Physiological and biochemical differences among Ulmus minor genotypes showing a gradient of resistance to Dutch elm disease          下载免费PDF全文

M. Li  R. López  M. Venturas  J. A. Martín  J. Domínguez  G. G. Gordaliza  L. Gil  J. Rodríguez‐Calcerrada 《Forest Pathology》2016,46(3):215-228

Dutch elm disease (DED) spread across Europe and North America in the 20th century killing most natural elm populations. Today, breeding programmes aim at identifying, propagating and studying elm clones resistant to DED. Here, we have compared the physiology and biochemistry of six genotypes of Ulmus minor of variable DED resistance. Leaf gas exchange, water potential, stem hydraulic conductivity and biochemical status were studied in 5‐year‐old trees of AB‐AM2.4, M‐DV2.3, M‐DV2 × M‐CC1.5 and M‐DV1 and 6‐year‐old trees of VA‐AP38 and BU‐FL7 before and after inoculation with Ophiostoma novo‐ulmi. Leaf water potential and net photosynthesis rates declined, while the percentage loss of hydraulic conductivity (PLC) increased after the inoculation in susceptible trees. By the 21st day, leaf predawn and midday water potential, stomatal conductance to water vapour and net photosynthesis rates were lower, and PLC was higher in trees of susceptible (S) genotypes inoculated with the pathogen than in control trees inoculated with water, whereas no significant treatment effect was observed on these variables in the resistant (R) genotypes. Fourier transform infrared spectroscopy analyses revealed a different biochemical profile for branches of R and S clones. R clones showed higher absorption peaks that could be assigned to phenolic compounds, saturated hydrocarbons, cellulose and hemicellulose than S clones. The differences were more marked at the end of the experiment than at the beginning, suggesting that R and S clones responded differently to the inevitable wounding from inoculation and repeated sampling over the experimental course. We hypothesize that a weak activation of the defence system in response to experimental wounding can contribute to the susceptibility of some genotypes to O. novo‐ulmi. In turn, the decline in shoot hydraulic conductivity and leaf carbon uptake caused by the infection further exacerbates tree susceptibility to the fungus.  相似文献   

Rescuing agroforestry as strategy for agriculture in Southern Brazil     

Alexandre Siminski  Karine Louise dos Santos  Juliano Gil Nunes Wendt 《林业研究》2016,27(4):739-746

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Identification of the target region including the Foc01/foc01 gene and development of near isogenic lines for resistance to Fusarium Wilt race 0 in chickpea     

W. Jendoubi  M. Bouhadida  T. Millan  M. Kharrat  J. Gil  J. Rubio  E. Madrid 《Euphytica》2016,207(1):119-133

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Silver catfish Rhamdia quelen immersion anaesthesia with essential oil of Aloysia triphylla (L'Hérit) Britton or tricaine methanesulfonate: effect on stress response and antioxidant status     

Luciane Tourem Gressler  Ana Paula Konzen Riffel  Thaylise Vey Parodi  Etiane Medianeira Hundertmarck Saccol  Gessi Koakoski  Sílvio Teixeira da Costa  Maria Amália Pavanato  Berta Maria Heinzmann  Bráulio Caron  Denise Schmidt  Susana Francisca Llesuy  Leonardo José Gil Barcellos  Bernardo Baldisserotto 《Aquaculture Research》2014,45(6):1061-1072

Responses to anaesthesia with essential oil (EO) of Aloysia triphylla (135 and 180 mg L^?1) and tricaine methanesulfonate (MS222) (150 and 300 mg L^?1) were assessed in silver catfish. Exposure to the anaesthetics elicited a stress response in the species. In the case of MS222, it was displayed as a release of cortisol into bloodstream, elevation in hematocrit and plasma ion loss. The EO presented cortisol‐blocking properties, but increased haematocrit and disturbances of hydromineral balance were observed. Liver antioxidant/oxidant status of EO and MS222‐anaesthetized silver catfish was also estimated. The synthetic anaesthetic induced lipoperoxidation, notwithstanding increased catalase contents, whereas the naturally occurring product was capable of preventing the formation of lipid peroxides, possibly due to combined actions of catalase and glutathione‐S‐transferase. Anaesthetic efficacy was also tested via induction and recovery times. Overall, the promising results obtained for the physiological parameters of the EO‐treated fish counterbalanced the slight prolonged induction time observed for 180 mg L^?1. As for 135 mg L^?1, both induction and recovery times were lengthy; despite that, the EO was able to promote oxidative protection and mitigate stress. None of the MS222 concentrations prompted such responses concomitantly.  相似文献   

A Mediterranean japonica rice (Oryza sativa) cultivar improvement through anther culture   总被引：1，自引：0，他引：1  

X. Serrat  M. Cardona  J. Gil  A. M. Brito  L. Moysset  S. Nogués  E. Lalanne 《Euphytica》2014,195(1):31-44

Certified seed producers systematically select and propagate registered varieties year after year in order to maintain their uniformity and the original registered cultivar traits. However, natural mutations, spontaneous breeding between varieties and alien grain contamination can introduce undesirable variability. NRVC 980385 is a temperate japonica rice cultivar (Oryza sativa ssp. japonica) first registered in Spain in 2002. In 2005 certification tests detected a plot differing from the original traits in terms of uniformity and height suggesting the presence of a certain heterozygosis. This material was therefore seen as an opportunity to obtain newly stabilized doubled haploid (DH) lines which could compete in the Spanish short grain seed market. In this study, an in vitro anther culture protocol is defined which also covers the field tests selection to obtain four new, improved and stabilized DH derived lines ready to be registered for commercial proposes. This took just 4 years from the initial anther collection until new lines were grown in large scale field trials. Consequently, this protocol reduces the time for obtaining field assessed DH lines thereby having considerable advantages over other techniques by both maintaining the original registered cultivars and/or generating new derived varieties.  相似文献   

Iris abscesses with and without intralenticular fungal invasion in the horse     

Dennis E. Brooks  David P. Taylor†  Caryn E. Plummer  Rick Quinn‡  Maria E. Kallberg  Barbara Sheppard†  Kathleen P. Barrie  Sarah E. Blackwood  Catherine M. Nunnery  Gil Ben-Shlomo  Carol J. Clark§  Avery A. Woodworth 《Veterinary ophthalmology》2009,12(5):306-312

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CT-soft tissue window of the cranial abdomen in clinically normal dogs: an anatomical description using macroscopic cross-sections with vascular injection     

Rivero MA  Vázquez JM  Gil F  Ramírez JA  Vilar JM  De Miguel A  Arencibia A 《Anatomia, histologia, embryologia》2009,38(1):18-22

The aim of this study was to provide a detailed anatomic atlas of the cranial abdomen by means of computed tomography (CT). Three mature dogs, all mixed breed males, were used. The dogs were sedated, anaesthetized and positioned in sternal recumbency. CT scans from the eighth thoracic vertebra to the fourth lumbar vertebra were performed using a third-generation equipment (TOSHIBA 600HQ scanner) with 1 cm slice thickness. CT-images of the cranial abdomen were taken with soft-tissue window (WL: −14, WW: 658) settings. Dogs were killed and vascular-injection technique was performed: red and blue latex filled the vascular system. Injected dogs were frozen in the same position as used for CT examination and sectioned with an electric bandsaw at 1-cm-thick intervals. The cuts matched as closely as possible to the CT-images. The anatomic sections were compared and studied with the corresponding CT-images, and clinically relevant abdominal anatomic structures were identified and labelled on the corresponding CT-images. The results of our study could be used as a reference for evaluating CT-images of the canine cranial abdomen with abdominal diseases.  相似文献   

Effect of magnesium sulphate and l-tryptophan and genotype on the feed intake, behaviour and meat quality of pigs     

N. Panella-Riera  A. Velarde  A. Dalmau  E. Fbrega  M. Font i Furnols  M. Gispert  J. Soler  J. Tibau  M.A. Oliver  M. Gil 《Livestock Science》2009,124(1-3):277-287

Sixty-nine entire male pigs with different halothane genotype (homozygous halothane positive — nn-, n = 36; and homozygous halothane negative — NN-, n = 33) were fed with a supplementation of magnesium sulphate (Mg) and/or l-tryptophan (Trp) in the diet for 5 days before slaughter. Animals were housed individually and were submitted to stressful ante mortem conditions (mixed in the lorry according to treatments and transported 1 h on rough roads). Individual feed intake was recorded during the 5-d treatment. At the abattoir, pig behaviour was assessed in the raceway to the stunning system and during the stunning period by exposure to CO₂. Muscle pH, colour, water holding capacity, texture and cathepsin activities were determined to assess meat quality. The number of pigs with an individual feed intake lower than 2 kg/day was significantly different among diets (P < 0.05; Control: 8.7%; Mg&Trp: 43.5%; Trp: 17.4%) and they were considered to have inadequate supplement intake. During the ante mortem period, 15.2% of pigs included in the experiment died, and this percentage decreased to 8.7% in those pigs with a feed intake > 2 kg/day, all of them from the stress-sensitive pigs (nn). In general, no differences were observed in the behaviour of pigs along the corridor leading to the stunning system and inside the CO₂ stunning system. During the stunning procedure, Trp diet showed shorter periods of muscular excitation than control and Mg&Trp diets. The combination of a stressful ante mortem treatment and Mg&Trp supplementation led to carcasses with high incidence of severe skin lesions. Different meat quality results were found when considering all pigs or considering only those with adequate supplement intake. In this later case, Trp increased pH₄₅ (6.15) vs Control diet (5.96) in the Longissimus thoracis (LT) muscle (P < 0.05) and pH at 24 h (Trp: 5.59 vs C: 5.47) led to a higher incidence of dark, firm and exudative (DFD) traits in SM muscle (P < 0.05). Genotype affected negatively all the meat quality traits. Seventy-five percent of LT and 60.0% of the SM muscles from nn pigs were classified as pale, soft and exudative (PSE), while none of the NN pigs showed these traits (P < 0.0001). No significant differences were found between genotypes on the incidence of DFD meat.Due to the negative effects observed in the Mg&Trp group in feed intake and carcass quality, the utilization of a mixture of magnesium sulphate and tryptophan is not recommended.  相似文献   

Mechanism and molecular markers associated with rust resistance in a chickpea interspecific cross (Cicer arietinum × Cicer reticulatum)     

E. Madrid  D. Rubiales  A. Moral  M. T. Moreno  T. Millán  J. Gil  J. Rubio 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(1):43-53

A gene that controls resistance to chickpea rust (Uromyces ciceris-arietini) has been identified in a recombinant inbred line (RIL) population derived from an interspecific cross between Cicer arietinum (ILC72) × Cicer reticulatum (Cr5-10), susceptible and resistant to rust, respectively. Both parental lines and all RILs displayed a compatible interaction but differed in the level of infection measured as Disease Severity (DS) and Area Under the Disease Progress Curve (AUDPC). Histological studies of the seedlings of resistant parental Cr5-10 line revealed a reduction in spore germination, appressorium formation, number of haustoria per colony and colony size, with little host cell necrosis, fitting the definition of partial resistance. A Quantitative Trait Locus (QTL) explaining 31% of the total phenotypic variation for DS in seedlings and 81% of the AUDPC in adult plants in the field was located on linkage group 7 of the chickpea genetic map. The AUDPC displayed a bimodal distribution with high frequency of susceptible lines and both the AUDPC and markers showed the same distorted segregation. Consequently, it was hypothesised that a single dominant gene (proposed as Uca1/uca1) controlled resistance to rust in adult plants. This allowed us to locate the gene on the genetic linkage map. Two Sequence Tagged Microsatellite Sites (STMS) markers, TA18 and TA180 (3.9 cM apart) were identified that flank the resistance gene. These findings could be the starting point for a Marker-Assisted Selection (MAS) programme for rust resistance in chickpea.  相似文献   

Assessing GNSS correction signals for assisted guidance systems in agricultural vehicles     

M. Pérez-Ruiz  J. Carballido  J. Agüera  J. A. Gil 《Precision Agriculture》2011,12(5):639-652

Accuracy levels achieved with differential global positioning system (DGPS) receivers in agricultural operations depend upon the quality of the correction signal. This study has assessed differential signal error from a Dedicated Base Station, OmniSTAR VBS, European Geostationary Navigation Overlay System, European reference frame-IP for internet protocol (EUREF-IP) and radio navigation satellite aided technique (RASANT). These signals were utilized in guidance assisting systems for agricultural applications, such as tillage, harvesting, planting and spraying, in which GPS receivers were used under dynamic conditions. Simulations of agricultural operations on different days and at different time slots and simultaneously recording the tractor′s geo-position from a DGPS receiver and the tractor′s geo-position from a real-time kinematic (RTK) GPS allowed the comparison of the GPS correction signals. The hardware used for tractor guidance was a lightbar (Trimble model EZ-Guide Plus) system. ANOVA statistics showed a significant difference between the accuracy of the correction signals from different sources. GPS correction signal recommendations to farmers depend upon the accuracy required for the specific operation: (a) Yield monitoring and soil sampling (<1 m) are possible with all the GPS correction signals accessed in any time slot. (b) Broadcast seeding, fertilizer and herbicide application (<0.5 m) are possible for 80% of time with OmniSTAR VBS, 40% of time with RASANT and EUREF-IP and 100% of time with a dedicated base station. (c) Transplanting and drill seeding (<0.04 m) are not possible with the accuracy correction provided by any one of the systems used in this study.  相似文献