical Association》1970,157(9):1233-1236

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Mold toxicity in swine and laboratory animals: effect of feeding corn inoculated with pure cultures of Fusarium roseum Ohio Isolate C     

D P Sharda  R F Wilson  L E Williams  L A Swiger  R F Cross 《Journal of animal science》1971,32(6):1169-1173

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Urine sulfated and nonsulfated bile acids as a diagnostic test for liver disease in cats     

Steiner JM  Williams DA  Twedt DC 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(5):605; author reply 605-605; author reply 606

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mRNA expression of components of the IGF system and of GH and insulin receptors in ovaries of broiler breeder hens fed ad libitum or restricted from 4 to 16 weeks of age     

Heck A  Metayer S  Onagbesan OM  Williams J 《Domestic animal endocrinology》2003,25(3):287-294

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