不同化学物质打破葡萄、桃休眠的研究   总被引：7，自引：1，他引：7  

方金豹  顾红  乔宝营  陈锦永  张威远 《果树学报》2005,22(5):470-473

以保护地栽培的葡萄、桃为试材,对氰氨类、细胞分裂素、赤霉酸和KNO3打破休眠的效果进行了研究。结果表明,1.5%单氰氨、11%石灰氮可明显促使葡萄提前萌芽,提早着色9-10d;50mg/LGA3和100mg/L6-BA对打破休眠基本无效。TDZ与单氰氨混用提高萌芽率的效果最好。在2个葡萄品种上,均没有观察到单氰氨或石灰氮处理对果穗或果实品质性状的不良影响。在华光油桃上,没有观察到化学物质对提早开花或成熟有明显的作用。对葡萄和桃上应用化学物质打破休眠的有关问题进行了讨论。  相似文献   

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro     

CAI Yun-feng  MIN Jun  FANG Tian-ling  CHU Zhong-hua  DENG Xiao-geng  HE Jing-song  CHEN Ji-sheng 《园艺学报》2003,19(8):1029-1033

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.  相似文献   

离体条件下水稻种衣剂对串珠镰刀菌生长及形态毒理影响     

刘西莉  张龙  李健强  房双龙 《植物病理学报》2002,32(4):332-337

 检测了黑龙江水稻主产区4个当地主栽品种的种子内部镰刀菌寄藏情况,测定了20%克福甲和20%克多甲种衣剂对种子带菌消毒处理效果及对水稻串珠镰刀菌(Fusarium moniliforme)的抑菌作用和联合毒力,并借助扫描电镜观察了上述2种混配种衣剂对串珠镰刀菌的形态毒理影响。结果表明,种子内部镰刀菌的分离频率高达56.7%~96.0%,其中串珠镰刀菌的分离频率为32.6%~48.2%,2种种衣剂对带菌种子具有显著的消毒处理效果。20%克福甲和20%克多甲种衣剂对镰刀菌F.moniliforme的毒力指数分别为457.11和802.04,增效倍数分别为6.53和0.13。20%克多甲种衣剂(多菌灵:甲基立枯磷为5:5,W/W)对串珠镰刀菌的抑菌作用优于20%克福甲种衣剂(福美双:甲基立枯磷为8:6,W/W),增效作用低于20%克福甲种衣剂。电镜观察表明,种衣剂低浓度至高浓度处理下均可引起串珠镰刀菌菌丝不同程度的异常生长,表现为主菌丝局部膨大或形成菌丝束,菌丝顶端异常膨大、缢缩或形成穗状和花絮状分枝。  相似文献   

施氮量对棉田主要害虫种群动态的影响   总被引：5，自引：0，他引：5  

崔金杰  夏敬源  王春义  马丽华  雒君瑜 《中国植保导刊》2003,23(1):3-5

通过不同施氮肥量对棉田主要害虫种群动态影响的研究结果表明,在低、中、高3种施氮肥水平处理条件下,随着氮肥量的增加,棉田棉铃虫落卵量增加,棉苗蚜、棉叶螨种群数量减少,发生为害减轻,但棉伏蚜种群数量增加。中等施氮肥条件下,第二、三代棉铃虫幼虫数量高于低和高施氮肥处理,对棉铃虫幼虫的生长发育和存活较为有利。  相似文献   

引起糖甜菜细菌性叶斑病的萎蔫短小杆菌新致病变种   总被引：3，自引：0，他引：3  

陈永芳  郭坚华  方中达 《植物病理学报》2003,33(5):396-400

 1995年在内蒙古临河市新发现了糖甜菜细菌性叶斑病,从病斑所分离的10个细菌菌株经柯赫氏法则验证,均确系该病的病原菌。采用形态观察、表型特征和生理生化特性测定、数值分析、血清学反应、细胞化学成分分析、DNA G+C mol%和DNA-DNA同源性测定进行了鉴定,并与植物病原棒形细菌15个标准菌株进行了比较。该病原菌为革兰氏阳性细菌,不规则短杆状,有一根鞭毛、亚极生或侧生,结合其生理生化特性、细胞化学成分和DNA G+C mol%和DNA-DNA同源性测定结果,认为应属于短小杆菌属(Curtobacterium)的萎蔫短小杆菌(Cur. flaccumfaciens),数值分析也支持这一结论。此外,据血清学反应结果及其对短小杆菌属的其它植物寄主的致病情况,认为该病原菌应是萎蔫短小杆菌种下的一个新的致病变种,定名为Curtobacterium flaccumfaciens pv. beticola pv. nov. Chen et al.,2000(萎蔫短小杆菌糖甜菜致病变种)。  相似文献   

大米粉的猪回肠氨基酸真消化率测定     

范国英  王永军钟华 王秦晋 《家畜生态》2004,25(2):18-21

大米加工副产品大米粉长期以来被添加到米糠中利用，显著降低了其利用率。本试验用三头施以回直肠吻合术猪，采用单因子设计，以大米粉为半纯合日粮，无氮日粮由醋酸纤维素提供粗纤维来测定内源值，测定了大米粉的猪回肠末端氨基酸表观消化率和真消化率。大米粉的回肠末端氨基酸的表观消化率的平均值为76．20％，真消化率的平均值为85.86％。这些数据可为我国氨基酸消化率数据库提供参考，并且可作为以可消化氨基酸为基础配制日粮时的依据。  相似文献   

关于修订桃种质资源(Prunus persica)描述体系的建议   总被引：2，自引：2，他引：2  

王力荣  朱更瑞  方伟超 《果树学报》2004,21(6):582-585

评价是种质资源工作的重要环节之一,是资源利用与共享的基础。目前,中国桃种质资源的评价描述体系一般是按照中国1990年编制的“桃种质描述符”进行。通过对国际植物遗传资源委员会1980年编制“桃描述系统″和中国“桃种质描述符”在评价的层次、评价内容、评价方法和评价标准的系统比较,以及2个标准在种质资源科研实践使用过程中存在的不足之处,结合中国桃种质资源工作的特点和国外资源的描述方法的新进展,提出中国桃种质资源描述系统的修改建议,为建立中国标准化、数字化的桃种质资源描述系统奠定基础。  相似文献   

Upregulation of neurokinin A levels by nerve growth factor in the experimental asthmatic guinea pig     

FANG Xiu-bin  LIU Xiao-xiang  ZHANG Hong 《园艺学报》2004,20(1):80-82

AIM: To explore the regulatory mechanism of nerve growth factor (NGF) on neurokinin A in the experimental asthmatic guinea pig. METHODS: Radioimmunoassay was used to determine the alteration of neurokinin A levels in the lower respiratory tract and visceral sensory afferent sites while NGF was absent (inhalation of NGF antibody through nasal cavity) in the asthmatic guinea pig. RESULTS: The contents of neurokinin A in the trachea, bronchus, lung, C₇-T₅ spinal ganglia and the correspondent spinal dorsal horn, nodose ganglia and solitary nucleus area in the experimental asthmatic guinea pig with the absent of NGF in the respiratory tract were much lower than those in the asthmatic and control groups (P＜0.01). CONCLUSION: NGF upregulated the contents of neurokinin A in the lower respiratory tract and visceral sensory sites of the experimental asthmatic guinea pig, and both might be involved in the pathogenesis of asthma.  相似文献   

Ginkgo biloba extract enhances c-jun expression and attenuates motoneuron death induced by root avulsion     

LI Fang-lan  ZHOU Li-hua  YUAN Qun-fang  YAO Zhi-bin  WU Wu-tian 《园艺学报》2004,20(10):1783-1785

AIM: To investigate the influence of Ginkgo biloba extract (EGb761) on c-jun expressions and motoneurons survival following root avulsion. METHODS: One hundred and eighty adult Sprague-Dawley female rats were randomly divided into control and EGb761 groups. Immediately after avulsion of C5-T1 nerve roots, the rats were injected ip with either 1 mL of EGb761 25 mg·kg^-1·d^-1 or the same volume of normal saline, and the treatment repeated everyday. At 4 h to 6 weeks following avulsion, the C7 spinal segments of all rats were collected and prepared for c-jun immunocytochemistry and neutral red stain. The numbers of c-jun positive and survival motoneurons were counted and compared between two groups at each time point. RESULTS: In control rats following avulsion, c-jun positive motoneurons appeared at 4 h, reached its maximum at 1 d and declined to 2 weeks. Avulsion-induced motoneurons death started at 2 weeks, climbed to its maximum at 4 weeks-6 weeks. In EGb761 treated rats, both numbers of c-jun positive and survival motoneurons were more than that in control group at each time point. CONCLUSION: EGb761 attenuates avulsion-induced motoneurons death, and this effect may be related to up-regulation of c-jun gene in avulsed motoneurons.  相似文献   

Expression of vascular endothelial growth factor in chronic inflammatory mucosa of lacrimal sac     

ZHANG Tao  WANG Ji-qun  WANG Li-hua  SHAN Yan-chun 《园艺学报》2004,20(3):459-461

AIM: To study the expression of vascular endothelial growth factor (VEGF) in inflammatory mucosa of lacrimal sac. METHODS: Immunohistochemical S-P method was used to examine the expression of VEGF in the mocusa from 12 patients with chronic dacryocystitis and 8 volunteers. RESULTS: The positive rates of VEGF expression in different parts of the mocusa were: basal lamina: 44.3%±7.6％; surface epithelium: 16.9%±4.6％; connective tissue: 15.2%±4.9％, all normal mocusa of 8 cases were negative. There was a significant difference between the two groups (P<0.01), a significant difference among each part of the chronic inflammatory mocusa of lacrimal sac. CONCLUSION: VEGF may play an important role in hyperplasia of inflammatory mucosa of lacrimal sac.  相似文献