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A study was carried out to investigate the effects of different diets for heifers, low- and high-yielding cows on the microbial composition of their faeces and subsequently the impacts of these faeces on CO2 and N2O emissions, N mineralisation and plant N uptake. A diet low in N and high in acid detergent fibre offered to heifers resulted in faeces dominated by fungi. These faeces were characterised by a low content in microbial biomass C and N and a high ergosterol concentration in comparison to the faeces of high-yielding cows. Added to soil, faeces of heifers led to lower emission and stronger N immobilisation during a 14-day incubation in comparison to the faeces of high-yielding cows. Total N2O emission was significantly (P?<?0.05) correlated with faecal microbial biomass N. Rye grass yield and N uptake were lowest in the soil supplemented with faeces from heifers in a 62-day pot experiment. Plant N uptake was influenced by the faecal microbial biomass C/N ratio and the fungal C to bacterial C ratio. In conclusion, the faecal microbial biomass was affected to a high degree by the feeding regime and faecal microbial characteristics revealed higher impacts on plant N uptake than soil microbial properties.  相似文献   
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Breeding for organic agriculture: the example of winter wheat in Austria   总被引:2,自引:2,他引:0  
Breeding for organic agriculture (BFOA) is a strategy for a commercial breeding company based on the exploitation of the frequently observed high correlation for many traits between conventional, low input (LI) and organic agriculture (ORG). Indirect selection under LI can be useful to roughly divide the germplasm into the genotypes better adapted to high input and those better adapted to LI or ORG conditions. BFOA is an evolving process, in which two methods are currently applied: early generations are either selected following the pedigree system under LI, or grown as bulk populations on ORG fields. In the latter case the system switches to LI after individual ear selection under ORG conditions. In both methods, the first replicated yield trial is grown parallel under ORG and LI. Subsequently, the genotypes are grouped into conventional or ORG advanced trial series. The BFOA strategy allows that the larger genetic variability of both the organic and conventional gene pool can be exploited in the selection for ORG. Hitherto, seven winter wheat varieties were released in Austria after exclusive organic VCU testing.  相似文献   
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Excessive nitrogen (N) fertilizer application is very common in the North China Plain. Diagnosis of in-season N status in crops is critical for precision N management in this area. Remote sensing, as a timely and nondestructive tool, could be an alternative to traditional plant testing for diagnosing crop N status. The objectives of this study were to determine which vegetation indices could be used to estimate N status in winter wheat (Triticum aestivum L.) under high N input conditions, develop models to predict winter wheat N uptake using spectral vegetation indices and validate the models with data from farmers’ fields. An N rate experiment and a variety-N experiment were conducted in Huimin, Shandong Province from 2005/2006 to 2006/2007 to develop the models. Positive linear relationships between simple ratio vegetation indices (red vegetation index, RVI and green vegetation index, GVI) and N uptake were observed independent of growth stages and varieties (R2, 0.48–0.74). In contrast, the relationships between normalized difference vegetation indices (NDVI and GNDVI), red and green normalized difference vegetation index (RGNDI), and red and green ratio vegetation index (RGVI) were exponentially related to N uptake (R2, 0.43–0.79). Subsequently, 69 farmers’ fields in four different villages were selected as datasets to validate the developed models. The results indicated that the prediction using RVI had the highest coefficient of determination (R2, 0.60), the lowest root mean square error (RMSE, 39.7 kg N ha−1) and relative error (RE, 30.5%) across different years, varieties and growth stages. We conclude that RVI can be used to estimate nitrogen status for winter wheat in over-fertilized farmers’ fields before heading.  相似文献   
85.
BACKGROUND: In the present study the principle of energy dispersive X‐ray microanalysis (EDX), i.e. the detection of elements based on their characteristic X‐rays, was used to localise and quantify organic and inorganic pesticides on enzymatically isolated fruit cuticles. Pesticides could be discriminated from the plant surface because of their distinctive elemental composition. RESULTS: Findings confirm the close relation between net intensity (NI) and area covered by the active ingredient (AI area). Using wide and narrow concentration ranges of glyphosate and glufosinate, respectively, results showed that quantification of AI requires the selection of appropriate regression equations while considering NI, peak‐to‐background (P/B) ratio, and AI area. The use of selected internal standards (ISs) such as Ca(NO3)2 improved the accuracy of the quantification slightly but led to the formation of particular, non‐typical microstructured deposits. The suitability of SEM‐EDX as a general technique to quantify pesticides was evaluated additionally on 14 agrochemicals applied at diluted or regular concentration. CONCLUSION: Among the pesticides tested, spatial localisation and quantification of AI amount could be done for inorganic copper and sulfur as well for the organic agrochemicals glyphosate, glufosinate, bromoxynil and mancozeb. Copyright © 2009 Society of Chemical Industry  相似文献   
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