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41.
Shiga toxin-producing Escherichia coli (STEC) strains are responsible for outbreaks of human intestinal diseases worldwide. Pigeons are distributed in public areas
and are potential reservoirs for pathogenic bacteria. One hundred fifty-four fresh fecal samples were obtained from trapped
pigeons in southeast of Iran and were cultured for isolation of E. coli. The isolates were examined to determine the prevalence of stx1, stx2, and eae genes, antimicrobial resistance, and their phylotypes. The confirmed E. coli isolates (138) belong to four phylogenetic groups: A (54.34%), B1 (34.05%), B2 (3.62%), and D (7.79%). Thirteen (9.42%) isolates
were positive for one of the examined genes. Eight isolates (5.79%) were positive for eae, four (2.89%) for stx2, and one isolate (1.44%) for stx1 gene. Phylotyping assays showed that eight eae-positive isolates fall into three phylogroups; A (three isolates), B1 (three isolates), and D (two isolates), whereas four
stx2-positive isolates belonged to the A (three isolates) and D (one isolate) groups. The stx1-positive isolate belonged to phylogroup A. One hundred six isolates (76.81%) showed resistance to at least one of the selected
antibacterial agents. The maximum resistance rate was against oxytetracycline (73.91%), and the minimum was against flumequine
(2.17%). Twenty different patterns of drug resistance were observed. According to the results, pigeons could be considered
as carriers of STEC strains. However, E. coli isolates of pigeon feces increase the potential of these birds to act as a reservoir of multiple antibiotic resistant bacteria. 相似文献
42.
Amine Moubarik Bertrand Charrier Fatima Charrier Antonio Pizzi Ahmed Allal 《Annals of Forest Science》2009,66(1):109-109
43.
Noreen Fatima Muhammad Shahid Ghulam Abbas Mubshar Hussain Muhammad Nafees 《Journal of plant nutrition》2018,41(12):1555-1565
Salinity is the major environmental stress that affects the growth and productivity of plants. The present study was conducted to determine the effect of salinity on growth and ions uptake by moringa (Moringa oleifera L.) plant. The experiment was carried out in two phases. Initially, a germination test was conducted in the laboratory under the different salinity levels (control, 5, 10, 15, and 20 dS m?1) and found that moringa seeds were germinated only at 5 and 10 dS m?1 salinity levels, and no germination occurred at higher salinity levels (15 and 20 dS m?1). The experiment was laid out in a completely randomized design (CRD) with five replications. In the second phase, three-week old nursery grown plants of moringa were shifted in pots under the five salinity levels (control, 5, 10, 15, and 20 dS m?1). The experiment was laid out in CRD and replicated four times. In pot experiment, the root, shoot length, and dry weights were significantly affected by increasing the salinity levels. The uptake of K+ and Ca2+ was highly affected at different salinity levels as compared to control and Na+ ions accumulation was higher in roots rather than shoot. The results reveal that moringa plant can germinate, survive, and can be cultivated in areas with moderate saline condition. 相似文献
44.
Antibody Prevalence and Risk Factors for Toxoplasma gondii Infection in Women from Multan,Pakistan
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M. M. Nazir M. Akhtar A. Maqbool A. Waheed M. A. Sajid M. A. Ali M. Oneeb M. A. Alam A. N. Ahmad N. Nazir S. Fatima D. S. Lindsay 《Zoonoses and public health》2017,64(7):537-542
Toxoplasma gondii infections are prevalent in humans and warm‐blooded animals. Maternal infections during pregnancy may have devastating consequences for transplacentally infected neonates. This study was conducted to examine the seroprevalence of antibodies to T. gondii in pregnant women of childbearing age and determine risk factors associated with pregnancy history, pet ownership, social and cultural factors at Nishtar Hospital, Multan. Samples were collected from 403 women and examined using a commercially available enzyme‐linked immunosorbent assay (ELISA). The overall prevalence of antibodies to T. gondii was 17.6% (71) in the 403 samples collected from women. Antibodies to T. gondii were present in 19.4% (45) of 232 pregnant women and 15.2% (26) of the samples from 171 non‐pregnant women. This study identified miscarriage history, pet ownership, type of residence, marital status, source of drinking water and eating habits as significant (P < 0.05) risk factors associated with the presence of antibodies to T. gondii infection. Seroprevalence was not significantly different (P > 0.05) in women from different ethnic groups based upon lifestyle and culture. 相似文献
45.
Noshin Ilyas Fakhra Ambreen Nazima Batool Muhammad Arshad Roomina Mazhar Fatima Bibi 《Communications in Soil Science and Plant Analysis》2018,49(2):148-158
Crop rotation is a supportive management practice in which legumes greatly improve the growth and yield of subsequent cereal crops, particularly wheat. In this study, effects of mung bean (Vigna radiata)?? on the yield and quality of grains of following wheat crop were determined. An experiment was carried out with two sets of wheat (Triticum aestivum) crops: one grown following mung bean (Vigna radiata) crop and the other grown after a fallow period. Concentrations of macronutrients, sugar, protein, amino acids, and phytohormones of wheat were determined. The grain protein concentration of wheat was improved if cultivated after mung bean, and nitrogen and other macronutrients of wheat were increased significantly. In case of phytohormone contents in the wheat crop, abscisic acid concentration showed no change, but the concentrations of gibberellin increased significantly by 41% and indole acetic acid by 30% as a result of crop rotation. Sugar content in wheat cultivated after mung bean BRM-318 showed 10% increase and wheat protein content increased by 17% and 20%, respectively. The present study demonstrated that crop rotation simulated yield and improved the nutritional value of wheat grain compared to wheat which followed the fallow period. 相似文献
46.
This study was carried out to determine the seroprevalence of feline leukemia virus (FeLV), feline immunodeficiency virus (FIV) and Toxoplasma gondii (T. gondii) infection among stray and owned cats in southeastern Iran and to identify the influence of age, sex, lifestyle, health status, and laboratory findings on seropositivity. The overall infection rate for FIV, FeLV, and T. gondii was 19.2%, 14.2%, and 32.1% respectively. Results of the multivariate logistic regression analysis showed that old adults more likely to be seropositive than juveniles for FIV, FeLV, and T. gondii (adjusted odds ratios [ORs], 1.84, 1.56, and 2.57 respectively). Anemic and diseased cats ([ORs], 6.62 and 0.9) were at a greater risk of testing positive for FeLV. Male cats were 4.91 times as likely to have FIV as were female and hyperglobulinemia was significantly more prevalent in FIV-infected cats ([ORs], 3.4). In conclusion, FIV and FeLV seem to be endemic in Iran and retroviral-associated immunosuppression may be a risk factor for active toxoplasmosis in infected cats. 相似文献
47.
Shirin Karimi Abdolreza Mohamadnia Seyed Alireza Nadji Reza Yadegarazari Adnan Khosravi Naghmeh Bahrami Massoud Saidijam 《Iranian Biomedical Journal》2015,19(1):17-22
Introduction: Although extensive research has been conducted on lung cancer markers, a singular clinically applicable marker has not been found yet. The objective of this study was to evaluate the sensitivity and the specificity of carcinoembryonic antigen (CEA) mRNA and lung-specific X protein (LUNX) mRNA biomarkers in peripheral blood to detect lung cancer individually and simultaneously. Methods: Thirty patients affected by lung cancer and 30 healthy individuals were studied in this research. Three vials of cDNA were made from each sample after taking peripheral blood samples and extracting total RNA. Each sample was examined by the real-time RT-PCR technique. The result from each vial was then compared with the sensitivity of overall marker. Results: The CEA mRNA was positive in 24 out of 30 lung cancer patients. Hence, its sensitivity was determined at 80%, differing significantly from that observed in healthy individuals, where 11 positive cases were seen. The overall sensitivity of this marker was significantly associated with positivity in vials 2 and 3 but not in vial 1. The LUNX mRNA was positive in 21 out of 30 patients, indicating 70% sensitivity. This finding significantly differed from that in healthy individuals. The overall sensitivity of this marker was significantly associated with positivity in vials 1 and 3, but not in vial 2. In 93.3% of the patients, at least one positive marker was observed. Conclusion: The mentioned mRNA could be suggested as sensitive and specific markers in peripheral blood for primary diagnosis of lung cancer.Key Words: Lung neoplasms, RNA, Carcinoembryonic antigen, Sensitivity and specificity 相似文献
48.
Erika Valéria Saliba Albuquerque Poliene Martins Costa Ana Cristina Meneses Mendes Gomes Marcilene Santos Antonio Alves Pereira Michel Nicole Diana Fernandez Maria Fatima Grossi-de-Sa 《European journal of plant pathology / European Foundation for Plant Pathology》2010,127(3):365-373
Root-knot nematodes (RKN) are obligate parasite species of the genus Meloidogyne that cause great losses in Arabica coffee (Coffea arabica L.) plantations. Identification of resistant genotypes would facilitate the improvement of coffee varieties aiming at an
environmental friendly and costless nematode control. In this work, the C. arabica genotype ‘UFV 408-28’ was found to be resistant to the most destructive RKN species M. incognita. Pathogenicity assays indicated that the highly aggressive populations of M. incognita races 1, 2 and 3 were not able to successfully reproduce on ‘UFV 408-28’ roots and displayed a low gall index (GI = 2). An
average reduction of 87% reduction of the M. incognita population was observed on ‘UFV 408-28’ when compared to the susceptible cultivar ‘IAC 15’. By contrast, ‘UFV 408-28’ was
susceptible to the related species M. exigua and M. paranaensis (GI = 5 and 4, respectively). Histological observations performed on sections of UFV408-28 roots infected with M. incognita race 1 showed that nematode infection could be blocked right after penetration or during migration and establishment stages,
at 6 days, 7 days and 8 days after infection (DAI). Fluorescence and bright field microscopy observations showed that root
cells surrounding the nematodes exhibited HR-like features such as accumulation of phenolic compounds and a necrotic cell
aspect. In the susceptible ‘IAC 15’ roots, 6 DAI, feeding sites contained giant cells with a dense cytoplasm. Necrotic cells
were never observed throughout the entire infection cycle. The HR-like phenotype observed in the ‘UFV 408-28’—M. incognita interaction suggests that the coffee resistance may be mediated by a R-gene based immunity system and may therefore provide new insights for understanding the molecular basis of RKN resistance
in perennial crops. 相似文献
49.
Wassila Tighzert Abderrahmane Habi Abdellah Ajji Tahar Sadoun Fatima Boukraa-Oulad Daoud 《Fibers and Polymers》2017,18(3):514-524
In this work, electrospinning of poly(lactic acid) (PLA), chitosan and their blends has been investigated, and nanofibers with a diameter ranging from 90 nm to 1.9 microns were produced and used as carriers for immobilization of the phospholipase A1. A strong influence of chitosan (CS) and the solvent trifluoroacetic acid (TFA) on the morphology, distribution of the nanofibers diameter and on their hydrophobicity was observed. The yield of phospholipase A1 (PLA1) on non-woven fibers was evaluated using the method of Bradford. Their activities and their reutilisability were assessed titrimetrically using soybean lecithin as substrate. The results showed that the degree of immobilization on the non-woven fibers of pure PLA and mixtures PLA/CS4 and PLA/SC6 are 73, 54, 45 % respectively and can be reused up to 4 cycles without significant loss of enzyme activity. Moreover, a remarkable improvement of the activity of phospholipase A1 on non-woven based on pure PLA fibers was observed, indicating that most of the enzymes were probably in their active form. 相似文献
50.
Márcia Rufini Michele Aparecida Pereira da Silva Paulo Ademar Avelar Ferreira Alice de Souza Cassetari Bruno Lima Soares Messias José Bastos de Andrade Fatima Maria de Souza Moreira 《Biology and Fertility of Soils》2014,50(1):115-122
Cowpea (Vigna unguiculata (L.) Walp.) is one of the most important crops in the north and northeast regions of Brazil, where it is grown under the subsistence regime, mainly because of its adaptation to the local edaphoclimatic conditions. This species is an important legume in Brazil because of its significant nutritional value in the diet of low-income populations. Moreover, it is able to benefit from the process of biological nitrogen fixation, which leads to a significant reduction in production costs. The objective of this research was to evaluate the agronomic efficiency of bacterial strains approved by the Ministry of Agriculture, Livestock and Supply for commercial inoculant production when in symbiosis with cowpea in a Rhodic Eutrudox in Lavras, MG, as well as to identify and verify the efficiency of the native populations that nodulate cowpea. Agronomic efficiency of nitrogen-fixing nodulating bacterial strains (UFLA 03-84, INPA 03-11B, and BR 3267) in symbiosis with cowpea was evaluated in the field. Subsequently, the efficiency and diversity of isolates from the nodules obtained in the field were evaluated under greenhouse conditions. The genetic diversity of the efficient isolates was determined by partial sequencing of the 16S rRNA gene. Grain yields obtained by the inoculation of seeds in the field with the strains were similar to those of the controls without inoculation with and without mineral N addition indicating the existence of efficient native populations. In the native community, we identified isolates with similar or higher efficiency than the strains approved for cowpea inoculation and the control that received mineral N. The isolates subjected to partial sequencing of the 16S rRNA gene were phylogenetically related to the Bradyrhizobium genus. 相似文献