Effects of vertically transferred 3,3',4,4',5-pentachlorobiphenyl on gene expression in the ovaries of immature Sprague-Dawley rats     

Sakurada Y  Shirota M  Mukai M  Inoue K  Akahori F  Watanabe G  Taya K  Shirota K 《The Journal of reproduction and development》2007,53(4):937-943

We have previously shown that 3,3',4,4',5-pentachlorobiphenyl (PCB-126) vertically transferred from dams potentially exerts a direct effect on the ovaries of offspring and adversely affects female puberty. To investigate its toxicological targets in ovarian tissues, mRNAs encoding representative peptides that regulate follicular development in granulosa cells, theca cells, and oocytes were quantified using ovaries collected on postnatal days (PND) 5, 15, and 24 from the offspring of dams administered oral doses of 0, 1 or 3 microg/kg PCB-126 starting 2 weeks prior to mating and continuing until 20 days after delivery. Quantification using the real-time RT-PCR method revealed that PCB-126 lowered the amounts of mRNAs that encoded the inhibin alpha- and inhibin/activin beta A-subunits from PND 15 onwards; the amounts of mRNAs for inhibin/activin beta B-subunit, follicle-stimulating hormone (FSH) receptor, and aromatase on PND 15; and the amounts of luteinizing hormone receptor mRNA on PND 24 compared with those of the age-matched controls. In contrast, no differences were noted for mRNAs encoding c-kit, growth differentiation factor-9, bone morphogenetic protein-15, or kit ligand for any of the age groups examined. The serum FSH level on PND 24 was higher than that in the control. Since the earliest effects on the mRNAs in the rat ovaries were observed in those expressed in the granulosa cells of the growing follicles after the antral follicles had developed, molecules in granulosa cells but not in oocytes during the early stages of the antral follicles might be the primary targets of vertically transferred PCB-126.  相似文献   

Native and introduced Far Eastern daces,genus Tribolodon,in the Tama River system,Japan, inferred from mitochondrial DNA analysis     

Shirotori  Fumiaki  Okuyama  Fumiya  Ida  Hitoshi  Aoyama  Jun  Yoshinaga  Tatsuki 《Fisheries Science》2020,86(3):473-485

Fisheries Science - To investigate the species composition and genetic characteristics of Far Eastern daces, genus Tribolodon, in the Tama River, Japan, partial DNA sequences of the mitochondrial...  相似文献   

Formaldehyde adsorption by karamatsu (Larix leptolepis) bark     

Toshiyuki Takano  Tomomi Murakami  Hiroshi Kamitakahara  Fumiaki Nakatsubo 《Journal of Wood Science》2008,54(4):332-336

Formaldehyde adsorption and release tests of karamatsu (Larix leptolepis) bark and bark ingredients were performed. Karamatsu bark had good formaldehyde adsorption ability that was better than microcrystalline cellulose. An acetone-soluble fraction from karamatsu bark, which might contain bark tannin, had excellent formaldehyde adsorption ability, and was found to release a trace amount of the adsorbed formaldehyde, suggesting that its formaldehyde adsorption was predominantly due to chemical adsorption. It was confi rmed that the acetone-soluble fraction played an important part in formaldehyde adsorption by karamatsu bark. An acetone-insoluble fraction from karamatsu bark had good formaldehyde adsorption ability as well as the bark, in spite of the residue after the removal of the acetone-soluble fraction.  相似文献   

Azide ion as a quinone methide scavenger in the horseradish peroxidasecatalyzed polymerization of sinapyl alcohol     

Yuki Tobimatsu  Toshiyuki Takano  Hiroshi Kamitakahara  Fumiaki Nakatsubo 《Journal of Wood Science》2008,54(1):87-89

  相似文献   

Estimation of stand volumes using the <Emphasis Type="Italic">k</Emphasis>-nearest neighbors method in Kyushu,Japan     

Tsuyoshi Kajisa  Takuhiko Murakami  Nobuya Mizoue  Fumiaki Kitahara  Shigejiro Yoshida 《Journal of Forest Research》2008,13(4):249-254

The k-nearest neighbors (kNN) method is widely employed in national forest inventory applications using remote sensing data. The objective of this study was to evaluate the kNN method for stand volume estimation by combining LANDSAT/ETM+ data with 622 field sample plots from the Japanese National Forest Inventory (NFI) in Kyushu, Japan. The root mean square error (RMSE) and relative RMSE of the volume estimates rapidly decreased as the number of nearest neighbors (k) increased up to five, and then it slightly declined. They were consistently smaller for the Euclidean distance than for the Mahalanobis distance. The estimation errors (RMSE and relative RMSE) were 169.2 m³/ha and 66.2%, respectively (k = 10). The relative RMSE was similar to the previous studies. The estimated values were more accurate towards the mean value of the total volume, with an overestimation of the low volumes and an underestimation of the high volumes. We found a significant linear relationship between the observed stand volumes and estimated errors, which suggests that systematic errors may be reduced using this linearity. This research concluded that the kNN method is suitable for estimating stand volumes in Kyushu.  相似文献   

Mechanism of formaldehyde adsorption of (+)-catechin   总被引：1，自引：1，他引：0  

Toshiyuki Takano  Tomomi Murakami  Hiroshi Kamitakahara  Fumiaki Nakatsubo 《Journal of Wood Science》2008,54(4):329-331

The reaction of (+)-catechin with formaldehyde vapor was investigated as a model reaction of formaldehyde adsorption of (+)-catechin. It was found by H nuclear magnetic resonance spectroscopy, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and gel permeation chromatography of the product that the vapor reaction of (+)-catechin with formaldehyde without a solvent or a catalyst proceeds via methylolation, condensation, and polymerization. This is the same as the reaction in a solvent in the presence of an acid or a base. The formation of polyoxymethylenes such as paraformaldehyde was not confirmed.  相似文献   

A draft sequence of the rice genome (Oryza sativa L. ssp. japonica)   总被引：4，自引：0，他引：4  

Goff SA  Ricke D  Lan TH  Presting G  Wang R  Dunn M  Glazebrook J  Sessions A  Oeller P  Varma H  Hadley D  Hutchison D  Martin C  Katagiri F  Lange BM  Moughamer T  Xia Y  Budworth P  Zhong J  Miguel T  Paszkowski U  Zhang S  Colbert M  Sun WL  Chen L  Cooper B  Park S  Wood TC  Mao L  Quail P  Wing R  Dean R  Yu Y  Zharkikh A  Shen R  Sahasrabudhe S  Thomas A  Cannings R  Gutin A  Pruss D  Reid J  Tavtigian S  Mitchell J  Eldredge G  Scholl T  Miller RM  Bhatnagar S  Adey N  Rubano T  Tusneem N  Robinson R  Feldhaus J  Macalma T  Oliphant A 《Science (New York, N.Y.)》2002,296(5565):92-100

The genome of the japonica subspecies of rice, an important cereal and model monocot, was sequenced and assembled by whole-genome shotgun sequencing. The assembled sequence covers 93% of the 420-megabase genome. Gene predictions on the assembled sequence suggest that the genome contains 32,000 to 50,000 genes. Homologs of 98% of the known maize, wheat, and barley proteins are found in rice. Synteny and gene homology between rice and the other cereal genomes are extensive, whereas synteny with Arabidopsis is limited. Assignment of candidate rice orthologs to Arabidopsis genes is possible in many cases. The rice genome sequence provides a foundation for the improvement of cereals, our most important crops.  相似文献   

Epidemiology of jockey falls and injuries in flat and jump races in Japan (2003–2017)     

Fumiaki MIZOBE  Yuji TAKAHASHI  Kanichi KUSANO 《Journal of Equine Science》2020,31(4):101

  相似文献   

Size-exclusion chromatography of tea tannins and intercepting potentials of peptides for the inhibition of trypsin-caseinolytic activity by tea tannins     

Kasai N  Nakatsubo G 《Journal of agricultural and food chemistry》2006,54(14):5149-5156

Molecular-weight distribution and characterization of tea tannin were investigated by high-performance liquid chromatography and the equivalent preparative exclusion gel chromatography using Sephadex G-25. The characteristics of the fractions were studied regarding the amounts of terminal catechin, sugar, and gallic acid, the color reaction of the Folin-Chiocalteu reagent, the UV absorbance, and the inhibition activity for the trypsin-caseinolytic activity per weight. Furthermore, we investigated the intercepting activities of the inhibition by the amino acids, peptides, their analogues, poly(ethylene glycol)s (PEGs), and histatin 5 using the inhibition of trypsin-caseinolytic activity by tea. Arg, Lys, and their peptides had strong intercepting activities for the inhibition, but only a weak activity was detected in the Pro peptides or gelatin-like peptides of (Pro-Pro-Gly)(n) (n = 5 or 10). The guanidyl group of Arg and the amino methylene group of Lys were important for the intercepting activity, but the activity was weakly dependent upon the peptide bond formation. The intercepting activity of the peptides or PEG exponentially increased with the number of polymerizations. Histatin 5 did not have a remarkably strong intercepting activity considering the peptide length. The activity of the synthetic histatin 5 in which all of the Lys and Arg were substituted by Ala was at the same level as histatin 5.  相似文献   

Growth and root sucker ability of field-grown transgenic poplars overexpressing xyloglucanase     

Toru Taniguchi  Ken-ichi Konagaya  Manabu Kurita  Naoki Takata  Katsuaki Ishii  Teiji Kondo  Fumiaki Funahashi  Seiichi Ohta  Tomomi Kaku  Kei’ichi Baba  Rumi Kaida  Takahisa Hayashi 《Journal of Wood Science》2012,58(6):550-556

Xyloglucan is thought to be a key hemicellulose cross-linking adjacent cellulose microfibrils in plant cell walls. The growth traits of transgenic poplars (Populus alba) with decreased xyloglucan from overexpression of Aspergillus aculeatus xyloglucanase were characterized during a 4-year field trial. The field-trial site consisted of two blocks, a fertile soil block and a non-fertile soil block, determined by soil analysis. In the fertile block, the growth of aboveground biomass of the transgenic poplars was reduced to 24?C44?% compared to that of wild-type poplars, in contrast to the growth seen in chamber and greenhouse conditions. In the non-fertile block, the aboveground biomass of transgenic poplars was also smaller than that of the wild-type poplars. Because poplars reproduce asexually by root suckers, we also compared the formation of root suckers from transgenic and wild-type poplars. Root suckers formed less frequently from transgenic poplars than from wild-type poplars. The growth rates of root suckers from transgenic poplars were also slower than those from wild-type poplars. The results showed that constitutive degradation of xyloglucan impairs poplar growth and vegetative reproduction ability.  相似文献