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121.
The existence of genetic control over the abundance of particular taxa and the link of these to energy balance and growth has been documented in model organisms and humans as well as several livestock species. Preliminary evidence of the same mechanisms is currently under investigation in pigs. Future research should expand these results and elicit the extent of genetic control of the gut microbiome population in swine and its relationship with growth efficiency. The quest for a more efficient pig at the interface between the host and its metagenome rests on the central hypothesis that the gut microbiome is an essential component of the variability of growth in all living organisms. Swine do not escape this general rule, and the identification of the significance of the interaction between host and its gut microbiota in the growth process could be a game-changer in the achievement of sustainable and efficient lean meat production. Standard sampling protocols, sequencing techniques, bioinformatic pipelines and methods of analysis will be paramount for the portability of results across experiments and populations. Likewise, characterizing and accounting for temporal and spatial variability will be a necessary step if microbiome is to be utilized routinely as an aid to selection.  相似文献   
122.
The Atlantic salmon (Salmo salar) is a freshwater and marine fish of the family Salmonidae, widely farmed in aquaculture facilities in several countries. The salmon are carnivorous, but in aquaculture, alternative foods have been experienced. It is well known that feeding in captivity should cause adaptation and modifications of the morphological characteristics of the oral cavity, especially of tongue; therefore, the aim of this study was to investigate, by light, laser confocal and scanning electron microscopy, the morphological characteristics of the tongue dorsal surface, considering the importance of the correlations between feeding habits and the anatomy of the tongue. Scanning electron microscopy demonstrates the presence of caniniform teeth with oro-aboral orientation surrounded by numerous filiform papillae, single, fused or arranged in row. Oro-aborally, the papillae show an appearance like a rosette and they disappear at level of the root. Light and laser confocal microscopy demonstrates that the mucosa is covered by a non-keratinized stratified pavement epithelium with, in the deepest layer, the presence of a triangular structure whose apex is cranially directed and base facing aborally. In this structure, spindle-shaped cells are present, with a vimentin immunoreactivity, that for their characteristics could be adult mesenchymal stem cells. The obtained data could be useful not only for further studies on the nutrition, but it is interesting the detection of tissues typical of the embryo-fetal phase in the adult specimens tongue, thus giving a basis for studies of potential applications, if any, regarding cell therapies for different clinical indications.  相似文献   
123.
The use of organic amendments in agriculture could sustain crop production and preserve the agroecosystem, due to their importance in the conservation of organic matter in soil. The objective of this research was to evaluate the effects of differing organic fertilizers and of previous crops on lettuce growth, nutritional status and yields. The effects on soil characteristics were further investigated. The research was carried out during two years (2006 and 2007) at Metaponto (MT) in southern Italy, on lettuce crops cultivated after eggplant and melon to avoid their continuous cropping. The effects of application of a mineral fertilizer (MF), and of three treatments with organic fertilizers (commercial stable manure - OM; anaerobic digestate based on wine distillery wastewater - AD; composted municipal solid organic wastes coming from the separate collection - MSW) were studied. Head average weight, leaf area index, nitrate content and SPAD readings during the cropping cycles did not show significant differences among fertilizer treatments. On the contrary, marketable yield and head average weight at the harvest presented significantly different among the three organic fertilizers. The average marketable yield and head average weight of organic fertilizers experimental (AD and MSW treatments) decreased of 16 and 17%, respectively, compared to OM treatment. The previous melon crop influenced positively all analyzed parameters of the lettuce compared with previous eggplant crop. The previous melon crop reached the highest marketable yield with an increase of 59% compared with previous eggplant crop. Compared to the mineral fertilizer, the organic ones significantly increased the extracted fraction of soil organic carbon (6.9, 10.7 and 14.9% for OM, AD and MSW, respectively), without significant changes for the humic and fulvic content.  相似文献   
124.
Abstract

A short-term pot experiment was made to evaluate the effectiveness of municipal solid waste compost amendment on salt-affected soil. Hordeum maritimum plants were cultivated in pots filled with a clay-loam soil containing 0 or 40 t ha?1 of compost and irrigated with tap water at 0 or 4 g l?1 NaCl. Soil properties and heavy metal (Zn2 +, Pb2 +, Cd2 +) accumulation were investigated. Municipal solid waste compost application significantly increased the soil contents of carbon, nitrogen and potassium under both non-saline and saline conditions. Soil heavy metal concentrations increased substantially too, but the recorded values were below the toxicity limits. Interestingly, plants subjected to the salt–compost interaction were more vigorous, compared with those grown on non-amended soil. Altogether, our data indicate that short-term utilization of municipal solid waste compost at 40 t ha?1 may be of potential interest in the perspective of the rehabilitation of salt-affected soils. Yet, it must be stressed that the present findings are preliminary and need to be further evaluated under field conditions before practical recommendations can be inferred.  相似文献   
125.
Abstract

Composting olive oil pomace could solve the problem of disposal, by recycling this organic waste for agricultural purposes. Furthermore, application of composted organic waste could be a way to sustain both soil fertility and production, especially in organic farming. Therefore, the aim of this research was to evaluate the phytotoxicity and the effects of application of olive pomace composts on emmer performance and soil properties. Two types of olive pomace composts, with a different initial C/N ratio, were stopped at the active phase and processed until maturation. The obtained four olive pomace composts were compared with a commercial fertilizer in a two-year field experiment. Before the field trial, a bioassay was performed to assess phytotoxicity both for the raw pomace and the not-stabilized composts. Growing and yielding data for emmer were determined during the two-year period and soil characteristics were measured at the start and at the end of the experiment.

The composts were not phytotoxic (germination index was higher than 90%) and their total organic carbon content was always higher than the minimum values established by the Italian fertilizers legislation. The emmer protein content was significantly higher in the matured compost treatment with low C/N, than in the other compost treatments. Its value was comparable with that of the commercial fertilizer, suggesting a good performance on crop yield quality. Although not significant, this compost showed an increase of 9.8% in grain yield compared with commercial fertilizer treatment. On the whole, it can be suggested that repeated compost application might preserve the soil organic carbon content and supply macronutrients to a crop.  相似文献   
126.
Eighty-seven Phaseolus vulgaris landraces, still cultivated in Calabria (Italy), were investigated in order to study the patterns of common bean genetic diversity in this region, to better understand the evolutionary development of beans in Europe and to properly manage these genetic resources. Four American accessions and five Italian varieties were also included. Different markers, such as 12 microsatellites, seed traits, phaseolins and 100-seed weight were combined with different statistical approaches. For each microsatellite, expected (H e ) and observed (H o ) heterozygosities, polymorphism information content (PIC), probability of identity (PI) and homozygosity were calculated. Furthermore, in Calabrian group of bean landraces, total (N a ) and private (N pa ) number of alleles, observed (H o ), expected heterozygosities (H e ) and allelic richness (AR) were calculated. Genetic distances among landraces were estimated using Nei’s coefficient and a cluster analysis using the UPGMA algorithm was performed. The results clearly indicated that: (1) Calabrian germplasm showed a high level of diversity (H e  = 0.595); (2) Mesoamerican and Andean gene pools were clearly distinguished in Calabrian germplasm, with the Andean gene pool predominating (83 %); (3) Calabrian landraces were largely hybridized within and between the gene pools. A model-based approach, using the STRUCTURE software, was adopted. Six groups, including 4 of Andean origin and one of Mesoamerican origin were identified. Even more interesting, a small group (8 %) showed a distinct genetic structure, in which interspecific hybridizations with runner bean (Phaseolus coccineus L.) could have occurred. Nevertheless, a relatively high proportion of Calabrian bean landraces (12.6 %) was derived from intra and interspecific hybridizations.  相似文献   
127.
Substantial progress has been made in breaking down the complex pattern of proteins in wheat, and the constant improvement of “‐omics” approaches has allowed relating the abundance of selected wheat proteins to flour and semolina performance during processing. This strategy has provided some sound molecular basis for the work of geneticists, breeders, and growers. Along with an update on work in this area, this review is meant to highlight that performance‐relevant proteins may need specific structural features to carry out performance‐relevant functions during processing. In this frame, some of the approaches used to investigate structural features of wheat proteins are discussed, with a focus on water‐insoluble gluten proteins and on those approaches that allow minimal disturbance of the protein structure itself. Selected examples of the application of some of these approaches to the study of starting materials and of structural modifications that occur at various processing stages are presented. The effects of process‐induced modifications on the various interactions among macromolecules that govern the physical and sensory features of wheat‐based foods are also briefly discussed.  相似文献   
128.

Background  

The serotonergic system is associated with numerous brain functions, including the resetting of the mammalian circadian clock. The synthesis and metabolism of 5-HT in the brain increases in response to exercise and is correlated with high levels of blood-borne tryptophan (TRP). The present investigation was aimed at testing the existence of a daily rhythm of TRP and 5-HT in the blood of athletic horses.  相似文献   
129.
130.
The object of the study was to determine the chemical composition of Diospyros lotus L. extract and their antioxidant and antiproliferative properties. Eight compounds were isolated from D. lotus and identified as gallic acid, methylgallate, ellagic acid, kaempferol, quercetin, myricetin, myricetin 3-O-β-glucuronide, and myricetin-3-O-α-rhamnoside. D. lotus extract tested in different in vitro systems (DPPH, ABTS, FRAP, and Fe2+ chelating activity assay) showed significant antioxidant activity. The potential antiproliferative properties of D. lotus extract and isolated compounds against nine human cancer cell lines such as COR-L23, CaCo-2, C32, ACHN, A375, A549, Huh-7D12, MCF-7, and LNCaP were investigated in vitro by SRB assay. D. lotus extract demonstrated the highest inhibitory activity against COR-L23 with an IC50 value of 12.2 μg/ml. Among identified hydrolysable tannins, ellagic acid evidenced strong antiproliferative activity against both C32 and A375 cells with IC50 values of 0.8 and 4.1 μg/ml, respectively. Interesting results were observed, also, with gallic acid that showed the highest cytotoxic activity against CaCo-2 (IC50 2.6 μg/ml). Overall, the results of this study suggest that D. lotus displays a good antioxidant activity and has antiproliferative effects. Both activities are related to identified phenolic compounds.  相似文献   
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