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61.
Nathalie C. Newby Cassandra B. Tucker David L. Pearl Stephen J. LeBlanc Ken E. Leslie Marina A.G. von Keyserlingk Todd F. Duffield 《The Canadian veterinary journal. La revue veterinaire canadienne》2014,55(5):442-448
Post-operative pain management following rumen surgery is not common practice. We examined the effect of providing the pain medication ketoprofen to dairy cattle following the first stage of a rumen cannulation surgery, which involves an incision in the body wall and exteriorizing and clamping the rumen. The results of this study provide clear evidence that the first stage of the surgery was painful and ketoprofen at the time of and 24 h following surgery, alleviated some, but not all, of the post-surgical pain. Pain mitigation should be included when performing flank surgery in cattle. 相似文献
62.
F. Widén H. Everett S. Blome J. Fernandez Pinero Å. Uttenthal M. Cortey T. von Rosen M. Tignon L. Liu 《Research in veterinary science》2014
Classical swine fever is one of the most important infectious diseases for the pig industry worldwide due to its economic impact. Vaccination is an effective means to control disease, however within the EU its regular use is banned owing to the inability to differentiate infected and vaccinated animals, the so called DIVA principle. This inability complicates monitoring of disease and stops international trade thereby limiting use of the vaccine in many regions. The C-strain vaccine is safe to use and gives good protection. It is licensed for emergency vaccination in the EU in event of an outbreak. Two genetic assays that can distinguish between wild type virus and C-strain vaccines have recently been developed. Here the results from a comparison of these two real-time RT-PCR assays in an interlaboratory exercise are presented. Both assays showed similar performance. 相似文献
63.
Erika Moutinho Costa Tatiana Xavier de CastroFernanda de Oliveira Bottino Rita de Cássia Nasser Cubel Garcia 《Veterinary microbiology》2014
To characterize canine coronavirus (CCoV) circulating in diarrheic puppies in Brazil, 250 fecal samples collected between 2006 and 2012 were tested. By using RT-PCR to partially amplify the M gene, CCoV RNA was detected in 30 samples. Sequence analysis of the M protein grouped eight strains with CCoV-I and another 19 with CCoV-II prototypes. To genotype/subtype the CCoV strains and assess the occurrence of single or multiple CCoV infections, RT-PCR of the S gene was performed, and 25/30 CCoV-positive strains amplified with one or two primer pairs. For 17/25 samples, single infections were detected as follows: six CCoV-I, nine CCoV-IIa and two CCoV-IIb. Eight samples were positive for more than one genotype/subtype as follows: seven CCoV-I/IIa and one CCoV-I/IIb. Sequence analysis revealed that the CCoV-I and IIa strains shared high genetic similarity to each other and to the prototypes. The Brazilian strains of CCoV-IIb displayed an aminoacid insertion that was also described in CCoV-IIb-UCD-1 and TGEV strains. Among the 25 CCoV-positive puppies, five had a fatal outcome, all but one of which were cases of mixed infection. The current study is the first reported molecular characterization of CCoV-I, IIa and IIb strains in Brazil. 相似文献
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Ulfert Focken Christian Schlechtriem Matthias von Wuthenau Armando García-Ortega Ana Puello-Cruz & Klaus Becker 《Aquaculture Research》2006,37(14):1429-1436
The free‐living soil nematode Panagrellus redivivus is well known to be an excellent food source for first feeding fish larvae. It represents an alternative to the highly expensive Artemia, which is commonly used. The lack of a proper method for mass production of P. redivivus has prevented its wider use in commercial hatcheries. A new cultivation method allows the production of a sufficient quantity of nematodes to deliver a standardized and permanently available live food of high quality, throughout the larval rearing period. In two experiments – carried out at the Centro de Investigación en Alimentación y Desarrollo, Mexico – several feeding regimes were established to prove the quality of the mass produced P. redivivus for larvae of Litopenaeus vannamei, the Pacific white shrimp. Two different nematode treatments were compared with a no‐feed group and a control group that was fed with Artemia. All treatments had an additional algal co‐feed and were run in five replicates. Panagrellus redivivus was cultured on two different media (wheat/corn flour and oat flour) to compare these for their suitability as high‐quality live food for the larvae. Shrimp fed nematodes grown on wheat/corn medium reached the postlarval stage earlier than those from other treatments. The nematode treatments showed promising results; however, further research is needed on the development of improved culture media or enrichment methods to further increase the nutritional value of P. redivivus. 相似文献
67.
von Gersdorff Jørgensen L Heinecke RD Skjødt K Rasmussen KJ Buchmann K 《Journal of fish diseases》2011,34(10):749-755
Freshwater fish are able to mount a protective immune response against the parasite Ichthyophthirius multifiliis (Ich) following a non‐lethal exposure. Factors involved in immunity comprise cellular and humoral factors, but antibodies have been suggested to play a prominent role in protection. However, host antibodies have not yet been demonstrated to bind to the parasite in situ. By the use of immunohistochemical techniques, this study demonstrated that IgT and IgM bind to surface structures, including cilia, on the early feeding stage of the parasite in the gills of immune rainbow trout, Oncorhynchus mykiss, shortly (2 h) after invasion. No binding of IgT and no or only a weak binding of IgM was observed on the parasites in the gills of similarly exposed but naïve rainbow trout. This study indicates that antibodies play an important part in the protection of immune fish against Ich although additional humoral and cellular factors may contribute to this reaction. 相似文献
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69.
Adrian Patrut Karl F. von Reden Robert Van Pelt Diana H. Mayne Daniel A. Lowy Dragos Margineanu 《Annals of Forest Science》2011,68(5):993-1003
• Introduction
For large trees without a continuous sequence of growth rings in their trunk, such as the African baobab (Adansonia digitata L.), the only accurate method for age determination is radiocarbon dating. As of today, this method was limited to dating samples collected from the remains of dead specimens. 相似文献70.
Estimating balanced structure areas in multi-species forests on the Sierra Madre Occidental,Mexico 总被引：1，自引：0，他引：1
Christian Wehenkel José Javier Corral-Rivas José Ciro Hernández-Díaz Klaus von Gadow 《Annals of Forest Science》2011,68(2):385-394