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81.
A rapid and simple procedure has been developed for the simultaneous localization of viral antigen in central nervous system tissues by immunofluorescnece and concomitant correlation with viral induced lesions in the contralateral brain hemisphere. In this study, one-half of the brain was excised from the dog under deep general anesthesia and samples were collected for immunofluorescence by snap freezing in liquid nitrogen. The other half of the brain was perfused under pressure with a buffered glutaraldehyde-paraformaldehyde mixture for subsequent light microscopic and ultrastructural evaluation. The technique was useful in studying central nervous system diseases in large outbred experimental subjects in which economic and other considerations developed for tissue processing in rodents was not feasible. 相似文献
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The relationship between liver fat content and haematology was investigated in 369 cows from eight herds sampled in the second week after calving. High levels of fat in the liver were associated with a depression in total white cell count and in neutrophils, eosinophils and lymphocytes. There was no correlation between liver fat content and the percentage of E rosetting lymphocytes, packed cell volume or haemoglobin concentration. The changes in peripheral white cell counts may be related to the increased incidence of post parturient disease in cows with fatty liver. 相似文献
85.
J R Williams 《British poultry science》1973,14(6):547-555
Comparative field trials of the use of ordinary and composite insecticide‐impregnated cords for the control of Fannia canicularis (L.) in poultry houses are described and shown to be capable of giving season‐long fly control. Dimethoate (30% w/v), fenchlorphos (12% w/v), trichlorphon (6.5% w/v) and, to a lesser extent, diazinon (20% w/v) are considered to be suitable insecticides for cord impregnation. Using impregnated cords at the rate of 0.98 m treated cord/m2 of floor area, the estimated cost of fly control was £3.50–5.00 per 1000 birds per season depending on the management system employed in the poultry house. 相似文献
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Steiner JM Williams DA Twedt DC 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(5):605; author reply 605-605; author reply 606
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Añor S Lipsitz D Williams DC Tripp L Willits N Maselli R LeCouteur RA 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(4):545-550
Single-fiber electromyography (SFEMG), a technique used to investigate neuromuscular transmission, has been described previously in the pelvic limb of dogs. Because preferential involvement of isolated muscle groups can occur in disorders of neuromuscular transmission, SFEMG was done in the peroneus longus (PL), extensor carpi radialis (ECR), and orbicularis oculi (OO) muscles of 10 adult, clinically normal dogs. Jitter was calculated as the mean absolute value of the consecutive differences in latency of 50 single muscle fiber action potentials after stimulation of intramuscular nerve bundles at the level of the motor point in at least 20 muscle fibers per muscle. Bilateral recordings were performed in 3 dogs. Mean jitter values were determined for each muscle, and differences among muscle groups and among dogs were compared. The upper limits of mean consecutive difference (mean plus 3 standard deviations) for the PL, ECR, and OO muscles were 21.94, 22.53, and 23.39 micros, respectively, and the upper limit of mean consecutive difference for individual muscle fibers in the respective fiber pools was 28.62, 36.39, and 35.68 micros. Jitter values for the ECR and OO were significantly higher than the jitter value for the PL muscle (P < .05). Significant differences among muscles or dogs or between sides were not observed for the ECR. Significant differences among dogs were observed for OO jitter values and were attributed to extremely low jitter values in 1 dog. Significant differences were demonstrated between sides for the PL and were attributed to small sample size. Results of this study provide normative data that can be used in the application of the stimulated SFEMG technique to dogs with suspected disorders of neuromuscular transmission. 相似文献