An ELISA with Brucella lipopolysaccharide antigen for the diagnosis of B. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival B. melitensis strain Rev 1 vaccination.     

M P Jiménez de Bagüés  C M Marín  J M Blasco  I Moriyón  C Gamazo 《Veterinary microbiology》1992,30(2-3):233-241

An indirect enzyme-linked immunosorbent assay (ELISA) with unpurified Brucella melitensis smooth lipopolysaccharide (S-LPS) as antigen was evaluated for the serological diagnosis of B. melitensis infection in sheep in comparison with the Rose Bengal (RB), complement fixation (CF), radial immunodiffusion (RID), microplate agglutination (MA) and rivanol agglutination (RIV) tests. Tests RB and CF detected as positive each of the 77 sera from B. melitensis-infected animals tested, the RID (74), MA (76) and the RIV (72) were less sensitive. However, all tests compared were negative when 77 sera from Brucella-free rams were tested. While subcutaneous Rev 1 vaccination induced high response levels in any of the tests, low level responses were obtained upon conjunctival vaccination, particularly in ELISA and RID tests.  相似文献   

Studies on the fine structure of the rostellar hooks of Multiceps endothoraciccus during the ultimate phase of their formation     

D Hulínská  V M Fedoseenko 《Folia parasitologica》1977,24(4):347-351

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The effect of hormonal stimulation on the follicle population in the ovaries of ewes during estrus and anestrus     

J Arendarcik  M Molnárová  A Staníková 《Veterinární medicína》1987,32(10):611-617

In the oestral and anoestral periods, 129 ewes of the Slovak Merino breed were treated with different doses of the stimulants PMSG, PGF2 alpha, HCG, SG and FSH after synchronization by means of Ageline tampons. In the anoestral period, PMSG doses of 750 and 1000 i. u. were found to increase the number of tertiary follicles from 12.3 to 17.2 and 18.6, whereas the dose of 1500 i. u. was found to reduce this number from 18.6 to 13.5. PGF2 alpha increases the average number of tertiary follicles from 12.3 to 22.2 and when it is combined with 750 i. u. PMSG, the increase is to 29.3 tertiary follicles. At higher PMSG doses the ratio of atretic (A) to non-atretic (N) tertiary follicles rose and the number of ovulations increased from 2.5 to 4.0. After synchronization with Ageline and administration of PMSG at the doses of 750 and 1000 i. u., the number of tertiary follicles increased from 16.0 to 18.4 and the number of ovulations from 3.1 to 5.7; the number of atretic tertiary follicles showed no change. In the oestral period, the dose of 1000 i. u. PMSG administered after PGF2 alpha reduced the number of tertiary follicles from 18.9 to 14.5 and increased the number of ovulation from 1.6 to 2.6; an increase was also recorded in the number of atretic tertiary follicles.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Characteristics of the meat of cattle with cysticercosis due to experimental infection with the eggs of the tapeworm Taenia saginata and treated with praziquantel     

J Gilka  J Habrda  B Janková  P Krejcí  Z Matyás 《Veterinární medicína》1983,28(5):257-268

Eleven test and control bullocks of the Bohemian Spotted breed were studied for the effect of cysticercosis and their treatment with praziquantel on the properties of meat and fat. As found, the disease affected the composition of meat and fat and some of the studied characteristics and technological properties of meat. Although the measles disappeared after treatment, they were not resorbed. Therefore, irrespective of the worsened meat quality, the meat and organs of such animals cannot be evaluated in any other way but according to the valid veterinary regulations.  相似文献   

Meat quality in broilers affected with inflammation of the feather follicles     

B Janková  J Gilka  S Standara  P Krejcí  V R?zicková 《Veterinární medicína》1987,32(9):563-570

Broilers with feather follicle inflammation and birds free of this disorder were selected from the broiler chickens kept on plastic (bralen-)coated metallic slats. Both groups of broilers were killed on a sanitary slaughter line and the samples of breast and thigh muscles were analyzed for the basic composition and characteristics of the metabolism of nitrogenous and lipidic components. The content of individual amino acids in the muscles and the proportion of fatty acids in the intramuscular fat of broilers were determined for the evaluation of nutritive value. The samples of the affected spots of skin and samples of organs (liver) were subjected to microbiological examination. The resultant finding represented the common mesophilous microflora. No substantial statistically significant differences in chemical characteristics were found between the two groups. It follows from the results that the inflammation of feather follicles is a local skin disorder with no effect on the quality of the meat.  相似文献   

Catalase activity in various types of bovine and porcine adipose tissue     

O Zatocil  S Stíchová  F Stícha 《Veterinární medicína》1974,47(7):397-406

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Toxoplasmosis in the muscrat Ondatra zibethica (Linné) 1766 and in the stone marten Martes foina (Erxleben) 1777     

K Rasín 《Veterinární medicína》1971,16(4):255-260

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Evaluation of Duddingtonia flagrans in reducing infective larvae of Haemonchus contortus in feces of sheep.     

M T Pe?a  J E Miller  M E Fontenot  A Gillespie  M Larsen 《Veterinary parasitology》2002,103(3):259-265

Consequences of nematode infections due to Haemonchus contortus are a serious constraint for the sheep industry worldwide. Development of anthelmintic resistance and increasing concern about the impact of anthelmintic use dictate the need of alternative control. Such an alternative is using the nematode trapping fungus Duddingtonia flagrans to reduce infective larvae levels on pasture. Two trials were conducted to determine the effect of D. flagrans in reducing infective larvae (predominantly H. contortus) in feces. The first trial determined the dose effect of D. flagrans in reducing infective larvae in feces. Eighteen ewes were dewormed to remove existing infections and randomly assigned to six treatment groups: 5 x 10(4), 1 x 10(5), 2.5 x 10(5), 5 x 10(5), 1 x 10(6) or no (control) spores of D. flagrans per kg of body weight mixed in their feed for 7 days. Fecal samples were collected daily from these and from infected donor ewes. Feces from individual-treated ewes were mixed with equal amounts of donor ewe feces, theoretically approximating oral dose spore concentrations of 2.5 x 10(4), 5 x 10(4), 1.25 x 10(5), 2.5 x 10(5), 5 x 10(5) and no spores, and were cultured. Across dosages and during the 7 days of fungus feeding, percent reduction of infective larvae ranged from 76.6 to 100.0%. The second trial determined the effect of D. flagrans at the dose of 10(5) spores per kg body weight on reducing infective larvae in feces from naturally infected lambs. Twenty lambs were randomly assigned to either treatment or control groups based on fecal egg count. Treatment lambs were fed spores mixed in feed for 7 days. Feces were collected daily and cultured. During the 7 days of fungus feeding, the percent reduction of infective larvae ranged from 82.8 to 99.7%. Results of these trials demonstrated that the nematode trapping fungus D. flagrans was highly effective in reducing infective larvae in sheep feces and should be considered as a biological control agent for integrated nematode control programs.  相似文献   

Experimental poisoning of pheasant chicks with nitrates and nitrites in drinking water     

Z Strnad  M Persín 《Veterinární medicína》1983,28(9):541-547

The effect was studied of nitrates and nitrites in drinking water on the methemoglobin level in blood and pathomorphological changes in fourteen-day-old pheasant chickens. The concentrations of 500 ppm of NO3- and 15 ppm NO2- in the drinking water were not lethal, they caused only the increase in the methemoglobin in blood to 7.1% (NO3-) and 16.5% (NO2-). The pheasants exposed to NO3- suffered from hyperaemia of liver, kidneys and mucosa of the small intestine and from the multiplication of the eosinophilic granulocytes in the villus stroma. The exposition to NO2- resulted in the non-specific dystrophic changes in liver and kidneys and in the villus edema of the small intestine. Lethal levels of nitrates and nitrites in drinking water were estimated in relation to the age of pheasants.  相似文献   

Effects of growth curve parameters on cow efficiency.     

G López de Torre  J J Candotti  A Reverter  M M Bellido  P Vasco  L J García  J S Brinks 《Journal of animal science》1992,70(9):2668-2672

Weight-age data from 50 Retinta beef cows from 8 to 97 mo of age located in southwestern Spain were fitted to von Bertalanffy, Brody, and Richards functions to determine the relationship between growth curve parameters and cow efficiency. Only cows having at least 31 weights were included in the analysis. Von Bertalanffy, Brody, and Richards functions were fitted to weights of each cow. Relevant parameters of the three functions are A and K, associated with the asymptotic mature weight and rate of maturing, respectively. Criteria for comparisons among the three functions were computing difficulty, goodness of fit, and lack of bias of A. Productivity indicators were number of calves weaned during the first five calving seasons (NC), average birth weight (BWT), average weaning weight (WW), and average weaning weight per cow per year (WWY). The von Bertalanffy function was selected as the most appropriate. Least squares means for A and K were 650 +/- 8.17 kg and .038 +/- .001 mo-1, respectively. The values of NC, BWT, WW, and WWY were 4.0 +/- .11 calves, 38.2 +/- .4 kg, 218 +/- 5 kg, and 172 +/- 5 kg, respectively. Regression analysis for A indicated a decrease in NC when mature weight increased (P less than .05). There was a nonsignificant trend for heavier cows (higher A) to have calves with heavier BWT or WW. The value of WWY increased (P less than .05) with increased maturing rate (K) of cows. No significant associations were found between K and BWT or WW.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献