Quince (Cydonia oblonga miller) fruit characterization using principal component analysis     

Silva BM  Andrade PB  Martins RC  Valentão P  Ferreres F  Seabra RM  Ferreira MA 《Journal of agricultural and food chemistry》2005,53(1):111-122

This paper presents a large amount of data on the composition of quince fruit with regard to phenolic compounds, organic acids, and free amino acids. Subsequently, principal component analysis (PCA) is carried out to characterize this fruit. The main purposes of this study were (i) the clarification of the interactions among three factors-quince fruit part, geographical origin of the fruits, and harvesting year-and the phenolic, organic acid, and free amino acid profiles; (ii) the classification of the possible differences; and (iii) the possible correlation among the contents of phenolics, organic acids, and free amino acids in quince fruit. With these aims, quince pulp and peel from nine geographical origins of Portugal, harvested in three consecutive years, for a total of 48 samples, were studied. PCA was performed to assess the relationship among the different components of quince fruit phenolics, organic acids, and free amino acids. Phenolics determination was the most interesting. The difference between pulp and peel phenolic profiles was more apparent during PCA. Two PCs accounted for 81.29% of the total variability, PC1 (74.14%) and PC2 (7.15%). PC1 described the difference between the contents of caffeoylquinic acids (3-O-, 4-O-, and 5-O-caffeoylquinic acids and 3,5-O-dicaffeoylquinic acid) and flavonoids (quercetin 3-galactoside, rutin, kaempferol glycoside, kaempferol 3-glucoside, kaempferol 3-rutinoside, quercetin glycosides acylated with p-coumaric acid, and kaempferol glycosides acylated with p-coumaric acid). PC2 related the content of 4-O-caffeoylquinic acid with the contents of 5-O-caffeoylquinic and 3,5-O-dicaffeoylquinic acids. PCA of phenolic compounds enables a clear distinction between the two parts of the fruit. The data presented herein may serve as a database for the detection of adulteration in quince derivatives.  相似文献   

Phenolic compounds in external leaves of tronchuda cabbage (Brassica oleracea L. var. costata DC)     

Ferreres F  Valentão P  Llorach R  Pinheiro C  Cardoso L  Pereira JA  Sousa C  Seabra RM  Andrade PB 《Journal of agricultural and food chemistry》2005,53(8):2901-2907

Glycosylated kaempferol derivatives from the external leaves of tronchuda cabbage (Brassica oleracea L. var. costata DC) characterized by reversed-phase HPLC-DAD-MS/MS-ESI were kaempferol 3-O-sophorotrioside-7-O-glucoside, kaempferol 3-O- (methoxycaffeoyl/caffeoyl)sophoroside-7-O-glucoside, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3-O-sophorotrioside-7-O-sophoroside, kaempferol 3-O-sophoroside-7-O-sophoroside, kaempferol 3-O-tetraglucoside-7-O-sophoroside, kaempferol 3-O-(sinapoyl/caffeoyl)sophoroside-7-O-glucoside, kaempferol 3-O-(feruloyl/caffeoyl)sophoroside-7-O-glucoside, kaempferol 3-O-sophorotrioside, kaempferol 3-O-(sinapoyl)sophoroside, kaempferol 3-O-(feruloyl)sophorotrioside, kaempferol 3-O-(feruloyl)sophoroside, kaempferol 3-O-sophoroside, and kaempferol 3-O-glucoside. These acylated derivatives are reported for the first time in nature, with the exception of kaempferol 3-O-(sinapoyl)sophoroside. Quantification of the identified compounds was achieved by HPLC-DAD and carried out in samples cultivated under conventional or organic practices and collected at different times. In general, samples from organic production exhibited higher total phenolics content than those from conventional practices collected in the same period.  相似文献   

Influence of two fertilization regimens on the amounts of organic acids and phenolic compounds of tronchuda cabbage (Brassica oleracea L. Var. costata DC)     

Sousa C  Valentão P  Rangel J  Lopes G  Pereira JA  Ferreres F  Seabra RM  Andrade PB 《Journal of agricultural and food chemistry》2005,53(23):9128-9132

A phytochemical study was undertaken on tronchuda cabbage (Brassica oleracea L. var. costata DC) cultivated under conventional and organic practices and collected at different times. Six organic acids (aconitic, citric, ascorbic, malic, shikimic, and fumaric acids) were identified and quantified by HPLC-UV. Qualitative and quantitative differences were noted between internal and external leaves. Analysis of the phenolics of the internal leaves was achieved by HPLC-DAD, and the phenolic profile obtained was revealed to be distinct from that of the external leaves. By this means were identified and quantified 11 compounds: 3-p-coumaroylquinic acid, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3-O-(caffeoyl)sophoroside-7-O-glucoside, kaempferol 3-O-(sinapoyl)sophoroside-7-O-glucoside, kaempferol 3-O-(feruloyl)sophoroside-7-O-glucoside, kaempferol 3-O-sophoroside, two isomeric forms of 1,2-disinapoylgentiobiose, 1-sinapoyl-2-feruloylgentiobiose, 1,2,2'-trisinapoylgentiobiose, and 1,2'-disinapoyl-2-feruloylgentiobiose. In general, internal leaves exhibited more constant chemical profiles.  相似文献   

Analysis of the discriminating power of agro/morphological and biochemical descriptors in a sample of the Spanish collection of barley (Hordeum vulgare L.)     

Magdalena Ruiz  Federico Varela  Jose M. Carrillo 《Genetic Resources and Crop Evolution》1997,44(3):247-255

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Population structure of Phytophthora infestans collected on potato and tomato in Italy     

Amanda C. Saville  Federico La Spada  Roberto Faedda  Quirico Migheli  Bruno Scanu  Paolo Ermacora  Giovanna Gilardi  Giorgia Fedele  Vitorrio Rossi  Nicolo Lenzi  Antonino Testa  Mohamed Bechir Allagui  Marwa Moumni  Enza Dongiovanni  Fatma Zohra Rekad  David E. L. Cooke  Antonella Pane  Santa O. Cacciola  Jean B. Ristaino 《Plant pathology》2021,70(9):2165-2178

Late blight caused by the oomycete Phytophthora infestans is a disease of potato and tomato of worldwide relevance and is widespread throughout Europe and the Mediterranean region. While pathogen populations in northern Europe have been sampled and characterized for many years, the genetic structure of populations from southern Europe, including Italy, has been less studied. Between 2018 and 2019, we collected 91 samples of P. infestans from potato and tomato crops in Italy, Algeria, and Tunisia on FTA cards and genotyped them using 12-plex microsatellites. These samples were compared to genotypes of P. infestans previously collected within the framework of the EuroBlight network and from published sources. Four clonal lineages were identified: 13_A2 (Blue 13), 2_A1, 23_A1, and 36_A2. Two other isolates collected could not be matched to any currently known clonal lineage. The 13_A2 and 36_A2 lineages were found exclusively in southern Italy and Algeria, while 2_A1 was only found in Algeria. This is the first report of the 36_A2 lineage in Italy. Two isolates from Solanum nigrum were 13_A2, suggesting this weed host could be a reservoir of inoculum. The 23_A1 lineage was found widely on infected tomato crops in Italy and is the same as the lineage US-23 that is widespread in North America. Differences in genotypes across the country suggests that there may be different sources of introduction into Italy, possibly via infected seed tubers from other countries in Europe, tubers for consumption from North Africa, or tomatoes.  相似文献   

Assessing temporal variation of primary and ecosystem production in two Mediterranean forests using a modified 3-PG model     

Angelo Nolè  Alessio Collalti  Federico Magnani  Pierpaolo Duce  Agostino Ferrara  Giuseppe Mancino  Serena Marras  Costantino Sirca  Donatella Spano  Marco Borghetti 《Annals of Forest Science》2013,70(7):729-741

Context

Forest ecosystem carbon uptake is heavily affected by increasing drought in the Mediterranean region.

Aims

The objectives of this study were to assess the capacity of a modified 3-PG model to capture temporal variation in gross primary productivity (GPP), and ecosystem net carbon uptake (NEE) in two Mediterranean forest types.

Methods

The model was upgraded from a monthly (3-PG) to a daily time step (3-PG_day), and a soil water balance routine was included to better represent soil water availability. The model was evaluated against seasonal GPP and NEE dynamics from eddy covariance measurements.

Results

Simulated and measured soil water content values were congruent throughout the study period for both forest types. 3-PG_day effectively described the following: GPP and NEE seasonal patterns; the transition of forest ecosystems from carbon sink to carbon source; however, the model overestimated diurnal ecosystem respiration values and failed to predict ecosystem respiration peaks.

Conclusions

The model served as a rather effective tool to represent seasonal variation in gross primary productivity, and ecosystem net carbon uptake under Mediterranean drought-prone conditions. However, its semi-empirical nature and the simplicity inherent in the original model formulation are obstacles preventing the model working well for short-term daily predictions.  相似文献   

Effects of thinning and canopy type on growth dynamics of Pinus sylvestris: inter-annual variations and intra-annual interactions with microclimate     

Irantzu Primicia  J. Julio Camarero  J. Bosco Imbert  Federico J. Castillo 《European Journal of Forest Research》2013,132(1):121-135

We assessed the effects of thinning (0, 20 and 30 % extraction of basal area) and canopy type (pine–beech vs. pine plots, beech accounting for 12 % of total basal area) on radial growth of dominant and codominant Scots pine at inter-annual scale and on microclimatic conditions, radial growth and xylogenesis 9 years after thinning at intra-annual scale. Thinning weakly affected pine growth, which was enhanced 3 years after harvesting. Over time, a gradual reduction in pine growth in mixed canopy relative to pure canopy occurred only in unthinned plots apparently due to beech expansion. Indeed, 9 years after thinning, a higher seasonal radial increment and a greater number of tracheids were produced under pine canopy in the unthinned plots, whereas no differences between canopy types were observed in the thinned plots. Radial increment and tracheid production were mainly affected by tree water status (air and soil humidity, throughfall). The differences of tree water status caused by treatments, and plausibly disparities in tree size and tree-to-tree competition, were the main drivers explaining the patterns observed for radial increment and xylogenesis. Our results suggest that the negative effects of beech competition on Scots pine growth in similar mixed forest may be controlled to some extent by thinning.  相似文献   

A detailed molecular analysis of complete Bovine Leukemia Virus genomes isolated from B-cell lymphosarcomas     

Gonzalo Moratorio  Sabrina Fischer  Sergio Bianchi  Lorena Tomé  Gonzalo Rama  Gonzalo Obal  Federico Carrión  Otto Pritsch  Juan Cristina 《Veterinary research》2013,44(1):19

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Feline trombiculosis: a retrospective study in 72 cats     

Federico Leone  Andrea Di Bella  Antonella Vercelli  Luisa Cornegliani 《Veterinary dermatology》2013,24(5):535-e126

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Thermographic medium-far ground-based proximal sensing for in-field wheat Stagonospora nodorum blotch detection     

Antonucci  Francesca  Menesatti  Paolo  Iori  Angela  Pallottino  Federico  D’Egidio  Maria Grazia  Costa  Corrado 《植物病害和植物保护杂志》2013,120(5-6):205-208

Journal of Plant Diseases and Protection - Thermal imaging is a potential remote sensing tool for estimating fungal wheat diseases. This study for the first time investigated the suitability of...  相似文献